acetate--CoA ligase ACS, chloroplastic
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AciI | CCGC | 2 cut(s) | 251, 798 |
| AclWI | GGATC | 1 cut(s) | 214 |
| AfaI | GTAC | 3 cut(s) | 522, 678, 1038 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 937, 978 |
| AflIII | ACRYGT | 1 cut(s) | 40 |
| AgsI | TTSAA | 7 cut(s) | 12, 66, 238, 350, 763, 965, 1027 |
| AjnI | CCWGG | 1 cut(s) | 152 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1026, 1058 |
| AleI | CACNNNNGTG | 1 cut(s) | 326 |
| AluBI | AGCT | 3 cut(s) | 72, 189, 643 |
| AluI | AGCT | 3 cut(s) | 72, 189, 643 |
| Alw21I | GWGCWC | 1 cut(s) | 191 |
| Alw26I | GTCTC | 3 cut(s) | 86, 444, 541 |
| AlwI | GGATC | 1 cut(s) | 214 |
| AoxI | GGCC | 1 cut(s) | 148 |
| ApaI | GGGCCC | 1 cut(s) | 152 |
| ApeKI | GCWGC | 2 cut(s) | 125, 953 |
| AseI | ATTAAT | 1 cut(s) | 630 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 42 |
| AspS9I | GGNCC | 2 cut(s) | 148, 149 |
| AsuHPI | GGTGA | 1 cut(s) | 797 |
| BaeGI | GKGCMC | 1 cut(s) | 152 |
| BanII | GRGCYC | 2 cut(s) | 152, 191 |
| Bbv12I | GWGCWC | 1 cut(s) | 191 |
| BbvCI | CCTCAGC | 1 cut(s) | 639 |
| BbvI | GCAGC | 2 cut(s) | 112, 940 |
| BccI | CCATC | 1 cut(s) | 1069 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 284, 318 |
| BciT130I | CCWGG | 1 cut(s) | 154 |
| BclI | TGATCA | 1 cut(s) | 891 |
| BcoDI | GTCTC | 3 cut(s) | 86, 444, 541 |
| BfaI | CTAG | 3 cut(s) | 192, 231, 573 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BisI | GCNGC | 2 cut(s) | 126, 954 |
| BlsI | GCNGC | 2 cut(s) | 127, 955 |
| BmcAI | AGTACT | 1 cut(s) | 1038 |
| Bme1390I | CCNGG | 1 cut(s) | 154 |
| BmgT120I | GGNCC | 2 cut(s) | 148, 149 |
| BmiI | GGNNCC | 2 cut(s) | 150, 151 |
| BmrFI | CCNGG | 1 cut(s) | 154 |
| BmsI | GCATC | 3 cut(s) | 147, 286, 778 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 604, 636 |
| BpmI | CTGGAG | 1 cut(s) | 720 |
| Bpu10I | CCTNAGC | 1 cut(s) | 639 |
| BpuEI | CTTGAG | 1 cut(s) | 204 |
| BsaBI | GATNNNNATC | 1 cut(s) | 876 |
| BsaJI | CCNNGG | 1 cut(s) | 152 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 937, 978 |
| Bse1I | ACTGG | 1 cut(s) | 300 |
| Bse3DI | GCAATG | 2 cut(s) | 600, 907 |
| Bse8I | GATNNNNATC | 1 cut(s) | 876 |
| BseBI | CCWGG | 1 cut(s) | 154 |
| BseDI | CCNNGG | 1 cut(s) | 152 |
| BseGI | GGATG | 5 cut(s) | 162, 538, 927, 1060, 1080 |
| BseJI | GATNNNNATC | 1 cut(s) | 876 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 937, 978 |
| BseMI | GCAATG | 2 cut(s) | 600, 907 |
| BseMII | CTCAG | 2 cut(s) | 21, 653 |
| BseNI | ACTGG | 1 cut(s) | 300 |
| BseSI | GKGCMC | 1 cut(s) | 152 |
