Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 239 |
| AciI | CCGC | 5 cut(s) | 21, 74, 111, 143, 208 |
| AclWI | GGATC | 3 cut(s) | 103, 134, 160 |
| AcuI | CTGAAG | 1 cut(s) | 290 |
| AfaI | GTAC | 2 cut(s) | 19, 31 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 45, 73, 110, 149, 425 |
| AgsI | TTSAA | 1 cut(s) | 355 |
| AjnI | CCWGG | 3 cut(s) | 69, 103, 181 |
| AluBI | AGCT | 2 cut(s) | 117, 416 |
| AluI | AGCT | 2 cut(s) | 117, 416 |
| Alw21I | GWGCWC | 1 cut(s) | 418 |
| AlwI | GGATC | 3 cut(s) | 103, 134, 160 |
| AoxI | GGCC | 2 cut(s) | 4, 330 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 392, 424 |
| AspS9I | GGNCC | 1 cut(s) | 4 |
| AsuHPI | GGTGA | 1 cut(s) | 90 |
| BanII | GRGCYC | 1 cut(s) | 418 |
| Bbv12I | GWGCWC | 1 cut(s) | 418 |
| BbvCI | CCTCAGC | 1 cut(s) | 86 |
| BciT130I | CCWGG | 3 cut(s) | 71, 105, 183 |
| Bme1390I | CCNGG | 3 cut(s) | 71, 105, 183 |
| BmgT120I | GGNCC | 1 cut(s) | 4 |
| BmrFI | CCNGG | 3 cut(s) | 71, 105, 183 |
| Bpu10I | CCTNAGC | 2 cut(s) | 86, 430 |
| Bsa29I | ATCGAT | 1 cut(s) | 138 |
| BsaJI | CCNNGG | 2 cut(s) | 103, 253 |
| BsaWI | WCCGGW | 1 cut(s) | 149 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 203, 233 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 45, 73, 110, 149, 425 |
| Bse3DI | GCAATG | 1 cut(s) | 247 |
| BseBI | CCWGG | 3 cut(s) | 71, 105, 183 |
| BseCI | ATCGAT | 1 cut(s) | 138 |
| BseDI | CCNNGG | 2 cut(s) | 103, 253 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 45, 73, 110, 149, 425 |
| BseMI | GCAATG | 1 cut(s) | 247 |
| BseMII | CTCAG | 1 cut(s) | 100 |
| BsgI | GTGCAG | 1 cut(s) | 209 |
| BshFI | GGCC | 2 cut(s) | 6, 332 |
| BshVI | ATCGAT | 1 cut(s) | 138 |
| BsiHKAI | GWGCWC | 1 cut(s) | 418 |
| BsiSI | CCGG | 1 cut(s) | 150 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 45, 73, 110, 149, 425 |
| BsnI | GGCC | 2 cut(s) | 6, 332 |
| Bsp1286I | GDGCHC | 1 cut(s) | 418 |
| Bsp143I | GATC | 4 cut(s) | 95, 139, 152, 310 |
| BspACI | CCGC | 5 cut(s) | 21, 74, 111, 143, 208 |
| BspANI | GGCC | 2 cut(s) | 6, 332 |
| BspCNI | CTCAG | 1 cut(s) | 99 |
| BspDI | ATCGAT | 1 cut(s) | 138 |
| BspPI | GGATC | 3 cut(s) | 103, 134, 160 |
| BspQI | GCTCTTC | 1 cut(s) | 353 |
| BsrDI | GCAATG | 1 cut(s) | 247 |
| BssECI | CCNNGG | 2 cut(s) | 103, 253 |
| BssMI | GATC | 4 cut(s) | 95, 139, 152, 310 |
| Bst2UI | CCWGG | 3 cut(s) | 71, 105, 183 |
| Bst4CI | ACNGT | 2 cut(s) | 342, 424 |
