Rh4CG106400

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0017135
Physical Location & Seq
Reverse (-)
19593232 .. 19594608
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG106400.1

Sequence Viewer

Length: 1377 bp
ATGGCAACCGCAGTCGCAGAACTTGTTAGGCTCTCTCTTATTACTTTCCTTTTAACCTCACACAGTTTATGCTTAACCGCCAACCCTGCCACAATCTCCATCGTCACAAAACCAAAGCGTTTTGTCACAAAACTCATTCACCATGATTCGGCTTTGTCTCCTTACTATAATCCCAATGCAAGCATTAGAGATATTGTTTCACGCTCAGTGAAAAGCTCTGCGGCACGCTTCACATACCTAAAAGCAAAGAGTACTAATGCTTTTGTGATGGACGATACACGTGCTGGTGTTGTTCCGGAGAAGTCTGCAGCAGAATTTATGGCAAATTTCTCGTTTGGAGAGCCACCAGTTCCACAGCTTTTGGCAATGGATACCGGCAGCAACTTATTGTGGATTCACTGCCTGCCTTGCCCCAAGTGTTTTAAACAATCCAATCCGATTTTTGATCCTTCCAAGTCCTCCACATACAAAACCTTGCCATGCGCATCTCCCGATTGTATTCAGTCGAGCCCTGGTGATAAGTGTGATTCCTCAAACAATTGCATATTCCTTCAGAAGTACCTTGATGGCACTGAGGCGGCAGGACTTATGGCGACTGAAAAGCTCACCTTGGAGACATCTGACGAAGGGCTTAGCCCCGTCTCCGATGCAGTTTTTGGCTGTGCACATGACAGTGTTGCTGACTACGATGGACAAATAAGTGGAGTACTTGGTCTTGGCCCTTCAGAAATATCTTTGACAACCAAGTTAGGCTCCAAGTTTTCTTATTGCATTGGAAGCATAAGGGACCCTAACTACCCTCACAACCAATTGATCTTCGGTGAGGGTACAAAGATAGAAGGGGCAGCAACCCCCCTTGAACTGTACAATGAACTTTACTATCTAACCCTGGAGGGCATCAGCCTCGGTCAGTCCCGCCTCCAAATTGATCCAAAAGTATTCACCAGATCACCATCAGGAACTGGTGGAACGGTTATCGACTCCGGAACTACACTAAGTTTCGTGGTTAAAAGTGCATATGATGTACTCAGTGCTGAAGTGCAAAAATTAGCAAACGGAATATTAGAACGTGTCCAAGATCCTGATACGCCCACAGCTCTTTGCTACAAGGGGACAATAGACCGAGACCTTGTTGGTTTTCCAGGTGCAACGTTTCACCTGGCAGGAGGGGTTGATTTGTTTCTGGACAAAACGAGCCTCTTCCAAGTTACTGGTGAGAATGAGTTCTGTTTAGCCGTGCAAATGCTAGAGGGCATGGGTCATGAAGATTTGAATGTTATTGGAATTCTGGCACAGCAAAATTATAATGTTGCATATGACATTGCCGCAGAGAAGGTTTATTTCCAGTACATTGACTGTGAGCTTCTTGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

458

Amino Acids

49.44

Weight (kDa)

