MD05G1183000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012747
Physical Location & Seq
Reverse (-)
31164141 .. 31165306
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1183000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGAACGAACTAATTACACCACATGTAGTTTCTATAGTGAAACTATATGAATTAGGAGCCCGAAGAGTGTTGGTGACTAGTACAGGCTCATTGGGCTGTGTTCCATCTGAGCTAGCCCAAAGGAGTAGAAATGGTGAGTGCGTCGCGGAACTACAAAATGCAGCAGCAATCTTCAACCCACAACTCGTCCAGATGATTCAAGGACTCAACAGGCAACTCGGTTCAAATGTATTTATCACTGCTAATGCATTTGATATGAACATGGACTTCATCTCCAACCCCGAAAAATCTGGTTTTGTGACATCAAAAGTGGCATGTTGTGGGCAAGGACGCTTTAATGGGTTGGGACAGTGCAATCCAACGTCAAACCTGTGCCCAAACAGGGATCTCTATGCTTTCTGGGATGCTTTCCATCCAACTGAGAAAGCAAACAGGCTAATTGTGAAGCAGATTATGTCCGGGTCTAGCAAGTACATGAACCCAATGAACCTAAGCACCATGATGGCTTTGGACTCAATGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

175

Amino Acids

19.05

Weight (kDa)

8.25

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 15 - 151 1.3e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 146
AciI CCGC 1 cut(s) 146
AclWI GGATC 1 cut(s) 393
AfaI GTAC 2 cut(s) 82, 473
AfiI CCNNNNNNNGG 1 cut(s) 382
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 3 cut(s) 175, 200, 225
AhlI ACTAGT 1 cut(s) 77
AluBI AGCT 2 cut(s) 112, 522
AluI AGCT 2 cut(s) 112, 522
AlwI GGATC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 161, 164
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 2 cut(s) 85, 146
AsuNHI GCTAGC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 173, 176
BccI CCATC 3 cut(s) 112, 420, 496
BcnI CCSGG 1 cut(s) 460
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 3 cut(s) 78, 113, 465
BfmI CTRYAG 1 cut(s) 33
BisI GCNGC 2 cut(s) 162, 165
BlsI GCNGC 2 cut(s) 163, 166
Bme1390I CCNGG 1 cut(s) 460
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 1 cut(s) 394
BmtI GCTAGC 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 382
BseGI GGATG 2 cut(s) 409, 412
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMII CTCAG 2 cut(s) 99, 411
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 2 cut(s) 173, 176
Bsh1236I CGCG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 61, 377
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 100, 412
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 116
BspPI GGATC 1 cut(s) 393
BssMI GATC 1 cut(s) 385
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 108, 420, 491
BstF5I GGATG 2 cut(s) 409, 412
BstFNI CGCG 1 cut(s) 146
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNSI RCATGY 2 cut(s) 26, 318
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 173, 176
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BtsCI GGATG 2 cut(s) 409, 412
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 237, 356
Cac8I GCNNGC 1 cut(s) 114
CseI GACGC 2 cut(s) 130, 339
Csp6I GTAC 2 cut(s) 81, 472
CviAII CATG 5 cut(s) 23, 262, 315, 475, 499
CviJI RGCY 8 cut(s) 59, 87, 96, 112, 116, 436, 506, 522
CviKI_1 RGCY 8 cut(s) 59, 87, 96, 112, 116, 436, 506, 522
CviQI GTAC 2 cut(s) 81, 472
DdeI CTNAG 3 cut(s) 108, 420, 491
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 5 cut(s) 26, 265, 318, 478, 502
FaqI GGGAC 1 cut(s) 360
FatI CATG 5 cut(s) 22, 261, 314, 474, 498
Fnu4HI GCNGC 2 cut(s) 162, 165
FokI GGATG 2 cut(s) 399, 416
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 162, 165
FspBI CTAG 3 cut(s) 78, 113, 465
GluI GCNGC 2 cut(s) 162, 165
HapII CCGG 1 cut(s) 459
HgaI GACGC 2 cut(s) 130, 339
Hin1II CATG 5 cut(s) 26, 265, 318, 478, 502
HinfI GANTC 3 cut(s) 196, 204, 512
HpaII CCGG 1 cut(s) 459
HphI GGTGA 2 cut(s) 85, 146
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 146
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 161, 248, 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpyF3I CTNAG 3 cut(s) 108, 420, 491
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 5 cut(s) 26, 265, 318, 478, 502
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 9 cut(s) 69, 196, 203, 276, 367, 383, 385, 418, 472
Lsp1109I GCAGC 2 cut(s) 173, 176
LweI GCATC 1 cut(s) 394
MaeI CTAG 3 cut(s) 78, 113, 465
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 73, 298
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 2 cut(s) 75, 163
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 61, 377
MluCI AATT 3 cut(s) 12, 50, 438
MlyI GAGTC 2 cut(s) 198, 506
MmeI TCCRAC 3 cut(s) 300, 383, 440
Mph1103I ATGCAT 1 cut(s) 250
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 500
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 1 cut(s) 460
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 460
NdeII GATC 1 cut(s) 385
NheI GCTAGC 1 cut(s) 112
NlaIII CATG 5 cut(s) 26, 265, 318, 478, 502
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 73, 298
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 2 cut(s) 26, 318
PciI ACATGT 1 cut(s) 22
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 2 cut(s) 163, 166
PleI GAGTC 2 cut(s) 198, 506
PpsI GAGTC 2 cut(s) 198, 506
PscI ACATGT 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 58
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 2 cut(s) 82, 473
RsaNI GTAC 2 cut(s) 81, 472
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 2 cut(s) 162, 165
Sau3AI GATC 1 cut(s) 385
SchI GAGTC 2 cut(s) 198, 506
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 2 cut(s) 61, 377
SetI ASST 5 cut(s) 114, 365, 372, 492, 524
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 500
SpeI ACTAGT 1 cut(s) 77
Sse9I AATT 3 cut(s) 12, 50, 438
SsiI CCGC 1 cut(s) 146
SspMI CTAG 3 cut(s) 78, 113, 465
StyD4I CCNGG 1 cut(s) 458
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 365
TasI AATT 3 cut(s) 12, 50, 438
TatI WGTACW 2 cut(s) 80, 471
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 2 cut(s) 244, 356
TseFI GTSAC 2 cut(s) 73, 298
TseI GCWGC 2 cut(s) 161, 164
Tsp45I GTSAC 2 cut(s) 73, 298
TspDTI ATGAA 6 cut(s) 17, 63, 259, 272, 491, 500
TspRI CASTG 2 cut(s) 244, 356
XceI RCATGY 2 cut(s) 26, 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XspI CTAG 3 cut(s) 78, 113, 465
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.