Rmu_sc0017770.1_g000005

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017770.1
OG0012747
Physical Location & Seq
Reverse (-)
13340 .. 15104
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017770.1_g000005.1.cds

Sequence Viewer

Length: 1095 bp
atgtctcctcctagagtttgttccggtgccaccatggctgttgccctagtgctaatggtggcaatgagcccggtgattctcgcacctcatcaagctgaggcagctcgcgctttctttgtgttcggagactcccttgtggataacggcaacaacaattacttggccaccaccgcgcgtgccgattttcctccctacggcattgatttccctacccaccgccccaccggccgcttctccaacggcttaaacctccctgatctcatcagcgagcaaataggatcggagctagtcttgccatatttgagcagtgagctcaccggacaaaagctgcttattggtgctaactttgcttctgctggaattggaatcctcaatgatactggtgttcaattttttgagatgttccagcaataccagcaacgagtgagtgccctaatcggagtagcgcaagccaagaggctagtaaacgacgctcttgtcctcataactctcggcggcaatgacttcgtcaacaactacttcttggtacccttctctgcaagatcacgccagtactcaatccctcagtactcccagtacctcatctccgagtaccggaaaattctcctgagactatttgaattaggagggagaagggtgctggtgaccggtacaggcccattaggctgtgttcccgccgctatggcccagaggagtaggaatggtgaatgtgcaccagagctccagagggcagcagccatcttcaatcccctgcttgtccagatggttcaggggctcaacgctcagcttggtactgacgctttcattgctgctaatgcgtttgcaaatgtggagttcctcagtaacccccggaaatatggctttgttacgtcaaaggtggcatgttgcgggcaaggaccgtacaatgggttgggactatgtaccccattgtcgaatctgtgctccaaccgggatgtgtatgcgttttgggatcctttccatccatctgagcgggcgaaccggctgattgtgcagacgatcatggccgggtctagcaagtacatgagccctatgaacctaagcaccatcatggccatggactccgggaataactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

39.45

Weight (kDa)

