No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 244 |
| AccIII | TCCGGA | 1 cut(s) | 308 |
| AclWI | GGATC | 1 cut(s) | 319 |
| AcoI | YGGCCR | 2 cut(s) | 40, 634 |
| AcsI | RAATTY | 4 cut(s) | 377, 394, 545, 577 |
| AcuI | CTGAAG | 1 cut(s) | 474 |
| AdeI | CACNNNGTG | 1 cut(s) | 424 |
| AfaI | GTAC | 2 cut(s) | 189, 574 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 549 |
| AgsI | TTSAA | 4 cut(s) | 132, 266, 382, 512 |
| AjnI | CCWGG | 2 cut(s) | 114, 384 |
| AluBI | AGCT | 2 cut(s) | 504, 570 |
| AluI | AGCT | 2 cut(s) | 504, 570 |
| Alw26I | GTCTC | 2 cut(s) | 143, 355 |
| AlwI | GGATC | 1 cut(s) | 319 |
| Aor13HI | TCCGGA | 1 cut(s) | 308 |
| AoxI | GGCC | 3 cut(s) | 40, 81, 634 |
| ApoI | RAATTY | 4 cut(s) | 377, 394, 545, 577 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 569, 601 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 394 |
| AspS9I | GGNCC | 1 cut(s) | 47 |
| AsuHPI | GGTGA | 1 cut(s) | 176 |
| AvaII | GGWCC | 1 cut(s) | 47 |
| BaeGI | GKGCMC | 1 cut(s) | 115 |
| BalI | TGGCCA | 2 cut(s) | 42, 636 |
| BbsI | GAAGAC | 1 cut(s) | 480 |
| BccI | CCATC | 2 cut(s) | 373, 611 |
| BciT130I | CCWGG | 2 cut(s) | 116, 386 |
| BclI | TGATCA | 2 cut(s) | 13, 282 |
| BcoDI | GTCTC | 2 cut(s) | 143, 355 |
| BfaI | CTAG | 2 cut(s) | 354, 627 |
| BfmI | CTRYAG | 1 cut(s) | 237 |
| BfuAI | ACCTGC | 1 cut(s) | 244 |
| Bme1390I | CCNGG | 2 cut(s) | 116, 386 |
| Bme18I | GGWCC | 1 cut(s) | 47 |
| BmgT120I | GGNCC | 1 cut(s) | 47 |
| BmrFI | CCNGG | 2 cut(s) | 116, 386 |
| BmsI | GCATC | 2 cut(s) | 45, 205 |
| BpiI | GAAGAC | 1 cut(s) | 480 |
| BpuEI | CTTGAG | 1 cut(s) | 167 |
| BsaBI | GATNNNNATC | 1 cut(s) | 519 |
| BsaI | GGTCTC | 1 cut(s) | 355 |
| BsaJI | CCNNGG | 4 cut(s) | 43, 50, 114, 385 |
| BsaWI | WCCGGW | 1 cut(s) | 308 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 549 |
| Bse1I | ACTGG | 1 cut(s) | 484 |
| Bse3DI | GCAATG | 1 cut(s) | 637 |
| Bse8I | GATNNNNATC | 1 cut(s) | 519 |
| BseAI | TCCGGA | 1 cut(s) | 308 |
| BseBI | CCWGG | 2 cut(s) | 116, 386 |
| BseDI | CCNNGG | 4 cut(s) | 43, 50, 114, 385 |
| BseGI | GGATG | 2 cut(s) | 60, 520 |
| BseJI | GATNNNNATC | 1 cut(s) | 519 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 549 |
| BseMI | GCAATG | 1 cut(s) | 637 |
| BseMII | CTCAG | 4 cut(s) | 329, 473, 519, 555 |
| BseNI | ACTGG | 1 cut(s) | 484 |
