Rh4DG091200

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017135
Physical Location & Seq
Reverse (-)
13747516 .. 13830984
83469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG091200.1

Sequence Viewer

Length: 2148 bp
ATGAAGTTTTATGGGACTTACTTGTTTGTCTTATTCAGTCTTCTCCTACTATCCTCCTATGTTAATGGTGACCATGTTTTTTCCGATAGTTCAGCAAATTATGATGATGTCTTAAGGCTTGTGCCTAAGGGTCCTAATCCTCGAGAGTCTCCACCATTGGATGTCTTCAGGCGTGTGCCTACGGGTCCCAACCCTCGGCAGTCACCACCGGTGCAAGATTTCGAGGATGAGGCCTTTGATAGTTCATCCAACCATGATGATGTCTTAAGGCTTGTCCCCAAAGGTCCGAATCCTCAGCAGCCTCCACCACTGAATGTCTTAACGCGTGTGCCGCCTTCAGGCCCGAATCCTCGAGAGTCTCCACTGTTGGATGTCTTAAGGCTTGTGCCTAAAGGTCCGAATCCTCAGCAGCCTCCACCATTGAATGTCTTAAAGCATGTGCCGCCTTCAGGTCCTAATCGTCAAGAGTCTCCACCATTGAGTCCCTTCCTAATAAGTGGTAACGGCTATGAAATCAAGTACATACCTTGTAAGGTGGTCAATCCATTATGTTGGGAAGCGATTAGGAAAACCATCCAAAAGAAGGTAAAGACTTCCATATATACCTGTTCCATAGAAGACCTTCCAGAGTTCCAGTACGTAGCCATGGCAACCGCAGTCGCAGAACTTGTTAGGCTCTCTCTTATTACTTTCCTTTTAACCTCACACAATTTATGCTTAACCGCCAACCCTGCCACAACCTCCACCGTCACAAAACCAAAGCGTTTTGTCACAAAACTCATTCACCATGATTCGGCTTTGTCTCCTTACTATAATCCTAATGCAAGCATTAGAGATATTGTTTCACGCTCAGTGAAAAGCTCCGCGGCACGCTTCACATACCTAAAAGCAAAGAGTACTAATGCTTTTGTGATGGACGATACACGTGCTGGTGTTGTTCCGGAGAAGTCTGCAACAGAGTTTATGGCAAATTTCTCATTTGGAGAGCCACCAGTTCCACAGCTTTTGACAATGGATACCGGCAGCGACTTATTGTGGGTTCAATGCCTGCCTTGCACCAAGTGTTTTAAACAACCCAATCCGATTTTTGATCCTTCCAAGTCCTCCACATACAAAACCTTGCCATGCGCATCTCCCGATTGTATTCTGTCGAGCCCTGCTGATAAGTGTGATTTCTCAAACAATTGCAAATTCCTTCAGAAGTACCTTGATGGCACCGCGGCGGCAGGACTTATCGCGACCGAAAAGCTCACCTTCGAGACATCTGACGAAGGGCTTAGCCCCATCTCCGATGCAGTATTTGGCTGTGCACATGACAATGTTGCTGCGTACGATGGACAAATAAGTGGAGTACTTGGTCTTGGCCCTTCAAAAATATCTTTGACAACCAAGTTAGGCTCCAAGTTTTCTTATTGCATTGGAAGCATAAGGGACCCTAACTACCCTCACAACCAATTGATCTTCGGTGAGGGTACAAAGATAGAAGGGGCAGCAACCCCCCTTCAACTGTACAATGAACTTTACTATCTAACCCTAGAGGGCATCAGCCTCGGTCAGTCCCGCCTCCAAATTGATCCAAAAGTATTCACCAGATCACCATCAGGAACTGGTGGAACGGTCATCGACTCCGGAACTACACTAAGCTTCGTGGTTAAAAGTGCATATGATGTACTCAGTGCTGAAGTGCAAAAATTAGCAAACGGAATATTAGAACGTGTCCAAGATCCTGATACGCCCACAGCTCTTTGCTACAAGGGGACAATAGACCGAGACCTTGTTGGTTTTCCAGGTGCAACGTTTCACCTGGCAGGAGGGGTTGATTTGTTTCTGGACAAAACGAGCCTCTTCGAAGTTACTGGTGAGAATGAGTTCTGTTTAGCTGTGCAAATGCCAGAGGGCGAGGGTGAAGATTTGAATATTATTGGAATTCTGGCACAGCAAAATTATAATGTTGCATATGACCTTGCCGCAAAGAAGGTTTATTTCCAGCAGACAGCAACCTTCACCAAAATGCAATCAAACCCAGTGGTCCCAGTCCAGGCTGCAAAAAGAAATGGCATGCATAAGGACTGCCATCTTCTGAGTTGGAAACATGACAAGGAATTTTCACGAAACAAATGGTATAGAAAGGCATTTTACCTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

715

Amino Acids

78.49

