Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 680 |
| AccII | CGCG | 1 cut(s) | 462 |
| AciI | CCGC | 1 cut(s) | 112 |
| AclI | AACGTT | 1 cut(s) | 66 |
| AcoI | YGGCCR | 2 cut(s) | 58, 256 |
| AcsI | RAATTY | 3 cut(s) | 339, 396, 452 |
| AcuI | CTGAAG | 1 cut(s) | 720 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 737 |
| AflII | CTTAAG | 1 cut(s) | 184 |
| AgsI | TTSAA | 5 cut(s) | 304, 367, 473, 509, 515 |
| AhlI | ACTAGT | 1 cut(s) | 812 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 825, 857 |
| AluBI | AGCT | 4 cut(s) | 97, 254, 644, 783 |
| AluI | AGCT | 4 cut(s) | 97, 254, 644, 783 |
| Alw26I | GTCTC | 1 cut(s) | 730 |
| AoxI | GGCC | 3 cut(s) | 58, 256, 764 |
| ApeKI | GCWGC | 3 cut(s) | 573, 644, 818 |
| ApoI | RAATTY | 3 cut(s) | 339, 396, 452 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 84, 308 |
| AspS9I | GGNCC | 2 cut(s) | 239, 764 |
| AsuHPI | GGTGA | 2 cut(s) | 416, 752 |
| AsuNHI | GCTAGC | 1 cut(s) | 647 |
| AvaII | GGWCC | 1 cut(s) | 239 |
| BalI | TGGCCA | 2 cut(s) | 60, 258 |
| BbvI | GCAGC | 3 cut(s) | 560, 631, 830 |
| BclI | TGATCA | 3 cut(s) | 295, 634, 696 |
| BcoDI | GTCTC | 1 cut(s) | 730 |
| BcuI | ACTAGT | 1 cut(s) | 812 |
| BfaI | CTAG | 4 cut(s) | 444, 648, 813, 845 |
| BfmI | CTRYAG | 1 cut(s) | 784 |
| BfrI | CTTAAG | 1 cut(s) | 184 |
| BisI | GCNGC | 3 cut(s) | 574, 645, 819 |
| BlsI | GCNGC | 3 cut(s) | 575, 646, 820 |
| Bme18I | GGWCC | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 2 cut(s) | 239, 764 |
| BmiI | GGNNCC | 2 cut(s) | 240, 765 |
| BmsI | GCATC | 2 cut(s) | 555, 762 |
| BmtI | GCTAGC | 1 cut(s) | 651 |
| BoxI | GACNNNNGTC | 1 cut(s) | 597 |
| Bpu10I | CCTNAGC | 1 cut(s) | 588 |
| BpuEI | CTTGAG | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 2 cut(s) | 242, 767 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 737 |
| BseDI | CCNNGG | 2 cut(s) | 242, 767 |
| BseGI | GGATG | 1 cut(s) | 777 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 737 |
| BseMII | CTCAG | 2 cut(s) | 167, 884 |
| BseRI | GAGGAG | 1 cut(s) | 720 |
| BseXI | GCAGC | 3 cut(s) | 560, 631, 830 |
| BsgI | GTGCAG | 1 cut(s) | 837 |
| Bsh1236I | CGCG | 1 cut(s) | 462 |
| BshFI | GGCC | 3 cut(s) | 60, 258, 766 |
| BslFI | GGGAC | 2 cut(s) | 252, 585 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 737 |
| BsmAI | GTCTC | 1 cut(s) | 730 |
| BsmFI | GGGAC | 2 cut(s) | 252, 585 |
| BsnI | GGCC | 3 cut(s) | 60, 258, 766 |
| Bsp143I | GATC | 3 cut(s) | 295, 634, 696 |
| BspACI | CCGC | 1 cut(s) | 112 |
| BspANI | GGCC | 3 cut(s) | 60, 258, 766 |
| BspCNI | CTCAG | 2 cut(s) | 168, 885 |
| BspFNI | CGCG | 1 cut(s) | 462 |
| BspHI | TCATGA | 1 cut(s) | 699 |
| BspLI | GGNNCC | 2 cut(s) | 240, 765 |
| BspOI | GCTAGC | 1 cut(s) | 651 |
| BspTI | CTTAAG | 1 cut(s) | 184 |
| BssECI | CCNNGG | 2 cut(s) | 242, 767 |
| BssMI | GATC | 3 cut(s) | 295, 634, 696 |
| BssT1I | CCWWGG | 2 cut(s) | 242, 767 |
| Bst4CI | ACNGT | 3 cut(s) | 35, 379, 677 |
| BstAFI | CTTAAG | 1 cut(s) | 184 |
