AT4G30030

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017135
Physical Location & Seq
Reverse (-)
14682210 .. 14683484
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30030.1

Sequence Viewer

Length: 1275 bp
ATGGCCATCACTCTACTACCATTACGTATTCGTGCCTCCCTTCTTATCATTATATTCGCTCTCACTTGTTCAAAGGAATGCACCTCGCATTCCCGACTTACGCTAAGAACCAAAACCCAAGAATCATCGAAAATCAAAATCGGTTACTTGCATTCCAAGTCAACCCCCGCTTCTCGCCTAGATAACTTGTGGACCGTCTCACATGTCACTCCTATTCCGAATCCAGCAGCATTCCTTGCAAATATTTCTATCGGCAATCCACCTGTCCCGCAACTCTTACTCATAGATACAGGTAGTGACCTAACTTGGATCCATTGTCTTCCCTGCAAATGTTACCCTCAAACAATCCCTTTTTTCCACCCTTCAAGATCTTCCACTTACCGAAACGCTTCTTGTGTATCCGCACCACACGCCATGCCCCAAATCTTTCGCGACGAAAAGACTGGAAACTGCCAATATCACCTACGCTACCGCGACTTTTCTAACACCAGAGGCATTTTAGCTGAGGAAAAACTCACATTTGAAACATCTGACGACGGTTTAATCTCTAAACAAAACATTGTTTTCGGATGCGGACAAGATAACTCCGGTTTCACCAAATATAGCGGCGTGCTCGGTTTAGGTCCAGGTACATTCTCCATAGTAACCCGCAATTTCGGCTCTAAGTTCTCGTACTGCTTCGGCAGCCTAACCAATCCGACTTACCCTCACAACATTCTAATCCTCGGAAACGGCGCCAAAATCGAAGGCGATCCAACACCTTTACAAATATTCCAAGACCGTTACTACCTTGACCTCCAAGCAATCTCCTTTGGAGAGAAACTTCTCGATATCGAACCCGGAACTTTCCAGAGATATCGGTCCCAAGGAGGCACGGTCATCGACACAGGCTGTTCTCCAACGATTCTAGCCAGAGAAGCTTACGAAACTCTCTCTGAAGAAATTGATTTTCTTCTAGGAGAAGTTCTCAGACGCGTTAAAGATTGGGATCAATACACGACTCCTTGCTACGAAGGGAATCTTAAACTTGATCTCTACGGCTTTCCCGTCGTCACGTTCCATTTCGCCGGCGGCGCTGAGCTGGCGTTAGACGTTGAGAGTTTGTTCGTTAGTAGTGAAAGTGGAGATAGCTTTTGCTTGGCTATGACTATGAACACGTTTGATGATATGAGCGTTATTGGTGCCATGGCTCAGCAGAATTATAATGTTGGCTATAATCTTCGAACCATGAAGGTTTATTTCCAGAGAACTGATTGTGAGATTATTGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

424

Amino Acids

47.23

Weight (kDa)