| BseXI | GCAGC | 2 cut(s) | 112, 940 |
| BsgI | GTGCAG | 1 cut(s) | 1124 |
| BshFI | GGCC | 1 cut(s) | 150 |
| BsiHKAI | GWGCWC | 1 cut(s) | 191 |
| BslFI | GGGAC | 1 cut(s) | 413 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 937, 978 |
| BsmAI | GTCTC | 3 cut(s) | 86, 444, 541 |
| BsmFI | GGGAC | 1 cut(s) | 413 |
| BsmI | GAATGC | 1 cut(s) | 694 |
| BsnI | GGCC | 1 cut(s) | 150 |
| Bsp120I | GGGCCC | 1 cut(s) | 148 |
| Bsp1286I | GDGCHC | 2 cut(s) | 152, 191 |
| Bsp143I | GATC | 3 cut(s) | 206, 766, 891 |
| BspACI | CCGC | 2 cut(s) | 251, 798 |
| BspANI | GGCC | 1 cut(s) | 150 |
| BspCNI | CTCAG | 2 cut(s) | 22, 652 |
| BspHI | TCATGA | 1 cut(s) | 162 |
| BspLI | GGNNCC | 2 cut(s) | 150, 151 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BspPI | GGATC | 1 cut(s) | 214 |
| BsrDI | GCAATG | 2 cut(s) | 600, 907 |
| BsrI | ACTGG | 1 cut(s) | 300 |
| BssECI | CCNNGG | 1 cut(s) | 152 |
| BssMI | GATC | 3 cut(s) | 206, 766, 891 |
| Bst2UI | CCWGG | 1 cut(s) | 154 |
| Bst4CI | ACNGT | 1 cut(s) | 731 |
| Bst6I | CTCTTC | 2 cut(s) | 605, 1022 |
| BstC8I | GCNNGC | 4 cut(s) | 249, 366, 750, 841 |
| BstDEI | CTNAG | 6 cut(s) | 30, 393, 585, 623, 639, 921 |
| BstF5I | GGATG | 5 cut(s) | 162, 538, 927, 1060, 1080 |
| BstKTI | GATC | 3 cut(s) | 209, 769, 894 |
| BstMAI | GTCTC | 3 cut(s) | 86, 444, 541 |
| BstMBI | GATC | 3 cut(s) | 206, 766, 891 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 374, 698 |
| BstNI | CCWGG | 1 cut(s) | 154 |
| BstNSI | RCATGY | 1 cut(s) | 44 |
| BstSCI | CCNGG | 1 cut(s) | 152 |
| BstSLI | GKGCMC | 1 cut(s) | 152 |
| BstV1I | GCAGC | 2 cut(s) | 112, 940 |
| BstX2I | RGATCY | 1 cut(s) | 206 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 818 |
| BstYI | RGATCY | 1 cut(s) | 206 |
| BsuRI | GGCC | 1 cut(s) | 150 |
| BtsCI | GGATG | 5 cut(s) | 162, 538, 927, 1060, 1080 |
| BtsI | GCAGTG | 1 cut(s) | 1112 |
| BtsIMutI | CAGTG | 3 cut(s) | 307, 727, 1112 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Cac8I | GCNNGC | 4 cut(s) | 249, 366, 750, 841 |
| CciI | TCATGA | 1 cut(s) | 162 |
| Cfr13I | GGNCC | 2 cut(s) | 148, 149 |
| Csp6I | GTAC | 3 cut(s) | 521, 677, 1037 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 99, 134 |
| CviAII | CATG | 7 cut(s) | 5, 41, 88, 163, 371, 812, 1064 |
| CviQI | GTAC | 3 cut(s) | 521, 677, 1037 |
| DdeI | CTNAG | 6 cut(s) | 30, 393, 585, 623, 639, 921 |
| DpnI | GATC | 3 cut(s) | 208, 768, 893 |
| DpnII | GATC | 3 cut(s) | 206, 766, 891 |
| DraI | TTTAAA | 1 cut(s) | 661 |
| Eam1104I | CTCTTC | 2 cut(s) | 605, 1022 |
| EarI | CTCTTC | 2 cut(s) | 605, 1022 |