| BstC8I | GCNNGC | 4 cut(s) | 256, 460, 649, 653 |
| BstDEI | CTNAG | 3 cut(s) | 176, 588, 893 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 735 |
| BstF5I | GGATG | 1 cut(s) | 777 |
| BstFNI | CGCG | 1 cut(s) | 462 |
| BstKTI | GATC | 3 cut(s) | 298, 637, 699 |
| BstMAI | GTCTC | 1 cut(s) | 730 |
| BstMBI | GATC | 3 cut(s) | 295, 634, 696 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 772 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 597 |
| BstSFI | CTRYAG | 1 cut(s) | 784 |
| BstUI | CGCG | 1 cut(s) | 462 |
| BstV1I | GCAGC | 3 cut(s) | 560, 631, 830 |
| BsuRI | GGCC | 3 cut(s) | 60, 258, 766 |
| BtsCI | GGATG | 1 cut(s) | 777 |
| BtsI | GCAGTG | 1 cut(s) | 491 |
| BtsIMutI | CAGTG | 2 cut(s) | 491, 682 |
| Cac8I | GCNNGC | 4 cut(s) | 256, 460, 649, 653 |
| CciI | TCATGA | 1 cut(s) | 699 |
| Cfr13I | GGNCC | 2 cut(s) | 239, 764 |
| CseI | GACGC | 2 cut(s) | 451, 485 |
| CviAII | CATG | 4 cut(s) | 56, 584, 700, 876 |
| DdeI | CTNAG | 3 cut(s) | 176, 588, 893 |
| DpnI | GATC | 3 cut(s) | 297, 636, 698 |
| DpnII | GATC | 3 cut(s) | 295, 634, 696 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 680 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 680 |
| EaeI | YGGCCR | 2 cut(s) | 58, 256 |
| Eco130I | CCWWGG | 2 cut(s) | 242, 767 |
| Eco47I | GGWCC | 1 cut(s) | 239 |
| Eco57I | CTGAAG | 1 cut(s) | 720 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 735 |
| EcoO109I | RGGNCCY | 2 cut(s) | 239, 764 |
| EcoT14I | CCWWGG | 2 cut(s) | 242, 767 |
| ErhI | CCWWGG | 2 cut(s) | 242, 767 |
| FaeI | CATG | 4 cut(s) | 59, 587, 703, 879 |
| FaqI | GGGAC | 2 cut(s) | 252, 585 |
| FatI | CATG | 4 cut(s) | 55, 583, 699, 875 |
| FauNDI | CATATG | 1 cut(s) | 502 |
| FbaI | TGATCA | 3 cut(s) | 295, 634, 696 |
| Fnu4HI | GCNGC | 3 cut(s) | 574, 645, 819 |
| FokI | GGATG | 1 cut(s) | 784 |
| Fsp4HI | GCNGC | 3 cut(s) | 574, 645, 819 |
| FspBI | CTAG | 4 cut(s) | 444, 648, 813, 845 |
| GluI | GCNGC | 3 cut(s) | 574, 645, 819 |
| HaeIII | GGCC | 3 cut(s) | 60, 258, 766 |
| HgaI | GACGC | 2 cut(s) | 451, 485 |
| Hin1II | CATG | 4 cut(s) | 59, 587, 703, 879 |
| HincII | GTYRAC | 1 cut(s) | 421 |
| HindII | GTYRAC | 1 cut(s) | 421 |
| HindIII | AAGCTT | 1 cut(s) | 95 |
| HinfI | GANTC | 2 cut(s) | 515, 880 |
| HphI | GGTGA | 2 cut(s) | 416, 752 |
| Hpy166II | GTNNAC | 3 cut(s) | 21, 375, 421 |
| Hpy188I | TCNGA | 4 cut(s) | 79, 338, 673, 885 |
| Hpy188III | TCNNGA | 3 cut(s) | 444, 700, 845 |
| Hpy8I | GTNNAC | 3 cut(s) | 21, 375, 421 |
| HpyAV | CCTTC | 5 cut(s) | 82, 183, 409, 536, 744 |
| HpyCH4III | ACNGT | 3 cut(s) | 35, 379, 677 |
| HpyCH4IV | ACGT | 1 cut(s) | 66 |
| HpyCH4V | TGCA | 4 cut(s) | 524, 620, 818, 861 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 772 |
| HpyF3I | CTNAG | 3 cut(s) | 176, 588, 893 |
| HpySE526I | ACGT | 1 cut(s) | 66 |
| Hsp92II | CATG | 4 cut(s) | 59, 587, 703, 879 |
| Ksp22I | TGATCA | 3 cut(s) | 295, 634, 696 |
| Kzo9I | GATC | 3 cut(s) | 295, 634, 696 |
| LpnPI | CCDG | 9 cut(s) | 74, 240, 272, 300, 366, 630, 665, 723, 824 |