Weight (kDa)

6.86

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 321 - 489 1.1e-43 Xylanase inhibitor N-terminal
TAXi_C PF14541 508 - 663 5.5e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23945 AT4G30030 AT4G30040
fragaria_vesca FvH4_4g07480
malus_domestica MD16G1251100.v1.1 MD16G1251300.v1.1
prunus_persica Prupe.1G077600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399921
rosa_rugosa Rorug04G0020200
rosa_samantha Rh4BG094700 Rh4CG106400 Rh4DG091200
rosa_wichuraiana Rw4G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1947
Acc16I TGCGCA 1 cut(s) 1129
AccB1I GGYRCC 1 cut(s) 1214
AccII CGCG 4 cut(s) 325, 866, 1220, 1238
AccIII TCCGGA 2 cut(s) 940, 1628
AclI AACGTT 1 cut(s) 1796
AclWI GGATC 3 cut(s) 1085, 1568, 1718
AcsI RAATTY 4 cut(s) 970, 1190, 1926, 2102
AcuI CTGAAG 5 cut(s) 151, 321, 432, 1181, 1701
AcvI CACGTG 1 cut(s) 926
AdeI CACNNNGTG 1 cut(s) 1062
AfaI GTAC 9 cut(s) 521, 638, 898, 1205, 1331, 1353, 1474, 1511, 1671
AfiI CCNNNNNNNGG 6 cut(s) 195, 338, 449, 583, 793, 2038
AflII CTTAAG 3 cut(s) 112, 265, 376
AflIII ACRYGT 3 cut(s) 323, 923, 1714
AgeI ACCGGT 1 cut(s) 208
AgsI TTSAA 5 cut(s) 424, 1043, 1371, 1505, 1915
AjnI CCWGG 4 cut(s) 1786, 1803, 2037, 2139
AluBI AGCT 6 cut(s) 861, 1003, 1249, 1644, 1742, 1880
AluI AGCT 6 cut(s) 861, 1003, 1249, 1644, 1742, 1880
Alw21I GWGCWC 1 cut(s) 1312
Alw26I GTCTC 6 cut(s) 153, 363, 474, 807, 1253, 1764
Alw44I GTGCAC 1 cut(s) 1308
AlwI GGATC 3 cut(s) 1085, 1568, 1718
AlwNI CAGNNNCTG 1 cut(s) 1607
Ama87I CYCGRG 2 cut(s) 141, 351
Aor13HI TCCGGA 2 cut(s) 940, 1628
AoxI GGCC 3 cut(s) 231, 340, 1363
ApaLI GTGCAC 1 cut(s) 1308
ApeKI GCWGC 6 cut(s) 298, 409, 1023, 1325, 1490, 2042
ApoI RAATTY 4 cut(s) 970, 1190, 1926, 2102
AsiGI ACCGGT 1 cut(s) 208
Asp700I GAANNNNTTC 2 cut(s) 621, 1868
AspLEI GCGC 1 cut(s) 1130
AspS9I GGNCC 9 cut(s) 131, 185, 284, 341, 395, 452, 1364, 1432, 2029
AsuII TTCGAA 1 cut(s) 1848
AvaI CYCGRG 2 cut(s) 141, 351
AvaII GGWCC 7 cut(s) 131, 185, 284, 395, 452, 1432, 2029
AxyI CCTNAGG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 1312
BanI GGYRCC 1 cut(s) 1214
BanII GRGCYC 1 cut(s) 1157
BbrPI CACGTG 1 cut(s) 926
BbsI GAAGAC 3 cut(s) 32, 157, 624
Bbv12I GWGCWC 1 cut(s) 1312
BbvCI CCTCAGC 2 cut(s) 294, 405
BbvI GCAGC 6 cut(s) 310, 421, 1035, 1312, 1502, 2029
BccI CCATC 7 cut(s) 581, 907, 1205, 1292, 1328, 1606, 2082
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 4 cut(s) 908, 942, 1531, 1565
BciT130I CCWGG 4 cut(s) 1788, 1805, 2039, 2141
