Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1677 |
| Acc36I | ACCTGC | 1 cut(s) | 1034 |
| Acc65I | GGTACC | 1 cut(s) | 24 |
| AccB1I | GGYRCC | 2 cut(s) | 24, 1396 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1051, 1366 |
| AccI | GTMKAC | 3 cut(s) | 244, 519, 630 |
| AccII | CGCG | 1 cut(s) | 1038 |
| AciI | CCGC | 6 cut(s) | 261, 314, 670, 673, 949, 1038 |
| AclWI | GGATC | 3 cut(s) | 879, 1082, 1472 |
| AcsI | RAATTY | 2 cut(s) | 93, 1583 |
| AfaI | GTAC | 5 cut(s) | 20, 26, 230, 1364, 1601 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 313, 502, 617, 718, 1032, 1051, 1324, 1366, 1702 |
| AgeI | ACCGGT | 1 cut(s) | 53 |
| AgsI | TTSAA | 5 cut(s) | 92, 413, 842, 889, 1562 |
| AjnI | CCWGG | 5 cut(s) | 933, 1180, 1323, 1365, 1712 |
| AleI | CACNNNNGTG | 1 cut(s) | 1349 |
| Alw21I | GWGCWC | 1 cut(s) | 301 |
| Alw26I | GTCTC | 2 cut(s) | 644, 1337 |
| Alw44I | GTGCAC | 1 cut(s) | 297 |
| AlwI | GGATC | 3 cut(s) | 879, 1082, 1472 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 347, 1026, 1366 |
| Ama87I | CYCGRG | 2 cut(s) | 248, 1347 |
| AoxI | GGCC | 4 cut(s) | 451, 504, 1225, 1304 |
| ApaLI | GTGCAC | 1 cut(s) | 297 |
| ApeKI | GCWGC | 6 cut(s) | 662, 1064, 1106, 1172, 1370, 1424 |
| ApoI | RAATTY | 2 cut(s) | 93, 1583 |
| AsiGI | ACCGGT | 1 cut(s) | 53 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 825 |
| Asp718I | GGTACC | 1 cut(s) | 24 |
| AspLEI | GCGC | 1 cut(s) | 1038 |
| AspS9I | GGNCC | 4 cut(s) | 344, 695, 1023, 1225 |
| AsuC2I | CCSGG | 5 cut(s) | 249, 250, 965, 1348, 1349 |
| AsuHPI | GGTGA | 4 cut(s) | 417, 695, 932, 1132 |
| AvaI | CYCGRG | 2 cut(s) | 248, 1347 |
| AvaII | GGWCC | 3 cut(s) | 344, 695, 1023 |
| AxyI | CCTNAGG | 1 cut(s) | 582 |
| BaeGI | GKGCMC | 1 cut(s) | 301 |
| BanI | GGYRCC | 2 cut(s) | 24, 1396 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1583, 1615 |
| BbsI | GAAGAC | 2 cut(s) | 1296, 1548 |
| Bbv12I | GWGCWC | 1 cut(s) | 301 |
| BbvI | GCAGC | 6 cut(s) | 674, 1076, 1118, 1184, 1382, 1411 |
| BccI | CCATC | 9 cut(s) | 77, 355, 473, 596, 769, 894, 1154, 1481, 1487 |
| BciT130I | CCWGG | 5 cut(s) | 935, 1182, 1325, 1367, 1714 |
| BcnI | CCSGG | 5 cut(s) | 249, 250, 965, 1348, 1349 |
| BcoDI | GTCTC | 2 cut(s) | 644, 1337 |
| BfaI | CTAG | 3 cut(s) | 971, 1308, 1649 |
| BfuAI | ACCTGC | 1 cut(s) | 1034 |
| BisI | GCNGC | 7 cut(s) | 663, 673, 1065, 1107, 1173, 1371, 1425 |
| BlsI | GCNGC | 7 cut(s) | 664, 674, 1066, 1108, 1174, 1372, 1426 |
| Bme18I | GGWCC | 3 cut(s) | 344, 695, 1023 |