| Lsp1109I | GCAGC | 3 cut(s) | 560, 631, 830 |
| LweI | GCATC | 2 cut(s) | 555, 762 |
| MaeI | CTAG | 4 cut(s) | 444, 648, 813, 845 |
| MaeII | ACGT | 1 cut(s) | 66 |
| MalI | GATC | 3 cut(s) | 297, 636, 698 |
| MboI | GATC | 3 cut(s) | 295, 634, 696 |
| MboII | GAAGA | 3 cut(s) | 221, 224, 767 |
| MlsI | TGGCCA | 2 cut(s) | 60, 258 |
| MluCI | AATT | 6 cut(s) | 148, 339, 387, 396, 452, 474 |
| MluNI | TGGCCA | 2 cut(s) | 60, 258 |
| MnlI | CCTC | 5 cut(s) | 173, 267, 440, 666, 741 |
| Mox20I | TGGCCA | 2 cut(s) | 60, 258 |
| MroXI | GAANNNNTTC | 2 cut(s) | 84, 308 |
| MscI | TGGCCA | 2 cut(s) | 60, 258 |
| MseI | TTAA | 4 cut(s) | 129, 185, 204, 750 |
| MslI | CAYNNNNRTG | 1 cut(s) | 303 |
| Msp20I | TGGCCA | 2 cut(s) | 60, 258 |
| MspCI | CTTAAG | 1 cut(s) | 184 |
| MvnI | CGCG | 1 cut(s) | 462 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 772 |
| NdeI | CATATG | 1 cut(s) | 502 |
| NdeII | GATC | 3 cut(s) | 295, 634, 696 |
| NheI | GCTAGC | 1 cut(s) | 647 |
| NlaIII | CATG | 4 cut(s) | 59, 587, 703, 879 |
| NlaIV | GGNNCC | 2 cut(s) | 240, 765 |
| PagI | TCATGA | 1 cut(s) | 699 |
| PdmI | GAANNNNTTC | 2 cut(s) | 84, 308 |
| PfeI | GAWTC | 2 cut(s) | 515, 880 |
| PkrI | GCNGC | 3 cut(s) | 575, 646, 820 |
| PpuMI | RGGWCCY | 1 cut(s) | 239 |
| PshAI | GACNNNNGTC | 1 cut(s) | 597 |
| Psp1406I | AACGTT | 1 cut(s) | 66 |
| Psp5II | RGGWCCY | 1 cut(s) | 239 |
| PspN4I | GGNNCC | 2 cut(s) | 240, 765 |
| PspPI | GGNCC | 2 cut(s) | 239, 764 |
| PspPPI | RGGWCCY | 1 cut(s) | 239 |
| RseI | CAYNNNNRTG | 1 cut(s) | 303 |
| SaqAI | TTAA | 4 cut(s) | 129, 185, 204, 750 |
| SatI | GCNGC | 3 cut(s) | 574, 645, 819 |
| Sau3AI | GATC | 3 cut(s) | 295, 634, 696 |
| Sau96I | GGNCC | 2 cut(s) | 239, 764 |
| SfaNI | GCATC | 2 cut(s) | 555, 762 |
| SfcI | CTRYAG | 1 cut(s) | 784 |
| SinI | GGWCC | 1 cut(s) | 239 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 303 |
| SmlI | CTYRAG | 2 cut(s) | 45, 184 |
| SmoI | CTYRAG | 2 cut(s) | 45, 184 |
| SpeI | ACTAGT | 1 cut(s) | 812 |
| Sse9I | AATT | 6 cut(s) | 148, 339, 387, 396, 452, 474 |
| SsiI | CCGC | 1 cut(s) | 112 |
| SspMI | CTAG | 4 cut(s) | 444, 648, 813, 845 |
| StyI | CCWWGG | 2 cut(s) | 242, 767 |
| TaaI | ACNGT | 3 cut(s) | 35, 379, 677 |
| TaiI | ACGT | 1 cut(s) | 69 |
| TasI | AATT | 6 cut(s) | 148, 339, 387, 396, 452, 474 |
| TfiI | GAWTC | 2 cut(s) | 515, 880 |
| Tru1I | TTAA | 4 cut(s) | 129, 185, 204, 750 |
| Tru9I | TTAA | 4 cut(s) | 129, 185, 204, 750 |
| TscAI | CASTG | 2 cut(s) | 498, 682 |
| TseI | GCWGC | 3 cut(s) | 573, 644, 818 |
| TspDTI | ATGAA | 5 cut(s) | 27, 77, 233, 301, 555 |
| TspGWI | ACGGA | 1 cut(s) | 20 |
| TspRI | CASTG | 2 cut(s) | 498, 682 |
| Vha464I | CTTAAG | 1 cut(s) | 184 |
| VpaK11BI | GGWCC | 1 cut(s) | 239 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 735 |
| XapI | RAATTY | 3 cut(s) | 339, 396, 452 |
| XbaI | TCTAGA | 2 cut(s) | 443, 844 |
| XmnI | GAANNNNTTC | 2 cut(s) | 84, 308 |
| XspI | CTAG | 4 cut(s) | 444, 648, 813, 845 |