AT4G30040

Xylanase inhibitor N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017135
Physical Location & Seq
Forward (+)
14685602 .. 14686885
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30040.1

Sequence Viewer

Length: 1284 bp
ATGGCGATTTTCTTTACATCTCCCCTCTTCTTTCTCATCATCCTTTGTTTCTCTATCTCCGTGGTTCACTTGTCAGCATCACCAACTCTTGTTCTCAATCTCGTTCATTCCTACCACATATACTCTCGAAAACCACCACATGTCTATCATATCAAGGAAGCGTCGGTCGAACGTCTAGAGTACTTGAAAGCCAAGACAACTGGAGATATCATTGCACACCTTTCCCCTAACGTCCCTATCATCCCACAAGCTTTCTTAGTGAACATATCAATAGGTAGCCCACCAATCACACAACTTCTCCACATGGACACGGCTAGTGATCTTCTATGGATACAATGTCTTCCTTGTATCAATTGCTATGCTCAAAGTCTTCCTATTTTCGATCCTTCTCGATCTTATACTCACCGCAACGAAACATGTAGAACATCTCAATACTCTATGCCTAGCTTAAAATTCAATGCAAACACAAGATCTTGCGAGTATTCCATGAGATACGTGGATGATACAGGGAGCAAAGGCATACTAGCTAGAGAAATGTTGCTGTTTAATACAATCTACGATGAGTCTTCGTCAGCTGCTTTACATGACGTTGTTTTTGGTTGTGGCCATGATAACTACGGTGAGCCTCTAGTTGGAACCGGCATTCTCGGTTTAGGGTATGGTGAATTCTCGTTGGTTCATAGATTTGGTAAAAAGTTCTCATACTGCTTTGGAAGCTTAGATGATCCTTCTTATCCACATAACGTCTTAGTCTTGGGAGATGATGGTGCCAACATTTTGGGAGATACCACACCGTTAGAGATCCACAATGGTTTTTATTACGTTACAATCGAAGCCATAAGCGTGGATGGAATCATTCTCCCGATCGATCCACGTGTATTCAACCGTAATCACCAAACCGGTTTAGGCGGTACAATAATCGACACGGGAAATTCATTAACAAGCCTAGTCGAAGAAGCTTACAAGCCATTGAAGAACAGAATTGAAGATATTTTTGAAGGAAGGTTTACGGCAGCGGATGTGAGTCAAGATGACATGATTAAAATGGAGTGCTACAATGGAAACTTTGAGCGAGATTTGGTCGAGTCTGGATTTCCGATTGTAACGTTTCATTTCTCAGAAGGAGCAGAGTTGTCATTGGATGTGAAGAGTTTGTTCATGAAGCTTTCACCAAATGTTTTCTGCTTAGCCGTGACTCCTGGTAATTTGAACAGCATTGGTGCGACGGCTCAACAGAGTTATAACATAGGCTATGATCTCGAAGCAATGGAGGTTTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

427

Amino Acids

47.36

Weight (kDa)

