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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 128 |
| AfaI | GTAC | 1 cut(s) | 184 |
| AflII | CTTAAG | 1 cut(s) | 8 |
| AgsI | TTSAA | 1 cut(s) | 127 |
| AjnI | CCWGG | 1 cut(s) | 140 |
| AlwI | GGATC | 1 cut(s) | 128 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 174, 207 |
| BbsI | GAAGAC | 2 cut(s) | 62, 81 |
| BbvCI | CCTCAGC | 1 cut(s) | 102 |
| BciT130I | CCWGG | 1 cut(s) | 142 |
| BclI | TGATCA | 1 cut(s) | 174 |
| BfaI | CTAG | 1 cut(s) | 205 |
| BfrI | CTTAAG | 1 cut(s) | 8 |
| Bme1390I | CCNGG | 1 cut(s) | 142 |
| BmrFI | CCNGG | 1 cut(s) | 142 |
| BpiI | GAAGAC | 2 cut(s) | 62, 81 |
| Bpu10I | CCTNAGC | 1 cut(s) | 102 |
| BseBI | CCWGG | 1 cut(s) | 142 |
| BseMII | CTCAG | 1 cut(s) | 116 |
| BslFI | GGGAC | 1 cut(s) | 80 |
| BsmFI | GGGAC | 1 cut(s) | 80 |
| Bsp143I | GATC | 2 cut(s) | 133, 174 |
| BspCNI | CTCAG | 1 cut(s) | 115 |
| BspPI | GGATC | 1 cut(s) | 128 |
| BspTI | CTTAAG | 1 cut(s) | 8 |
| BssMI | GATC | 2 cut(s) | 133, 174 |
| Bst2UI | CCWGG | 1 cut(s) | 142 |
| BstAFI | CTTAAG | 1 cut(s) | 8 |
| BstDEI | CTNAG | 2 cut(s) | 69, 102 |
| BstKTI | GATC | 2 cut(s) | 136, 177 |
| BstMBI | GATC | 2 cut(s) | 133, 174 |
| BstNI | CCWGG | 1 cut(s) | 142 |
| BstSCI | CCNGG | 1 cut(s) | 140 |
| BstV2I | GAAGAC | 2 cut(s) | 62, 81 |
| Csp6I | GTAC | 1 cut(s) | 183 |
| CviAII | CATG | 2 cut(s) | 131, 188 |
| CviJI | RGCY | 3 cut(s) | 5, 106, 140 |
| CviKI_1 | RGCY | 3 cut(s) | 5, 106, 140 |
| CviQI | GTAC | 1 cut(s) | 183 |
| DdeI | CTNAG | 2 cut(s) | 69, 102 |
| DpnI | GATC | 2 cut(s) | 135, 176 |
| DpnII | GATC | 2 cut(s) | 133, 174 |
| DraI | TTTAAA | 1 cut(s) | 165 |
| EcoRII | CCWGG | 1 cut(s) | 140 |
| FaeI | CATG | 2 cut(s) | 134, 191 |
| FaiI | YATR | 2 cut(s) | 132, 189 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 101, 133 |
| FaqI | GGGAC | 1 cut(s) | 80 |
| FatI | CATG | 2 cut(s) | 130, 187 |
| FbaI | TGATCA | 1 cut(s) | 174 |
| FspBI | CTAG | 1 cut(s) | 205 |
| Hin1II | CATG | 2 cut(s) | 134, 191 |
| HinfI | GANTC | 1 cut(s) | 48 |
| Hpy188I | TCNGA | 2 cut(s) | 79, 174 |
| Hpy188III | TCNNGA | 1 cut(s) | 52 |
| HpyAV | CCTTC | 1 cut(s) | 207 |
| HpyF3I | CTNAG | 2 cut(s) | 69, 102 |
| Hsp92II | CATG | 2 cut(s) | 134, 191 |
| Ksp22I | TGATCA | 1 cut(s) | 174 |
| Kzo9I | GATC | 2 cut(s) | 133, 174 |
| LpnPI | CCDG | 4 cut(s) | 48, 127, 150, 154 |
| MaeI | CTAG | 1 cut(s) | 205 |
| MalI | GATC | 2 cut(s) | 135, 176 |
| MboI | GATC | 2 cut(s) | 133, 174 |
| MboII | GAAGA | 2 cut(s) | 67, 81 |
| MnlI | CCTC | 2 cut(s) | 111, 159 |
| MseI | TTAA | 3 cut(s) | 9, 84, 164 |
| MspCI | CTTAAG | 1 cut(s) | 8 |
| MspR9I | CCNGG | 1 cut(s) | 142 |
| MvaI | CCWGG | 1 cut(s) | 142 |
| NdeII | GATC | 2 cut(s) | 133, 174 |
| NlaIII | CATG | 2 cut(s) | 134, 191 |
| PfeI | GAWTC | 1 cut(s) | 48 |
| Psp6I | CCWGG | 1 cut(s) | 140 |
| PspGI | CCWGG | 1 cut(s) | 140 |
| RsaI | GTAC | 1 cut(s) | 184 |
| RsaNI | GTAC | 1 cut(s) | 183 |
| SaqAI | TTAA | 3 cut(s) | 9, 84, 164 |
| Sau3AI | GATC | 2 cut(s) | 133, 174 |
| ScrFI | CCNGG | 1 cut(s) | 142 |
| SetI | ASST | 2 cut(s) | 103, 151 |
| SgeI | CNNG | 9 cut(s) | 47, 64, 121, 143, 149, 153, 154, 171, 200 |
| SmlI | CTYRAG | 1 cut(s) | 8 |
| SmoI | CTYRAG | 1 cut(s) | 8 |
| SspMI | CTAG | 1 cut(s) | 205 |
| StyD4I | CCNGG | 1 cut(s) | 140 |
| TfiI | GAWTC | 1 cut(s) | 48 |
| Tru1I | TTAA | 3 cut(s) | 9, 84, 164 |
| Tru9I | TTAA | 3 cut(s) | 9, 84, 164 |
| TspGWI | ACGGA | 1 cut(s) | 82 |
| Vha464I | CTTAAG | 1 cut(s) | 8 |
| XspI | CTAG | 1 cut(s) | 205 |