Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 454, 1082 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 238 |
| AccI | GTMKAC | 1 cut(s) | 36 |
| AciI | CCGC | 1 cut(s) | 771 |
| AclWI | GGATC | 2 cut(s) | 145, 552 |
| AcoI | YGGCCR | 1 cut(s) | 929 |
| AcsI | RAATTY | 4 cut(s) | 107, 379, 444, 916 |
| AcuI | CTGAAG | 4 cut(s) | 363, 678, 777, 1005 |
| AcyI | GRCGYC | 2 cut(s) | 451, 1079 |
| AfaI | GTAC | 1 cut(s) | 1121 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 238, 467, 678, 989 |
| AgsI | TTSAA | 6 cut(s) | 15, 100, 127, 210, 754, 1115 |
| AjnI | CCWGG | 1 cut(s) | 198 |
| AluBI | AGCT | 3 cut(s) | 275, 475, 656 |
| AluI | AGCT | 3 cut(s) | 275, 475, 656 |
| Alw21I | GWGCWC | 2 cut(s) | 123, 395 |
| Alw26I | GTCTC | 2 cut(s) | 76, 1025 |
| AlwI | GGATC | 2 cut(s) | 145, 552 |
| Ama87I | CYCGRG | 2 cut(s) | 75, 1022 |
| AoxI | GGCC | 4 cut(s) | 293, 315, 929, 1008 |
| ApoI | RAATTY | 4 cut(s) | 107, 379, 444, 916 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 485 |
| AspS9I | GGNCC | 1 cut(s) | 891 |
| AsuHPI | GGTGA | 4 cut(s) | 188, 785, 860, 933 |
| AvaI | CYCGRG | 2 cut(s) | 75, 1022 |
| AvaII | GGWCC | 1 cut(s) | 891 |
| BaeGI | GKGCMC | 1 cut(s) | 53 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 570, 603 |
| Bbv12I | GWGCWC | 2 cut(s) | 123, 395 |
| BccI | CCATC | 7 cut(s) | 71, 163, 236, 761, 940, 946, 1141 |
| BceAI | ACGGC | 1 cut(s) | 280 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 168, 202 |
| BciT130I | CCWGG | 1 cut(s) | 200 |
| BclI | TGATCA | 1 cut(s) | 331 |
| BcoDI | GTCTC | 2 cut(s) | 76, 1025 |
| BfaI | CTAG | 3 cut(s) | 467, 636, 861 |
| BfmI | CTRYAG | 1 cut(s) | 582 |
| BlpI | GCTNAGC | 1 cut(s) | 58 |
| Bme1390I | CCNGG | 1 cut(s) | 200 |
| Bme18I | GGWCC | 1 cut(s) | 891 |
| BmeT110I | CYCGRG | 2 cut(s) | 75, 1022 |
| BmgT120I | GGNCC | 1 cut(s) | 891 |
| BmrFI | CCNGG | 1 cut(s) | 200 |
| BmsI | GCATC | 2 cut(s) | 641, 1119 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 58 |
| BpuEI | CTTGAG | 1 cut(s) | 871 |
| Bsa29I | ATCGAT | 1 cut(s) | 66 |
| BsaHI | GRCGYC | 2 cut(s) | 451, 1079 |
| BsaJI | CCNNGG | 1 cut(s) | 926 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 18, 48 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 238, 467, 678, 989 |
| Bse1I | ACTGG | 1 cut(s) | 556 |
| Bse3DI | GCAATG | 1 cut(s) | 12 |
| BseBI | CCWGG | 1 cut(s) | 200 |
| BseCI | ATCGAT | 1 cut(s) | 66 |
| BseDI | CCNNGG | 1 cut(s) | 926 |
| BseGI | GGATG | 3 cut(s) | 247, 361, 772 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 238, 467, 678, 989 |
| BseMI | GCAATG | 1 cut(s) | 12 |
| BseMII | CTCAG | 1 cut(s) | 772 |
