MD16G1251300.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017135
Physical Location & Seq
Reverse (-)
28111484 .. 28112857
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1251300.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGCAGGCTTATTTGCAAAATCTGCTTCCCTCTCTCTTATTAATCTTGTTATTTTCTTATATTTATCATGCTTAACCACCAATCTCACGGCCTCCACGACCACAACTTCCGCCGTCAAAACACCAAGCCGTTTGGCCACAAAACTCATTCACCGTGATTCGGCTCTCTCTCCGTACTACAATCCTAATGCAACCATCAAAGACCGTGCTATAAGCACATTGAAACGCTCCAGGGCACGCTTAGCATGCTTGGGAGCAAAGGCCGGTTTTTCCAGTGACGATGACACCCGTGCTGGTCTTGTTGCCGAGGACAGTGGAGCACAATTTATGGCCAATTTCTCAATCGGTGAGCCACCAGTTCCACAGCTCTTAACCATAGACACAGGGAGCAACCTCTTGTGGGTTCAATGCCTTCCTTGCACCAAGTGCTTTGAACAAACAAGTCCGATTTTTGACCCTTCCAAGTCCTCTACATACACCCCATTATCATGCTCATCCCCATATTGCACAATTTCCCCTAGTGACAAATGTGACCCCTCAAACAATTGCAAATTCTCTCACACATACTTTGATAGCACCAAAGTAGCTGGACTTATTGGGACCGAAATTTTCACTTTTGAGACGTCCGACGAAGGCATTAGCACTGTCCCGAATGTTGTTTTTGGTTGTGCCCAGGACAATGATGGTTACAACGGGCAGCCTAGTGGAATAATTGGTCTAGGCCCTTCAAACATATCTTTGGCAAACCACATGGGGTCAAAATTCTCTTATTGCATCGGTAGCATAAGGGATCCTAACTACCCTCATAACCAGTTAGTCTTAGGCGATGGTGCAAAGATAGAAGGCACTTCAACTCCACTAGAAGTCTACAATGAACTTTACTATCTAACCCTAGAGAGCATCAGCCTCGCGGAAAGGCGGCTTGAGATCGATCCGGAAATCTTCAAGAGAACAGAATCAGGCTCGGGTGGAACAGTCATCGATTCCGGAACAACAATATCCTTCTTAGCTACAGGTGCATACTATGTGCTTAGAGATGAAGTTCGGAAGTTGCTTGATGGAAGATTGGAAAAAGTGGAAGAGCCTGGTGTTCCTTCAGGTTTGTGCTACAAGGGGAATATAAGGGAAGATTTAACAGGGTTTCCAGATGTTGTGTTGAACTTTGCAGGTGGGGCTGAAGTAGGGTTAGACGTAGAGAGCGCTTTTCAAGATTATGGTGAGGGTGAGTTTTGCATGGCAGTGCAACTGTCTCCTGAGGGCAGTGACTTGAACGTTGTTGGGATTATGGCGCAGCAGCATTACAATGTTGCCTATGATCTTGCTGCAAGCCAGGTTTATTTCCAGAGGATTGATTGTCAACTTCTTGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

458

Amino Acids

49.24

Weight (kDa)

4.66

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 108 - 160 4.8e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 109 - 276 4e-44 Xylanase inhibitor N-terminal
TAXi_C PF14541 294 - 448 1.9e-27 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23945 AT4G30030 AT4G30040
fragaria_vesca FvH4_4g07480
malus_domestica MD16G1251100.v1.1 MD16G1251300.v1.1
prunus_persica Prupe.1G077600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399921
rosa_rugosa Rorug04G0020200
rosa_samantha Rh4BG094700 Rh4CG106400 Rh4DG091200
rosa_wichuraiana Rw4G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1157
AatII GACGTC 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 1157
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 911
AccIII TCCGGA 2 cut(s) 934, 986
AciI CCGC 3 cut(s) 111, 911, 919
AclI AACGTT 1 cut(s) 1272
AclWI GGATC 3 cut(s) 785, 798, 926
AcoI YGGCCR 2 cut(s) 135, 330
AcsI RAATTY 3 cut(s) 551, 606, 761
