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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 267 |
| AciI | CCGC | 1 cut(s) | 225 |
| AclWI | GGATC | 1 cut(s) | 617 |
| AcsI | RAATTY | 1 cut(s) | 655 |
| AcuI | CTGAAG | 1 cut(s) | 294 |
| AfaI | GTAC | 3 cut(s) | 463, 673, 835 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 149, 782 |
| AflII | CTTAAG | 1 cut(s) | 497 |
| AgsI | TTSAA | 1 cut(s) | 616 |
| AhlI | ACTAGT | 1 cut(s) | 830 |
| AjnI | CCWGG | 2 cut(s) | 161, 235 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 640, 672 |
| AluBI | AGCT | 4 cut(s) | 209, 629, 742, 860 |
| AluI | AGCT | 4 cut(s) | 209, 629, 742, 860 |
| Alw26I | GTCTC | 1 cut(s) | 410 |
| AlwI | GGATC | 1 cut(s) | 617 |
| AoxI | GGCC | 1 cut(s) | 799 |
| ApoI | RAATTY | 1 cut(s) | 655 |
| AspS9I | GGNCC | 2 cut(s) | 158, 800 |
| AsuC2I | CCSGG | 1 cut(s) | 803 |
| AsuHPI | GGTGA | 2 cut(s) | 290, 413 |
| AvaII | GGWCC | 1 cut(s) | 158 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 663, 696 |
| BbsI | GAAGAC | 2 cut(s) | 551, 570 |
| BbvCI | CCTCAGC | 1 cut(s) | 591 |
| BccI | CCATC | 4 cut(s) | 114, 152, 260, 766 |
| BciT130I | CCWGG | 2 cut(s) | 163, 237 |
| BclI | TGATCA | 3 cut(s) | 316, 468, 663 |
| BcnI | CCSGG | 1 cut(s) | 803 |
| BcoDI | GTCTC | 1 cut(s) | 410 |
| BcuI | ACTAGT | 1 cut(s) | 830 |
| BfaI | CTAG | 5 cut(s) | 213, 630, 831, 861, 885 |
| BfmI | CTRYAG | 1 cut(s) | 855 |
| BfrI | CTTAAG | 1 cut(s) | 497 |
| BisI | GCNGC | 1 cut(s) | 225 |
| BlsI | GCNGC | 1 cut(s) | 226 |
| BmcAI | AGTACT | 2 cut(s) | 463, 835 |
| Bme1390I | CCNGG | 3 cut(s) | 163, 237, 803 |
| Bme18I | GGWCC | 1 cut(s) | 158 |
| BmgT120I | GGNCC | 2 cut(s) | 158, 800 |
| BmiI | GGNNCC | 2 cut(s) | 160, 241 |
| BmrFI | CCNGG | 3 cut(s) | 163, 237, 803 |
| BmsI | GCATC | 1 cut(s) | 127 |
| BpiI | GAAGAC | 2 cut(s) | 551, 570 |
| BpmI | CTGGAG | 2 cut(s) | 84, 117 |
| Bpu10I | CCTNAGC | 1 cut(s) | 591 |
| BpuEI | CTTGAG | 2 cut(s) | 758, 762 |
| BpuMI | CCSGG | 1 cut(s) | 803 |
| BsaJI | CCNNGG | 1 cut(s) | 161 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 82, 112 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 149, 782 |
| Bse1I | ACTGG | 1 cut(s) | 101 |
| BseBI | CCWGG | 2 cut(s) | 163, 237 |
| BseDI | CCNNGG | 1 cut(s) | 161 |
| BseGI | GGATG | 3 cut(s) | 171, 252, 319 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 149, 782 |
| BseMII | CTCAG | 2 cut(s) | 435, 605 |
| BseNI | ACTGG | 1 cut(s) | 101 |
| BshFI | GGCC | 1 cut(s) | 801 |
| BsiSI | CCGG | 1 cut(s) | 803 |
| BslFI | GGGAC | 1 cut(s) | 569 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 149, 782 |
| BsmAI | GTCTC | 1 cut(s) | 410 |
| BsmFI | GGGAC | 1 cut(s) | 569 |
| BsmI | GAATGC | 1 cut(s) | 289 |
| BsnI | GGCC | 1 cut(s) | 801 |
| Bsp143I | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| BspACI | CCGC | 1 cut(s) | 225 |
| BspANI | GGCC | 1 cut(s) | 801 |
| BspCNI | CTCAG | 2 cut(s) | 436, 604 |
| BspLI | GGNNCC | 2 cut(s) | 160, 241 |