BciVI GTATCC 1 cut(s) 1009
BcoDI GTCTC 6 cut(s) 153, 363, 474, 807, 1253, 1764
BfaI CTAG 1 cut(s) 1535
BfrI CTTAAG 3 cut(s) 112, 265, 376
BfuI GTATCC 1 cut(s) 1009
BlpI GCTNAGC 1 cut(s) 1277
BmcAI AGTACT 2 cut(s) 898, 1353
Bme1390I CCNGG 4 cut(s) 1788, 1805, 2039, 2141
Bme18I GGWCC 7 cut(s) 131, 185, 284, 395, 452, 1432, 2029
BmeT110I CYCGRG 2 cut(s) 141, 351
BmgT120I GGNCC 9 cut(s) 131, 185, 284, 341, 395, 452, 1364, 1432, 2029
BmiI GGNNCC 8 cut(s) 132, 186, 187, 1216, 1399, 1433, 1434, 2031
BmrFI CCNGG 4 cut(s) 1788, 1805, 2039, 2141
BmrI ACTGGG 2 cut(s) 2018, 2027
BmsI GCATC 3 cut(s) 1139, 1282, 1551
BmuI ACTGGG 2 cut(s) 2018, 2027
BpiI GAAGAC 3 cut(s) 32, 157, 624
Bpu10I CCTNAGC 2 cut(s) 294, 405
Bpu1102I GCTNAGC 1 cut(s) 1277
Bpu14I TTCGAA 1 cut(s) 1848
BsaAI YACGTR 2 cut(s) 640, 926
BsaBI GATNNNNATC 1 cut(s) 1597
BsaI GGTCTC 1 cut(s) 1764
BsaJI CCNNGG 5 cut(s) 194, 645, 864, 1218, 1549
BsaWI WCCGGW 3 cut(s) 208, 940, 1628
Bsc4I CCNNNNNNNGG 6 cut(s) 195, 338, 449, 583, 793, 2038
Bse118I RCCGGY 2 cut(s) 208, 1019
Bse1I ACTGG 6 cut(s) 634, 992, 1612, 1861, 2024, 2033
Bse21I CCTNAGG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 1597
BseAI TCCGGA 2 cut(s) 940, 1628
BseBI CCWGG 4 cut(s) 1788, 1805, 2039, 2141
BseDI CCNNGG 5 cut(s) 194, 645, 864, 1218, 1549
BseGI GGATG 5 cut(s) 166, 232, 245, 376, 573
BseJI GATNNNNATC 1 cut(s) 1597
BseLI CCNNNNNNNGG 6 cut(s) 195, 338, 449, 583, 793, 2038
BseMII CTCAG 5 cut(s) 308, 419, 864, 1687, 2072
BseNI ACTGG 6 cut(s) 634, 992, 1612, 1861, 2024, 2033
BseSI GKGCMC 1 cut(s) 1312
BseXI GCAGC 6 cut(s) 310, 421, 1035, 1312, 1502, 2029
Bsh1236I CGCG 4 cut(s) 325, 866, 1220, 1238
Bsh1285I CGRYCG 1 cut(s) 1242
BshFI GGCC 3 cut(s) 233, 342, 1365
BshNI GGYRCC 1 cut(s) 1214
BshTI ACCGGT 1 cut(s) 208
BsiEI CGRYCG 1 cut(s) 1242
BsiHKAI GWGCWC 1 cut(s) 1312
BsiHKCI CYCGRG 2 cut(s) 141, 351
BsiSI CCGG 4 cut(s) 209, 941, 1020, 1629
BsiWI CGTACG 1 cut(s) 1329
BslFI GGGAC 8 cut(s) 28, 171, 260, 468, 1445, 1543, 1771, 2015
BslI CCNNNNNNNGG 6 cut(s) 195, 338, 449, 583, 793, 2038
BsmAI GTCTC 6 cut(s) 153, 363, 474, 807, 1253, 1764
BsmFI GGGAC 8 cut(s) 28, 171, 260, 468, 1445, 1543, 1771, 2015
BsnI GGCC 3 cut(s) 233, 342, 1365
Bso31I GGTCTC 1 cut(s) 1764
BsoBI CYCGRG 2 cut(s) 141, 351
Bsp119I TTCGAA 1 cut(s) 1848
Bsp1286I GDGCHC 2 cut(s) 1157, 1312
Bsp13I TCCGGA 2 cut(s) 940, 1628
Bsp1407I TGTACA 1 cut(s) 1509
Bsp143I GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