4.9

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 106 - 274 4.1e-42 Xylanase inhibitor N-terminal
Asp PF00026 106 - 448 2.7e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 293 - 449 7.9e-29 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23945 AT4G30030 AT4G30040
fragaria_vesca FvH4_4g07480
malus_domestica MD16G1251100.v1.1 MD16G1251300.v1.1
prunus_persica Prupe.1G077600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399921
rosa_rugosa Rorug04G0020200
rosa_samantha Rh4BG094700 Rh4CG106400 Rh4DG091200
rosa_wichuraiana Rw4G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
Acc16I TGCGCA 1 cut(s) 484
AccIII TCCGGA 2 cut(s) 295, 983
AciI CCGC 6 cut(s) 9, 78, 221, 578, 916, 1326
AclI AACGTT 1 cut(s) 1151
AclWI GGATC 3 cut(s) 440, 923, 1073
AcsI RAATTY 3 cut(s) 314, 325, 1284
AcuI CTGAAG 3 cut(s) 536, 708, 1056
AcvI CACGTG 1 cut(s) 281
AfaI GTAC 7 cut(s) 253, 560, 708, 829, 866, 1026, 1349
AfiI CCNNNNNNNGG 1 cut(s) 148
AflIII ACRYGT 2 cut(s) 278, 1069
AgsI TTSAA 3 cut(s) 860, 1273, 1370
AjnI CCWGG 4 cut(s) 511, 888, 1141, 1158
AluBI AGCT 5 cut(s) 216, 358, 604, 1097, 1363
AluI AGCT 5 cut(s) 216, 358, 604, 1097, 1363
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 4 cut(s) 162, 608, 646, 1119
Alw44I GTGCAC 1 cut(s) 663
AlwI GGATC 3 cut(s) 440, 923, 1073
AlwNI CAGNNNCTG 1 cut(s) 962
Aor13HI TCCGGA 2 cut(s) 295, 983
AoxI GGCC 1 cut(s) 718
ApaLI GTGCAC 1 cut(s) 663
ApeKI GCWGC 3 cut(s) 308, 378, 845
ApoI RAATTY 3 cut(s) 314, 325, 1284
Asp700I GAANNNNTTC 1 cut(s) 1223
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 2 cut(s) 719, 787
AsuHPI GGTGA 8 cut(s) 131, 527, 598, 833, 934, 942, 1148, 1226
AvaII GGWCC 1 cut(s) 787
BaeGI GKGCMC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 512
BbrPI CACGTG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 3 cut(s) 320, 390, 857
BccI CCATC 5 cut(s) 107, 262, 560, 683, 961
BceAI ACGGC 1 cut(s) 1220
BcgI CGANNNNNNTGC 6 cut(s) 263, 297, 312, 346, 886, 920
BciT130I CCWGG 4 cut(s) 513, 890, 1143, 1160
BciVI GTATCC 1 cut(s) 364
BcoDI GTCTC 4 cut(s) 162, 608, 646, 1119
BfaI CTAG 1 cut(s) 1247
BfmI CTRYAG 1 cut(s) 306
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 6 cut(s) 222, 309, 379, 579, 846, 1326
BlpI GCTNAGC 1 cut(s) 632
BlsI GCNGC 6 cut(s) 223, 310, 380, 580, 847, 1327
BmcAI AGTACT 2 cut(s) 253, 708
Bme1390I CCNGG 4 cut(s) 513, 890, 1143, 1160
Bme18I GGWCC 1 cut(s) 787
BmgT120I GGNCC 2 cut(s) 719, 787
BmiI GGNNCC 3 cut(s) 754, 788, 789
BmrFI CCNGG 4 cut(s) 513, 890, 1143, 1160
BmsI GCATC 3 cut(s) 494, 637, 906
BpmI CTGGAG 1 cut(s) 911
Bpu1102I GCTNAGC 1 cut(s) 632
BsaAI YACGTR 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 952
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 4 cut(s) 511, 609, 888, 904
BsaWI WCCGGW 2 cut(s) 295, 983