5.87

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 78 - 244 1.3e-42 Xylanase inhibitor N-terminal
Asp PF00026 78 - 130 5.7e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 261 - 415 6.5e-24 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23945 AT4G30030 AT4G30040
fragaria_vesca FvH4_4g07480
malus_domestica MD16G1251100.v1.1 MD16G1251300.v1.1
prunus_persica Prupe.1G077600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399921
rosa_rugosa Rorug04G0020200
rosa_samantha Rh4BG094700 Rh4CG106400 Rh4DG091200
rosa_wichuraiana Rw4G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1203
AccB1I GGYRCC 2 cut(s) 734, 1181
AccII CGCG 3 cut(s) 432, 474, 975
AciI CCGC 8 cut(s) 168, 269, 402, 472, 573, 606, 649, 1071
AclWI GGATC 4 cut(s) 304, 317, 746, 996
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 957
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 2 cut(s) 631, 674
AflIII ACRYGT 3 cut(s) 202, 973, 1155
AgsI TTSAA 3 cut(s) 72, 366, 524
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 4 cut(s) 503, 920, 1081, 1131
AluI AGCT 4 cut(s) 503, 920, 1081, 1131
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 4 cut(s) 304, 317, 746, 996
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 227, 684
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 2 cut(s) 737, 1076
AspS9I GGNCC 3 cut(s) 192, 623, 861
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 2 cut(s) 452, 586
AsuII TTCGAA 1 cut(s) 1222
AvaII GGWCC 3 cut(s) 192, 623, 861
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 309
BanI GGYRCC 2 cut(s) 734, 1181
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 615
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 2 cut(s) 239, 696
BccI CCATC 1 cut(s) 14
BceAI ACGGC 2 cut(s) 748, 1054
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BciT130I CCWGG 1 cut(s) 627
BciVI GTATCC 1 cut(s) 409
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 3 cut(s) 179, 908, 956
BfoI RGCGCY 2 cut(s) 738, 1077
BfuI GTATCC 1 cut(s) 409
BglII AGATCT 1 cut(s) 368
BisI GCNGC 4 cut(s) 228, 607, 685, 1072
BlpI GCTNAGC 2 cut(s) 1077, 1191
BlsI GCNGC 4 cut(s) 229, 608, 686, 1073
Bme1390I CCNGG 2 cut(s) 627, 840
Bme18I GGWCC 3 cut(s) 192, 623, 861
BmgT120I GGNCC 3 cut(s) 192, 623, 861
BmiI GGNNCC 4 cut(s) 311, 736, 863, 1183
BmrFI CCNGG 2 cut(s) 627, 840
BmsI GCATC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 311
BplI GAGNNNNNCTC 2 cut(s) 951, 983
Bpu10I CCTNAGC 1 cut(s) 504
Bpu1102I GCTNAGC 2 cut(s) 1077, 1191
Bpu14I TTCGAA 1 cut(s) 1222
BpuMI CCSGG 1 cut(s) 840
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 724, 865, 1185
BsaWI WCCGGW 1 cut(s) 587
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 1 cut(s) 448
Bse8I GATNNNNATC 1 cut(s) 987
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 3 cut(s) 724, 865, 1185
BseGI GGATG 1 cut(s) 575
BseJI GATNNNNATC 1 cut(s) 987
BseMII CTCAG 4 cut(s) 495, 982, 1068, 1205
BseNI ACTGG 1 cut(s) 448
BseXI GCAGC 2 cut(s) 239, 696
Bsh1236I CGCG 3 cut(s) 432, 474, 975
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 734, 1181
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 3 cut(s) 588, 840, 1068
BslFI GGGAC 2 cut(s) 251, 847
BsmAI GTCTC 1 cut(s) 202
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 251, 847
BsmI GAATGC 4 cut(s) 83, 88, 151, 230
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 1222
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 5 cut(s) 309, 368, 751, 988, 1030
Bsp1720I GCTNAGC 2 cut(s) 1077, 1191
Bsp19I CCATGG 1 cut(s) 1185
Bsp68I TCGCGA 1 cut(s) 432
BspACI CCGC 8 cut(s) 168, 269, 402, 472, 573, 606, 649, 1071
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 496, 981, 1069, 1204
