Rh6BG288400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012747
Physical Location & Seq
Reverse (-)
51355726 .. 51357644
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG288400.1

Sequence Viewer

Length: 1122 bp
ATGTCTCCTCGTAGGGTTTGTTCCGGTGCCACCATGGCTGTTGCCCTAGTGCTAATGGTGGCAATGAGCACGGTGATTCTCGCACCTCATCAAGCTGAGGCAGCTCGCGCTTTCTTCGTTTTCGGAGACTCCCTTGTGGATAACGGCAACAACAATTACTTGGCCACCACCGCGCGTGCCGATTTTCCTCCCTACGGCATTGATTTCCCTACCCACCGCCCCACCGGCCGCTTCTCCAACGGCTTAAACCTCCCTGATCTCATCAGCGAGCAAATAGGATCGGAGCTAGTCTTGCCATATTTGAGCAGTGAGCTCACCGGACAAAAGCTGCTTATTGGTGCTAACTTTGCTTCTGCTGGAATTGGAATCCTCAATGATACTGGTGTTCAATTTATCAACATAATAAGGATCTTTAGGCAGTTTGAGATGTTCCAGCAATATCAGCAACGAGTGAGTGCCCTAATCGGAGCAGCGCAAGCCAAGAGGCTGGTAAACGACGCTCTTGTCCTCATAACTCTCGGCGGCAATGACTTCGTCAACAACTACTTCTTGGTACCCTTCTCTGCAAGATCACTCCAGTACTCAATCCCTCAGTACTCCCAGTACCTCATCTCCGAGTACCGGAAAATTCTCCTGAGACTATTTGAATTAGGAGGGAGAAGGATGCTGGTGACCGGTACAGGCCCATTAGGCTGTGTTCCCGCCGCTATGGCCCAGAGGAGTAGGAATGGTGAATGTGCACCAGAGCTCCAGAGGGCAGCAGCCATCTTCAATCCCCTGCTTGTCCAGATGGTTCAGGGGCTCAACGCTCAGCTTGGTACTGACGCTTTCATTGCTGCTAATGCGTTTGCAAATGTGGAGTTCCTCAGTAACCCCCGGAAATATGGCTTTGTTACGTCAAAGGTGGCATGTTGCGGGCAAGGACCGTACAATGGGTTGGGACTATGTACCCCATTGTCGAATCTGTGCTCCAACCGGGATGTGTATGCGTTTTGGGATCCTTTCCATCCATCTGAGCGGGCGAACCGGCTGATTGTGCAGACGATCATGGCCGGGTCTAGCAAGTACATGAGCCCTATGAACCTAAGCACCATCATGGCCATGGACTCCGGGAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

40.63

Weight (kDa)

8.83

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 347 2.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 503
Acc65I GGTACC 1 cut(s) 553
AccB1I GGYRCC 2 cut(s) 26, 553
AccBSI CCGCTC 1 cut(s) 1018
AccII CGCG 3 cut(s) 108, 173, 175
AciI CCGC 8 cut(s) 171, 217, 229, 522, 702, 705, 915, 1018
AclWI GGATC 4 cut(s) 286, 416, 992, 1005
AcoI YGGCCR 4 cut(s) 162, 226, 1050, 1098
AcsI RAATTY 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 194, 621, 932
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 3 cut(s) 389, 647, 772
AluBI AGCT 7 cut(s) 95, 104, 286, 313, 328, 748, 814
AluI AGCT 7 cut(s) 95, 104, 286, 313, 328, 748, 814
Alw21I GWGCWC 5 cut(s) 71, 315, 742, 750, 971
Alw26I GTCTC 3 cut(s) 9, 120, 631
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 4 cut(s) 286, 416, 992, 1005
AoxI GGCC 6 cut(s) 162, 226, 682, 711, 1050, 1098
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 6 cut(s) 101, 328, 470, 758, 761, 836
ApoI RAATTY 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 674
Asp718I GGTACC 1 cut(s) 553
AspLEI GCGC 3 cut(s) 110, 175, 475
AspS9I GGNCC 3 cut(s) 683, 712, 923
AsuC2I CCSGG 4 cut(s) 877, 977, 1054, 1111
AsuHPI GGTGA 4 cut(s) 85, 307, 682, 743
AvaII GGWCC 1 cut(s) 923
BaeGI GKGCMC 2 cut(s) 460, 742
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BalI TGGCCA 2 cut(s) 164, 1100
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 2 cut(s) 26, 553
BanII GRGCYC 4 cut(s) 315, 750, 804, 1076
Bbv12I GWGCWC 5 cut(s) 71, 315, 742, 750, 971
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 6 cut(s) 113, 315, 482, 770, 773, 823
BccI CCATC 5 cut(s) 773, 784, 1014, 1018, 1100
BceAI ACGGC 3 cut(s) 160, 211, 256
