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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 246, 249 |
| AclWI | GGATC | 2 cut(s) | 266, 279 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 119, 192, 298 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 89 |
| AhlI | ACTAGT | 1 cut(s) | 88 |
| AjnI | CCWGG | 2 cut(s) | 37, 191 |
| AluBI | AGCT | 2 cut(s) | 10, 262 |
| AluI | AGCT | 2 cut(s) | 10, 262 |
| AlwI | GGATC | 2 cut(s) | 266, 279 |
| AoxI | GGCC | 1 cut(s) | 155 |
| AspS9I | GGNCC | 3 cut(s) | 41, 145, 156 |
| AsuHPI | GGTGA | 1 cut(s) | 223 |
| AvaII | GGWCC | 2 cut(s) | 41, 145 |
| BamHI | GGATCC | 1 cut(s) | 271 |
| BauI | CACGAG | 1 cut(s) | 115 |
| BbsI | GAAGAC | 1 cut(s) | 276 |
| BceAI | ACGGC | 1 cut(s) | 236 |
| BciT130I | CCWGG | 2 cut(s) | 39, 193 |
| BciVI | GTATCC | 1 cut(s) | 116 |
| BcuI | ACTAGT | 1 cut(s) | 88 |
| BfaI | CTAG | 2 cut(s) | 89, 171 |
| BfuI | GTATCC | 1 cut(s) | 116 |
| BisI | GCNGC | 1 cut(s) | 249 |
| BlsI | GCNGC | 1 cut(s) | 250 |
| Bme1390I | CCNGG | 2 cut(s) | 39, 193 |
| Bme18I | GGWCC | 2 cut(s) | 41, 145 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 89 |
| BmgT120I | GGNCC | 3 cut(s) | 41, 145, 156 |
| BmiI | GGNNCC | 2 cut(s) | 146, 273 |
| BmrFI | CCNGG | 2 cut(s) | 39, 193 |
| BpiI | GAAGAC | 1 cut(s) | 276 |
| BsaJI | CCNNGG | 1 cut(s) | 191 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 119, 192, 298 |
| BseBI | CCWGG | 2 cut(s) | 39, 193 |
| BseDI | CCNNGG | 1 cut(s) | 191 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 119, 192, 298 |
| BseYI | CCCAGC | 1 cut(s) | 186 |
| Bsh1285I | CGRYCG | 1 cut(s) | 303 |
| BshFI | GGCC | 1 cut(s) | 157 |
| BsiEI | CGRYCG | 1 cut(s) | 303 |
| BslFI | GGGAC | 1 cut(s) | 96 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 119, 192, 298 |
| BsmFI | GGGAC | 1 cut(s) | 96 |
| BsnI | GGCC | 1 cut(s) | 157 |
| Bsp143I | GATC | 1 cut(s) | 271 |
| BspACI | CCGC | 2 cut(s) | 246, 249 |
| BspANI | GGCC | 1 cut(s) | 157 |
| BspLI | GGNNCC | 2 cut(s) | 146, 273 |
| BspPI | GGATC | 2 cut(s) | 266, 279 |
| BssECI | CCNNGG | 1 cut(s) | 191 |
| BssMI | GATC | 1 cut(s) | 271 |
| BssSI | CACGAG | 1 cut(s) | 115 |
| Bst2BI | CACGAG | 1 cut(s) | 115 |
| Bst2UI | CCWGG | 2 cut(s) | 39, 193 |
| Bst4CI | ACNGT | 2 cut(s) | 110, 282 |
| BstEII | GGTNACC | 1 cut(s) | 202 |
| BstKTI | GATC | 1 cut(s) | 274 |
| BstMBI | GATC | 1 cut(s) | 271 |
| BstMCI | CGRYCG | 1 cut(s) | 303 |
| BstNI | CCWGG | 2 cut(s) | 39, 193 |
| BstPI | GGTNACC | 1 cut(s) | 202 |
| BstSCI | CCNGG | 2 cut(s) | 37, 191 |
| BstV2I | GAAGAC | 1 cut(s) | 276 |
| BstX2I | RGATCY | 1 cut(s) | 271 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 199 |
| BstYI | RGATCY | 1 cut(s) | 271 |
| BsuI | GTATCC | 1 cut(s) | 116 |
| BsuRI | GGCC | 1 cut(s) | 157 |
| Cfr13I | GGNCC | 3 cut(s) | 41, 145, 156 |
| CviAII | CATG | 3 cut(s) | 153, 268, 306 |