AcuI CTGAAG 2 cut(s) 1080, 1197
AcyI GRCGYC 1 cut(s) 623
AdeI CACNNNGTG 1 cut(s) 426
AfaI GTAC 1 cut(s) 176
AfeI AGCGCT 1 cut(s) 1201
AfiI CCNNNNNNNGG 2 cut(s) 160, 400
AgsI TTSAA 9 cut(s) 223, 407, 434, 729, 852, 946, 1159, 1208, 1270
AjnI CCWGG 4 cut(s) 230, 672, 1084, 1329
AluBI AGCT 3 cut(s) 367, 587, 1010
AluI AGCT 3 cut(s) 367, 587, 1010
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 2 cut(s) 614, 1254
AlwI GGATC 3 cut(s) 785, 798, 926
Ama87I CYCGRG 1 cut(s) 964
Aor13HI TCCGGA 2 cut(s) 934, 986
Aor51HI AGCGCT 1 cut(s) 1201
AoxI GGCC 5 cut(s) 90, 135, 261, 330, 721
ApeKI GCWGC 4 cut(s) 697, 1291, 1294, 1322
ApoI RAATTY 3 cut(s) 551, 606, 761
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 2 cut(s) 1202, 1291
AspS9I GGNCC 2 cut(s) 600, 722
AsuHPI GGTGA 4 cut(s) 143, 359, 1229, 1235
AvaI CYCGRG 1 cut(s) 964
AvaII GGWCC 1 cut(s) 600
AxyI CCTNAGG 1 cut(s) 1254
BaeGI GKGCMC 2 cut(s) 238, 673
BalI TGGCCA 2 cut(s) 137, 332
BamHI GGATCC 1 cut(s) 790
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 4 cut(s) 709, 1303, 1306, 1309
BccI CCATC 4 cut(s) 203, 677, 821, 1052
BceAI ACGGC 3 cut(s) 98, 105, 114
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 4 cut(s) 232, 674, 1086, 1331
BcoDI GTCTC 2 cut(s) 614, 1254
BfaI CTAG 5 cut(s) 519, 702, 719, 860, 893
BfmI CTRYAG 1 cut(s) 1011
BfoI RGCGCY 1 cut(s) 1203
BfuAI ACCTGC 1 cut(s) 1157
BisI GCNGC 5 cut(s) 698, 920, 1292, 1295, 1323
BlpI GCTNAGC 1 cut(s) 241
BlsI GCNGC 5 cut(s) 699, 921, 1293, 1296, 1324
Bme1390I CCNGG 4 cut(s) 232, 674, 1086, 1331
Bme18I GGWCC 1 cut(s) 600
BmeT110I CYCGRG 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 600, 722
BmiI GGNNCC 2 cut(s) 601, 792
BmrFI CCNGG 4 cut(s) 232, 674, 1086, 1331
BmsI GCATC 2 cut(s) 783, 909
BpmI CTGGAG 1 cut(s) 214
Bpu1102I GCTNAGC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 944
Bsa29I ATCGAT 2 cut(s) 930, 981
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 3 cut(s) 231, 306, 672
BsaWI WCCGGW 2 cut(s) 934, 986
BsaXI ACNNNNNCTCC 2 cut(s) 838, 868
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 400
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 273, 356, 811
Bse21I CCTNAGG 1 cut(s) 1254
BseAI TCCGGA 2 cut(s) 934, 986
BseBI CCWGG 4 cut(s) 232, 674, 1086, 1331
BseCI ATCGAT 2 cut(s) 930, 981
BseDI CCNNGG 3 cut(s) 231, 306, 672
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 2 cut(s) 160, 400
BseMII CTCAG 1 cut(s) 1245
BseNI ACTGG 3 cut(s) 273, 356, 811
BseSI GKGCMC 2 cut(s) 238, 673
BseXI GCAGC 4 cut(s) 709, 1303, 1306, 1309
Bsh1236I CGCG 1 cut(s) 911
BshFI GGCC 5 cut(s) 92, 137, 263, 332, 723
BshVI ATCGAT 2 cut(s) 930, 981
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 964