BspFNI CGCG 3 cut(s) 432, 474, 975
BspLI GGNNCC 4 cut(s) 311, 736, 863, 1183
BspPI GGATC 4 cut(s) 304, 317, 746, 996
BspT104I TTCGAA 1 cut(s) 1222
BspT107I GGYRCC 2 cut(s) 734, 1181
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 3 cut(s) 724, 865, 1185
BssMI GATC 5 cut(s) 309, 368, 751, 988, 1030
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 865, 1185
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 196, 539, 782, 877
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 1222
BstC8I GCNNGC 3 cut(s) 611, 1069, 1083
BstDEI CTNAG 6 cut(s) 104, 504, 663, 968, 1077, 1191
BstDSI CCRYGG 1 cut(s) 1185
BstF5I GGATG 1 cut(s) 575
BstFNI CGCG 3 cut(s) 432, 474, 975
BstH2I RGCGCY 2 cut(s) 738, 1077
BstHHI GCGC 2 cut(s) 737, 1076
BstKTI GATC 5 cut(s) 312, 371, 754, 991, 1033
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 5 cut(s) 309, 368, 751, 988, 1030
BstMWI GCNNNNNNNGC 7 cut(s) 236, 410, 657, 684, 917, 1073, 1082
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 625, 838
BstSNI TACGTA 1 cut(s) 26
BstUI CGCG 3 cut(s) 432, 474, 975
BstV1I GCAGC 2 cut(s) 239, 696
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 2 cut(s) 309, 368
BstYI RGATCY 2 cut(s) 309, 368
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 1185
BtsCI GGATG 1 cut(s) 575
BtuMI TCGCGA 1 cut(s) 432
Cac8I GCNNGC 3 cut(s) 611, 1069, 1083
CfoI GCGC 2 cut(s) 737, 1076
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 3 cut(s) 192, 623, 861
CseI GACGC 1 cut(s) 981
Csp6I GTAC 2 cut(s) 630, 673
CviAII CATG 4 cut(s) 203, 415, 1186, 1228
CviQI GTAC 2 cut(s) 630, 673
DdeI CTNAG 6 cut(s) 104, 504, 663, 968, 1077, 1191
DinI GGCGCC 1 cut(s) 736
DpnI GATC 5 cut(s) 311, 370, 753, 990, 1032
DpnII GATC 5 cut(s) 309, 368, 751, 988, 1030
EaeI YGGCCR 1 cut(s) 3
Eco105I TACGTA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 865, 1185
Eco32I GATATC 2 cut(s) 832, 857
Eco47I GGWCC 3 cut(s) 192, 623, 861
Eco57I CTGAAG 1 cut(s) 957
EcoRII CCWGG 1 cut(s) 625
EcoRV GATATC 2 cut(s) 832, 857
EcoT14I CCWWGG 2 cut(s) 865, 1185
EgeI GGCGCC 1 cut(s) 736
EheI GGCGCC 1 cut(s) 736
ErhI CCWWGG 2 cut(s) 865, 1185
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 4 cut(s) 206, 418, 1189, 1231
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 2 cut(s) 251, 847
FatI CATG 4 cut(s) 202, 414, 1185, 1227
FauI CCCGC 3 cut(s) 175, 276, 656
Fnu4HI GCNGC 4 cut(s) 228, 607, 685, 1072
FokI GGATG 1 cut(s) 582
Fsp4HI GCNGC 4 cut(s) 228, 607, 685, 1072
FspBI CTAG 3 cut(s) 179, 908, 956
GlaI GCGC 2 cut(s) 736, 1075
GluI GCNGC 4 cut(s) 228, 607, 685, 1072
HaeII RGCGCY 2 cut(s) 738, 1077
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 588, 840, 1068
HgaI GACGC 1 cut(s) 981
HhaI GCGC 2 cut(s) 737, 1076
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 4 cut(s) 206, 418, 1189, 1231
Hin6I GCGC 2 cut(s) 735, 1074
HinP1I GCGC 2 cut(s) 735, 1074
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 918
HinfI GANTC 6 cut(s) 122, 220, 904, 1000, 1018, 1268
HpaII CCGG 3 cut(s) 588, 840, 1068
HphI GGTGA 2 cut(s) 452, 586
Hpy166II GTNNAC 2 cut(s) 162, 192
Hpy188I TCNGA 7 cut(s) 219, 532, 569, 699, 728, 937, 971
Hpy188III TCNNGA 6 cut(s) 93, 366, 431, 827, 850, 1243
Hpy8I GTNNAC 2 cut(s) 162, 192
Hpy99I CGWCG 3 cut(s) 437, 539, 1052
HpyAV CCTTC 5 cut(s) 50, 372, 740, 1007, 1225
HpyCH4III ACNGT 4 cut(s) 196, 539, 782, 877
HpyCH4IV ACGT 4 cut(s) 25, 1055, 1092, 1157
HpyCH4V TGCA 4 cut(s) 81, 151, 239, 327
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 410, 657, 684, 917, 1073, 1082
HpyF3I CTNAG 6 cut(s) 104, 504, 663, 968, 1077, 1191
HpySE526I ACGT 4 cut(s) 25, 1055, 1092, 1157
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 4 cut(s) 206, 418, 1189, 1231