| BseRI | GAGGAG | 1 cut(s) | 182 |
| BseSI | GKGCMC | 1 cut(s) | 115 |
| BsgI | GTGCAG | 1 cut(s) | 140 |
| BshFI | GGCC | 3 cut(s) | 42, 83, 636 |
| BsiSI | CCGG | 1 cut(s) | 309 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 549 |
| BsmAI | GTCTC | 2 cut(s) | 143, 355 |
| BsmI | GAATGC | 1 cut(s) | 178 |
| BsnI | GGCC | 3 cut(s) | 42, 83, 636 |
| Bso31I | GGTCTC | 1 cut(s) | 355 |
| Bsp1286I | GDGCHC | 1 cut(s) | 115 |
| Bsp13I | TCCGGA | 1 cut(s) | 308 |
| Bsp1407I | TGTACA | 1 cut(s) | 572 |
| Bsp143I | GATC | 3 cut(s) | 13, 282, 311 |
| Bsp19I | CCATGG | 1 cut(s) | 50 |
| BspANI | GGCC | 3 cut(s) | 42, 83, 636 |
| BspCNI | CTCAG | 4 cut(s) | 328, 472, 518, 556 |
| BspEI | TCCGGA | 1 cut(s) | 308 |
| BspMAI | CTGCAG | 1 cut(s) | 241 |
| BspMI | ACCTGC | 1 cut(s) | 244 |
| BspPI | GGATC | 1 cut(s) | 319 |
| BspTNI | GGTCTC | 1 cut(s) | 355 |
| BsrDI | GCAATG | 1 cut(s) | 637 |
| BsrGI | TGTACA | 1 cut(s) | 572 |
| BsrI | ACTGG | 1 cut(s) | 484 |
| BssECI | CCNNGG | 4 cut(s) | 43, 50, 114, 385 |
| BssMI | GATC | 3 cut(s) | 13, 282, 311 |
| BssT1I | CCWWGG | 1 cut(s) | 50 |
| Bst2UI | CCWGG | 2 cut(s) | 116, 386 |
| Bst4CI | ACNGT | 1 cut(s) | 110 |
| BstAUI | TGTACA | 1 cut(s) | 572 |
| BstC8I | GCNNGC | 2 cut(s) | 27, 302 |
| BstDEI | CTNAG | 4 cut(s) | 315, 459, 505, 564 |
| BstDSI | CCRYGG | 2 cut(s) | 43, 50 |
| BstF5I | GGATG | 2 cut(s) | 60, 520 |
| BstKTI | GATC | 3 cut(s) | 16, 285, 314 |
| BstMAI | GTCTC | 2 cut(s) | 143, 355 |
| BstMBI | GATC | 3 cut(s) | 13, 282, 311 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 110 |
| BstNI | CCWGG | 2 cut(s) | 116, 386 |
| BstSCI | CCNGG | 2 cut(s) | 114, 384 |
| BstSFI | CTRYAG | 1 cut(s) | 237 |
| BstSLI | GKGCMC | 1 cut(s) | 115 |
| BstV2I | GAAGAC | 1 cut(s) | 480 |
| BstX2I | RGATCY | 1 cut(s) | 311 |
| BstYI | RGATCY | 1 cut(s) | 311 |
| BsuRI | GGCC | 3 cut(s) | 42, 83, 636 |
| BtgI | CCRYGG | 2 cut(s) | 43, 50 |
| BtsCI | GGATG | 2 cut(s) | 60, 520 |
| BtsI | GCAGTG | 2 cut(s) | 246, 285 |
| BtsIMutI | CAGTG | 4 cut(s) | 115, 246, 285, 594 |
| BveI | ACCTGC | 1 cut(s) | 244 |
| Cac8I | GCNNGC | 2 cut(s) | 27, 302 |
| Cfr13I | GGNCC | 1 cut(s) | 47 |
| Csp6I | GTAC | 2 cut(s) | 188, 573 |
| CviAII | CATG | 2 cut(s) | 51, 61 |
| CviJI | RGCY | 9 cut(s) | 42, 83, 304, 325, 452, 463, 504, 570, 636 |
| CviKI_1 | RGCY | 9 cut(s) | 42, 83, 304, 325, 452, 463, 504, 570, 636 |
