Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 102, 188 |
| AclI | AACGTT | 2 cut(s) | 585, 1502 |
| AclWI | GGATC | 5 cut(s) | 201, 1156, 1201, 1535, 1611 |
| AcoI | YGGCCR | 1 cut(s) | 487 |
| AcsI | RAATTY | 4 cut(s) | 8, 693, 919, 994 |
| AfaI | GTAC | 5 cut(s) | 79, 754, 906, 928, 1069 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 59, 370, 439, 1091 |
| AflII | CTTAAG | 1 cut(s) | 377 |
| AgsI | TTSAA | 9 cut(s) | 590, 704, 746, 797, 985, 1105, 1412, 1448, 1685 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 121 |
| AhlI | ACTAGT | 1 cut(s) | 1166 |
| AjnI | CCWGG | 3 cut(s) | 81, 517, 855 |
| AleI | CACNNNNGTG | 1 cut(s) | 1271 |
| Alw21I | GWGCWC | 3 cut(s) | 76, 278, 462 |
| Alw26I | GTCTC | 1 cut(s) | 129 |
| AlwI | GGATC | 5 cut(s) | 201, 1156, 1201, 1535, 1611 |
| AoxI | GGCC | 6 cut(s) | 49, 287, 333, 487, 748, 1361 |
| ApeKI | GCWGC | 1 cut(s) | 1423 |
| ApoI | RAATTY | 4 cut(s) | 8, 693, 919, 994 |
| AseI | ATTAAT | 3 cut(s) | 573, 780, 1569 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 594 |
| AspS9I | GGNCC | 3 cut(s) | 50, 383, 470 |
| AsuHPI | GGTGA | 3 cut(s) | 74, 538, 1249 |
| AsuII | TTCGAA | 1 cut(s) | 568 |
| AvaII | GGWCC | 2 cut(s) | 383, 470 |
| BaeGI | GKGCMC | 1 cut(s) | 376 |
| BalI | TGGCCA | 1 cut(s) | 489 |
| BanII | GRGCYC | 3 cut(s) | 76, 278, 1439 |
| BauI | CACGAG | 2 cut(s) | 675, 1254 |
| Bbv12I | GWGCWC | 3 cut(s) | 76, 278, 462 |
| BbvCI | CCTCAGC | 1 cut(s) | 945 |
| BbvI | GCAGC | 1 cut(s) | 1410 |
| BccI | CCATC | 4 cut(s) | 41, 363, 1555, 1670 |
| BceAI | ACGGC | 3 cut(s) | 106, 735, 1673 |
| BciT130I | CCWGG | 3 cut(s) | 83, 519, 857 |
| BcoDI | GTCTC | 1 cut(s) | 129 |
| BcuI | ACTAGT | 1 cut(s) | 1166 |
| BfaI | CTAG | 4 cut(s) | 404, 1167, 1176, 1323 |
| BfrI | CTTAAG | 1 cut(s) | 377 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1694 |
| BglII | AGATCT | 2 cut(s) | 350, 400 |
| BisI | GCNGC | 1 cut(s) | 1424 |
| BlpI | GCTNAGC | 4 cut(s) | 717, 1035, 1040, 1698 |
| BlsI | GCNGC | 1 cut(s) | 1425 |
| Bme1390I | CCNGG | 3 cut(s) | 83, 519, 857 |
| Bme18I | GGWCC | 2 cut(s) | 383, 470 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 121 |
| BmgT120I | GGNCC | 3 cut(s) | 50, 383, 470 |
| BmiI | GGNNCC | 4 cut(s) | 37, 384, 861, 951 |
| BmrFI | CCNGG | 3 cut(s) | 83, 519, 857 |
| BmsI | GCATC | 2 cut(s) | 643, 951 |
| Bpu10I | CCTNAGC | 1 cut(s) | 945 |
| Bpu1102I | GCTNAGC | 4 cut(s) | 717, 1035, 1040, 1698 |
| Bpu14I | TTCGAA | 1 cut(s) | 568 |
| BpuEI | CTTGAG | 1 cut(s) | 1274 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1602 |
| BsaJI | CCNNGG | 6 cut(s) | 81, 290, 473, 989, 1308, 1364 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 182, 212, 962, 992 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 59, 370, 439, 1091 |