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 4 cut(s) 347, 967, 1216, 1345
Bse3DI GCAATG 2 cut(s) 372, 1320
Bse8I GATNNNNATC 1 cut(s) 952
BseAI TCCGGA 2 cut(s) 295, 983
BseBI CCWGG 4 cut(s) 513, 890, 1143, 1160
BseDI CCNNGG 4 cut(s) 511, 609, 888, 904
BseJI GATNNNNATC 1 cut(s) 952
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMI GCAATG 2 cut(s) 372, 1320
BseMII CTCAG 3 cut(s) 219, 564, 1042
BseNI ACTGG 4 cut(s) 347, 967, 1216, 1345
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 3 cut(s) 320, 390, 857
BshFI GGCC 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 3 cut(s) 296, 375, 984
BslFI GGGAC 3 cut(s) 800, 898, 1126
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 4 cut(s) 162, 608, 646, 1119
BsmBI CGTCTC 1 cut(s) 646
BsmFI GGGAC 3 cut(s) 800, 898, 1126
BsnI GGCC 1 cut(s) 720
Bso31I GGTCTC 1 cut(s) 1119
Bsp1286I GDGCHC 2 cut(s) 512, 667
Bsp13I TCCGGA 2 cut(s) 295, 983
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 5 cut(s) 445, 813, 928, 947, 1078
Bsp1720I GCTNAGC 1 cut(s) 632
BspACI CCGC 6 cut(s) 9, 78, 221, 578, 916, 1326
BspANI GGCC 1 cut(s) 720
BspCNI CTCAG 3 cut(s) 218, 565, 1041
BspEI TCCGGA 2 cut(s) 295, 983
BspHI TCATGA 1 cut(s) 1261
BspLI GGNNCC 3 cut(s) 754, 788, 789
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 3 cut(s) 440, 923, 1073
BspTNI GGTCTC 1 cut(s) 1119
BsrDI GCAATG 2 cut(s) 372, 1320
BsrFI RCCGGY 1 cut(s) 374
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 4 cut(s) 347, 967, 1216, 1345
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 4 cut(s) 511, 609, 888, 904
BssMI GATC 5 cut(s) 445, 813, 928, 947, 1078
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 4 cut(s) 513, 890, 1143, 1160
Bst4CI ACNGT 5 cut(s) 65, 674, 864, 973, 1358
Bst6I CTCTTC 1 cut(s) 1205
BstAUI TGTACA 1 cut(s) 864
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 181, 226, 404
BstDEI CTNAG 5 cut(s) 205, 573, 632, 995, 1028
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 5 cut(s) 448, 816, 931, 950, 1081
BstMAI GTCTC 4 cut(s) 162, 608, 646, 1119
BstMBI GATC 5 cut(s) 445, 813, 928, 947, 1078
BstMWI GCNNNNNNNGC 3 cut(s) 86, 408, 777
BstNI CCWGG 4 cut(s) 513, 890, 1143, 1160
BstSCI CCNGG 4 cut(s) 511, 888, 1141, 1158
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 3 cut(s) 320, 390, 857
BstX2I RGATCY 1 cut(s) 1078
BstXI CCANNNNNNTGG 1 cut(s) 1211
BstYI RGATCY 1 cut(s) 1078
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 720
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 5 cut(s) 213, 397, 570, 679, 1036
Cac8I GCNNGC 3 cut(s) 181, 226, 404
CaiI CAGNNNCTG 1 cut(s) 962
CciI TCATGA 1 cut(s) 1261
CfoI GCGC 1 cut(s) 485
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 719, 787
Csp6I GTAC 7 cut(s) 252, 559, 707, 828, 865, 1025, 1348
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 5 cut(s) 143, 480, 668, 1255, 1262