| Ecl136II | GAGCTC | 1 cut(s) | 189 |
| Eco24I | GRGCYC | 2 cut(s) | 152, 191 |
| Eco53kI | GAGCTC | 1 cut(s) | 189 |
| EcoICRI | GAGCTC | 1 cut(s) | 189 |
| EcoO109I | RGGNCCY | 1 cut(s) | 149 |
| EcoRII | CCWGG | 1 cut(s) | 152 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| EcoT38I | GRGCYC | 2 cut(s) | 152, 191 |
| FaeI | CATG | 7 cut(s) | 8, 44, 91, 166, 374, 815, 1067 |
| FaqI | GGGAC | 1 cut(s) | 413 |
| FatI | CATG | 7 cut(s) | 4, 40, 87, 162, 370, 811, 1063 |
| FbaI | TGATCA | 1 cut(s) | 891 |
| Fnu4HI | GCNGC | 2 cut(s) | 126, 954 |
| FokI | GGATG | 5 cut(s) | 169, 545, 914, 1067, 1087 |
| FriOI | GRGCYC | 2 cut(s) | 152, 191 |
| Fsp4HI | GCNGC | 2 cut(s) | 126, 954 |
| FspBI | CTAG | 3 cut(s) | 192, 231, 573 |
| GluI | GCNGC | 2 cut(s) | 126, 954 |
| GsuI | CTGGAG | 1 cut(s) | 720 |
| HaeIII | GGCC | 1 cut(s) | 150 |
| Hin1II | CATG | 7 cut(s) | 8, 44, 91, 166, 374, 815, 1067 |
| HinfI | GANTC | 6 cut(s) | 8, 234, 284, 548, 965, 1016 |
| HphI | GGTGA | 1 cut(s) | 797 |
| Hpy166II | GTNNAC | 4 cut(s) | 617, 679, 803, 866 |
| Hpy188I | TCNGA | 3 cut(s) | 31, 514, 882 |
| Hpy188III | TCNNGA | 7 cut(s) | 75, 83, 163, 231, 278, 293, 712 |
| Hpy8I | GTNNAC | 4 cut(s) | 617, 679, 803, 866 |
| HpyAV | CCTTC | 4 cut(s) | 133, 208, 688, 1045 |
| HpyCH4III | ACNGT | 1 cut(s) | 731 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 374, 698 |
| HpyF3I | CTNAG | 6 cut(s) | 30, 393, 585, 623, 639, 921 |
| Hsp92II | CATG | 7 cut(s) | 8, 44, 91, 166, 374, 815, 1067 |
| Ksp22I | TGATCA | 1 cut(s) | 891 |
| Kzo9I | GATC | 3 cut(s) | 206, 766, 891 |
| Lsp1109I | GCAGC | 2 cut(s) | 112, 940 |
| LweI | GCATC | 3 cut(s) | 147, 286, 778 |
| MaeI | CTAG | 3 cut(s) | 192, 231, 573 |
| MaeIII | GTNAC | 1 cut(s) | 725 |
| MalI | GATC | 3 cut(s) | 208, 768, 893 |
| MboI | GATC | 3 cut(s) | 206, 766, 891 |
| MboII | GAAGA | 6 cut(s) | 293, 399, 475, 622, 1039, 1060 |
| MfeI | CAATTG | 1 cut(s) | 324 |
| MflI | RGATCY | 1 cut(s) | 206 |
| MhlI | GDGCHC | 2 cut(s) | 152, 191 |
| MluCI | AATT | 5 cut(s) | 324, 332, 580, 670, 1041 |
| MmeI | TCCRAC | 1 cut(s) | 1117 |
| MnlI | CCTC | 6 cut(s) | 115, 256, 630, 648, 744, 1023 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroXI | GAANNNNTTC | 1 cut(s) | 42 |
| MseI | TTAA | 6 cut(s) | 567, 630, 660, 816, 909, 1001 |
| MslI | CAYNNNNRTG | 2 cut(s) | 326, 816 |
| MspR9I | CCNGG | 1 cut(s) | 154 |
| MunI | CAATTG | 1 cut(s) | 324 |
| Mva1269I | GAATGC | 1 cut(s) | 694 |
| MvaI | CCWGG | 1 cut(s) | 154 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 374, 698 |
| NdeII | GATC | 3 cut(s) | 206, 766, 891 |