Bsp1720I GCTNAGC 1 cut(s) 1277
Bsp19I CCATGG 1 cut(s) 645
Bsp68I TCGCGA 1 cut(s) 1238
BspANI GGCC 3 cut(s) 233, 342, 1365
BspCNI CTCAG 5 cut(s) 307, 418, 863, 1686, 2073
BspEI TCCGGA 2 cut(s) 940, 1628
BspFNI CGCG 4 cut(s) 325, 866, 1220, 1238
BspLI GGNNCC 8 cut(s) 132, 186, 187, 1216, 1399, 1433, 1434, 2031
BspPI GGATC 3 cut(s) 1085, 1568, 1718
BspT104I TTCGAA 1 cut(s) 1848
BspT107I GGYRCC 1 cut(s) 1214
BspTI CTTAAG 3 cut(s) 112, 265, 376
BspTNI GGTCTC 1 cut(s) 1764
BsrFI RCCGGY 2 cut(s) 208, 1019
BsrGI TGTACA 1 cut(s) 1509
BsrI ACTGG 6 cut(s) 634, 992, 1612, 1861, 2024, 2033
BssAI RCCGGY 2 cut(s) 208, 1019
BssECI CCNNGG 5 cut(s) 194, 645, 864, 1218, 1549
BssMI GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
BssT1I CCWWGG 1 cut(s) 645
Bst2UI CCWGG 4 cut(s) 1788, 1805, 2039, 2141
Bst4CI ACNGT 4 cut(s) 366, 748, 1509, 1618
Bst6I CTCTTC 1 cut(s) 1850
BstAFI CTTAAG 3 cut(s) 112, 265, 376
BstAUI TGTACA 1 cut(s) 1509
BstBAI YACGTR 2 cut(s) 640, 926
BstBI TTCGAA 1 cut(s) 1848
BstC8I GCNNGC 4 cut(s) 826, 871, 1049, 2060
BstDEI CTNAG 8 cut(s) 126, 294, 405, 850, 1277, 1640, 1673, 2081
BstDSI CCRYGG 3 cut(s) 645, 864, 1218
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 5 cut(s) 166, 232, 245, 376, 573
BstFNI CGCG 4 cut(s) 325, 866, 1220, 1238
BstHHI GCGC 1 cut(s) 1130
BstKTI GATC 5 cut(s) 1093, 1461, 1576, 1595, 1726
BstMAI GTCTC 6 cut(s) 153, 363, 474, 807, 1253, 1764
BstMBI GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
BstMCI CGRYCG 1 cut(s) 1242
BstMWI GCNNNNNNNGC 5 cut(s) 331, 442, 731, 1053, 1422
BstNI CCWGG 4 cut(s) 1788, 1805, 2039, 2141
BstNSI RCATGY 2 cut(s) 440, 2062
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 4 cut(s) 1786, 1803, 2037, 2139
BstSLI GKGCMC 1 cut(s) 1312
BstSNI TACGTA 1 cut(s) 640
BstUI CGCG 4 cut(s) 325, 866, 1220, 1238
BstV1I GCAGC 6 cut(s) 310, 421, 1035, 1312, 1502, 2029
BstV2I GAAGAC 3 cut(s) 32, 157, 624
BstX2I RGATCY 1 cut(s) 1723
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 1 cut(s) 1723
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 233, 342, 1365
BtgI CCRYGG 3 cut(s) 645, 864, 1218
BtsCI GGATG 5 cut(s) 166, 232, 245, 376, 573
BtsIMutI CAGTG 5 cut(s) 308, 362, 858, 1681, 2031
BtuMI TCGCGA 1 cut(s) 1238
Cac8I GCNNGC 4 cut(s) 826, 871, 1049, 2060
CaiI CAGNNNCTG 1 cut(s) 1607
CfoI GCGC 1 cut(s) 1130
Cfr10I RCCGGY 2 cut(s) 208, 1019
Cfr13I GGNCC 9 cut(s) 131, 185, 284, 341, 395, 452, 1364, 1432, 2029
Cfr42I CCGCGG 2 cut(s) 867, 1221