BsiSI CCGG 3 cut(s) 264, 935, 987
BslFI GGGAC 2 cut(s) 613, 632
BslI CCNNNNNNNGG 2 cut(s) 160, 400
BsmAI GTCTC 2 cut(s) 614, 1254
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 613, 632
BsnI GGCC 5 cut(s) 92, 137, 263, 332, 723
BsoBI CYCGRG 1 cut(s) 964
Bsp1286I GDGCHC 3 cut(s) 238, 322, 673
Bsp13I TCCGGA 2 cut(s) 934, 986
Bsp143I GATC 4 cut(s) 790, 927, 931, 1315
Bsp1720I GCTNAGC 1 cut(s) 241
BspACI CCGC 3 cut(s) 111, 911, 919
BspANI GGCC 5 cut(s) 92, 137, 263, 332, 723
BspCNI CTCAG 1 cut(s) 1246
BspDI ATCGAT 2 cut(s) 930, 981
BspEI TCCGGA 2 cut(s) 934, 986
BspFNI CGCG 1 cut(s) 911
BspLI GGNNCC 2 cut(s) 601, 792
BspMI ACCTGC 1 cut(s) 1157
BspPI GGATC 3 cut(s) 785, 798, 926
BspQI GCTCTTC 1 cut(s) 1074
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 273, 356, 811
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 3 cut(s) 231, 306, 672
BssMI GATC 4 cut(s) 790, 927, 931, 1315
BssNI GRCGYC 1 cut(s) 623
Bst2UI CCWGG 4 cut(s) 232, 674, 1086, 1331
Bst4CI ACNGT 6 cut(s) 155, 206, 314, 646, 976, 1248
Bst6I CTCTTC 1 cut(s) 1074
BstACI GRCGYC 1 cut(s) 623
BstAPI GCANNNNNTGC 2 cut(s) 23, 426
BstC8I GCNNGC 4 cut(s) 7, 238, 247, 1327
BstDEI CTNAG 5 cut(s) 241, 820, 1006, 1031, 1254
BstF5I GGATG 1 cut(s) 494
BstFNI CGCG 1 cut(s) 911
BstH2I RGCGCY 1 cut(s) 1203
BstHHI GCGC 2 cut(s) 1202, 1291
BstKTI GATC 4 cut(s) 793, 930, 934, 1318
BstMAI GTCTC 2 cut(s) 614, 1254
BstMBI GATC 4 cut(s) 790, 927, 931, 1315
BstMWI GCNNNNNNNGC 8 cut(s) 23, 242, 246, 417, 426, 780, 1016, 1172
BstNI CCWGG 4 cut(s) 232, 674, 1086, 1331
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 4 cut(s) 230, 672, 1084, 1329
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 2 cut(s) 238, 673
BstUI CGCG 1 cut(s) 911
BstV1I GCAGC 4 cut(s) 709, 1303, 1306, 1309
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
Bsu15I ATCGAT 2 cut(s) 930, 981
Bsu36I CCTNAGG 1 cut(s) 1254
BsuRI GGCC 5 cut(s) 92, 137, 263, 332, 723
BsuTUI ATCGAT 2 cut(s) 930, 981
BtgZI GCGATG 1 cut(s) 840
BtsCI GGATG 1 cut(s) 494
BtsI GCAGTG 2 cut(s) 1245, 1267
BtsIMutI CAGTG 5 cut(s) 280, 319, 642, 1245, 1267
BveI ACCTGC 1 cut(s) 1157
Cac8I GCNNGC 4 cut(s) 7, 238, 247, 1327
CfoI GCGC 2 cut(s) 1202, 1291
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 2 cut(s) 600, 722
ClaI ATCGAT 2 cut(s) 930, 981
Csp6I GTAC 1 cut(s) 175
CviAII CATG 5 cut(s) 69, 246, 489, 751, 1234
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 5 cut(s) 241, 820, 1006, 1031, 1254
DpnI GATC 4 cut(s) 792, 929, 933, 1317
DpnII GATC 4 cut(s) 790, 927, 931, 1315
DraIII CACNNNGTG 1 cut(s) 426
EaeI YGGCCR 2 cut(s) 135, 330
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