| BseNI | ACTGG | 1 cut(s) | 556 |
| BseRI | GAGGAG | 1 cut(s) | 694 |
| BseSI | GKGCMC | 1 cut(s) | 53 |
| BsgI | GTGCAG | 1 cut(s) | 147 |
| BshFI | GGCC | 4 cut(s) | 295, 317, 931, 1010 |
| BshVI | ATCGAT | 1 cut(s) | 66 |
| BsiHKAI | GWGCWC | 2 cut(s) | 123, 395 |
| BsiHKCI | CYCGRG | 2 cut(s) | 75, 1022 |
| BslFI | GGGAC | 1 cut(s) | 107 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 238, 467, 678, 989 |
| BsmAI | GTCTC | 2 cut(s) | 76, 1025 |
| BsmFI | GGGAC | 1 cut(s) | 107 |
| BsnI | GGCC | 4 cut(s) | 295, 317, 931, 1010 |
| BsoBI | CYCGRG | 2 cut(s) | 75, 1022 |
| Bsp1286I | GDGCHC | 3 cut(s) | 53, 123, 395 |
| Bsp1407I | TGTACA | 1 cut(s) | 1119 |
| Bsp143I | GATC | 3 cut(s) | 150, 331, 544 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 58 |
| BspACI | CCGC | 1 cut(s) | 771 |
| BspANI | GGCC | 4 cut(s) | 295, 317, 931, 1010 |
| BspCNI | CTCAG | 1 cut(s) | 771 |
| BspDI | ATCGAT | 1 cut(s) | 66 |
| BspMAI | CTGCAG | 1 cut(s) | 586 |
| BspPI | GGATC | 2 cut(s) | 145, 552 |
| BsrDI | GCAATG | 1 cut(s) | 12 |
| BsrGI | TGTACA | 1 cut(s) | 1119 |
| BsrI | ACTGG | 1 cut(s) | 556 |
| BssECI | CCNNGG | 1 cut(s) | 926 |
| BssMI | GATC | 3 cut(s) | 150, 331, 544 |
| BssNI | GRCGYC | 2 cut(s) | 451, 1079 |
| Bst2UI | CCWGG | 1 cut(s) | 200 |
| Bst4CI | ACNGT | 2 cut(s) | 422, 697 |
| Bst6I | CTCTTC | 1 cut(s) | 503 |
| BstACI | GRCGYC | 2 cut(s) | 451, 1079 |
| BstAUI | TGTACA | 1 cut(s) | 1119 |
| BstC8I | GCNNGC | 2 cut(s) | 534, 654 |
| BstDEI | CTNAG | 4 cut(s) | 58, 399, 758, 1125 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 465 |
| BstF5I | GGATG | 3 cut(s) | 247, 361, 772 |
| BstKTI | GATC | 3 cut(s) | 153, 334, 547 |
| BstMAI | GTCTC | 2 cut(s) | 76, 1025 |
| BstMBI | GATC | 3 cut(s) | 150, 331, 544 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 301 |
| BstNI | CCWGG | 1 cut(s) | 200 |
| BstSCI | CCNGG | 1 cut(s) | 198 |
| BstSFI | CTRYAG | 1 cut(s) | 582 |
| BstSLI | GKGCMC | 1 cut(s) | 53 |
| BstX2I | RGATCY | 1 cut(s) | 150 |
| BstYI | RGATCY | 1 cut(s) | 150 |
| Bsu15I | ATCGAT | 1 cut(s) | 66 |
| BsuRI | GGCC | 4 cut(s) | 295, 317, 931, 1010 |
| BsuTUI | ATCGAT | 1 cut(s) | 66 |
| BtgZI | GCGATG | 1 cut(s) | 918 |
| BtsCI | GGATG | 3 cut(s) | 247, 361, 772 |
| BtsI | GCAGTG | 1 cut(s) | 393 |
| BtsIMutI | CAGTG | 4 cut(s) | 164, 393, 693, 766 |
| Cac8I | GCNNGC | 2 cut(s) | 534, 654 |
| Cfr13I | GGNCC | 1 cut(s) | 891 |
| ClaI | ATCGAT | 1 cut(s) | 66 |
| CseI | GACGC | 1 cut(s) | 986 |
| Csp6I | GTAC | 1 cut(s) | 1120 |
| CviAII | CATG | 6 cut(s) | 186, 249, 260, 335, 974, 1006 |
| CviQI | GTAC | 1 cut(s) | 1120 |