| Bse1I | ACTGG | 5 cut(s) | 111, 469, 1069, 1648, 1653 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1602 |
| BseBI | CCWGG | 3 cut(s) | 83, 519, 857 |
| BseDI | CCNNGG | 6 cut(s) | 81, 290, 473, 989, 1308, 1364 |
| BseGI | GGATG | 4 cut(s) | 100, 658, 809, 981 |
| BseJI | GATNNNNATC | 1 cut(s) | 1602 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 59, 370, 439, 1091 |
| BseMII | CTCAG | 8 cut(s) | 273, 356, 361, 679, 936, 1026, 1107, 1689 |
| BseNI | ACTGG | 5 cut(s) | 111, 469, 1069, 1648, 1653 |
| BseRI | GAGGAG | 2 cut(s) | 1133, 1143 |
| BseSI | GKGCMC | 1 cut(s) | 376 |
| BseXI | GCAGC | 1 cut(s) | 1410 |
| BseYI | CCCAGC | 3 cut(s) | 23, 1232, 1379 |
| BshFI | GGCC | 6 cut(s) | 51, 289, 335, 489, 750, 1363 |
| BsiHKAI | GWGCWC | 3 cut(s) | 76, 278, 462 |
| BsiSI | CCGG | 1 cut(s) | 5 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 59, 370, 439, 1091 |
| BsmAI | GTCTC | 1 cut(s) | 129 |
| BsmBI | CGTCTC | 1 cut(s) | 129 |
| BsnI | GGCC | 6 cut(s) | 51, 289, 335, 489, 750, 1363 |
| Bsp119I | TTCGAA | 1 cut(s) | 568 |
| Bsp1286I | GDGCHC | 5 cut(s) | 76, 278, 376, 462, 1439 |
| Bsp1407I | TGTACA | 1 cut(s) | 904 |
| Bsp143I | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| Bsp1720I | GCTNAGC | 4 cut(s) | 717, 1035, 1040, 1698 |
| BspACI | CCGC | 2 cut(s) | 102, 188 |
| BspANI | GGCC | 6 cut(s) | 51, 289, 335, 489, 750, 1363 |
| BspCNI | CTCAG | 8 cut(s) | 272, 357, 360, 678, 937, 1027, 1108, 1690 |
| BspHI | TCATGA | 2 cut(s) | 1402, 1537 |
| BspLI | GGNNCC | 4 cut(s) | 37, 384, 861, 951 |
| BspPI | GGATC | 5 cut(s) | 201, 1156, 1201, 1535, 1611 |
| BspT104I | TTCGAA | 1 cut(s) | 568 |
| BspTI | CTTAAG | 1 cut(s) | 377 |
| BsrGI | TGTACA | 1 cut(s) | 904 |
| BsrI | ACTGG | 5 cut(s) | 111, 469, 1069, 1648, 1653 |
| BssECI | CCNNGG | 6 cut(s) | 81, 290, 473, 989, 1308, 1364 |
| BssMI | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| BssSI | CACGAG | 2 cut(s) | 675, 1254 |
| BssT1I | CCWWGG | 4 cut(s) | 290, 473, 989, 1364 |
| Bst2BI | CACGAG | 2 cut(s) | 675, 1254 |
| Bst2UI | CCWGG | 3 cut(s) | 83, 519, 857 |
| Bst4CI | ACNGT | 1 cut(s) | 904 |
| Bst6I | CTCTTC | 3 cut(s) | 138, 360, 1128 |
| BstAFI | CTTAAG | 1 cut(s) | 377 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1229 |
| BstAUI | TGTACA | 1 cut(s) | 904 |
| BstBI | TTCGAA | 1 cut(s) | 568 |
| BstC8I | GCNNGC | 2 cut(s) | 487, 846 |
| BstDSI | CCRYGG | 1 cut(s) | 1308 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 437 |
| BstF5I | GGATG | 4 cut(s) | 100, 658, 809, 981 |
| BstKTI | GATC | 8 cut(s) | 196, 353, 403, 546, 1151, 1196, 1543, 1606 |
| BstMAI | GTCTC | 1 cut(s) | 129 |