CsiI ACCWGGT 1 cut(s) 2139
Csp6I GTAC 9 cut(s) 520, 637, 897, 1204, 1330, 1352, 1473, 1510, 1670
CspAI ACCGGT 1 cut(s) 208
CspCI CAANNNNNGTGG 4 cut(s) 987, 1022, 2007, 2042
CviAII CATG 9 cut(s) 74, 254, 437, 646, 788, 1125, 1313, 2059, 2093
CviQI GTAC 9 cut(s) 520, 637, 897, 1204, 1330, 1352, 1473, 1510, 1670
DdeI CTNAG 8 cut(s) 126, 294, 405, 850, 1277, 1640, 1673, 2081
DpnI GATC 5 cut(s) 1092, 1460, 1575, 1594, 1725
DpnII GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
DraI TTTAAA 1 cut(s) 1069
DraIII CACNNNGTG 1 cut(s) 1062
Eam1104I CTCTTC 1 cut(s) 1850
EarI CTCTTC 1 cut(s) 1850
Eco105I TACGTA 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 1157
Eco31I GGTCTC 1 cut(s) 1764
Eco47I GGWCC 7 cut(s) 131, 185, 284, 395, 452, 1432, 2029
Eco57I CTGAAG 5 cut(s) 151, 321, 432, 1181, 1701
Eco72I CACGTG 1 cut(s) 926
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 2 cut(s) 141, 351
Eco91I GGTNACC 1 cut(s) 68
EcoO109I RGGNCCY 4 cut(s) 131, 185, 452, 1432
EcoO65I GGTNACC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 1926
EcoRII CCWGG 4 cut(s) 1786, 1803, 2037, 2139
EcoT14I CCWWGG 1 cut(s) 645
EcoT22I ATGCAT 1 cut(s) 2064
EcoT38I GRGCYC 1 cut(s) 1157
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 9 cut(s) 77, 257, 440, 649, 791, 1128, 1316, 2062, 2096
FalI AAGNNNNNCTT 2 cut(s) 1238, 1270
FaqI GGGAC 8 cut(s) 28, 171, 260, 468, 1445, 1543, 1771, 2015
FatI CATG 9 cut(s) 73, 253, 436, 645, 787, 1124, 1312, 2058, 2092
FauI CCCGC 1 cut(s) 1568
FauNDI CATATG 2 cut(s) 1663, 1957
FokI GGATG 5 cut(s) 173, 232, 239, 383, 560
FriOI GRGCYC 1 cut(s) 1157
FspAI RTGCGCAY 1 cut(s) 1129
FspBI CTAG 1 cut(s) 1535
FspI TGCGCA 1 cut(s) 1129
GlaI GCGC 1 cut(s) 1129
HaeIII GGCC 3 cut(s) 233, 342, 1365
HapII CCGG 4 cut(s) 209, 941, 1020, 1629
HhaI GCGC 1 cut(s) 1130
Hin1II CATG 9 cut(s) 77, 257, 440, 649, 791, 1128, 1316, 2062, 2096
Hin6I GCGC 1 cut(s) 1128
HinP1I GCGC 1 cut(s) 1128
HindIII AAGCTT 1 cut(s) 1642
HinfI GANTC 9 cut(s) 146, 289, 346, 356, 400, 467, 481, 791, 1625
HpaII CCGG 4 cut(s) 209, 941, 1020, 1629
Hpy166II GTNNAC 1 cut(s) 1310
Hpy188I TCNGA 8 cut(s) 85, 288, 399, 1083, 1200, 1267, 1291, 2082
Hpy8I GTNNAC 1 cut(s) 1310
HpyCH4III ACNGT 4 cut(s) 366, 748, 1509, 1618
HpyCH4IV ACGT 4 cut(s) 639, 925, 1714, 1796
HpyF10VI GCNNNNNNNGC 5 cut(s) 331, 442, 731, 1053, 1422
HpyF3I CTNAG 8 cut(s) 126, 294, 405, 850, 1277, 1640, 1673, 2081
HpySE526I ACGT 4 cut(s) 639, 925, 1714, 1796
Hsp92II CATG 9 cut(s) 77, 257, 440, 649, 791, 1128, 1316, 2062, 2096