CviQI GTAC 7 cut(s) 252, 559, 707, 828, 865, 1025, 1348
DdeI CTNAG 5 cut(s) 205, 573, 632, 995, 1028
DpnI GATC 5 cut(s) 447, 815, 930, 949, 1080
DpnII GATC 5 cut(s) 445, 813, 928, 947, 1078
DraI TTTAAA 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 1205
EarI CTCTTC 1 cut(s) 1205
Eco130I CCWWGG 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 512
Eco31I GGTCTC 1 cut(s) 1119
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 3 cut(s) 536, 708, 1056
Eco72I CACGTG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 787
EcoRI GAATTC 1 cut(s) 1284
EcoRII CCWGG 4 cut(s) 511, 888, 1141, 1158
EcoT14I CCWWGG 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 646
FaeI CATG 5 cut(s) 146, 483, 671, 1258, 1265
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 3 cut(s) 800, 898, 1126
FatI CATG 5 cut(s) 142, 479, 667, 1254, 1261
FauI CCCGC 1 cut(s) 923
FauNDI CATATG 2 cut(s) 1018, 1315
Fnu4HI GCNGC 6 cut(s) 222, 309, 379, 579, 846, 1326
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 6 cut(s) 222, 309, 379, 579, 846, 1326
FspAI RTGCGCAY 1 cut(s) 484
FspBI CTAG 1 cut(s) 1247
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 1 cut(s) 484
GluI GCNGC 6 cut(s) 222, 309, 379, 579, 846, 1326
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 1 cut(s) 720
HapII CCGG 3 cut(s) 296, 375, 984
HhaI GCGC 1 cut(s) 485
Hin1II CATG 5 cut(s) 146, 483, 671, 1258, 1265
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HinfI GANTC 4 cut(s) 146, 394, 527, 980
HpaII CCGG 3 cut(s) 296, 375, 984
HphI GGTGA 8 cut(s) 131, 527, 598, 833, 934, 942, 1148, 1226
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 5 cut(s) 438, 555, 622, 646, 727
Hpy188III TCNNGA 8 cut(s) 296, 491, 957, 984, 1082, 1184, 1262, 1367
Hpy8I GTNNAC 1 cut(s) 665
HpyAV CCTTC 6 cut(s) 459, 560, 620, 732, 833, 1327
HpyCH4III ACNGT 5 cut(s) 65, 674, 864, 973, 1358
HpyCH4IV ACGT 3 cut(s) 280, 1069, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 408, 777
HpyF3I CTNAG 5 cut(s) 205, 573, 632, 995, 1028
HpySE526I ACGT 3 cut(s) 280, 1069, 1151
Hsp92II CATG 5 cut(s) 146, 483, 671, 1258, 1265
HspAI GCGC 1 cut(s) 483
KflI GGGWCCC 1 cut(s) 787
Kpn2I TCCGGA 2 cut(s) 295, 983
Kzo9I GATC 5 cut(s) 445, 813, 928, 947, 1078
LmnI GCTCC 1 cut(s) 758
Lsp1109I GCAGC 3 cut(s) 320, 390, 857
LweI GCATC 3 cut(s) 494, 637, 906
MaeI CTAG 1 cut(s) 1247
MaeII ACGT 3 cut(s) 280, 1069, 1151
MaeIII GTNAC 3 cut(s) 103, 124, 1207
MalI GATC 5 cut(s) 447, 815, 930, 949, 1080
MboI GATC 5 cut(s) 445, 813, 928, 947, 1078
MboII GAAGA 3 cut(s) 808, 1192, 1277
MfeI CAATTG 2 cut(s) 538, 809
MflI RGATCY 1 cut(s) 1078
MhlI GDGCHC 2 cut(s) 512, 667
MluCI AATT 8 cut(s) 314, 325, 538, 809, 924, 1046, 1284, 1300
MlyI GAGTC 1 cut(s) 974
MroI TCCGGA 2 cut(s) 295, 983
MroXI GAANNNNTTC 1 cut(s) 1223
MseI TTAA 4 cut(s) 53, 74, 423, 1008