| BstMBI | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1229, 1694 |
| BstNI | CCWGG | 3 cut(s) | 83, 519, 857 |
| BstNSI | RCATGY | 1 cut(s) | 1706 |
| BstSCI | CCNGG | 3 cut(s) | 81, 517, 855 |
| BstSLI | GKGCMC | 1 cut(s) | 376 |
| BstV1I | GCAGC | 1 cut(s) | 1410 |
| BstX2I | RGATCY | 4 cut(s) | 350, 400, 1148, 1193 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1309 |
| BstYI | RGATCY | 4 cut(s) | 350, 400, 1148, 1193 |
| BsuRI | GGCC | 6 cut(s) | 51, 289, 335, 489, 750, 1363 |
| BtgI | CCRYGG | 1 cut(s) | 1308 |
| BtgZI | GCGATG | 1 cut(s) | 225 |
| BtsCI | GGATG | 4 cut(s) | 100, 658, 809, 981 |
| BtsIMutI | CAGTG | 1 cut(s) | 462 |
| Cac8I | GCNNGC | 2 cut(s) | 487, 846 |
| CciI | TCATGA | 2 cut(s) | 1402, 1537 |
| Cfr13I | GGNCC | 3 cut(s) | 50, 383, 470 |
| CsiI | ACCWGGT | 1 cut(s) | 855 |
| Csp6I | GTAC | 5 cut(s) | 78, 753, 905, 927, 1068 |
| CviAII | CATG | 8 cut(s) | 319, 815, 1012, 1269, 1403, 1538, 1673, 1703 |
| CviQI | GTAC | 5 cut(s) | 78, 753, 905, 927, 1068 |
| DpnI | GATC | 8 cut(s) | 195, 352, 402, 545, 1150, 1195, 1542, 1605 |
| DpnII | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| DraI | TTTAAA | 1 cut(s) | 1279 |
| DriI | GACNNNNNGTC | 1 cut(s) | 121 |
| EaeI | YGGCCR | 1 cut(s) | 487 |
| Eam1104I | CTCTTC | 3 cut(s) | 138, 360, 1128 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 121 |
| EarI | CTCTTC | 3 cut(s) | 138, 360, 1128 |
| EciI | GGCGGA | 2 cut(s) | 117, 203 |
| Ecl136II | GAGCTC | 2 cut(s) | 74, 276 |
| Eco130I | CCWWGG | 4 cut(s) | 290, 473, 989, 1364 |
| Eco147I | AGGCCT | 1 cut(s) | 1363 |
| Eco24I | GRGCYC | 3 cut(s) | 76, 278, 1439 |
| Eco32I | GATATC | 1 cut(s) | 1552 |
| Eco47I | GGWCC | 2 cut(s) | 383, 470 |
| Eco53kI | GAGCTC | 2 cut(s) | 74, 276 |
| EcoICRI | GAGCTC | 2 cut(s) | 74, 276 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 437 |
| EcoO109I | RGGNCCY | 1 cut(s) | 383 |
| EcoRII | CCWGG | 3 cut(s) | 81, 517, 855 |
| EcoRV | GATATC | 1 cut(s) | 1552 |
| EcoT14I | CCWWGG | 4 cut(s) | 290, 473, 989, 1364 |
| EcoT38I | GRGCYC | 3 cut(s) | 76, 278, 1439 |
| ErhI | CCWWGG | 4 cut(s) | 290, 473, 989, 1364 |
| Esp3I | CGTCTC | 1 cut(s) | 129 |
| FaeI | CATG | 8 cut(s) | 322, 818, 1015, 1272, 1406, 1541, 1676, 1706 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1348, 1380 |
| FatI | CATG | 8 cut(s) | 318, 814, 1011, 1268, 1402, 1537, 1672, 1702 |
| FauNDI | CATATG | 1 cut(s) | 552 |
| Fnu4HI | GCNGC | 1 cut(s) | 1424 |
| FokI | GGATG | 4 cut(s) | 107, 665, 816, 988 |
| FriOI | GRGCYC | 3 cut(s) | 76, 278, 1439 |
| Fsp4HI | GCNGC | 1 cut(s) | 1424 |
| FspBI | CTAG | 4 cut(s) | 404, 1167, 1176, 1323 |
| GluI | GCNGC | 1 cut(s) | 1424 |