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BcnI CCSGG 4 cut(s) 877, 977, 1054, 1111
BcoDI GTCTC 3 cut(s) 9, 120, 631
BfaI CTAG 4 cut(s) 47, 287, 1059, 1120
BglI GCCNNNNNGGC 4 cut(s) 35, 225, 690, 710
BisI GCNGC 9 cut(s) 102, 229, 329, 471, 523, 705, 759, 762, 837
BlpI GCTNAGC 1 cut(s) 810
BlsI GCNGC 9 cut(s) 103, 230, 330, 472, 524, 706, 760, 763, 838
BmcAI AGTACT 2 cut(s) 581, 596
Bme1390I CCNGG 4 cut(s) 877, 977, 1054, 1111
Bme18I GGWCC 1 cut(s) 923
BmgT120I GGNCC 3 cut(s) 683, 712, 923
BmiI GGNNCC 3 cut(s) 28, 555, 999
BmrFI CCNGG 4 cut(s) 877, 977, 1054, 1111
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 1 cut(s) 654
BmuI ACTGGG 1 cut(s) 595
BpmI CTGGAG 2 cut(s) 560, 734
Bpu10I CCTNAGC 2 cut(s) 96, 1085
Bpu1102I GCTNAGC 1 cut(s) 810
BpuMI CCSGG 4 cut(s) 877, 977, 1054, 1111
BsaJI CCNNGG 3 cut(s) 33, 875, 1101
BsaWI WCCGGW 4 cut(s) 23, 317, 621, 674
BsaXI ACNNNNNCTCC 4 cut(s) 596, 626, 732, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 621, 932
Bse118I RCCGGY 3 cut(s) 224, 674, 1026
Bse1I ACTGG 3 cut(s) 385, 577, 601
Bse3DI GCAATG 3 cut(s) 69, 532, 831
BseDI CCNNGG 3 cut(s) 33, 875, 1101
BseGI GGATG 3 cut(s) 669, 985, 1006
BseLI CCNNNNNNNGG 3 cut(s) 194, 621, 932
BseMI GCAATG 3 cut(s) 69, 532, 831
BseMII CTCAG 6 cut(s) 87, 605, 626, 824, 880, 1005
BseNI ACTGG 3 cut(s) 385, 577, 601
BseRI GAGGAG 1 cut(s) 733
BseSI GKGCMC 2 cut(s) 460, 742
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 6 cut(s) 113, 315, 482, 770, 773, 823
BsgI GTGCAG 1 cut(s) 1058
Bsh1236I CGCG 3 cut(s) 108, 173, 175
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 6 cut(s) 164, 228, 684, 713, 1052, 1100
BshNI GGYRCC 2 cut(s) 26, 553
BshTI ACCGGT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 5 cut(s) 71, 315, 742, 750, 971
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 3 cut(s) 194, 621, 932
BsmAI GTCTC 3 cut(s) 9, 120, 631
BsmFI GGGAC 1 cut(s) 954
BsnI GGCC 6 cut(s) 164, 228, 684, 713, 1052, 1100
Bsp1286I GDGCHC 8 cut(s) 71, 315, 460, 742, 750, 804, 971, 1076
Bsp143I GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
Bsp1720I GCTNAGC 1 cut(s) 810
Bsp19I CCATGG 2 cut(s) 33, 1101
BspACI CCGC 8 cut(s) 171, 217, 229, 522, 702, 705, 915, 1018
BspANI GGCC 6 cut(s) 164, 228, 684, 713, 1052, 1100
BspCNI CTCAG 6 cut(s) 88, 604, 627, 823, 879, 1006
BspFNI CGCG 3 cut(s) 108, 173, 175
BspLI GGNNCC 3 cut(s) 28, 555, 999
BspPI GGATC 4 cut(s) 286, 416, 992, 1005
BspT107I GGYRCC 2 cut(s) 26, 553
BsrBI CCGCTC 1 cut(s) 1018
BsrDI GCAATG 3 cut(s) 69, 532, 831
BsrFI RCCGGY 3 cut(s) 224, 674, 1026
BsrI ACTGG 3 cut(s) 385, 577, 601
BssAI RCCGGY 3 cut(s) 224, 674, 1026
BssECI CCNNGG 3 cut(s) 33, 875, 1101
BssMI GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
BssT1I CCWWGG 2 cut(s) 33, 1101
Bst4CI ACNGT 2 cut(s) 73, 927
BstC8I GCNNGC 6 cut(s) 106, 177, 269, 477, 917, 1020
BstDEI CTNAG 7 cut(s) 96, 591, 635, 810, 866, 1014, 1085
BstDSI CCRYGG 2 cut(s) 33, 1101
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 3 cut(s) 669, 985, 1006
BstFNI CGCG 3 cut(s) 108, 173, 175
BstHHI GCGC 3 cut(s) 110, 175, 475
BstKTI GATC 6 cut(s) 259, 281, 411, 572, 1000, 1047