| DdeI | CTNAG | 4 cut(s) | 58, 399, 758, 1125 |
| DpnI | GATC | 3 cut(s) | 152, 333, 546 |
| DpnII | GATC | 3 cut(s) | 150, 331, 544 |
| EaeI | YGGCCR | 1 cut(s) | 929 |
| Eam1104I | CTCTTC | 1 cut(s) | 503 |
| EarI | CTCTTC | 1 cut(s) | 503 |
| Eco32I | GATATC | 1 cut(s) | 82 |
| Eco47I | GGWCC | 1 cut(s) | 891 |
| Eco57I | CTGAAG | 4 cut(s) | 363, 678, 777, 1005 |
| Eco88I | CYCGRG | 2 cut(s) | 75, 1022 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 465 |
| EcoO109I | RGGNCCY | 1 cut(s) | 891 |
| EcoRI | GAATTC | 1 cut(s) | 444 |
| EcoRII | CCWGG | 1 cut(s) | 198 |
| EcoRV | GATATC | 1 cut(s) | 82 |
| FaeI | CATG | 6 cut(s) | 189, 252, 263, 338, 977, 1009 |
| FaqI | GGGAC | 1 cut(s) | 107 |
| FatI | CATG | 6 cut(s) | 185, 248, 259, 334, 973, 1005 |
| FbaI | TGATCA | 1 cut(s) | 331 |
| FblI | GTMKAC | 1 cut(s) | 36 |
| FokI | GGATG | 3 cut(s) | 254, 348, 779 |
| FspBI | CTAG | 3 cut(s) | 467, 636, 861 |
| HaeIII | GGCC | 4 cut(s) | 295, 317, 931, 1010 |
| HgaI | GACGC | 1 cut(s) | 986 |
| Hin1I | GRCGYC | 2 cut(s) | 451, 1079 |
| Hin1II | CATG | 6 cut(s) | 189, 252, 263, 338, 977, 1009 |
| HincII | GTYRAC | 1 cut(s) | 455 |
| HindII | GTYRAC | 1 cut(s) | 455 |
| HindIII | AAGCTT | 1 cut(s) | 473 |
| HinfI | GANTC | 2 cut(s) | 206, 754 |
| HphI | GGTGA | 4 cut(s) | 188, 785, 860, 933 |
| Hpy166II | GTNNAC | 5 cut(s) | 37, 455, 693, 1101, 1122 |
| Hpy188I | TCNGA | 2 cut(s) | 343, 709 |
| Hpy188III | TCNNGA | 8 cut(s) | 77, 100, 144, 232, 374, 620, 804, 1024 |
| Hpy8I | GTNNAC | 5 cut(s) | 37, 455, 693, 1101, 1122 |
| Hpy99I | CGWCG | 1 cut(s) | 453 |
| HpyAV | CCTTC | 2 cut(s) | 338, 375 |
| HpyCH4III | ACNGT | 2 cut(s) | 422, 697 |
| HpyCH4IV | ACGT | 2 cut(s) | 451, 1079 |
| HpyCH4V | TGCA | 8 cut(s) | 22, 164, 263, 304, 584, 595, 632, 1132 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 301 |
| HpyF3I | CTNAG | 4 cut(s) | 58, 399, 758, 1125 |
| HpySE526I | ACGT | 2 cut(s) | 451, 1079 |
| Hsp92I | GRCGYC | 2 cut(s) | 451, 1079 |
| Hsp92II | CATG | 6 cut(s) | 189, 252, 263, 338, 977, 1009 |
| Ksp22I | TGATCA | 1 cut(s) | 331 |
| Kzo9I | GATC | 3 cut(s) | 150, 331, 544 |
| LweI | GCATC | 2 cut(s) | 641, 1119 |
| MaeI | CTAG | 3 cut(s) | 467, 636, 861 |
| MaeII | ACGT | 2 cut(s) | 451, 1079 |
| MaeIII | GTNAC | 5 cut(s) | 697, 796, 871, 921, 1075 |
| MalI | GATC | 3 cut(s) | 152, 333, 546 |
| MboI | GATC | 3 cut(s) | 150, 331, 544 |
| MboII | GAAGA | 7 cut(s) | 248, 520, 525, 671, 972, 975, 1103 |
| MflI | RGATCY | 1 cut(s) | 150 |
| MhlI | GDGCHC | 3 cut(s) | 53, 123, 395 |
| MluCI | AATT | 5 cut(s) | 107, 379, 444, 916, 1107 |
| MmeI | TCCRAC | 1 cut(s) | 970 |
| MroXI | GAANNNNTTC | 1 cut(s) | 485 |