HspAI GCGC 1 cut(s) 1128
KflI GGGWCCC 2 cut(s) 185, 1432
Kpn2I TCCGGA 2 cut(s) 940, 1628
KspI CCGCGG 2 cut(s) 867, 1221
Kzo9I GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
LmnI GCTCC 2 cut(s) 866, 1403
Lsp1109I GCAGC 6 cut(s) 310, 421, 1035, 1312, 1502, 2029
LweI GCATC 3 cut(s) 1139, 1282, 1551
MabI ACCWGGT 1 cut(s) 2139
MaeI CTAG 1 cut(s) 1535
MaeII ACGT 4 cut(s) 639, 925, 1714, 1796
MaeIII GTNAC 6 cut(s) 68, 201, 500, 748, 769, 1852
MalI GATC 5 cut(s) 1092, 1460, 1575, 1594, 1725
MboI GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
MboII GAAGA 7 cut(s) 32, 157, 629, 1453, 1837, 1919, 2069
MfeI CAATTG 2 cut(s) 1183, 1454
MflI RGATCY 1 cut(s) 1723
MhlI GDGCHC 2 cut(s) 1157, 1312
MluI ACGCGT 1 cut(s) 323
MlyI GAGTC 5 cut(s) 155, 365, 476, 490, 1619
MmeI TCCRAC 3 cut(s) 273, 348, 2066
Mph1103I ATGCAT 1 cut(s) 2064
MroI TCCGGA 2 cut(s) 940, 1628
MroXI GAANNNNTTC 2 cut(s) 621, 1868
MslI CAYNNNNRTG 3 cut(s) 258, 1317, 2009
MspA1I CMGCKG 2 cut(s) 866, 1220
MspCI CTTAAG 3 cut(s) 112, 265, 376
MspI CCGG 4 cut(s) 209, 941, 1020, 1629
MspR9I CCNGG 4 cut(s) 1788, 1805, 2039, 2141
MunI CAATTG 2 cut(s) 1183, 1454
MvaI CCWGG 4 cut(s) 1788, 1805, 2039, 2141
MvnI CGCG 4 cut(s) 325, 866, 1220, 1238
MwoI GCNNNNNNNGC 5 cut(s) 331, 442, 731, 1053, 1422
NcoI CCATGG 1 cut(s) 645
NdeI CATATG 2 cut(s) 1663, 1957
NdeII GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
NlaIII CATG 9 cut(s) 77, 257, 440, 649, 791, 1128, 1316, 2062, 2096
NlaIV GGNNCC 8 cut(s) 132, 186, 187, 1216, 1399, 1433, 1434, 2031
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 4 cut(s) 68, 201, 748, 769
NruI TCGCGA 1 cut(s) 1238
NsbI TGCGCA 1 cut(s) 1129
NsiI ATGCAT 1 cut(s) 2064
NspI RCATGY 2 cut(s) 440, 2062
NspV TTCGAA 1 cut(s) 1848
PaeI GCATGC 1 cut(s) 2062
PaeR7I CTCGAG 2 cut(s) 141, 351
PceI AGGCCT 1 cut(s) 233
PdmI GAANNNNTTC 2 cut(s) 621, 1868
PfeI GAWTC 4 cut(s) 289, 346, 400, 791
Pfl23II CGTACG 1 cut(s) 1329
PinAI ACCGGT 1 cut(s) 208
PleI GAGTC 5 cut(s) 154, 364, 475, 489, 1619
PmaCI CACGTG 1 cut(s) 926
PmlI CACGTG 1 cut(s) 926
PpsI GAGTC 5 cut(s) 154, 364, 475, 489, 1619
Ppu21I YACGTR 2 cut(s) 640, 926
PpuMI RGGWCCY 4 cut(s) 131, 185, 452, 1432
PsiI TTATAA 1 cut(s) 1947
Psp1406I AACGTT 1 cut(s) 1796
Psp5II RGGWCCY 4 cut(s) 131, 185, 452, 1432
Psp6I CCWGG 4 cut(s) 1786, 1803, 2037, 2139
PspCI CACGTG 1 cut(s) 926
PspEI GGTNACC 1 cut(s) 68
PspGI CCWGG 4 cut(s) 1786, 1803, 2037, 2139
PspLI CGTACG 1 cut(s) 1329