EciI GGCGGA 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 600
Eco47III AGCGCT 1 cut(s) 1201
Eco57I CTGAAG 2 cut(s) 1080, 1197
Eco81I CCTNAGG 1 cut(s) 1254
Eco88I CYCGRG 1 cut(s) 964
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 4 cut(s) 230, 672, 1084, 1329
Esp3I CGTCTC 1 cut(s) 614
FaeI CATG 5 cut(s) 72, 249, 492, 754, 1237
FaqI GGGAC 2 cut(s) 613, 632
FatI CATG 5 cut(s) 68, 245, 488, 750, 1233
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 5 cut(s) 698, 920, 1292, 1295, 1323
FokI GGATG 1 cut(s) 481
Fsp4HI GCNGC 5 cut(s) 698, 920, 1292, 1295, 1323
FspBI CTAG 5 cut(s) 519, 702, 719, 860, 893
GlaI GCGC 2 cut(s) 1201, 1290
GluI GCNGC 5 cut(s) 698, 920, 1292, 1295, 1323
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 1203
HaeIII GGCC 5 cut(s) 92, 137, 263, 332, 723
HapII CCGG 3 cut(s) 264, 935, 987
HhaI GCGC 2 cut(s) 1202, 1291
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 5 cut(s) 72, 249, 492, 754, 1237
Hin6I GCGC 2 cut(s) 1200, 1289
HinP1I GCGC 2 cut(s) 1200, 1289
HincII GTYRAC 1 cut(s) 1358
HindII GTYRAC 1 cut(s) 1358
HinfI GANTC 3 cut(s) 158, 956, 983
HpaII CCGG 3 cut(s) 264, 935, 987
HphI GGTGA 4 cut(s) 143, 359, 1229, 1235
Hpy166II GTNNAC 2 cut(s) 868, 1358
Hpy188I TCNGA 3 cut(s) 447, 628, 1047
Hpy188III TCNNGA 9 cut(s) 649, 935, 946, 987, 1145, 1208, 1253, 1342, 1364
Hpy8I GTNNAC 2 cut(s) 868, 1358
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 7 cut(s) 422, 468, 626, 735, 836, 1012, 1104
HpyCH4III ACNGT 6 cut(s) 155, 206, 314, 646, 976, 1248
HpyCH4IV ACGT 3 cut(s) 623, 1191, 1272
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 242, 246, 417, 426, 780, 1016, 1172
HpyF3I CTNAG 5 cut(s) 241, 820, 1006, 1031, 1254
HpySE526I ACGT 3 cut(s) 623, 1191, 1272
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 5 cut(s) 72, 249, 492, 754, 1237
HspAI GCGC 2 cut(s) 1200, 1289
Kpn2I TCCGGA 2 cut(s) 934, 986
Kzo9I GATC 4 cut(s) 790, 927, 931, 1315
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 4 cut(s) 233, 254, 317, 387
Lsp1109I GCAGC 4 cut(s) 709, 1303, 1306, 1309
LweI GCATC 2 cut(s) 783, 909
MaeI CTAG 5 cut(s) 519, 702, 719, 860, 893
MaeII ACGT 3 cut(s) 623, 1191, 1272
MaeIII GTNAC 5 cut(s) 275, 521, 530, 686, 1262
MalI GATC 4 cut(s) 792, 929, 933, 1317
MboI GATC 4 cut(s) 790, 927, 931, 1315
MboII GAAGA 4 cut(s) 934, 1074, 1091, 1139
MfeI CAATTG 1 cut(s) 544
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 3 cut(s) 238, 322, 673
MlsI TGGCCA 2 cut(s) 137, 332
MluCI AATT 8 cut(s) 323, 334, 510, 544, 551, 606, 711, 761
MluNI TGGCCA 2 cut(s) 137, 332
MmeI TCCRAC 1 cut(s) 651
Mox20I TGGCCA 2 cut(s) 137, 332
MroI TCCGGA 2 cut(s) 934, 986