| GsaI | CCCAGC | 3 cut(s) | 27, 1236, 1383 |
| HaeIII | GGCC | 6 cut(s) | 51, 289, 335, 489, 750, 1363 |
| HapII | CCGG | 1 cut(s) | 5 |
| Hin1II | CATG | 8 cut(s) | 322, 818, 1015, 1272, 1406, 1541, 1676, 1706 |
| HincII | GTYRAC | 1 cut(s) | 1113 |
| HindII | GTYRAC | 1 cut(s) | 1113 |
| HinfI | GANTC | 6 cut(s) | 110, 824, 866, 1172, 1259, 1634 |
| HpaII | CCGG | 1 cut(s) | 5 |
| HphI | GGTGA | 3 cut(s) | 74, 538, 1249 |
| Hpy166II | GTNNAC | 1 cut(s) | 1113 |
| Hpy188I | TCNGA | 7 cut(s) | 115, 262, 350, 871, 1441, 1624, 1639 |
| Hpy8I | GTNNAC | 1 cut(s) | 1113 |
| HpyAV | CCTTC | 4 cut(s) | 526, 740, 1021, 1593 |
| HpyCH4III | ACNGT | 1 cut(s) | 904 |
| HpyCH4IV | ACGT | 2 cut(s) | 585, 1502 |
| HpyCH4V | TGCA | 4 cut(s) | 844, 964, 1426, 1708 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1229, 1694 |
| HpySE526I | ACGT | 2 cut(s) | 585, 1502 |
| Hsp92II | CATG | 8 cut(s) | 322, 818, 1015, 1272, 1406, 1541, 1676, 1706 |
| Kzo9I | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| Lsp1109I | GCAGC | 1 cut(s) | 1410 |
| LweI | GCATC | 2 cut(s) | 643, 951 |
| MabI | ACCWGGT | 1 cut(s) | 855 |
| MaeI | CTAG | 4 cut(s) | 404, 1167, 1176, 1323 |
| MaeII | ACGT | 2 cut(s) | 585, 1502 |
| MaeIII | GTNAC | 1 cut(s) | 1256 |
| MalI | GATC | 8 cut(s) | 195, 352, 402, 545, 1150, 1195, 1542, 1605 |
| MboI | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| MboII | GAAGA | 7 cut(s) | 125, 347, 350, 455, 1015, 1145, 1381 |
| MflI | RGATCY | 4 cut(s) | 350, 400, 1148, 1193 |
| MhlI | GDGCHC | 5 cut(s) | 76, 278, 376, 462, 1439 |
| MlsI | TGGCCA | 1 cut(s) | 489 |
| MluNI | TGGCCA | 1 cut(s) | 489 |
| MlyI | GAGTC | 3 cut(s) | 875, 1253, 1643 |
| MmeI | TCCRAC | 2 cut(s) | 72, 1523 |
| Mox20I | TGGCCA | 1 cut(s) | 489 |
| MroXI | GAANNNNTTC | 1 cut(s) | 594 |
| MscI | TGGCCA | 1 cut(s) | 489 |
| MslI | CAYNNNNRTG | 3 cut(s) | 508, 551, 1271 |
| Msp20I | TGGCCA | 1 cut(s) | 489 |
| MspCI | CTTAAG | 1 cut(s) | 377 |
| MspI | CCGG | 1 cut(s) | 5 |
| MspR9I | CCNGG | 3 cut(s) | 83, 519, 857 |
| MvaI | CCWGG | 3 cut(s) | 83, 519, 857 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1229, 1694 |
| NdeI | CATATG | 1 cut(s) | 552 |
| NdeII | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| NlaIII | CATG | 8 cut(s) | 322, 818, 1015, 1272, 1406, 1541, 1676, 1706 |
| NlaIV | GGNNCC | 4 cut(s) | 37, 384, 861, 951 |
| NmuCI | GTSAC | 1 cut(s) | 1256 |
| NspI | RCATGY | 1 cut(s) | 1706 |
| NspV | TTCGAA | 1 cut(s) | 568 |
| OliI | CACNNNNGTG | 1 cut(s) | 1271 |
| PagI | TCATGA | 2 cut(s) | 1402, 1537 |
| PceI | AGGCCT | 1 cut(s) | 1363 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 119, 292 |
| PdmI | GAANNNNTTC | 1 cut(s) | 594 |
| PfeI | GAWTC | 3 cut(s) | 110, 824, 1172 |