8.45

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
Acc65I GGTACC 1 cut(s) 526
AccB1I GGYRCC 2 cut(s) 26, 526
AccBSI CCGCTC 1 cut(s) 991
AccII CGCG 3 cut(s) 108, 173, 175
AciI CCGC 8 cut(s) 171, 217, 229, 495, 675, 678, 888, 991
AclWI GGATC 3 cut(s) 286, 965, 978
AcoI YGGCCR 4 cut(s) 162, 226, 1023, 1071
AcsI RAATTY 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 194, 594, 905
AgeI ACCGGT 1 cut(s) 647
AgsI TTSAA 3 cut(s) 389, 620, 745
AluBI AGCT 7 cut(s) 95, 104, 286, 313, 328, 721, 787
AluI AGCT 7 cut(s) 95, 104, 286, 313, 328, 721, 787
Alw21I GWGCWC 4 cut(s) 315, 715, 723, 944
Alw26I GTCTC 3 cut(s) 9, 120, 604
Alw44I GTGCAC 1 cut(s) 711
AlwI GGATC 3 cut(s) 286, 965, 978
AoxI GGCC 6 cut(s) 162, 226, 655, 684, 1023, 1071
ApaLI GTGCAC 1 cut(s) 711
ApeKI GCWGC 5 cut(s) 101, 328, 731, 734, 809
ApoI RAATTY 1 cut(s) 600
AsiGI ACCGGT 1 cut(s) 647
Asp718I GGTACC 1 cut(s) 526
AspLEI GCGC 3 cut(s) 110, 175, 448
AspS9I GGNCC 3 cut(s) 656, 685, 896
AsuC2I CCSGG 5 cut(s) 71, 850, 950, 1027, 1084
AsuHPI GGTGA 4 cut(s) 85, 307, 655, 716
AvaII GGWCC 1 cut(s) 896
BaeGI GKGCMC 2 cut(s) 433, 715
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BalI TGGCCA 2 cut(s) 164, 1073
BamHI GGATCC 1 cut(s) 970
BanI GGYRCC 2 cut(s) 26, 526
BanII GRGCYC 5 cut(s) 71, 315, 723, 777, 1049
Bbv12I GWGCWC 4 cut(s) 315, 715, 723, 944
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 5 cut(s) 113, 315, 743, 746, 796
BccI CCATC 5 cut(s) 746, 757, 987, 991, 1073
BceAI ACGGC 3 cut(s) 160, 211, 256
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BcnI CCSGG 5 cut(s) 71, 850, 950, 1027, 1084
BcoDI GTCTC 3 cut(s) 9, 120, 604
BfaI CTAG 6 cut(s) 12, 47, 287, 461, 1032, 1093
BglI GCCNNNNNGGC 4 cut(s) 35, 225, 663, 683
BisI GCNGC 8 cut(s) 102, 229, 329, 496, 678, 732, 735, 810
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 8 cut(s) 103, 230, 330, 497, 679, 733, 736, 811
BmcAI AGTACT 2 cut(s) 554, 569
Bme1390I CCNGG 5 cut(s) 71, 850, 950, 1027, 1084
Bme18I GGWCC 1 cut(s) 896
BmgT120I GGNCC 3 cut(s) 656, 685, 896
BmiI GGNNCC 3 cut(s) 28, 528, 972
BmrFI CCNGG 5 cut(s) 71, 850, 950, 1027, 1084
BmrI ACTGGG 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 707
Bpu10I CCTNAGC 2 cut(s) 96, 1058
Bpu1102I GCTNAGC 1 cut(s) 783
BpuMI CCSGG 5 cut(s) 71, 850, 950, 1027, 1084
BsaJI CCNNGG 3 cut(s) 33, 848, 1074
BsaWI WCCGGW 4 cut(s) 23, 317, 594, 647
BsaXI ACNNNNNCTCC 4 cut(s) 569, 599, 705, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 594, 905
Bse118I RCCGGY 3 cut(s) 224, 647, 999
Bse1I ACTGG 3 cut(s) 385, 550, 574
Bse3DI GCAATG 3 cut(s) 69, 505, 804
BseDI CCNNGG 3 cut(s) 33, 848, 1074
BseGI GGATG 2 cut(s) 958, 979
BseLI CCNNNNNNNGG 3 cut(s) 194, 594, 905
BseMI GCAATG 3 cut(s) 69, 505, 804
BseMII CTCAG 6 cut(s) 87, 578, 599, 797, 853, 978
BseNI ACTGG 3 cut(s) 385, 550, 574
BseRI GAGGAG 1 cut(s) 706
BseSI GKGCMC 2 cut(s) 433, 715
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 5 cut(s) 113, 315, 743, 746, 796
BsgI GTGCAG 1 cut(s) 1031
Bsh1236I CGCG 3 cut(s) 108, 173, 175
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 6 cut(s) 164, 228, 657, 686, 1025, 1073
BshNI GGYRCC 2 cut(s) 26, 526
BshTI ACCGGT 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 4 cut(s) 315, 715, 723, 944
BslFI GGGAC 1 cut(s) 927
BslI CCNNNNNNNGG 3 cut(s) 194, 594, 905
BsmAI GTCTC 3 cut(s) 9, 120, 604
BsmFI GGGAC 1 cut(s) 927
BsnI GGCC 6 cut(s) 164, 228, 657, 686, 1025, 1073
Bsp1286I GDGCHC 8 cut(s) 71, 315, 433, 715, 723, 777, 944, 1049
Bsp143I GATC 5 cut(s) 256, 278, 542, 970, 1017