| Bst6I | CTCTTC | 1 cut(s) | 353 |
| BstC8I | GCNNGC | 2 cut(s) | 76, 387 |
| BstDEI | CTNAG | 2 cut(s) | 86, 430 |
| BstKTI | GATC | 4 cut(s) | 98, 142, 155, 313 |
| BstMBI | GATC | 4 cut(s) | 95, 139, 152, 310 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 435 |
| BstNI | CCWGG | 3 cut(s) | 71, 105, 183 |
| BstNSI | RCATGY | 2 cut(s) | 238, 389 |
| BstSCI | CCNGG | 3 cut(s) | 69, 103, 181 |
| Bsu15I | ATCGAT | 1 cut(s) | 138 |
| BsuRI | GGCC | 2 cut(s) | 6, 332 |
| BsuTUI | ATCGAT | 1 cut(s) | 138 |
| Cac8I | GCNNGC | 2 cut(s) | 76, 387 |
| Cfr13I | GGNCC | 1 cut(s) | 4 |
| ClaI | ATCGAT | 1 cut(s) | 138 |
| CseI | GACGC | 1 cut(s) | 79 |
| Csp6I | GTAC | 2 cut(s) | 18, 30 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 31 |
| CviAII | CATG | 3 cut(s) | 200, 235, 386 |
| CviJI | RGCY | 7 cut(s) | 6, 78, 117, 332, 416, 429, 438 |
| CviKI_1 | RGCY | 7 cut(s) | 6, 78, 117, 332, 416, 429, 438 |
| CviQI | GTAC | 2 cut(s) | 18, 30 |
| DdeI | CTNAG | 2 cut(s) | 86, 430 |
| DpnI | GATC | 4 cut(s) | 97, 141, 154, 312 |
| DpnII | GATC | 4 cut(s) | 95, 139, 152, 310 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 239 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 239 |
| Eam1104I | CTCTTC | 1 cut(s) | 353 |
| EarI | CTCTTC | 1 cut(s) | 353 |
| EciI | GGCGGA | 1 cut(s) | 132 |
| Ecl136II | GAGCTC | 1 cut(s) | 416 |
| Eco24I | GRGCYC | 1 cut(s) | 418 |
| Eco53kI | GAGCTC | 1 cut(s) | 416 |
| Eco57I | CTGAAG | 1 cut(s) | 290 |
| EcoICRI | GAGCTC | 1 cut(s) | 416 |
| EcoRII | CCWGG | 3 cut(s) | 69, 103, 181 |
| EcoT38I | GRGCYC | 1 cut(s) | 418 |
| FaeI | CATG | 3 cut(s) | 203, 238, 389 |
| FaiI | YATR | 5 cut(s) | 56, 201, 236, 289, 387 |
| FatI | CATG | 3 cut(s) | 199, 234, 385 |
| FauI | CCCGC | 2 cut(s) | 67, 201 |
| FriOI | GRGCYC | 1 cut(s) | 418 |
| HaeIII | GGCC | 2 cut(s) | 6, 332 |
| HapII | CCGG | 1 cut(s) | 150 |
| HgaI | GACGC | 1 cut(s) | 79 |
| Hin1II | CATG | 3 cut(s) | 203, 238, 389 |
| HincII | GTYRAC | 1 cut(s) | 162 |
| HindII | GTYRAC | 1 cut(s) | 162 |
| HindIII | AAGCTT | 1 cut(s) | 115 |
| HinfI | GANTC | 2 cut(s) | 178, 196 |
| HpaI | GTTAAC | 1 cut(s) | 162 |
| HpaII | CCGG | 1 cut(s) | 150 |
| HphI | GGTGA | 1 cut(s) | 90 |
| Hpy166II | GTNNAC | 1 cut(s) | 162 |
| Hpy188I | TCNGA | 1 cut(s) | 95 |
| Hpy188III | TCNNGA | 2 cut(s) | 398, 432 |
| Hpy8I | GTNNAC | 1 cut(s) | 162 |
| Hpy99I | CGWCG | 1 cut(s) | 95 |