| PkrI | GCNGC | 1 cut(s) | 1425 |
| PleI | GAGTC | 3 cut(s) | 874, 1253, 1642 |
| PpsI | GAGTC | 3 cut(s) | 874, 1253, 1642 |
| PpuMI | RGGWCCY | 1 cut(s) | 383 |
| PshBI | ATTAAT | 3 cut(s) | 573, 780, 1569 |
| Psp124BI | GAGCTC | 2 cut(s) | 76, 278 |
| Psp1406I | AACGTT | 2 cut(s) | 585, 1502 |
| Psp5II | RGGWCCY | 1 cut(s) | 383 |
| Psp6I | CCWGG | 3 cut(s) | 81, 517, 855 |
| PspFI | CCCAGC | 3 cut(s) | 23, 1232, 1379 |
| PspGI | CCWGG | 3 cut(s) | 81, 517, 855 |
| PspN4I | GGNNCC | 4 cut(s) | 37, 384, 861, 951 |
| PspPI | GGNCC | 3 cut(s) | 50, 383, 470 |
| PspPPI | RGGWCCY | 1 cut(s) | 383 |
| PsuI | RGATCY | 4 cut(s) | 350, 400, 1148, 1193 |
| RsaI | GTAC | 5 cut(s) | 79, 754, 906, 928, 1069 |
| RsaNI | GTAC | 5 cut(s) | 78, 753, 905, 927, 1068 |
| RseI | CAYNNNNRTG | 3 cut(s) | 508, 551, 1271 |
| SacI | GAGCTC | 2 cut(s) | 76, 278 |
| SatI | GCNGC | 1 cut(s) | 1424 |
| Sau3AI | GATC | 8 cut(s) | 193, 350, 400, 543, 1148, 1193, 1540, 1603 |
| Sau96I | GGNCC | 3 cut(s) | 50, 383, 470 |
| SchI | GAGTC | 3 cut(s) | 875, 1253, 1643 |
| ScrFI | CCNGG | 3 cut(s) | 83, 519, 857 |
| SduI | GDGCHC | 5 cut(s) | 76, 278, 376, 462, 1439 |
| SexAI | ACCWGGT | 1 cut(s) | 855 |
| SfaNI | GCATC | 2 cut(s) | 643, 951 |
| SfuI | TTCGAA | 1 cut(s) | 568 |
| SinI | GGWCC | 2 cut(s) | 383, 470 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 508, 551, 1271 |
| SmlI | CTYRAG | 2 cut(s) | 377, 1289 |
| SmoI | CTYRAG | 2 cut(s) | 377, 1289 |
| SpeI | ACTAGT | 1 cut(s) | 1166 |
| SseBI | AGGCCT | 1 cut(s) | 1363 |
| SsiI | CCGC | 2 cut(s) | 102, 188 |
| SspI | AATATT | 1 cut(s) | 593 |
| SspMI | CTAG | 4 cut(s) | 404, 1167, 1176, 1323 |
| SstI | GAGCTC | 2 cut(s) | 76, 278 |
| StuI | AGGCCT | 1 cut(s) | 1363 |
| StyD4I | CCNGG | 3 cut(s) | 81, 517, 855 |
| StyI | CCWWGG | 4 cut(s) | 290, 473, 989, 1364 |
| TaaI | ACNGT | 1 cut(s) | 904 |
| TaiI | ACGT | 2 cut(s) | 588, 1505 |
| TaqI | TCGA | 2 cut(s) | 568, 691 |
| TatI | WGTACW | 1 cut(s) | 904 |
| TfiI | GAWTC | 3 cut(s) | 110, 824, 1172 |
| TscAI | CASTG | 1 cut(s) | 469 |
| TseFI | GTSAC | 1 cut(s) | 1256 |
| TseI | GCWGC | 1 cut(s) | 1423 |
| Tsp45I | GTSAC | 1 cut(s) | 1256 |
| TspDTI | ATGAA | 4 cut(s) | 409, 724, 932, 1028 |
| TspGWI | ACGGA | 2 cut(s) | 656, 1297 |
| TspRI | CASTG | 1 cut(s) | 469 |
| Vha464I | CTTAAG | 1 cut(s) | 377 |
| VpaK11BI | GGWCC | 2 cut(s) | 383, 470 |
| VspI | ATTAAT | 3 cut(s) | 573, 780, 1569 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 437 |
| XapI | RAATTY | 4 cut(s) | 8, 693, 919, 994 |
| XbaI | TCTAGA | 1 cut(s) | 403 |
| XceI | RCATGY | 1 cut(s) | 1706 |
| XmnI | GAANNNNTTC | 1 cut(s) | 594 |
| XspI | CTAG | 4 cut(s) | 404, 1167, 1176, 1323 |