Bsp1720I GCTNAGC 1 cut(s) 783
Bsp19I CCATGG 2 cut(s) 33, 1074
BspACI CCGC 8 cut(s) 171, 217, 229, 495, 675, 678, 888, 991
BspANI GGCC 6 cut(s) 164, 228, 657, 686, 1025, 1073
BspCNI CTCAG 6 cut(s) 88, 577, 600, 796, 852, 979
BspFNI CGCG 3 cut(s) 108, 173, 175
BspLI GGNNCC 3 cut(s) 28, 528, 972
BspPI GGATC 3 cut(s) 286, 965, 978
BspT107I GGYRCC 2 cut(s) 26, 526
BsrBI CCGCTC 1 cut(s) 991
BsrDI GCAATG 3 cut(s) 69, 505, 804
BsrFI RCCGGY 3 cut(s) 224, 647, 999
BsrI ACTGG 3 cut(s) 385, 550, 574
BssAI RCCGGY 3 cut(s) 224, 647, 999
BssECI CCNNGG 3 cut(s) 33, 848, 1074
BssMI GATC 5 cut(s) 256, 278, 542, 970, 1017
BssT1I CCWWGG 2 cut(s) 33, 1074
Bst4CI ACNGT 1 cut(s) 900
BstC8I GCNNGC 6 cut(s) 106, 177, 269, 450, 890, 993
BstDEI CTNAG 7 cut(s) 96, 564, 608, 783, 839, 987, 1058
BstDSI CCRYGG 2 cut(s) 33, 1074
BstEII GGTNACC 1 cut(s) 643
BstF5I GGATG 2 cut(s) 958, 979
BstFNI CGCG 3 cut(s) 108, 173, 175
BstHHI GCGC 3 cut(s) 110, 175, 448
BstKTI GATC 5 cut(s) 259, 281, 545, 973, 1020
BstMAI GTCTC 3 cut(s) 9, 120, 604
BstMBI GATC 5 cut(s) 256, 278, 542, 970, 1017
BstMCI CGRYCG 1 cut(s) 229
BstNSI RCATGY 1 cut(s) 885
BstPI GGTNACC 1 cut(s) 643
BstSCI CCNGG 5 cut(s) 69, 848, 948, 1025, 1082
BstSLI GKGCMC 2 cut(s) 433, 715
BstUI CGCG 3 cut(s) 108, 173, 175
BstV1I GCAGC 5 cut(s) 113, 315, 743, 746, 796
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 6 cut(s) 164, 228, 657, 686, 1025, 1073
BtgI CCRYGG 2 cut(s) 33, 1074
BtsCI GGATG 2 cut(s) 958, 979
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 6 cut(s) 106, 177, 269, 450, 890, 993
CfoI GCGC 3 cut(s) 110, 175, 448
Cfr10I RCCGGY 3 cut(s) 224, 647, 999
Cfr13I GGNCC 3 cut(s) 656, 685, 896
CseI GACGC 2 cut(s) 479, 806
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 6 cut(s) 34, 882, 1021, 1042, 1069, 1075
DdeI CTNAG 7 cut(s) 96, 564, 608, 783, 839, 987, 1058
DpnI GATC 5 cut(s) 258, 280, 544, 972, 1019
DpnII GATC 5 cut(s) 256, 278, 542, 970, 1017
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
EaeI YGGCCR 4 cut(s) 162, 226, 1023, 1071
EagI CGGCCG 1 cut(s) 226
Ecl136II GAGCTC 2 cut(s) 313, 721
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 33, 1074
Eco24I GRGCYC 5 cut(s) 71, 315, 723, 777, 1049
Eco47I GGWCC 1 cut(s) 896
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 2 cut(s) 313, 721
Eco91I GGTNACC 1 cut(s) 643
EcoICRI GAGCTC 2 cut(s) 313, 721
EcoO65I GGTNACC 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 33, 1074
EcoT38I GRGCYC 5 cut(s) 71, 315, 723, 777, 1049
ErhI CCWWGG 2 cut(s) 33, 1074
FaeI CATG 6 cut(s) 37, 885, 1024, 1045, 1072, 1078
FaqI GGGAC 1 cut(s) 927
FatI CATG 6 cut(s) 33, 881, 1020, 1041, 1068, 1074
FauI CCCGC 3 cut(s) 682, 881, 984
Fnu4HI GCNGC 8 cut(s) 102, 229, 329, 496, 678, 732, 735, 810
FokI GGATG 2 cut(s) 965, 966
FriOI GRGCYC 5 cut(s) 71, 315, 723, 777, 1049
Fsp4HI GCNGC 8 cut(s) 102, 229, 329, 496, 678, 732, 735, 810
FspBI CTAG 6 cut(s) 12, 47, 287, 461, 1032, 1093
GlaI GCGC 3 cut(s) 109, 174, 447
GluI GCNGC 8 cut(s) 102, 229, 329, 496, 678, 732, 735, 810
GsuI CTGGAG 1 cut(s) 707
HaeIII GGCC 6 cut(s) 164, 228, 657, 686, 1025, 1073
HgaI GACGC 2 cut(s) 479, 806
HhaI GCGC 3 cut(s) 110, 175, 448
Hin1II CATG 6 cut(s) 37, 885, 1024, 1045, 1072, 1078
Hin6I GCGC 3 cut(s) 108, 173, 446
HinP1I GCGC 3 cut(s) 108, 173, 446
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 5 cut(s) 76, 128, 366, 934, 1079
HphI GGTGA 4 cut(s) 85, 307, 655, 716
Hpy166II GTNNAC 3 cut(s) 466, 511, 713
Hpy188I TCNGA 5 cut(s) 125, 283, 440, 589, 988