| BspPI | GGATC | 1 cut(s) | 617 |
| BspTI | CTTAAG | 1 cut(s) | 497 |
| BsrI | ACTGG | 1 cut(s) | 101 |
| BssECI | CCNNGG | 1 cut(s) | 161 |
| BssMI | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| Bst2UI | CCWGG | 2 cut(s) | 163, 237 |
| Bst4CI | ACNGT | 3 cut(s) | 200, 466, 722 |
| BstAFI | CTTAAG | 1 cut(s) | 497 |
| BstC8I | GCNNGC | 1 cut(s) | 329 |
| BstDEI | CTNAG | 3 cut(s) | 444, 558, 591 |
| BstF5I | GGATG | 3 cut(s) | 171, 252, 319 |
| BstKTI | GATC | 5 cut(s) | 67, 319, 471, 625, 666 |
| BstMAI | GTCTC | 1 cut(s) | 410 |
| BstMBI | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| BstNI | CCWGG | 2 cut(s) | 163, 237 |
| BstSCI | CCNGG | 3 cut(s) | 161, 235, 801 |
| BstSFI | CTRYAG | 1 cut(s) | 855 |
| BstV2I | GAAGAC | 2 cut(s) | 551, 570 |
| BsuRI | GGCC | 1 cut(s) | 801 |
| BtsCI | GGATG | 3 cut(s) | 171, 252, 319 |
| BtsI | GCAGTG | 1 cut(s) | 802 |
| BtsIMutI | CAGTG | 2 cut(s) | 205, 802 |
| Cac8I | GCNNGC | 1 cut(s) | 329 |
| Cfr13I | GGNCC | 2 cut(s) | 158, 800 |
| CsiI | ACCWGGT | 1 cut(s) | 235 |
| Csp6I | GTAC | 3 cut(s) | 462, 672, 834 |
| CviAII | CATG | 5 cut(s) | 85, 155, 332, 620, 677 |
| CviJI | RGCY | 9 cut(s) | 209, 392, 494, 595, 629, 742, 801, 860, 902 |
| CviKI_1 | RGCY | 9 cut(s) | 209, 392, 494, 595, 629, 742, 801, 860, 902 |
| CviQI | GTAC | 3 cut(s) | 462, 672, 834 |
| DdeI | CTNAG | 3 cut(s) | 444, 558, 591 |
| DpnI | GATC | 5 cut(s) | 66, 318, 470, 624, 665 |
| DpnII | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| DraI | TTTAAA | 1 cut(s) | 654 |
| Eco47I | GGWCC | 1 cut(s) | 158 |
| Eco57I | CTGAAG | 1 cut(s) | 294 |
| EcoRII | CCWGG | 2 cut(s) | 161, 235 |
| FaeI | CATG | 5 cut(s) | 88, 158, 335, 623, 680 |
| FaqI | GGGAC | 1 cut(s) | 569 |
| FatI | CATG | 5 cut(s) | 84, 154, 331, 619, 676 |
| FbaI | TGATCA | 3 cut(s) | 316, 468, 663 |
| Fnu4HI | GCNGC | 1 cut(s) | 225 |
| FokI | GGATG | 3 cut(s) | 178, 239, 326 |
| Fsp4HI | GCNGC | 1 cut(s) | 225 |
| FspBI | CTAG | 5 cut(s) | 213, 630, 831, 861, 885 |
| GluI | GCNGC | 1 cut(s) | 225 |
| GsuI | CTGGAG | 2 cut(s) | 84, 117 |
| HaeIII | GGCC | 1 cut(s) | 801 |
| HapII | CCGG | 1 cut(s) | 803 |
| Hin1II | CATG | 5 cut(s) | 88, 158, 335, 623, 680 |
| HinfI | GANTC | 4 cut(s) | 125, 448, 537, 700 |
| HpaII | CCGG | 1 cut(s) | 803 |
| HphI | GGTGA | 2 cut(s) | 290, 413 |
| Hpy166II | GTNNAC | 1 cut(s) | 452 |
| Hpy188I | TCNGA | 5 cut(s) | 346, 568, 663, 814, 841 |
| Hpy188III | TCNNGA | 3 cut(s) | 134, 541, 737 |
| Hpy8I | GTNNAC | 1 cut(s) | 452 |
| HpyAV | CCTTC | 2 cut(s) | 269, 696 |
| HpyCH4III | ACNGT | 3 cut(s) | 200, 466, 722 |
| HpyCH4V | TGCA | 4 cut(s) | 140, 259, 292, 795 |
| HpyF3I | CTNAG | 3 cut(s) | 444, 558, 591 |
| Hsp92II | CATG | 5 cut(s) | 88, 158, 335, 623, 680 |
| Ksp22I | TGATCA | 3 cut(s) | 316, 468, 663 |
| Kzo9I | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| LmnI | GCTCC | 1 cut(s) | 214 |
| LweI | GCATC | 1 cut(s) | 127 |
| MabI | ACCWGGT | 1 cut(s) | 235 |