| BmeT110I | CYCGRG | 2 cut(s) | 248, 1347 |
| BmgT120I | GGNCC | 4 cut(s) | 344, 695, 1023, 1225 |
| BmiI | GGNNCC | 2 cut(s) | 26, 1398 |
| BmsI | GCATC | 9 cut(s) | 64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715 |
| BoxI | GACNNNNGTC | 1 cut(s) | 636 |
| BpiI | GAAGAC | 2 cut(s) | 1296, 1548 |
| BpmI | CTGGAG | 3 cut(s) | 405, 1346, 1404 |
| Bpu10I | CCTNAGC | 1 cut(s) | 866 |
| BpuMI | CCSGG | 5 cut(s) | 249, 250, 965, 1348, 1349 |
| BsaBI | GATNNNNATC | 2 cut(s) | 365, 1076 |
| BsaI | GGTCTC | 2 cut(s) | 644, 1337 |
| BsaJI | CCNNGG | 6 cut(s) | 248, 679, 1050, 1323, 1347, 1701 |
| BsaWI | WCCGGW | 1 cut(s) | 53 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 378, 408, 1494, 1524 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 313, 502, 617, 718, 1032, 1051, 1324, 1366, 1702 |
| Bse118I | RCCGGY | 1 cut(s) | 53 |
| Bse1I | ACTGG | 6 cut(s) | 302, 622, 1091, 1360, 1387, 1460 |
| Bse21I | CCTNAGG | 1 cut(s) | 582 |
| Bse3DI | GCAATG | 1 cut(s) | 1637 |
| Bse8I | GATNNNNATC | 2 cut(s) | 365, 1076 |
| BseBI | CCWGG | 5 cut(s) | 935, 1182, 1325, 1367, 1714 |
| BseDI | CCNNGG | 6 cut(s) | 248, 679, 1050, 1323, 1347, 1701 |
| BseGI | GGATG | 6 cut(s) | 484, 853, 1177, 1271, 1492, 1709 |
| BseJI | GATNNNNATC | 2 cut(s) | 365, 1076 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 313, 502, 617, 718, 1032, 1051, 1324, 1366, 1702 |
| BseMI | GCAATG | 1 cut(s) | 1637 |
| BseMII | CTCAG | 4 cut(s) | 122, 573, 1082, 1425 |
| BseNI | ACTGG | 6 cut(s) | 302, 622, 1091, 1360, 1387, 1460 |
| BseRI | GAGGAG | 4 cut(s) | 238, 1004, 1012, 1326 |
| BseSI | GKGCMC | 1 cut(s) | 301 |
| BseXI | GCAGC | 6 cut(s) | 674, 1076, 1118, 1184, 1382, 1411 |
| BseYI | CCCAGC | 2 cut(s) | 432, 1192 |
| BsgI | GTGCAG | 1 cut(s) | 15 |
| Bsh1236I | CGCG | 1 cut(s) | 1038 |
| BshFI | GGCC | 4 cut(s) | 453, 506, 1227, 1306 |
| BshNI | GGYRCC | 2 cut(s) | 24, 1396 |
| BshTI | ACCGGT | 1 cut(s) | 53 |
| BsiHKAI | GWGCWC | 1 cut(s) | 301 |
| BsiHKCI | CYCGRG | 2 cut(s) | 248, 1347 |
| BsiSI | CCGG | 5 cut(s) | 54, 249, 964, 1336, 1348 |
| BsiWI | CGTACG | 1 cut(s) | 18 |
| BslFI | GGGAC | 2 cut(s) | 114, 290 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 313, 502, 617, 718, 1032, 1051, 1324, 1366, 1702 |
| BsmAI | GTCTC | 2 cut(s) | 644, 1337 |
| BsmFI | GGGAC | 2 cut(s) | 114, 290 |
| BsmI | GAATGC | 2 cut(s) | 665, 724 |
| BsnI | GGCC | 4 cut(s) | 453, 506, 1227, 1306 |
| Bso31I | GGTCTC | 2 cut(s) | 644, 1337 |
| BsoBI | CYCGRG | 2 cut(s) | 248, 1347 |
| Bsp1286I | GDGCHC | 1 cut(s) | 301 |