HspAI GCGC 2 cut(s) 735, 1074
KasI GGCGCC 1 cut(s) 734
KroI GCCGGC 1 cut(s) 1067
KroNI GCCGGC 1 cut(s) 1069
Kzo9I GATC 5 cut(s) 309, 368, 751, 988, 1030
Lsp1109I GCAGC 2 cut(s) 239, 696
LweI GCATC 1 cut(s) 560
MaeI CTAG 3 cut(s) 179, 908, 956
MaeII ACGT 4 cut(s) 25, 1055, 1092, 1157
MaeIII GTNAC 7 cut(s) 143, 205, 296, 332, 643, 782, 1051
MalI GATC 5 cut(s) 311, 370, 753, 990, 1032
MboI GATC 5 cut(s) 309, 368, 751, 988, 1030
MboII GAAGA 5 cut(s) 311, 363, 944, 950, 1211
MflI RGATCY 2 cut(s) 309, 368
MhlI GDGCHC 1 cut(s) 615
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 652, 942, 1198
MluI ACGCGT 1 cut(s) 973
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 735
MlyI GAGTC 1 cut(s) 994
MmeI TCCRAC 3 cut(s) 722, 779, 923
MnlI CCTC 9 cut(s) 46, 94, 348, 485, 499, 717, 734, 806, 863
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 1067
MroNI GCCGGC 1 cut(s) 1067
MroXI GAANNNNTTC 1 cut(s) 388
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 542, 978, 1023, 1273
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 588, 840, 1068
MspR9I CCNGG 2 cut(s) 627, 840
Mva1269I GAATGC 4 cut(s) 83, 88, 151, 230
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 3 cut(s) 432, 474, 975
MwoI GCNNNNNNNGC 7 cut(s) 236, 410, 657, 684, 917, 1073, 1082
NaeI GCCGGC 1 cut(s) 1069
NarI GGCGCC 1 cut(s) 735
NciI CCSGG 1 cut(s) 840
NcoI CCATGG 1 cut(s) 1185
NdeII GATC 5 cut(s) 309, 368, 751, 988, 1030
NgoMIV GCCGGC 1 cut(s) 1067
NlaIII CATG 4 cut(s) 206, 418, 1189, 1231
NlaIV GGNNCC 4 cut(s) 311, 736, 863, 1183
NmuCI GTSAC 3 cut(s) 205, 296, 1051
NruI TCGCGA 1 cut(s) 432
NspI RCATGY 1 cut(s) 206
NspV TTCGAA 1 cut(s) 1222
PciI ACATGT 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 1044
PctI GAATGC 4 cut(s) 83, 88, 151, 230
PdiI GCCGGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 5 cut(s) 122, 220, 904, 1018, 1268
PkrI GCNGC 4 cut(s) 229, 608, 686, 1073
PleI GAGTC 1 cut(s) 994
PluTI GGCGCC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 994
Ppu21I YACGTR 1 cut(s) 26
PscI ACATGT 1 cut(s) 202
PsiI TTATAA 1 cut(s) 1203
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 4 cut(s) 311, 736, 863, 1183
PspPI GGNCC 3 cut(s) 192, 623, 861
PsuI RGATCY 2 cut(s) 309, 368
RruI TCGCGA 1 cut(s) 432
RsaI GTAC 2 cut(s) 631, 674
RsaNI GTAC 2 cut(s) 630, 673
SaqAI TTAA 4 cut(s) 542, 978, 1023, 1273
SatI GCNGC 4 cut(s) 228, 607, 685, 1072
Sau3AI GATC 5 cut(s) 309, 368, 751, 988, 1030
Sau96I GGNCC 3 cut(s) 192, 623, 861
SchI GAGTC 1 cut(s) 994
ScrFI CCNGG 2 cut(s) 627, 840
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 1 cut(s) 560
SfoI GGCGCC 1 cut(s) 736
SfuI TTCGAA 1 cut(s) 1222
SgrAI CRCCGGYG 1 cut(s) 1067
SinI GGWCC 3 cut(s) 192, 623, 861
SnaBI TACGTA 1 cut(s) 26
Sse9I AATT 3 cut(s) 652, 942, 1198
SsiI CCGC 8 cut(s) 168, 269, 402, 472, 573, 606, 649, 1071
SspDI GGCGCC 1 cut(s) 734
SspI AATATT 2 cut(s) 244, 771
SspMI CTAG 3 cut(s) 179, 908, 956
StyD4I CCNGG 2 cut(s) 625, 838
StyI CCWWGG 2 cut(s) 865, 1185
TaaI ACNGT 4 cut(s) 196, 539, 782, 877
TaiI ACGT 4 cut(s) 28, 1058, 1095, 1160
TaqI TCGA 6 cut(s) 128, 744, 828, 834, 882, 1222
TaqII GACCGA 1 cut(s) 849
TasI AATT 3 cut(s) 652, 942, 1198
TauI GCSGC 2 cut(s) 609, 1074
TfiI GAWTC 5 cut(s) 122, 220, 904, 1018, 1268
Tru1I TTAA 4 cut(s) 542, 978, 1023, 1273
Tru9I TTAA 4 cut(s) 542, 978, 1023, 1273
TseFI GTSAC 3 cut(s) 205, 296, 1051
TseI GCWGC 2 cut(s) 227, 684
Tsp45I GTSAC 3 cut(s) 205, 296, 1051
TspDTI ATGAA 2 cut(s) 1166, 1244
VpaK11BI GGWCC 3 cut(s) 192, 623, 861
XceI RCATGY 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 3 cut(s) 179, 908, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.