| MseI | TTAA | 1 cut(s) | 1050 |
| MslI | CAYNNNNRTG | 2 cut(s) | 600, 1127 |
| MspA1I | CMGCKG | 1 cut(s) | 656 |
| MspR9I | CCNGG | 1 cut(s) | 200 |
| MvaI | CCWGG | 1 cut(s) | 200 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 301 |
| NdeII | GATC | 3 cut(s) | 150, 331, 544 |
| NlaIII | CATG | 6 cut(s) | 189, 252, 263, 338, 977, 1009 |
| NmeAIII | GCCGAG | 1 cut(s) | 907 |
| NmuCI | GTSAC | 2 cut(s) | 697, 1075 |
| PdmI | GAANNNNTTC | 1 cut(s) | 485 |
| PfeI | GAWTC | 2 cut(s) | 206, 754 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 238 |
| PpuMI | RGGWCCY | 1 cut(s) | 891 |
| Psp5II | RGGWCCY | 1 cut(s) | 891 |
| Psp6I | CCWGG | 1 cut(s) | 198 |
| PspGI | CCWGG | 1 cut(s) | 198 |
| PspPI | GGNCC | 1 cut(s) | 891 |
| PspPPI | RGGWCCY | 1 cut(s) | 891 |
| PstI | CTGCAG | 1 cut(s) | 586 |
| PsuI | RGATCY | 1 cut(s) | 150 |
| PvuII | CAGCTG | 1 cut(s) | 656 |
| RsaI | GTAC | 1 cut(s) | 1121 |
| RsaNI | GTAC | 1 cut(s) | 1120 |
| RseI | CAYNNNNRTG | 2 cut(s) | 600, 1127 |
| SaqAI | TTAA | 1 cut(s) | 1050 |
| Sau3AI | GATC | 3 cut(s) | 150, 331, 544 |
| Sau96I | GGNCC | 1 cut(s) | 891 |
| ScrFI | CCNGG | 1 cut(s) | 200 |
| SduI | GDGCHC | 3 cut(s) | 53, 123, 395 |
| SfaNI | GCATC | 2 cut(s) | 641, 1119 |
| SfcI | CTRYAG | 1 cut(s) | 582 |
| SinI | GGWCC | 1 cut(s) | 891 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 600, 1127 |
| SmlI | CTYRAG | 1 cut(s) | 886 |
| SmoI | CTYRAG | 1 cut(s) | 886 |
| Sse9I | AATT | 5 cut(s) | 107, 379, 444, 916, 1107 |
| SsiI | CCGC | 1 cut(s) | 771 |
| SspI | AATATT | 1 cut(s) | 523 |
| SspMI | CTAG | 3 cut(s) | 467, 636, 861 |
| StyD4I | CCNGG | 1 cut(s) | 198 |
| TaaI | ACNGT | 2 cut(s) | 422, 697 |
| TaiI | ACGT | 2 cut(s) | 454, 1082 |
| TaqI | TCGA | 3 cut(s) | 66, 448, 677 |
| TasI | AATT | 5 cut(s) | 107, 379, 444, 916, 1107 |
| TatI | WGTACW | 1 cut(s) | 1119 |
| TfiI | GAWTC | 2 cut(s) | 206, 754 |
| Tru1I | TTAA | 1 cut(s) | 1050 |
| Tru9I | TTAA | 1 cut(s) | 1050 |
| TscAI | CASTG | 4 cut(s) | 171, 400, 700, 766 |
| TseFI | GTSAC | 2 cut(s) | 697, 1075 |
| Tsp45I | GTSAC | 2 cut(s) | 697, 1075 |
| TspDTI | ATGAA | 4 cut(s) | 248, 515, 693, 800 |
| TspRI | CASTG | 4 cut(s) | 171, 400, 700, 766 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 238 |
| VpaK11BI | GGWCC | 1 cut(s) | 891 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 465 |
| XapI | RAATTY | 4 cut(s) | 107, 379, 444, 916 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 239 |
| XmiI | GTMKAC | 1 cut(s) | 36 |
| XmnI | GAANNNNTTC | 1 cut(s) | 485 |
| XspI | CTAG | 3 cut(s) | 467, 636, 861 |
| ZraI | GACGTC | 2 cut(s) | 452, 1080 |