| Bsp1407I | TGTACA | 1 cut(s) | 1599 |
| Bsp143I | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| Bsp19I | CCATGG | 1 cut(s) | 1050 |
| BspACI | CCGC | 6 cut(s) | 261, 314, 670, 673, 949, 1038 |
| BspANI | GGCC | 4 cut(s) | 453, 506, 1227, 1306 |
| BspCNI | CTCAG | 4 cut(s) | 123, 574, 1081, 1426 |
| BspFNI | CGCG | 1 cut(s) | 1038 |
| BspHI | TCATGA | 1 cut(s) | 993 |
| BspLI | GGNNCC | 2 cut(s) | 26, 1398 |
| BspMI | ACCTGC | 1 cut(s) | 1034 |
| BspPI | GGATC | 3 cut(s) | 879, 1082, 1472 |
| BspT107I | GGYRCC | 2 cut(s) | 24, 1396 |
| BspTNI | GGTCTC | 2 cut(s) | 644, 1337 |
| BsrDI | GCAATG | 1 cut(s) | 1637 |
| BsrFI | RCCGGY | 1 cut(s) | 53 |
| BsrGI | TGTACA | 1 cut(s) | 1599 |
| BsrI | ACTGG | 6 cut(s) | 302, 622, 1091, 1360, 1387, 1460 |
| BssAI | RCCGGY | 1 cut(s) | 53 |
| BssECI | CCNNGG | 6 cut(s) | 248, 679, 1050, 1323, 1347, 1701 |
| BssMI | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| BssT1I | CCWWGG | 3 cut(s) | 679, 1050, 1701 |
| Bst2UI | CCWGG | 5 cut(s) | 935, 1182, 1325, 1367, 1714 |
| Bst4CI | ACNGT | 3 cut(s) | 105, 811, 893 |
| BstAUI | TGTACA | 1 cut(s) | 1599 |
| BstC8I | GCNNGC | 3 cut(s) | 612, 1198, 1612 |
| BstDEI | CTNAG | 7 cut(s) | 131, 219, 582, 866, 1068, 1136, 1434 |
| BstDSI | CCRYGG | 1 cut(s) | 1050 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1030 |
| BstF5I | GGATG | 6 cut(s) | 484, 853, 1177, 1271, 1492, 1709 |
| BstFNI | CGCG | 1 cut(s) | 1038 |
| BstHHI | GCGC | 1 cut(s) | 1038 |
| BstKTI | GATC | 7 cut(s) | 325, 535, 819, 874, 1012, 1090, 1467 |
| BstMAI | GTCTC | 2 cut(s) | 644, 1337 |
| BstMBI | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 556, 1035, 1103, 1430 |
| BstNI | CCWGG | 5 cut(s) | 935, 1182, 1325, 1367, 1714 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 636 |
| BstSLI | GKGCMC | 1 cut(s) | 301 |
| BstUI | CGCG | 1 cut(s) | 1038 |
| BstV1I | GCAGC | 6 cut(s) | 674, 1076, 1118, 1184, 1382, 1411 |
| BstV2I | GAAGAC | 2 cut(s) | 1296, 1548 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 570 |
| Bsu36I | CCTNAGG | 1 cut(s) | 582 |
| BsuRI | GGCC | 4 cut(s) | 453, 506, 1227, 1306 |
| BtgI | CCRYGG | 1 cut(s) | 1050 |
| BtsCI | GGATG | 6 cut(s) | 484, 853, 1177, 1271, 1492, 1709 |
| BtsI | GCAGTG | 1 cut(s) | 1466 |
| BtsIMutI | CAGTG | 2 cut(s) | 807, 1466 |
| BveI | ACCTGC | 1 cut(s) | 1034 |
| Cac8I | GCNNGC | 3 cut(s) | 612, 1198, 1612 |
| CaiI | CAGNNNCTG | 3 cut(s) | 347, 1026, 1366 |
| CciI | TCATGA | 1 cut(s) | 993 |
| CfoI | GCGC | 1 cut(s) | 1038 |
| Cfr10I | RCCGGY | 1 cut(s) | 53 |
| Cfr13I | GGNCC | 4 cut(s) | 344, 695, 1023, 1225 |
| Cfr9I | CCCGGG | 2 cut(s) | 248, 1347 |
| CseI | GACGC | 1 cut(s) | 548 |
| Csp6I | GTAC | 5 cut(s) | 19, 25, 229, 1363, 1600 |
| CspAI | ACCGGT | 1 cut(s) | 53 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 220, 255 |
| CviQI | GTAC | 5 cut(s) | 19, 25, 229, 1363, 1600 |
| DdeI | CTNAG | 7 cut(s) | 131, 219, 582, 866, 1068, 1136, 1434 |
| DpnI | GATC | 7 cut(s) | 324, 534, 818, 873, 1011, 1089, 1466 |
| DpnII | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| EciI | GGCGGA | 1 cut(s) | 659 |
| Eco130I | CCWWGG | 3 cut(s) | 679, 1050, 1701 |
| Eco147I | AGGCCT | 1 cut(s) | 1306 |
| Eco31I | GGTCTC | 2 cut(s) | 644, 1337 |
| Eco47I | GGWCC | 3 cut(s) | 344, 695, 1023 |
| Eco81I | CCTNAGG | 1 cut(s) | 582 |
| Eco88I | CYCGRG | 2 cut(s) | 248, 1347 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1030 |
| EcoO109I | RGGNCCY | 2 cut(s) | 344, 1023 |
| EcoRII | CCWGG | 5 cut(s) | 933, 1180, 1323, 1365, 1712 |
| EcoT14I | CCWWGG | 3 cut(s) | 679, 1050, 1701 |
| EcoT22I | ATGCAT | 2 cut(s) | 199, 1273 |
| ErhI | CCWWGG | 3 cut(s) | 679, 1050, 1701 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 405, 437 |
| FaqI | GGGAC | 2 cut(s) | 114, 290 |
| FauI | CCCGC | 1 cut(s) | 268 |
| FblI | GTMKAC | 3 cut(s) | 244, 519, 630 |
| Fnu4HI | GCNGC | 7 cut(s) | 663, 673, 1065, 1107, 1173, 1371, 1425 |
| FokI | GGATG | 6 cut(s) | 491, 860, 1164, 1258, 1499, 1696 |
| Fsp4HI | GCNGC | 7 cut(s) | 663, 673, 1065, 1107, 1173, 1371, 1425 |
| FspBI | CTAG | 3 cut(s) | 971, 1308, 1649 |
| GlaI | GCGC | 1 cut(s) | 1037 |
| GluI | GCNGC | 7 cut(s) | 663, 673, 1065, 1107, 1173, 1371, 1425 |
| GsaI | CCCAGC | 2 cut(s) | 436, 1196 |
| GsuI | CTGGAG | 3 cut(s) | 405, 1346, 1404 |
| HaeIII | GGCC | 4 cut(s) | 453, 506, 1227, 1306 |
| HapII | CCGG | 5 cut(s) | 54, 249, 964, 1336, 1348 |
| HgaI | GACGC | 1 cut(s) | 548 |
| HhaI | GCGC | 1 cut(s) | 1038 |
| Hin6I | GCGC | 1 cut(s) | 1036 |
| HinP1I | GCGC | 1 cut(s) | 1036 |
| HincII | GTYRAC | 1 cut(s) | 631 |
| HindII | GTYRAC | 1 cut(s) | 631 |
| HindIII | AAGCTT | 1 cut(s) | 548 |
| HinfI | GANTC | 8 cut(s) | 127, 439, 516, 982, 1072, 1314, 1356, 1663 |
| HpaII | CCGG | 5 cut(s) | 54, 249, 964, 1336, 1348 |
| HphI | GGTGA | 4 cut(s) | 417, 695, 932, 1132 |
| Hpy166II | GTNNAC | 7 cut(s) | 245, 299, 460, 520, 631, 1045, 1623 |
| Hpy8I | GTNNAC | 7 cut(s) | 245, 299, 460, 520, 631, 1045, 1623 |
| Hpy99I | CGWCG | 1 cut(s) | 43 |
| HpyAV | CCTTC | 9 cut(s) | 205, 275, 680, 757, 940, 1120, 1510, 1570, 1585 |