BstMAI GTCTC 3 cut(s) 9, 120, 631
BstMBI GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
BstMCI CGRYCG 1 cut(s) 229
BstNSI RCATGY 1 cut(s) 912
BstPI GGTNACC 1 cut(s) 670
BstSCI CCNGG 4 cut(s) 875, 975, 1052, 1109
BstSLI GKGCMC 2 cut(s) 460, 742
BstUI CGCG 3 cut(s) 108, 173, 175
BstV1I GCAGC 6 cut(s) 113, 315, 482, 770, 773, 823
BstX2I RGATCY 2 cut(s) 408, 997
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 2 cut(s) 408, 997
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 6 cut(s) 164, 228, 684, 713, 1052, 1100
BtgI CCRYGG 2 cut(s) 33, 1101
BtsCI GGATG 3 cut(s) 669, 985, 1006
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 6 cut(s) 106, 177, 269, 477, 917, 1020
CfoI GCGC 3 cut(s) 110, 175, 475
Cfr10I RCCGGY 3 cut(s) 224, 674, 1026
Cfr13I GGNCC 3 cut(s) 683, 712, 923
CseI GACGC 2 cut(s) 506, 833
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 6 cut(s) 34, 909, 1048, 1069, 1096, 1102
DdeI CTNAG 7 cut(s) 96, 591, 635, 810, 866, 1014, 1085
DpnI GATC 6 cut(s) 258, 280, 410, 571, 999, 1046
DpnII GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 4 cut(s) 162, 226, 1050, 1098
EagI CGGCCG 1 cut(s) 226
Ecl136II GAGCTC 2 cut(s) 313, 748
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 33, 1101
Eco24I GRGCYC 4 cut(s) 315, 750, 804, 1076
Eco47I GGWCC 1 cut(s) 923
Eco52I CGGCCG 1 cut(s) 226
Eco53kI GAGCTC 2 cut(s) 313, 748
Eco91I GGTNACC 1 cut(s) 670
EcoICRI GAGCTC 2 cut(s) 313, 748
EcoO65I GGTNACC 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 33, 1101
EcoT38I GRGCYC 4 cut(s) 315, 750, 804, 1076
ErhI CCWWGG 2 cut(s) 33, 1101
FaeI CATG 6 cut(s) 37, 912, 1051, 1072, 1099, 1105
FaqI GGGAC 1 cut(s) 954
FatI CATG 6 cut(s) 33, 908, 1047, 1068, 1095, 1101
FauI CCCGC 3 cut(s) 709, 908, 1011
Fnu4HI GCNGC 9 cut(s) 102, 229, 329, 471, 523, 705, 759, 762, 837
FokI GGATG 3 cut(s) 676, 992, 993
FriOI GRGCYC 4 cut(s) 315, 750, 804, 1076
Fsp4HI GCNGC 9 cut(s) 102, 229, 329, 471, 523, 705, 759, 762, 837
FspBI CTAG 4 cut(s) 47, 287, 1059, 1120
GlaI GCGC 3 cut(s) 109, 174, 474
GluI GCNGC 9 cut(s) 102, 229, 329, 471, 523, 705, 759, 762, 837
GsuI CTGGAG 2 cut(s) 560, 734
HaeIII GGCC 6 cut(s) 164, 228, 684, 713, 1052, 1100
HgaI GACGC 2 cut(s) 506, 833
HhaI GCGC 3 cut(s) 110, 175, 475
Hin1II CATG 6 cut(s) 37, 912, 1051, 1072, 1099, 1105
Hin6I GCGC 3 cut(s) 108, 173, 473
HinP1I GCGC 3 cut(s) 108, 173, 473
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 5 cut(s) 76, 128, 366, 961, 1106
HphI GGTGA 4 cut(s) 85, 307, 682, 743
Hpy166II GTNNAC 3 cut(s) 493, 538, 740
Hpy188I TCNGA 5 cut(s) 125, 283, 467, 616, 1015
Hpy188III TCNNGA 3 cut(s) 634, 751, 787
Hpy8I GTNNAC 3 cut(s) 493, 538, 740
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 568, 654
HpyCH4III ACNGT 2 cut(s) 73, 927
HpyCH4IV ACGT 1 cut(s) 896
HpyCH4V TGCA 4 cut(s) 566, 740, 851, 1039
HpyF3I CTNAG 7 cut(s) 96, 591, 635, 810, 866, 1014, 1085
HpySE526I ACGT 1 cut(s) 896
Hsp92II CATG 6 cut(s) 37, 912, 1051, 1072, 1099, 1105
HspAI GCGC 3 cut(s) 108, 173, 473
KpnI GGTACC 1 cut(s) 557
Kzo9I GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
LmnI GCTCC 4 cut(s) 283, 467, 753, 974