| NlaIII | CATG | 7 cut(s) | 8, 44, 91, 166, 374, 815, 1067 |
| NlaIV | GGNNCC | 2 cut(s) | 150, 151 |
| NmuCI | GTSAC | 1 cut(s) | 725 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 44 |
| OliI | CACNNNNGTG | 1 cut(s) | 326 |
| PagI | TCATGA | 1 cut(s) | 162 |
| PciI | ACATGT | 1 cut(s) | 40 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 710 |
| PctI | GAATGC | 1 cut(s) | 694 |
| PdmI | GAANNNNTTC | 1 cut(s) | 42 |
| PfeI | GAWTC | 6 cut(s) | 8, 234, 284, 548, 965, 1016 |
| PkrI | GCNGC | 2 cut(s) | 127, 955 |
| PscI | ACATGT | 1 cut(s) | 40 |
| PshBI | ATTAAT | 1 cut(s) | 630 |
| Psp124BI | GAGCTC | 1 cut(s) | 191 |
| Psp6I | CCWGG | 1 cut(s) | 152 |
| PspGI | CCWGG | 1 cut(s) | 152 |
| PspN4I | GGNNCC | 2 cut(s) | 150, 151 |
| PspOMI | GGGCCC | 1 cut(s) | 148 |
| PspPI | GGNCC | 2 cut(s) | 148, 149 |
| PsuI | RGATCY | 1 cut(s) | 206 |
| RsaI | GTAC | 3 cut(s) | 522, 678, 1038 |
| RsaNI | GTAC | 3 cut(s) | 521, 677, 1037 |
| RseI | CAYNNNNRTG | 2 cut(s) | 326, 816 |
| SacI | GAGCTC | 1 cut(s) | 191 |
| SaqAI | TTAA | 6 cut(s) | 567, 630, 660, 816, 909, 1001 |
| SatI | GCNGC | 2 cut(s) | 126, 954 |
| Sau3AI | GATC | 3 cut(s) | 206, 766, 891 |
| Sau96I | GGNCC | 2 cut(s) | 148, 149 |
| ScaI | AGTACT | 1 cut(s) | 1038 |
| ScrFI | CCNGG | 1 cut(s) | 154 |
| SduI | GDGCHC | 2 cut(s) | 152, 191 |
| SfaNI | GCATC | 3 cut(s) | 147, 286, 778 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 326, 816 |
| SmlI | CTYRAG | 1 cut(s) | 219 |
| SmoI | CTYRAG | 1 cut(s) | 219 |
| Sse9I | AATT | 5 cut(s) | 324, 332, 580, 670, 1041 |
| SsiI | CCGC | 2 cut(s) | 251, 798 |
| SspI | AATATT | 1 cut(s) | 436 |
| SspMI | CTAG | 3 cut(s) | 192, 231, 573 |
| SstI | GAGCTC | 1 cut(s) | 191 |
| StyD4I | CCNGG | 1 cut(s) | 152 |
| TaaI | ACNGT | 1 cut(s) | 731 |
| TaqI | TCGA | 2 cut(s) | 22, 870 |
| TasI | AATT | 5 cut(s) | 324, 332, 580, 670, 1041 |
| TatI | WGTACW | 1 cut(s) | 1036 |
| TfiI | GAWTC | 6 cut(s) | 8, 234, 284, 548, 965, 1016 |
| Tru1I | TTAA | 6 cut(s) | 567, 630, 660, 816, 909, 1001 |
| Tru9I | TTAA | 6 cut(s) | 567, 630, 660, 816, 909, 1001 |
| TscAI | CASTG | 3 cut(s) | 307, 734, 1112 |
| TseFI | GTSAC | 1 cut(s) | 725 |
| TseI | GCWGC | 2 cut(s) | 125, 953 |
| Tsp45I | GTSAC | 1 cut(s) | 725 |
| TspDTI | ATGAA | 1 cut(s) | 1029 |
| TspRI | CASTG | 3 cut(s) | 307, 734, 1112 |
| VspI | ATTAAT | 1 cut(s) | 630 |
| XbaI | TCTAGA | 1 cut(s) | 230 |
| XceI | RCATGY | 1 cut(s) | 44 |
| XmnI | GAANNNNTTC | 1 cut(s) | 42 |
| XspI | CTAG | 3 cut(s) | 192, 231, 573 |
| ZrmI | AGTACT | 1 cut(s) | 1038 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |