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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 20 |
| AccB1I | GGYRCC | 1 cut(s) | 20 |
| AccI | GTMKAC | 1 cut(s) | 114 |
| AciI | CCGC | 1 cut(s) | 630 |
| AclWI | GGATC | 2 cut(s) | 169, 407 |
| AcuI | CTGAAG | 1 cut(s) | 159 |
| AfaI | GTAC | 3 cut(s) | 22, 595, 634 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 179, 236, 459 |
| AgsI | TTSAA | 5 cut(s) | 85, 158, 442, 515, 786 |
| AhlI | ACTAGT | 2 cut(s) | 184, 245 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 153, 185 |
| AluBI | AGCT | 5 cut(s) | 60, 261, 344, 362, 657 |
| AluI | AGCT | 5 cut(s) | 60, 261, 344, 362, 657 |
| Alw26I | GTCTC | 1 cut(s) | 132 |
| AlwI | GGATC | 2 cut(s) | 169, 407 |
| AoxI | GGCC | 3 cut(s) | 34, 41, 567 |
| ApeKI | GCWGC | 3 cut(s) | 201, 344, 362 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 620 |
| Asp718I | GGTACC | 1 cut(s) | 20 |
| AspS9I | GGNCC | 3 cut(s) | 41, 568, 728 |
| AsuNHI | GCTAGC | 1 cut(s) | 653 |
| AvaII | GGWCC | 1 cut(s) | 728 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 12, 45 |
| BanI | GGYRCC | 1 cut(s) | 20 |
| BbvI | GCAGC | 3 cut(s) | 213, 331, 349 |
| BccI | CCATC | 1 cut(s) | 11 |
| BceAI | ACGGC | 1 cut(s) | 366 |
| BciVI | GTATCC | 1 cut(s) | 676 |
| BcoDI | GTCTC | 1 cut(s) | 132 |
| BcuI | ACTAGT | 2 cut(s) | 184, 245 |
| BfaI | CTAG | 3 cut(s) | 185, 246, 654 |
| BfuI | GTATCC | 1 cut(s) | 676 |
| BisI | GCNGC | 3 cut(s) | 202, 345, 363 |
| BlsI | GCNGC | 3 cut(s) | 203, 346, 364 |
| BmcAI | AGTACT | 1 cut(s) | 595 |
| Bme18I | GGWCC | 1 cut(s) | 728 |
| BmgT120I | GGNCC | 3 cut(s) | 41, 568, 728 |
| BmiI | GGNNCC | 3 cut(s) | 22, 134, 570 |
| BmrI | ACTGGG | 1 cut(s) | 611 |
| BmsI | GCATC | 2 cut(s) | 200, 397 |
| BmtI | GCTAGC | 1 cut(s) | 657 |
| BmuI | ACTGGG | 1 cut(s) | 611 |
| Bpu10I | CCTNAGC | 1 cut(s) | 366 |
| BsaBI | GATNNNNATC | 1 cut(s) | 411 |
| BsaI | GGTCTC | 1 cut(s) | 132 |
| BsaJI | CCNNGG | 3 cut(s) | 37, 44, 235 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 179, 236, 459 |
| Bse118I | RCCGGY | 1 cut(s) | 712 |
| Bse1I | ACTGG | 2 cut(s) | 230, 606 |
| Bse8I | GATNNNNATC | 1 cut(s) | 411 |
| BseDI | CCNNGG | 3 cut(s) | 37, 44, 235 |
| BseGI | GGATG | 2 cut(s) | 616, 700 |
| BseJI | GATNNNNATC | 1 cut(s) | 411 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 179, 236, 459 |
| BseMII | CTCAG | 3 cut(s) | 276, 380, 381 |
| BseNI | ACTGG | 2 cut(s) | 230, 606 |
| BseXI | GCAGC | 3 cut(s) | 213, 331, 349 |
| BshFI | GGCC | 3 cut(s) | 36, 43, 569 |
| BshNI | GGYRCC | 1 cut(s) | 20 |
| BsiSI | CCGG | 2 cut(s) | 180, 713 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 179, 236, 459 |
| BsmAI | GTCTC | 1 cut(s) | 132 |
| BsnI | GGCC | 3 cut(s) | 36, 43, 569 |
| Bso31I | GGTCTC | 1 cut(s) | 132 |
| Bsp143I | GATC | 2 cut(s) | 161, 412 |
| Bsp19I | CCATGG | 1 cut(s) | 44 |
| BspACI | CCGC | 1 cut(s) | 630 |
| BspANI | GGCC | 3 cut(s) | 36, 43, 569 |
| BspCNI | CTCAG | 3 cut(s) | 275, 379, 382 |
| BspLI | GGNNCC | 3 cut(s) | 22, 134, 570 |
| BspOI | GCTAGC | 1 cut(s) | 657 |
| BspPI | GGATC | 2 cut(s) | 169, 407 |
| BspT107I | GGYRCC | 1 cut(s) | 20 |
| BspTNI | GGTCTC | 1 cut(s) | 132 |
| BsrFI | RCCGGY | 1 cut(s) | 712 |
| BsrI | ACTGG | 2 cut(s) | 230, 606 |
| BssAI | RCCGGY | 1 cut(s) | 712 |
| BssECI | CCNNGG | 3 cut(s) | 37, 44, 235 |
| BssMI | GATC | 2 cut(s) | 161, 412 |
| BssT1I | CCWWGG | 3 cut(s) | 37, 44, 235 |
| BstC8I | GCNNGC | 3 cut(s) | 144, 396, 655 |
| BstDEI | CTNAG | 3 cut(s) | 262, 366, 390 |
| BstDSI | CCRYGG | 1 cut(s) | 44 |
| BstEII | GGTNACC | 1 cut(s) | 370 |
| BstF5I | GGATG | 2 cut(s) | 616, 700 |
| BstKTI | GATC | 2 cut(s) | 164, 415 |
| BstMAI | GTCTC | 1 cut(s) | 132 |
| BstMBI | GATC | 2 cut(s) | 161, 412 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 350, 359 |
| BstPI | GGTNACC | 1 cut(s) | 370 |
| BstV1I | GCAGC | 3 cut(s) | 213, 331, 349 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 222 |
| BsuI | GTATCC | 1 cut(s) | 676 |
| BsuRI | GGCC | 3 cut(s) | 36, 43, 569 |
| BtgI | CCRYGG | 1 cut(s) | 44 |
| BtsCI | GGATG | 2 cut(s) | 616, 700 |
| Cac8I | GCNNGC | 3 cut(s) | 144, 396, 655 |
| Cfr10I | RCCGGY | 1 cut(s) | 712 |
| Cfr13I | GGNCC | 3 cut(s) | 41, 568, 728 |
| Csp6I | GTAC | 3 cut(s) | 21, 594, 633 |
| CviAII | CATG | 5 cut(s) | 45, 222, 625, 679, 754 |
| CviQI | GTAC | 3 cut(s) | 21, 594, 633 |
| DdeI | CTNAG | 3 cut(s) | 262, 366, 390 |
| DpnI | GATC | 2 cut(s) | 163, 414 |
| DpnII | GATC | 2 cut(s) | 161, 412 |
| Eco130I | CCWWGG | 3 cut(s) | 37, 44, 235 |
| Eco147I | AGGCCT | 1 cut(s) | 36 |
| Eco31I | GGTCTC | 1 cut(s) | 132 |
| Eco47I | GGWCC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 1 cut(s) | 159 |
| Eco91I | GGTNACC | 1 cut(s) | 370 |
| EcoO109I | RGGNCCY | 1 cut(s) | 568 |
| EcoO65I | GGTNACC | 1 cut(s) | 370 |
| EcoT14I | CCWWGG | 3 cut(s) | 37, 44, 235 |
| ErhI | CCWWGG | 3 cut(s) | 37, 44, 235 |
| FaeI | CATG | 5 cut(s) | 48, 225, 628, 682, 757 |
| FaiI | YATR | 9 cut(s) | 46, 63, 223, 251, 626, 680, 755, 771, 776 |
| FatI | CATG | 5 cut(s) | 44, 221, 624, 678, 753 |
| FblI | GTMKAC | 1 cut(s) | 114 |
| Fnu4HI | GCNGC | 3 cut(s) | 202, 345, 363 |
| FokI | GGATG | 2 cut(s) | 623, 687 |
| Fsp4HI | GCNGC | 3 cut(s) | 202, 345, 363 |
| FspBI | CTAG | 3 cut(s) | 185, 246, 654 |
| GluI | GCNGC | 3 cut(s) | 202, 345, 363 |
| HaeIII | GGCC | 3 cut(s) | 36, 43, 569 |
| HapII | CCGG | 2 cut(s) | 180, 713 |
| Hin1II | CATG | 5 cut(s) | 48, 225, 628, 682, 757 |
| HinfI | GANTC | 3 cut(s) | 92, 747, 782 |
| HpaII | CCGG | 2 cut(s) | 180, 713 |
| Hpy166II | GTNNAC | 1 cut(s) | 115 |
| Hpy188III | TCNNGA | 1 cut(s) | 321 |
| Hpy8I | GTNNAC | 1 cut(s) | 115 |
| HpyAV | CCTTC | 5 cut(s) | 103, 152, 183, 564, 601 |
| HpyCH4V | TGCA | 1 cut(s) | 146 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 350, 359 |
| HpyF3I | CTNAG | 3 cut(s) | 262, 366, 390 |
| Hsp92II | CATG | 5 cut(s) | 48, 225, 628, 682, 757 |
| KpnI | GGTACC | 1 cut(s) | 24 |
| Kzo9I | GATC | 2 cut(s) | 161, 412 |
| LmnI | GCTCC | 1 cut(s) | 556 |
| Lsp1109I | GCAGC | 3 cut(s) | 213, 331, 349 |
| LweI | GCATC | 2 cut(s) | 200, 397 |
| MaeI | CTAG | 3 cut(s) | 185, 246, 654 |
| MaeIII | GTNAC | 2 cut(s) | 370, 478 |
| MalI | GATC | 2 cut(s) | 163, 414 |
| MboI | GATC | 2 cut(s) | 161, 412 |
| MboII | GAAGA | 5 cut(s) | 85, 97, 265, 454, 727 |
| MluCI | AATT | 2 cut(s) | 79, 489 |
| MnlI | CCTC | 3 cut(s) | 106, 317, 571 |
| MroXI | GAANNNNTTC | 1 cut(s) | 620 |
| MseI | TTAA | 2 cut(s) | 426, 540 |
| MslI | CAYNNNNRTG | 1 cut(s) | 646 |
| MspI | CCGG | 2 cut(s) | 180, 713 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 350, 359 |
| NcoI | CCATGG | 1 cut(s) | 44 |
| NdeII | GATC | 2 cut(s) | 161, 412 |
| NheI | GCTAGC | 1 cut(s) | 653 |
| NlaIII | CATG | 5 cut(s) | 48, 225, 628, 682, 757 |
| NlaIV | GGNNCC | 3 cut(s) | 22, 134, 570 |
| PceI | AGGCCT | 1 cut(s) | 36 |
| PdmI | GAANNNNTTC | 1 cut(s) | 620 |
| PfeI | GAWTC | 3 cut(s) | 92, 747, 782 |
| PkrI | GCNGC | 3 cut(s) | 203, 346, 364 |
| PspEI | GGTNACC | 1 cut(s) | 370 |
| PspN4I | GGNNCC | 3 cut(s) | 22, 134, 570 |
| PspPI | GGNCC | 3 cut(s) | 41, 568, 728 |
| RsaI | GTAC | 3 cut(s) | 22, 595, 634 |
| RsaNI | GTAC | 3 cut(s) | 21, 594, 633 |
| RseI | CAYNNNNRTG | 1 cut(s) | 646 |
| SaqAI | TTAA | 2 cut(s) | 426, 540 |
| SatI | GCNGC | 3 cut(s) | 202, 345, 363 |
| Sau3AI | GATC | 2 cut(s) | 161, 412 |
| Sau96I | GGNCC | 3 cut(s) | 41, 568, 728 |
| ScaI | AGTACT | 1 cut(s) | 595 |
| SfaNI | GCATC | 2 cut(s) | 200, 397 |
| SinI | GGWCC | 1 cut(s) | 728 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 646 |
| SpeI | ACTAGT | 2 cut(s) | 184, 245 |
| Sse9I | AATT | 2 cut(s) | 79, 489 |
| SseBI | AGGCCT | 1 cut(s) | 36 |
| SsiI | CCGC | 1 cut(s) | 630 |
| SspMI | CTAG | 3 cut(s) | 185, 246, 654 |
| StuI | AGGCCT | 1 cut(s) | 36 |
| StyI | CCWWGG | 3 cut(s) | 37, 44, 235 |
| TaqI | TCGA | 1 cut(s) | 194 |
| TasI | AATT | 2 cut(s) | 79, 489 |
| TatI | WGTACW | 1 cut(s) | 593 |
| TfiI | GAWTC | 3 cut(s) | 92, 747, 782 |
| Tru1I | TTAA | 2 cut(s) | 426, 540 |
| Tru9I | TTAA | 2 cut(s) | 426, 540 |
| TseI | GCWGC | 3 cut(s) | 201, 344, 362 |
| TspDTI | ATGAA | 4 cut(s) | 105, 266, 613, 770 |
| TspGWI | ACGGA | 2 cut(s) | 664, 701 |
| VpaK11BI | GGWCC | 1 cut(s) | 728 |
| XmiI | GTMKAC | 1 cut(s) | 114 |
| XmnI | GAANNNNTTC | 1 cut(s) | 620 |
| XspI | CTAG | 3 cut(s) | 185, 246, 654 |
| ZrmI | AGTACT | 1 cut(s) | 595 |