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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 167 |
| Acc36I | ACCTGC | 1 cut(s) | 228 |
| AfaI | GTAC | 1 cut(s) | 190 |
| AluBI | AGCT | 1 cut(s) | 240 |
| AluI | AGCT | 1 cut(s) | 240 |
| BfmI | CTRYAG | 1 cut(s) | 221 |
| BfuAI | ACCTGC | 1 cut(s) | 228 |
| Bsp1407I | TGTACA | 1 cut(s) | 188 |
| BspMAI | CTGCAG | 1 cut(s) | 225 |
| BspMI | ACCTGC | 1 cut(s) | 228 |
| BsrGI | TGTACA | 1 cut(s) | 188 |
| BstAUI | TGTACA | 1 cut(s) | 188 |
| BstNSI | RCATGY | 1 cut(s) | 116 |
| BstSFI | CTRYAG | 1 cut(s) | 221 |
| BveI | ACCTGC | 1 cut(s) | 228 |
| Csp6I | GTAC | 1 cut(s) | 189 |
| CviAII | CATG | 1 cut(s) | 113 |
| CviJI | RGCY | 2 cut(s) | 194, 240 |
| CviKI_1 | RGCY | 2 cut(s) | 194, 240 |
| CviQI | GTAC | 1 cut(s) | 189 |
| FaeI | CATG | 1 cut(s) | 116 |
| FaiI | YATR | 7 cut(s) | 106, 114, 130, 132, 140, 167, 187 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 70, 102 |
| FatI | CATG | 1 cut(s) | 112 |
| Hin1II | CATG | 1 cut(s) | 116 |
| Hpy188III | TCNNGA | 2 cut(s) | 12, 63 |
| HpyCH4V | TGCA | 2 cut(s) | 32, 223 |
| Hsp92II | CATG | 1 cut(s) | 116 |
| LpnPI | CCDG | 4 cut(s) | 48, 131, 195, 233 |
| MboII | GAAGA | 1 cut(s) | 225 |
| MnlI | CCTC | 2 cut(s) | 65, 205 |
| NlaIII | CATG | 1 cut(s) | 116 |
| NspI | RCATGY | 1 cut(s) | 116 |
| PsiI | TTATAA | 1 cut(s) | 167 |
| PstI | CTGCAG | 1 cut(s) | 225 |
| RsaI | GTAC | 1 cut(s) | 190 |
| RsaNI | GTAC | 1 cut(s) | 189 |
| SetI | ASST | 2 cut(s) | 222, 242 |
| SfcI | CTRYAG | 1 cut(s) | 221 |
| SgeI | CNNG | 9 cut(s) | 24, 36, 75, 94, 125, 130, 185, 222, 232 |
| SspI | AATATT | 1 cut(s) | 36 |
| TatI | WGTACW | 1 cut(s) | 188 |
| TspDTI | ATGAA | 1 cut(s) | 48 |
| XceI | RCATGY | 1 cut(s) | 116 |