| HpyCH4III | ACNGT | 3 cut(s) | 105, 811, 893 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 556, 1035, 1103, 1430 |
| HpyF3I | CTNAG | 7 cut(s) | 131, 219, 582, 866, 1068, 1136, 1434 |
| HspAI | GCGC | 1 cut(s) | 1036 |
| KpnI | GGTACC | 1 cut(s) | 28 |
| Kzo9I | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| LmnI | GCTCC | 5 cut(s) | 386, 483, 721, 1385, 1519 |
| Lsp1109I | GCAGC | 6 cut(s) | 674, 1076, 1118, 1184, 1382, 1411 |
| LweI | GCATC | 9 cut(s) | 64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715 |
| MaeI | CTAG | 3 cut(s) | 971, 1308, 1649 |
| MaeIII | GTNAC | 1 cut(s) | 1001 |
| MalI | GATC | 7 cut(s) | 324, 534, 818, 873, 1011, 1089, 1466 |
| MboI | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| MhlI | GDGCHC | 1 cut(s) | 301 |
| MluCI | AATT | 4 cut(s) | 93, 379, 748, 1583 |
| MlyI | GAGTC | 3 cut(s) | 525, 1323, 1657 |
| MmeI | TCCRAC | 1 cut(s) | 1464 |
| Mph1103I | ATGCAT | 2 cut(s) | 199, 1273 |
| MroXI | GAANNNNTTC | 1 cut(s) | 825 |
| MseI | TTAA | 2 cut(s) | 189, 1587 |
| MslI | CAYNNNNRTG | 3 cut(s) | 202, 657, 1349 |
| MspA1I | CMGCKG | 2 cut(s) | 833, 1433 |
| MspI | CCGG | 5 cut(s) | 54, 249, 964, 1336, 1348 |
| Mva1269I | GAATGC | 2 cut(s) | 665, 724 |
| MvaI | CCWGG | 5 cut(s) | 935, 1182, 1325, 1367, 1714 |
| MvnI | CGCG | 1 cut(s) | 1038 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 556, 1035, 1103, 1430 |
| NciI | CCSGG | 5 cut(s) | 249, 250, 965, 1348, 1349 |
| NcoI | CCATGG | 1 cut(s) | 1050 |
| NdeII | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| NlaIV | GGNNCC | 2 cut(s) | 26, 1398 |
| NsiI | ATGCAT | 2 cut(s) | 199, 1273 |
| OliI | CACNNNNGTG | 1 cut(s) | 1349 |
| PagI | TCATGA | 1 cut(s) | 993 |
| PceI | AGGCCT | 1 cut(s) | 1306 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 78 |
| PctI | GAATGC | 2 cut(s) | 665, 724 |
| PdmI | GAANNNNTTC | 1 cut(s) | 825 |
| PfeI | GAWTC | 5 cut(s) | 127, 439, 982, 1072, 1356 |
| Pfl23II | CGTACG | 1 cut(s) | 18 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1051, 1366 |
| PfoI | TCCNGGA | 1 cut(s) | 1180 |
| PinAI | ACCGGT | 1 cut(s) | 53 |
| PkrI | GCNGC | 7 cut(s) | 664, 674, 1066, 1108, 1174, 1372, 1426 |
| PleI | GAGTC | 3 cut(s) | 524, 1322, 1657 |
| PpsI | GAGTC | 3 cut(s) | 524, 1322, 1657 |
| PpuMI | RGGWCCY | 2 cut(s) | 344, 1023 |
| PshAI | GACNNNNGTC | 1 cut(s) | 636 |
| PsiI | TTATAA | 1 cut(s) | 1677 |
| Psp5II | RGGWCCY | 2 cut(s) | 344, 1023 |
| Psp6I | CCWGG | 5 cut(s) | 933, 1180, 1323, 1365, 1712 |
| PspFI | CCCAGC | 2 cut(s) | 432, 1192 |