| MaeI | CTAG | 5 cut(s) | 213, 630, 831, 861, 885 |
| MalI | GATC | 5 cut(s) | 66, 318, 470, 624, 665 |
| MboI | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| MboII | GAAGA | 6 cut(s) | 120, 308, 333, 556, 570, 753 |
| MluCI | AATT | 7 cut(s) | 354, 386, 472, 655, 706, 732, 907 |
| MlyI | GAGTC | 1 cut(s) | 457 |
| MnlI | CCTC | 7 cut(s) | 600, 648, 700, 735, 786, 886, 892 |
| MseI | TTAA | 6 cut(s) | 170, 378, 498, 573, 653, 910 |
| MspCI | CTTAAG | 1 cut(s) | 497 |
| MspI | CCGG | 1 cut(s) | 803 |
| MspR9I | CCNGG | 3 cut(s) | 163, 237, 803 |
| Mva1269I | GAATGC | 1 cut(s) | 289 |
| MvaI | CCWGG | 2 cut(s) | 163, 237 |
| NciI | CCSGG | 1 cut(s) | 803 |
| NdeII | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| NlaIII | CATG | 5 cut(s) | 88, 158, 335, 623, 680 |
| NlaIV | GGNNCC | 2 cut(s) | 160, 241 |
| PctI | GAATGC | 1 cut(s) | 289 |
| PfeI | GAWTC | 3 cut(s) | 125, 537, 700 |
| PflFI | GACNNNGTC | 1 cut(s) | 720 |
| PkrI | GCNGC | 1 cut(s) | 226 |
| PleI | GAGTC | 1 cut(s) | 456 |
| PpsI | GAGTC | 1 cut(s) | 456 |
| PsiI | TTATAA | 1 cut(s) | 267 |
| Psp6I | CCWGG | 2 cut(s) | 161, 235 |
| PspGI | CCWGG | 2 cut(s) | 161, 235 |
| PspN4I | GGNNCC | 2 cut(s) | 160, 241 |
| PspPI | GGNCC | 2 cut(s) | 158, 800 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 291, 323 |
| PsyI | GACNNNGTC | 1 cut(s) | 720 |
| RsaI | GTAC | 3 cut(s) | 463, 673, 835 |
| RsaNI | GTAC | 3 cut(s) | 462, 672, 834 |
| SaqAI | TTAA | 6 cut(s) | 170, 378, 498, 573, 653, 910 |
| SatI | GCNGC | 1 cut(s) | 225 |
| Sau3AI | GATC | 5 cut(s) | 64, 316, 468, 622, 663 |
| Sau96I | GGNCC | 2 cut(s) | 158, 800 |
| ScaI | AGTACT | 2 cut(s) | 463, 835 |
| SchI | GAGTC | 1 cut(s) | 457 |
| ScrFI | CCNGG | 3 cut(s) | 163, 237, 803 |
| SexAI | ACCWGGT | 1 cut(s) | 235 |
| SfaNI | GCATC | 1 cut(s) | 127 |
| SfcI | CTRYAG | 1 cut(s) | 855 |
| SinI | GGWCC | 1 cut(s) | 158 |
| SmlI | CTYRAG | 3 cut(s) | 497, 737, 777 |
| SmoI | CTYRAG | 3 cut(s) | 497, 737, 777 |
| SpeI | ACTAGT | 1 cut(s) | 830 |
| Sse9I | AATT | 7 cut(s) | 354, 386, 472, 655, 706, 732, 907 |
| SsiI | CCGC | 1 cut(s) | 225 |
| SspI | AATATT | 1 cut(s) | 772 |
| SspMI | CTAG | 5 cut(s) | 213, 630, 831, 861, 885 |
| StyD4I | CCNGG | 3 cut(s) | 161, 235, 801 |
| TaaI | ACNGT | 3 cut(s) | 200, 466, 722 |
| TasI | AATT | 7 cut(s) | 354, 386, 472, 655, 706, 732, 907 |
| TatI | WGTACW | 2 cut(s) | 461, 833 |
| TauI | GCSGC | 1 cut(s) | 227 |
| TfiI | GAWTC | 3 cut(s) | 125, 537, 700 |
| Tru1I | TTAA | 6 cut(s) | 170, 378, 498, 573, 653, 910 |
| Tru9I | TTAA | 6 cut(s) | 170, 378, 498, 573, 653, 910 |
| TscAI | CASTG | 2 cut(s) | 205, 802 |
| TspDTI | ATGAA | 4 cut(s) | 209, 699, 782, 839 |
| TspGWI | ACGGA | 1 cut(s) | 571 |
| TspRI | CASTG | 2 cut(s) | 205, 802 |
| Tth111I | GACNNNGTC | 1 cut(s) | 720 |
| Vha464I | CTTAAG | 1 cut(s) | 497 |
| VpaK11BI | GGWCC | 1 cut(s) | 158 |
| XapI | RAATTY | 1 cut(s) | 655 |
| XspI | CTAG | 5 cut(s) | 213, 630, 831, 861, 885 |
| ZrmI | AGTACT | 2 cut(s) | 463, 835 |