| CviJI | RGCY | 5 cut(s) | 10, 157, 190, 213, 262 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 157, 190, 213, 262 |
| DpnI | GATC | 1 cut(s) | 273 |
| DpnII | GATC | 1 cut(s) | 271 |
| DriI | GACNNNNNGTC | 1 cut(s) | 89 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 89 |
| EciI | GGCGGA | 1 cut(s) | 235 |
| Eco47I | GGWCC | 2 cut(s) | 41, 145 |
| Eco91I | GGTNACC | 1 cut(s) | 202 |
| EcoO65I | GGTNACC | 1 cut(s) | 202 |
| EcoRII | CCWGG | 2 cut(s) | 37, 191 |
| FaeI | CATG | 3 cut(s) | 156, 271, 309 |
| FaiI | YATR | 5 cut(s) | 21, 128, 154, 269, 307 |
| FaqI | GGGAC | 1 cut(s) | 96 |
| FatI | CATG | 3 cut(s) | 152, 267, 305 |
| Fnu4HI | GCNGC | 1 cut(s) | 249 |
| Fsp4HI | GCNGC | 1 cut(s) | 249 |
| FspBI | CTAG | 2 cut(s) | 89, 171 |
| GluI | GCNGC | 1 cut(s) | 249 |
| GsaI | CCCAGC | 1 cut(s) | 190 |
| HaeIII | GGCC | 1 cut(s) | 157 |
| Hin1II | CATG | 3 cut(s) | 156, 271, 309 |
| HinfI | GANTC | 1 cut(s) | 242 |
| HphI | GGTGA | 1 cut(s) | 223 |
| Hpy188I | TCNGA | 1 cut(s) | 257 |
| HpyAV | CCTTC | 2 cut(s) | 238, 298 |
| HpyCH4III | ACNGT | 2 cut(s) | 110, 282 |
| Hsp92II | CATG | 3 cut(s) | 156, 271, 309 |
| Kzo9I | GATC | 1 cut(s) | 271 |
| LpnPI | CCDG | 5 cut(s) | 24, 51, 178, 200, 205 |
| MaeI | CTAG | 2 cut(s) | 89, 171 |
| MaeIII | GTNAC | 1 cut(s) | 202 |
| MalI | GATC | 1 cut(s) | 273 |
| MboI | GATC | 1 cut(s) | 271 |
| MboII | GAAGA | 1 cut(s) | 276 |
| MflI | RGATCY | 1 cut(s) | 271 |
| MnlI | CCTC | 3 cut(s) | 54, 111, 285 |
| MseI | TTAA | 1 cut(s) | 75 |
| MspR9I | CCNGG | 2 cut(s) | 39, 193 |
| MvaI | CCWGG | 2 cut(s) | 39, 193 |
| NdeII | GATC | 1 cut(s) | 271 |
| NlaIII | CATG | 3 cut(s) | 156, 271, 309 |
| NlaIV | GGNNCC | 2 cut(s) | 146, 273 |
| PfeI | GAWTC | 1 cut(s) | 242 |
| PkrI | GCNGC | 1 cut(s) | 250 |
| Psp6I | CCWGG | 2 cut(s) | 37, 191 |
| PspEI | GGTNACC | 1 cut(s) | 202 |
| PspFI | CCCAGC | 1 cut(s) | 186 |
| PspGI | CCWGG | 2 cut(s) | 37, 191 |
| PspN4I | GGNNCC | 2 cut(s) | 146, 273 |
| PspPI | GGNCC | 3 cut(s) | 41, 145, 156 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 18, 50 |
| PsuI | RGATCY | 1 cut(s) | 271 |
| SaqAI | TTAA | 1 cut(s) | 75 |
| SatI | GCNGC | 1 cut(s) | 249 |
| Sau3AI | GATC | 1 cut(s) | 271 |
| Sau96I | GGNCC | 3 cut(s) | 41, 145, 156 |
| ScrFI | CCNGG | 2 cut(s) | 39, 193 |
| SetI | ASST | 5 cut(s) | 12, 40, 46, 197, 264 |
| SinI | GGWCC | 2 cut(s) | 41, 145 |
| SpeI | ACTAGT | 1 cut(s) | 88 |
| SsiI | CCGC | 2 cut(s) | 246, 249 |
| SspMI | CTAG | 2 cut(s) | 89, 171 |
| StyD4I | CCNGG | 2 cut(s) | 37, 191 |
| TaaI | ACNGT | 2 cut(s) | 110, 282 |
| TaqII | GACCGA | 1 cut(s) | 289 |
| TauI | GCSGC | 1 cut(s) | 251 |
| TfiI | GAWTC | 1 cut(s) | 242 |
| Tru1I | TTAA | 1 cut(s) | 75 |
| Tru9I | TTAA | 1 cut(s) | 75 |
| TspDTI | ATGAA | 1 cut(s) | 39 |
| TspGWI | ACGGA | 1 cut(s) | 96 |
| VpaK11BI | GGWCC | 2 cut(s) | 41, 145 |
| XspI | CTAG | 2 cut(s) | 89, 171 |