Hpy188III TCNNGA 3 cut(s) 607, 724, 760
Hpy8I GTNNAC 3 cut(s) 466, 511, 713
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 2 cut(s) 541, 627
HpyCH4III ACNGT 1 cut(s) 900
HpyCH4IV ACGT 1 cut(s) 869
HpyCH4V TGCA 4 cut(s) 539, 713, 824, 1012
HpyF3I CTNAG 7 cut(s) 96, 564, 608, 783, 839, 987, 1058
HpySE526I ACGT 1 cut(s) 869
Hsp92II CATG 6 cut(s) 37, 885, 1024, 1045, 1072, 1078
HspAI GCGC 3 cut(s) 108, 173, 446
KpnI GGTACC 1 cut(s) 530
Kzo9I GATC 5 cut(s) 256, 278, 542, 970, 1017
LmnI GCTCC 3 cut(s) 283, 726, 947
Lsp1109I GCAGC 5 cut(s) 113, 315, 743, 746, 796
MaeI CTAG 6 cut(s) 12, 47, 287, 461, 1032, 1093
MaeII ACGT 1 cut(s) 869
MaeIII GTNAC 3 cut(s) 643, 842, 865
MalI GATC 5 cut(s) 258, 280, 544, 972, 1019
MbiI CCGCTC 1 cut(s) 991
MboI GATC 5 cut(s) 256, 278, 542, 970, 1017
MboII GAAGA 1 cut(s) 733
MflI RGATCY 1 cut(s) 970
MhlI GDGCHC 8 cut(s) 71, 315, 433, 715, 723, 777, 944, 1049
MlsI TGGCCA 2 cut(s) 164, 1073
MluCI AATT 5 cut(s) 154, 360, 389, 600, 620
MluNI TGGCCA 2 cut(s) 164, 1073
MlyI GAGTC 2 cut(s) 122, 1073
MmeI TCCRAC 2 cut(s) 261, 969
Mox20I TGGCCA 2 cut(s) 164, 1073
MscI TGGCCA 2 cut(s) 164, 1073
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 2 cut(s) 1067, 1073
Msp20I TGGCCA 2 cut(s) 164, 1073
MspR9I CCNGG 5 cut(s) 71, 850, 950, 1027, 1084
MvnI CGCG 3 cut(s) 108, 173, 175
NciI CCSGG 5 cut(s) 71, 850, 950, 1027, 1084
NcoI CCATGG 2 cut(s) 33, 1074
NdeII GATC 5 cut(s) 256, 278, 542, 970, 1017
NlaIII CATG 6 cut(s) 37, 885, 1024, 1045, 1072, 1078
NlaIV GGNNCC 3 cut(s) 28, 528, 972
NmeAIII GCCGAG 1 cut(s) 471
NmuCI GTSAC 1 cut(s) 643
NspI RCATGY 1 cut(s) 885
PfeI GAWTC 3 cut(s) 76, 366, 934
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 1082
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 8 cut(s) 103, 230, 330, 497, 679, 733, 736, 811
PleI GAGTC 2 cut(s) 122, 1073
PpsI GAGTC 2 cut(s) 122, 1073
Psp124BI GAGCTC 2 cut(s) 315, 723
PspEI GGTNACC 1 cut(s) 643
PspN4I GGNNCC 3 cut(s) 28, 528, 972
PspPI GGNCC 3 cut(s) 656, 685, 896
PsuI RGATCY 1 cut(s) 970
PsyI GACNNNGTC 1 cut(s) 506
RseI CAYNNNNRTG 2 cut(s) 1067, 1073
SacI GAGCTC 2 cut(s) 315, 723
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 8 cut(s) 102, 229, 329, 496, 678, 732, 735, 810
Sau3AI GATC 5 cut(s) 256, 278, 542, 970, 1017
Sau96I GGNCC 3 cut(s) 656, 685, 896
ScaI AGTACT 2 cut(s) 554, 569
SchI GAGTC 2 cut(s) 122, 1073
ScrFI CCNGG 5 cut(s) 71, 850, 950, 1027, 1084
SduI GDGCHC 8 cut(s) 71, 315, 433, 715, 723, 777, 944, 1049
SinI GGWCC 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 1067, 1073
Sse9I AATT 5 cut(s) 154, 360, 389, 600, 620
SsiI CCGC 8 cut(s) 171, 217, 229, 495, 675, 678, 888, 991
SspMI CTAG 6 cut(s) 12, 47, 287, 461, 1032, 1093
SstI GAGCTC 2 cut(s) 315, 723
StyD4I CCNGG 5 cut(s) 69, 848, 948, 1025, 1082
StyI CCWWGG 2 cut(s) 33, 1074
TaaI ACNGT 1 cut(s) 900
TaiI ACGT 1 cut(s) 872
TaqI TCGA 1 cut(s) 932
TasI AATT 5 cut(s) 154, 360, 389, 600, 620
TatI WGTACW 3 cut(s) 552, 567, 1038
TauI GCSGC 3 cut(s) 231, 498, 680
TfiI GAWTC 3 cut(s) 76, 366, 934
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 5 cut(s) 101, 328, 731, 734, 809
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 2 cut(s) 793, 1067
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 506
VneI GTGCAC 1 cut(s) 711
VpaK11BI GGWCC 1 cut(s) 896
XapI RAATTY 1 cut(s) 600
XceI RCATGY 1 cut(s) 885
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XspI CTAG 6 cut(s) 12, 47, 287, 461, 1032, 1093
ZrmI AGTACT 2 cut(s) 554, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.