| HpyAV | CCTTC | 1 cut(s) | 292 |
| HpyCH4III | ACNGT | 2 cut(s) | 342, 424 |
| HpyCH4V | TGCA | 2 cut(s) | 190, 389 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 435 |
| HpyF3I | CTNAG | 2 cut(s) | 86, 430 |
| Hsp92II | CATG | 3 cut(s) | 203, 238, 389 |
| KspAI | GTTAAC | 1 cut(s) | 162 |
| Kzo9I | GATC | 4 cut(s) | 95, 139, 152, 310 |
| LguI | GCTCTTC | 1 cut(s) | 353 |
| LmnI | GCTCC | 2 cut(s) | 413, 421 |
| MaeIII | GTNAC | 2 cut(s) | 50, 219 |
| MalI | GATC | 4 cut(s) | 97, 141, 154, 312 |
| MboI | GATC | 4 cut(s) | 95, 139, 152, 310 |
| MboII | GAAGA | 2 cut(s) | 367, 370 |
| MhlI | GDGCHC | 1 cut(s) | 418 |
| MluCI | AATT | 1 cut(s) | 34 |
| MlyI | GAGTC | 2 cut(s) | 187, 205 |
| MmeI | TCCRAC | 3 cut(s) | 63, 73, 268 |
| MnlI | CCTC | 7 cut(s) | 95, 205, 248, 276, 322, 375, 428 |
| MseI | TTAA | 1 cut(s) | 161 |
| MspI | CCGG | 1 cut(s) | 150 |
| MspR9I | CCNGG | 3 cut(s) | 71, 105, 183 |
| MvaI | CCWGG | 3 cut(s) | 71, 105, 183 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 435 |
| NdeII | GATC | 4 cut(s) | 95, 139, 152, 310 |
| NlaIII | CATG | 3 cut(s) | 203, 238, 389 |
| NmeAIII | GCCGAG | 1 cut(s) | 278 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| NspI | RCATGY | 2 cut(s) | 238, 389 |
| PaeI | GCATGC | 1 cut(s) | 389 |
| PciSI | GCTCTTC | 1 cut(s) | 353 |
| PleI | GAGTC | 2 cut(s) | 186, 204 |
| PpsI | GAGTC | 2 cut(s) | 186, 204 |
| Psp124BI | GAGCTC | 1 cut(s) | 418 |
| Psp6I | CCWGG | 3 cut(s) | 69, 103, 181 |
| PspGI | CCWGG | 3 cut(s) | 69, 103, 181 |
| PspPI | GGNCC | 1 cut(s) | 4 |
| RsaI | GTAC | 2 cut(s) | 19, 31 |
| RsaNI | GTAC | 2 cut(s) | 18, 30 |
| SacI | GAGCTC | 1 cut(s) | 418 |
| SapI | GCTCTTC | 1 cut(s) | 353 |
| SaqAI | TTAA | 1 cut(s) | 161 |
| Sau3AI | GATC | 4 cut(s) | 95, 139, 152, 310 |
| Sau96I | GGNCC | 1 cut(s) | 4 |
| SchI | GAGTC | 2 cut(s) | 187, 205 |
| ScrFI | CCNGG | 3 cut(s) | 71, 105, 183 |
| SduI | GDGCHC | 1 cut(s) | 418 |
| SphI | GCATGC | 1 cut(s) | 389 |
| Sse9I | AATT | 1 cut(s) | 34 |
| SsiI | CCGC | 5 cut(s) | 21, 74, 111, 143, 208 |
| SstI | GAGCTC | 1 cut(s) | 418 |
| StyD4I | CCNGG | 3 cut(s) | 69, 103, 181 |
| TaaI | ACNGT | 2 cut(s) | 342, 424 |
| TaqI | TCGA | 2 cut(s) | 138, 176 |
| TasI | AATT | 1 cut(s) | 34 |
| Tru1I | TTAA | 1 cut(s) | 161 |
| Tru9I | TTAA | 1 cut(s) | 161 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspGWI | ACGGA | 2 cut(s) | 245, 394 |
| XceI | RCATGY | 2 cut(s) | 238, 389 |