MscI TGGCCA 2 cut(s) 137, 332
MseI TTAA 5 cut(s) 42, 74, 371, 1133, 1372
MslI CAYNNNNRTG 3 cut(s) 487, 1238, 1302
Msp20I TGGCCA 2 cut(s) 137, 332
MspI CCGG 3 cut(s) 264, 935, 987
MspR9I CCNGG 4 cut(s) 232, 674, 1086, 1331
MunI CAATTG 1 cut(s) 544
MvaI CCWGG 4 cut(s) 232, 674, 1086, 1331
MvnI CGCG 1 cut(s) 911
MwoI GCNNNNNNNGC 8 cut(s) 23, 242, 246, 417, 426, 780, 1016, 1172
NdeII GATC 4 cut(s) 790, 927, 931, 1315
NlaIII CATG 5 cut(s) 72, 249, 492, 754, 1237
NlaIV GGNNCC 2 cut(s) 601, 792
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 4 cut(s) 275, 521, 530, 1262
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 1157
PciSI GCTCTTC 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PfeI GAWTC 3 cut(s) 158, 956, 983
PkrI GCNGC 5 cut(s) 699, 921, 1293, 1296, 1324
PshBI ATTAAT 1 cut(s) 42
Psp1406I AACGTT 1 cut(s) 1272
Psp6I CCWGG 4 cut(s) 230, 672, 1084, 1329
PspGI CCWGG 4 cut(s) 230, 672, 1084, 1329
PspN4I GGNNCC 2 cut(s) 601, 792
PspPI GGNCC 2 cut(s) 600, 722
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 3 cut(s) 487, 1238, 1302
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 5 cut(s) 42, 74, 371, 1133, 1372
SatI GCNGC 5 cut(s) 698, 920, 1292, 1295, 1323
Sau3AI GATC 4 cut(s) 790, 927, 931, 1315
Sau96I GGNCC 2 cut(s) 600, 722
ScrFI CCNGG 4 cut(s) 232, 674, 1086, 1331
SduI GDGCHC 3 cut(s) 238, 322, 673
SfaNI GCATC 2 cut(s) 783, 909
SfcI CTRYAG 1 cut(s) 1011
SinI GGWCC 1 cut(s) 600
SmiMI CAYNNNNRTG 3 cut(s) 487, 1238, 1302
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
SphI GCATGC 1 cut(s) 249
Sse9I AATT 8 cut(s) 323, 334, 510, 544, 551, 606, 711, 761
SsiI CCGC 3 cut(s) 111, 911, 919
SspMI CTAG 5 cut(s) 519, 702, 719, 860, 893
StyD4I CCNGG 4 cut(s) 230, 672, 1084, 1329
TaaI ACNGT 6 cut(s) 155, 206, 314, 646, 976, 1248
TaiI ACGT 3 cut(s) 626, 1194, 1275
TaqI TCGA 2 cut(s) 930, 981
TaqII GACCGA 1 cut(s) 617
TasI AATT 8 cut(s) 323, 334, 510, 544, 551, 606, 711, 761
TauI GCSGC 1 cut(s) 922
TfiI GAWTC 3 cut(s) 158, 956, 983
Tru1I TTAA 5 cut(s) 42, 74, 371, 1133, 1372
Tru9I TTAA 5 cut(s) 42, 74, 371, 1133, 1372
TscAI CASTG 5 cut(s) 280, 319, 649, 1245, 1267
TseFI GTSAC 4 cut(s) 275, 521, 530, 1262
TseI GCWGC 4 cut(s) 697, 1291, 1294, 1322
Tsp45I GTSAC 4 cut(s) 275, 521, 530, 1262
TspDTI ATGAA 2 cut(s) 888, 1053
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 5 cut(s) 280, 319, 649, 1245, 1267
VpaK11BI GGWCC 1 cut(s) 600
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 3 cut(s) 551, 606, 761
XceI RCATGY 1 cut(s) 249
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 5 cut(s) 519, 702, 719, 860, 893
ZraI GACGTC 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.