PspN4I GGNNCC 8 cut(s) 132, 186, 187, 1216, 1399, 1433, 1434, 2031
PspPI GGNCC 9 cut(s) 131, 185, 284, 341, 395, 452, 1364, 1432, 2029
PspPPI RGGWCCY 4 cut(s) 131, 185, 452, 1432
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 1 cut(s) 1723
RruI TCGCGA 1 cut(s) 1238
RsaI GTAC 9 cut(s) 521, 638, 898, 1205, 1331, 1353, 1474, 1511, 1671
RsaNI GTAC 9 cut(s) 520, 637, 897, 1204, 1330, 1352, 1473, 1510, 1670
RseI CAYNNNNRTG 3 cut(s) 258, 1317, 2009
SacII CCGCGG 2 cut(s) 867, 1221
Sau3AI GATC 5 cut(s) 1090, 1458, 1573, 1592, 1723
Sau96I GGNCC 9 cut(s) 131, 185, 284, 341, 395, 452, 1364, 1432, 2029
ScaI AGTACT 2 cut(s) 898, 1353
SchI GAGTC 5 cut(s) 155, 365, 476, 490, 1619
ScrFI CCNGG 4 cut(s) 1788, 1805, 2039, 2141
SduI GDGCHC 2 cut(s) 1157, 1312
SexAI ACCWGGT 1 cut(s) 2139
SfaNI GCATC 3 cut(s) 1139, 1282, 1551
Sfr274I CTCGAG 2 cut(s) 141, 351
Sfr303I CCGCGG 2 cut(s) 867, 1221
SfuI TTCGAA 1 cut(s) 1848
SgrAI CRCCGGYG 1 cut(s) 208
SgrBI CCGCGG 2 cut(s) 867, 1221
SinI GGWCC 7 cut(s) 131, 185, 284, 395, 452, 1432, 2029
SlaI CTCGAG 2 cut(s) 141, 351
SmiMI CAYNNNNRTG 3 cut(s) 258, 1317, 2009
SmlI CTYRAG 5 cut(s) 112, 141, 265, 351, 376
SmoI CTYRAG 5 cut(s) 112, 141, 265, 351, 376
SnaBI TACGTA 1 cut(s) 640
SphI GCATGC 1 cut(s) 2062
SseBI AGGCCT 1 cut(s) 233
SspI AATATT 2 cut(s) 1707, 1918
SspMI CTAG 1 cut(s) 1535
StuI AGGCCT 1 cut(s) 233
StyD4I CCNGG 4 cut(s) 1786, 1803, 2037, 2139
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 4 cut(s) 366, 748, 1509, 1618
TaiI ACGT 4 cut(s) 642, 928, 1717, 1799
TaqI TCGA 7 cut(s) 142, 222, 352, 1151, 1257, 1623, 1848
TaqII GACCGA 3 cut(s) 1256, 1541, 1782
TatI WGTACW 5 cut(s) 519, 896, 1351, 1509, 1669
TauI GCSGC 6 cut(s) 334, 445, 869, 1223, 1226, 1970
TfiI GAWTC 4 cut(s) 289, 346, 400, 791
TscAI CASTG 5 cut(s) 315, 369, 858, 1681, 2031
TseFI GTSAC 4 cut(s) 68, 201, 748, 769
TseI GCWGC 6 cut(s) 298, 409, 1023, 1325, 1490, 2042
Tsp45I GTSAC 4 cut(s) 68, 201, 748, 769
TspDTI ATGAA 4 cut(s) 17, 234, 525, 1530
TspGWI ACGGA 1 cut(s) 1716
TspRI CASTG 5 cut(s) 315, 369, 858, 1681, 2031
Vha464I CTTAAG 3 cut(s) 112, 265, 376
VneI GTGCAC 1 cut(s) 1308
VpaK11BI GGWCC 7 cut(s) 131, 185, 284, 395, 452, 1432, 2029
XapI RAATTY 4 cut(s) 970, 1190, 1926, 2102
XceI RCATGY 2 cut(s) 440, 2062
XhoI CTCGAG 2 cut(s) 141, 351
XmnI GAANNNNTTC 2 cut(s) 621, 1868
XspI CTAG 1 cut(s) 1535
ZrmI AGTACT 2 cut(s) 898, 1353
Zsp2I ATGCAT 1 cut(s) 2064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.