| CviQI | GTAC | 2 cut(s) | 188, 573 |
| DdeI | CTNAG | 4 cut(s) | 315, 459, 505, 564 |
| DpnI | GATC | 3 cut(s) | 15, 284, 313 |
| DpnII | GATC | 3 cut(s) | 13, 282, 311 |
| DraIII | CACNNNGTG | 1 cut(s) | 424 |
| EaeI | YGGCCR | 2 cut(s) | 40, 634 |
| Eco130I | CCWWGG | 1 cut(s) | 50 |
| Eco31I | GGTCTC | 1 cut(s) | 355 |
| Eco47I | GGWCC | 1 cut(s) | 47 |
| Eco57I | CTGAAG | 1 cut(s) | 474 |
| EcoRII | CCWGG | 2 cut(s) | 114, 384 |
| EcoT14I | CCWWGG | 1 cut(s) | 50 |
| ErhI | CCWWGG | 1 cut(s) | 50 |
| FaeI | CATG | 2 cut(s) | 54, 64 |
| FaiI | YATR | 8 cut(s) | 52, 62, 69, 253, 281, 338, 467, 604 |
| FatI | CATG | 2 cut(s) | 50, 60 |
| FbaI | TGATCA | 2 cut(s) | 13, 282 |
| FokI | GGATG | 2 cut(s) | 67, 507 |
| FspBI | CTAG | 2 cut(s) | 354, 627 |
| HaeIII | GGCC | 3 cut(s) | 42, 83, 636 |
| HapII | CCGG | 1 cut(s) | 309 |
| Hin1II | CATG | 2 cut(s) | 54, 64 |
| HincII | GTYRAC | 1 cut(s) | 588 |
| HindII | GTYRAC | 1 cut(s) | 588 |
| HinfI | GANTC | 4 cut(s) | 97, 135, 515, 584 |
| HpaII | CCGG | 1 cut(s) | 309 |
| HphI | GGTGA | 1 cut(s) | 176 |
| Hpy166II | GTNNAC | 1 cut(s) | 588 |
| Hpy188I | TCNGA | 3 cut(s) | 18, 318, 609 |
| Hpy188III | TCNNGA | 7 cut(s) | 132, 212, 230, 309, 411, 512, 563 |
| Hpy8I | GTNNAC | 1 cut(s) | 588 |
| HpyAV | CCTTC | 3 cut(s) | 73, 315, 537 |
| HpyCH4III | ACNGT | 1 cut(s) | 110 |
| HpyCH4IV | ACGT | 1 cut(s) | 140 |
| HpyCH4V | TGCA | 5 cut(s) | 25, 121, 239, 290, 594 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 110 |
| HpyF3I | CTNAG | 4 cut(s) | 315, 459, 505, 564 |
| HpySE526I | ACGT | 1 cut(s) | 140 |
| Hsp92II | CATG | 2 cut(s) | 54, 64 |
| Kpn2I | TCCGGA | 1 cut(s) | 308 |
| Ksp22I | TGATCA | 2 cut(s) | 13, 282 |
| Kzo9I | GATC | 3 cut(s) | 13, 282, 311 |
| LweI | GCATC | 2 cut(s) | 45, 205 |
| MaeI | CTAG | 2 cut(s) | 354, 627 |
| MaeII | ACGT | 1 cut(s) | 140 |
| MaeIII | GTNAC | 2 cut(s) | 141, 424 |
| MalI | GATC | 3 cut(s) | 15, 284, 313 |
| MboI | GATC | 3 cut(s) | 13, 282, 311 |
| MboII | GAAGA | 3 cut(s) | 399, 480, 489 |
| MflI | RGATCY | 1 cut(s) | 311 |
| MhlI | GDGCHC | 1 cut(s) | 115 |
| MlsI | TGGCCA | 2 cut(s) | 42, 636 |
| MluCI | AATT | 8 cut(s) | 6, 35, 377, 394, 532, 545, 553, 577 |
| MluNI | TGGCCA | 2 cut(s) | 42, 636 |
| MlyI | GAGTC | 1 cut(s) | 593 |
| MmeI | TCCRAC | 1 cut(s) | 632 |