| PspGI | CCWGG | 5 cut(s) | 933, 1180, 1323, 1365, 1712 |
| PspLI | CGTACG | 1 cut(s) | 18 |
| PspN4I | GGNNCC | 2 cut(s) | 26, 1398 |
| PspPI | GGNCC | 4 cut(s) | 344, 695, 1023, 1225 |
| PspPPI | RGGWCCY | 2 cut(s) | 344, 1023 |
| PstNI | CAGNNNCTG | 3 cut(s) | 347, 1026, 1366 |
| PvuII | CAGCTG | 2 cut(s) | 833, 1433 |
| RsaI | GTAC | 5 cut(s) | 20, 26, 230, 1364, 1601 |
| RsaNI | GTAC | 5 cut(s) | 19, 25, 229, 1363, 1600 |
| RseI | CAYNNNNRTG | 3 cut(s) | 202, 657, 1349 |
| SalI | GTCGAC | 1 cut(s) | 629 |
| SaqAI | TTAA | 2 cut(s) | 189, 1587 |
| SatI | GCNGC | 7 cut(s) | 663, 673, 1065, 1107, 1173, 1371, 1425 |
| Sau3AI | GATC | 7 cut(s) | 322, 532, 816, 871, 1009, 1087, 1464 |
| Sau96I | GGNCC | 4 cut(s) | 344, 695, 1023, 1225 |
| SchI | GAGTC | 3 cut(s) | 525, 1323, 1657 |
| SduI | GDGCHC | 1 cut(s) | 301 |
| SfaNI | GCATC | 9 cut(s) | 64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715 |
| SgrAI | CRCCGGYG | 1 cut(s) | 53 |
| SinI | GGWCC | 3 cut(s) | 344, 695, 1023 |
| SmaI | CCCGGG | 2 cut(s) | 250, 1349 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 202, 657, 1349 |
| Sse9I | AATT | 4 cut(s) | 93, 379, 748, 1583 |
| SseBI | AGGCCT | 1 cut(s) | 1306 |
| SsiI | CCGC | 6 cut(s) | 261, 314, 670, 673, 949, 1038 |
| SspMI | CTAG | 3 cut(s) | 971, 1308, 1649 |
| StuI | AGGCCT | 1 cut(s) | 1306 |
| StyI | CCWWGG | 3 cut(s) | 679, 1050, 1701 |
| TaaI | ACNGT | 3 cut(s) | 105, 811, 893 |
| TaqI | TCGA | 4 cut(s) | 630, 759, 819, 1668 |
| TasI | AATT | 4 cut(s) | 93, 379, 748, 1583 |
| TatI | WGTACW | 1 cut(s) | 1599 |
| TauI | GCSGC | 1 cut(s) | 675 |
| TfiI | GAWTC | 5 cut(s) | 127, 439, 982, 1072, 1356 |
| Tru1I | TTAA | 2 cut(s) | 189, 1587 |
| Tru9I | TTAA | 2 cut(s) | 189, 1587 |
| TscAI | CASTG | 2 cut(s) | 814, 1466 |
| TseI | GCWGC | 6 cut(s) | 662, 1064, 1106, 1172, 1370, 1424 |
| TspDTI | ATGAA | 7 cut(s) | 62, 262, 388, 864, 982, 995, 1116 |
| TspMI | CCCGGG | 2 cut(s) | 248, 1347 |
| TspRI | CASTG | 2 cut(s) | 814, 1466 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1051, 1366 |
| VneI | GTGCAC | 1 cut(s) | 297 |
| VpaK11BI | GGWCC | 3 cut(s) | 344, 695, 1023 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1030 |
| XapI | RAATTY | 2 cut(s) | 93, 1583 |
| XbaI | TCTAGA | 1 cut(s) | 1648 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1057 |
| XmaI | CCCGGG | 2 cut(s) | 248, 1347 |
| XmiI | GTMKAC | 3 cut(s) | 244, 519, 630 |
| XmnI | GAANNNNTTC | 1 cut(s) | 825 |
| XspI | CTAG | 3 cut(s) | 971, 1308, 1649 |
| Zsp2I | ATGCAT | 2 cut(s) | 199, 1273 |