MslI CAYNNNNRTG 1 cut(s) 672
MspI CCGG 3 cut(s) 296, 375, 984
MspR9I CCNGG 4 cut(s) 513, 890, 1143, 1160
MunI CAATTG 2 cut(s) 538, 809
MvaI CCWGG 4 cut(s) 513, 890, 1143, 1160
MwoI GCNNNNNNNGC 3 cut(s) 86, 408, 777
NdeI CATATG 2 cut(s) 1018, 1315
NdeII GATC 5 cut(s) 445, 813, 928, 947, 1078
NlaIII CATG 5 cut(s) 146, 483, 671, 1258, 1265
NlaIV GGNNCC 3 cut(s) 754, 788, 789
NmuCI GTSAC 2 cut(s) 103, 124
NsbI TGCGCA 1 cut(s) 484
PagI TCATGA 1 cut(s) 1261
PdmI GAANNNNTTC 1 cut(s) 1223
PfeI GAWTC 3 cut(s) 146, 394, 527
PkrI GCNGC 6 cut(s) 223, 310, 380, 580, 847, 1327
PleI GAGTC 1 cut(s) 974
PmaCI CACGTG 1 cut(s) 281
PmlI CACGTG 1 cut(s) 281
PpsI GAGTC 1 cut(s) 974
Ppu21I YACGTR 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 787
PsiI TTATAA 1 cut(s) 1305
Psp1406I AACGTT 1 cut(s) 1151
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 4 cut(s) 511, 888, 1141, 1158
PspCI CACGTG 1 cut(s) 281
PspGI CCWGG 4 cut(s) 511, 888, 1141, 1158
PspN4I GGNNCC 3 cut(s) 754, 788, 789
PspPI GGNCC 2 cut(s) 719, 787
PspPPI RGGWCCY 1 cut(s) 787
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 962
PsuI RGATCY 1 cut(s) 1078
RsaI GTAC 7 cut(s) 253, 560, 708, 829, 866, 1026, 1349
RsaNI GTAC 7 cut(s) 252, 559, 707, 828, 865, 1025, 1348
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 4 cut(s) 53, 74, 423, 1008
SatI GCNGC 6 cut(s) 222, 309, 379, 579, 846, 1326
Sau3AI GATC 5 cut(s) 445, 813, 928, 947, 1078
Sau96I GGNCC 2 cut(s) 719, 787
ScaI AGTACT 2 cut(s) 253, 708
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 4 cut(s) 513, 890, 1143, 1160
SduI GDGCHC 2 cut(s) 512, 667
SfaNI GCATC 3 cut(s) 494, 637, 906
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 672
Sse9I AATT 8 cut(s) 314, 325, 538, 809, 924, 1046, 1284, 1300
SsiI CCGC 6 cut(s) 9, 78, 221, 578, 916, 1326
SspI AATATT 1 cut(s) 1062
SspMI CTAG 1 cut(s) 1247
StyD4I CCNGG 4 cut(s) 511, 888, 1141, 1158
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 5 cut(s) 65, 674, 864, 973, 1358
TaiI ACGT 3 cut(s) 283, 1072, 1154
TaqI TCGA 2 cut(s) 506, 978
TaqII GACCGA 2 cut(s) 896, 1137
TasI AATT 8 cut(s) 314, 325, 538, 809, 924, 1046, 1284, 1300
TatI WGTACW 5 cut(s) 251, 706, 864, 1024, 1347
TauI GCSGC 3 cut(s) 224, 581, 1328
TfiI GAWTC 3 cut(s) 146, 394, 527
Tru1I TTAA 4 cut(s) 53, 74, 423, 1008
Tru9I TTAA 4 cut(s) 53, 74, 423, 1008
TscAI CASTG 5 cut(s) 213, 404, 577, 679, 1036
TseFI GTSAC 2 cut(s) 103, 124
TseI GCWGC 3 cut(s) 308, 378, 845
Tsp45I GTSAC 2 cut(s) 103, 124
TspDTI ATGAA 2 cut(s) 885, 1278
TspGWI ACGGA 1 cut(s) 1071
TspRI CASTG 5 cut(s) 213, 404, 577, 679, 1036
VneI GTGCAC 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 3 cut(s) 314, 325, 1284
XmnI GAANNNNTTC 1 cut(s) 1223
XspI CTAG 1 cut(s) 1247
ZrmI AGTACT 2 cut(s) 253, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.