| MnlI | CCTC | 7 cut(s) | 154, 160, 178, 336, 466, 534, 570 |
| Mox20I | TGGCCA | 2 cut(s) | 42, 636 |
| MroI | TCCGGA | 1 cut(s) | 308 |
| MroXI | GAANNNNTTC | 1 cut(s) | 394 |
| MscI | TGGCCA | 2 cut(s) | 42, 636 |
| Msp20I | TGGCCA | 2 cut(s) | 42, 636 |
| MspI | CCGG | 1 cut(s) | 309 |
| MspR9I | CCNGG | 2 cut(s) | 116, 386 |
| Mva1269I | GAATGC | 1 cut(s) | 178 |
| MvaI | CCWGG | 2 cut(s) | 116, 386 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 110 |
| NcoI | CCATGG | 1 cut(s) | 50 |
| NdeII | GATC | 3 cut(s) | 13, 282, 311 |
| NlaIII | CATG | 2 cut(s) | 54, 64 |
| NmuCI | GTSAC | 1 cut(s) | 424 |
| PctI | GAATGC | 1 cut(s) | 178 |
| PdmI | GAANNNNTTC | 1 cut(s) | 394 |
| PfeI | GAWTC | 3 cut(s) | 97, 135, 515 |
| PleI | GAGTC | 1 cut(s) | 592 |
| PpsI | GAGTC | 1 cut(s) | 592 |
| Psp6I | CCWGG | 2 cut(s) | 114, 384 |
| PspGI | CCWGG | 2 cut(s) | 114, 384 |
| PspPI | GGNCC | 1 cut(s) | 47 |
| PstI | CTGCAG | 1 cut(s) | 241 |
| PsuI | RGATCY | 1 cut(s) | 311 |
| RsaI | GTAC | 2 cut(s) | 189, 574 |
| RsaNI | GTAC | 2 cut(s) | 188, 573 |
| Sau3AI | GATC | 3 cut(s) | 13, 282, 311 |
| Sau96I | GGNCC | 1 cut(s) | 47 |
| SchI | GAGTC | 1 cut(s) | 593 |
| ScrFI | CCNGG | 2 cut(s) | 116, 386 |
| SduI | GDGCHC | 1 cut(s) | 115 |
| SetI | ASST | 8 cut(s) | 120, 143, 189, 238, 387, 458, 506, 572 |
| SfaNI | GCATC | 2 cut(s) | 45, 205 |
| SfcI | CTRYAG | 1 cut(s) | 237 |
| SinI | GGWCC | 1 cut(s) | 47 |
| SmlI | CTYRAG | 1 cut(s) | 146 |
| SmoI | CTYRAG | 1 cut(s) | 146 |
| Sse9I | AATT | 8 cut(s) | 6, 35, 377, 394, 532, 545, 553, 577 |
| SspMI | CTAG | 2 cut(s) | 354, 627 |
| StyD4I | CCNGG | 2 cut(s) | 114, 384 |
| StyI | CCWWGG | 1 cut(s) | 50 |
| TaaI | ACNGT | 1 cut(s) | 110 |
| TaiI | ACGT | 1 cut(s) | 143 |
| TaqI | TCGA | 1 cut(s) | 213 |
| TasI | AATT | 8 cut(s) | 6, 35, 377, 394, 532, 545, 553, 577 |
| TatI | WGTACW | 1 cut(s) | 572 |
| TfiI | GAWTC | 3 cut(s) | 97, 135, 515 |
| TscAI | CASTG | 4 cut(s) | 115, 246, 292, 601 |
| TseFI | GTSAC | 1 cut(s) | 424 |
| Tsp45I | GTSAC | 1 cut(s) | 424 |
| TspGWI | ACGGA | 1 cut(s) | 60 |
| TspRI | CASTG | 4 cut(s) | 115, 246, 292, 601 |
| VpaK11BI | GGWCC | 1 cut(s) | 47 |
| XapI | RAATTY | 4 cut(s) | 377, 394, 545, 577 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 122, 382 |
| XmnI | GAANNNNTTC | 1 cut(s) | 394 |
| XspI | CTAG | 2 cut(s) | 354, 627 |