Lsp1109I GCAGC 6 cut(s) 113, 315, 482, 770, 773, 823
LweI GCATC 1 cut(s) 654
MaeI CTAG 4 cut(s) 47, 287, 1059, 1120
MaeII ACGT 1 cut(s) 896
MaeIII GTNAC 3 cut(s) 670, 869, 892
MalI GATC 6 cut(s) 258, 280, 410, 571, 999, 1046
MbiI CCGCTC 1 cut(s) 1018
MboI GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
MboII GAAGA 2 cut(s) 106, 760
MflI RGATCY 2 cut(s) 408, 997
MhlI GDGCHC 8 cut(s) 71, 315, 460, 742, 750, 804, 971, 1076
MlsI TGGCCA 2 cut(s) 164, 1100
MluCI AATT 5 cut(s) 154, 360, 389, 627, 647
MluNI TGGCCA 2 cut(s) 164, 1100
MlyI GAGTC 2 cut(s) 122, 1100
MmeI TCCRAC 2 cut(s) 261, 996
Mox20I TGGCCA 2 cut(s) 164, 1100
MscI TGGCCA 2 cut(s) 164, 1100
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 2 cut(s) 1094, 1100
Msp20I TGGCCA 2 cut(s) 164, 1100
MspR9I CCNGG 4 cut(s) 877, 977, 1054, 1111
MvnI CGCG 3 cut(s) 108, 173, 175
NciI CCSGG 4 cut(s) 877, 977, 1054, 1111
NcoI CCATGG 2 cut(s) 33, 1101
NdeII GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
NlaIII CATG 6 cut(s) 37, 912, 1051, 1072, 1099, 1105
NlaIV GGNNCC 3 cut(s) 28, 555, 999
NmeAIII GCCGAG 1 cut(s) 498
NmuCI GTSAC 1 cut(s) 670
NspI RCATGY 1 cut(s) 912
PfeI GAWTC 3 cut(s) 76, 366, 961
PflFI GACNNNGTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 1109
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 9 cut(s) 103, 230, 330, 472, 524, 706, 760, 763, 838
PleI GAGTC 2 cut(s) 122, 1100
PpsI GAGTC 2 cut(s) 122, 1100
Psp124BI GAGCTC 2 cut(s) 315, 750
PspEI GGTNACC 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 28, 555, 999
PspPI GGNCC 3 cut(s) 683, 712, 923
PsrI GAACNNNNNNTAC 1 cut(s) 36
PsuI RGATCY 2 cut(s) 408, 997
PsyI GACNNNGTC 1 cut(s) 533
RseI CAYNNNNRTG 2 cut(s) 1094, 1100
SacI GAGCTC 2 cut(s) 315, 750
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 9 cut(s) 102, 229, 329, 471, 523, 705, 759, 762, 837
Sau3AI GATC 6 cut(s) 256, 278, 408, 569, 997, 1044
Sau96I GGNCC 3 cut(s) 683, 712, 923
ScaI AGTACT 2 cut(s) 581, 596
SchI GAGTC 2 cut(s) 122, 1100
ScrFI CCNGG 4 cut(s) 877, 977, 1054, 1111
SduI GDGCHC 8 cut(s) 71, 315, 460, 742, 750, 804, 971, 1076
SfaNI GCATC 1 cut(s) 654
SinI GGWCC 1 cut(s) 923
SmiMI CAYNNNNRTG 2 cut(s) 1094, 1100
Sse9I AATT 5 cut(s) 154, 360, 389, 627, 647
SsiI CCGC 8 cut(s) 171, 217, 229, 522, 702, 705, 915, 1018
SspMI CTAG 4 cut(s) 47, 287, 1059, 1120
SstI GAGCTC 2 cut(s) 315, 750
StyD4I CCNGG 4 cut(s) 875, 975, 1052, 1109
StyI CCWWGG 2 cut(s) 33, 1101
TaaI ACNGT 2 cut(s) 73, 927
TaiI ACGT 1 cut(s) 899
TaqI TCGA 1 cut(s) 959
TasI AATT 5 cut(s) 154, 360, 389, 627, 647
TatI WGTACW 3 cut(s) 579, 594, 1065
TauI GCSGC 3 cut(s) 231, 525, 707
TfiI GAWTC 3 cut(s) 76, 366, 961
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 6 cut(s) 101, 328, 470, 758, 761, 836
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 2 cut(s) 820, 1094
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 533
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 1 cut(s) 923
XapI RAATTY 1 cut(s) 627
XceI RCATGY 1 cut(s) 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XspI CTAG 4 cut(s) 47, 287, 1059, 1120
ZrmI AGTACT 2 cut(s) 581, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.