5.12

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 85 - 254 2.3e-38 Xylanase inhibitor N-terminal
Asp PF00026 85 - 427 4.1e-07 Eukaryotic aspartyl protease
TAXi_C PF14541 273 - 427 3.2e-26 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23945 AT4G30030 AT4G30040
fragaria_vesca FvH4_4g07480
malus_domestica MD16G1251100.v1.1 MD16G1251300.v1.1
prunus_persica Prupe.1G077600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399921
rosa_rugosa Rorug04G0020200
rosa_samantha Rh4BG094700 Rh4CG106400 Rh4DG091200
rosa_wichuraiana Rw4G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1242
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 3 cut(s) 406, 909, 1016
AclI AACGTT 1 cut(s) 1106
AclWI GGATC 4 cut(s) 377, 719, 796, 863
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 3 cut(s) 452, 665, 931
AcvI CACGTG 1 cut(s) 875
AfaI GTAC 2 cut(s) 182, 913
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 3 cut(s) 139, 416, 874
AgeI ACCGGT 1 cut(s) 899
AgsI TTSAA 7 cut(s) 187, 457, 883, 973, 986, 998, 1210
AjnI CCWGG 1 cut(s) 1198
AluBI AGCT 7 cut(s) 251, 447, 527, 575, 717, 959, 1165
AluI AGCT 7 cut(s) 251, 447, 527, 575, 717, 959, 1165
AlwI GGATC 4 cut(s) 377, 719, 796, 863
AoxI GGCC 1 cut(s) 604
ApeKI GCWGC 2 cut(s) 575, 1013
ApoI RAATTY 3 cut(s) 452, 665, 931
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AsiGI ACCGGT 1 cut(s) 899
AsuHPI GGTGA 6 cut(s) 72, 395, 632, 674, 884, 1161
BalI TGGCCA 1 cut(s) 606
BanI GGYRCC 1 cut(s) 767
BbrPI CACGTG 1 cut(s) 875
BbsI GAAGAC 3 cut(s) 332, 362, 558
BbvI GCAGC 2 cut(s) 562, 1025
BccI CCATC 2 cut(s) 758, 842
BceAI ACGGC 4 cut(s) 327, 1026, 1175, 1242
BciT130I CCWGG 1 cut(s) 1200
BciVI GTATCC 1 cut(s) 324
BfaI CTAG 7 cut(s) 176, 315, 444, 524, 528, 629, 947
BfuI GTATCC 1 cut(s) 324
BglII AGATCT 1 cut(s) 470
BisI GCNGC 2 cut(s) 576, 1014
BlpI GCTNAGC 1 cut(s) 1186
BlsI GCNGC 2 cut(s) 577, 1015
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 1200
BmiI GGNNCC 2 cut(s) 637, 769
BmrFI CCNGG 1 cut(s) 1200
BmsI GCATC 1 cut(s) 86
BpiI GAAGAC 3 cut(s) 332, 362, 558
BpmI CTGGAG 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 1186
Bsa29I ATCGAT 1 cut(s) 867
BsaAI YACGTR 2 cut(s) 496, 875
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 899
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse118I RCCGGY 2 cut(s) 638, 899
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 2 cut(s) 210, 1272
BseBI CCWGG 1 cut(s) 1200
BseCI ATCGAT 1 cut(s) 867
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 6 cut(s) 39, 240, 505, 853, 1024, 1147
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMI GCAATG 2 cut(s) 210, 1272
BseMII CTCAG 1 cut(s) 1131
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 562, 1025
Bsh1285I CGRYCG 2 cut(s) 168, 867
BshFI GGCC 1 cut(s) 606
BshNI GGYRCC 1 cut(s) 767
BshTI ACCGGT 1 cut(s) 899
BshVI ATCGAT 1 cut(s) 867
BsiEI CGRYCG 2 cut(s) 168, 867
BsiSI CCGG 2 cut(s) 639, 900
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 632
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 1 cut(s) 606
Bsp143I GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
Bsp1720I GCTNAGC 1 cut(s) 1186
BspACI CCGC 3 cut(s) 406, 909, 1016
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 1 cut(s) 1130
BspDI ATCGAT 1 cut(s) 867
BspHI TCATGA 1 cut(s) 1158
BspLI GGNNCC 2 cut(s) 637, 769
BspPI GGATC 4 cut(s) 377, 719, 796, 863
BspT107I GGYRCC 1 cut(s) 767
BsrDI GCAATG 2 cut(s) 210, 1272
BsrFI RCCGGY 2 cut(s) 638, 899
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 2 cut(s) 638, 899
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
Bst2UI CCWGG 1 cut(s) 1200
Bst4CI ACNGT 3 cut(s) 620, 795, 887
Bst6I CTCTTC 2 cut(s) 32, 1142
BstBAI YACGTR 2 cut(s) 496, 875
BstDEI CTNAG 5 cut(s) 256, 718, 748, 1117, 1186
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 6 cut(s) 39, 240, 505, 853, 1024, 1147
BstKTI GATC 9 cut(s) 322, 385, 395, 473, 727, 804, 867, 871, 1258
BstMBI GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
BstMCI CGRYCG 2 cut(s) 168, 867
BstMWI GCNNNNNNNGC 1 cut(s) 714
BstNI CCWGG 1 cut(s) 1200
BstNSI RCATGY 2 cut(s) 143, 420
BstSCI CCNGG 1 cut(s) 1198
BstV1I GCAGC 2 cut(s) 562, 1025
BstV2I GAAGAC 3 cut(s) 332, 362, 558
BstX2I RGATCY 2 cut(s) 470, 801
BstXI CCANNNNNNTGG 2 cut(s) 778, 844
BstYI RGATCY 2 cut(s) 470, 801
Bsu15I ATCGAT 1 cut(s) 867
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 1 cut(s) 606
BsuTUI ATCGAT 1 cut(s) 867
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 6 cut(s) 39, 240, 505, 853, 1024, 1147
CciI TCATGA 1 cut(s) 1158
Cfr10I RCCGGY 2 cut(s) 638, 899
ClaI ATCGAT 1 cut(s) 867
CseI GACGC 1 cut(s) 150
Csp6I GTAC 2 cut(s) 181, 912
CspAI ACCGGT 1 cut(s) 899
CviAII CATG 8 cut(s) 140, 304, 417, 487, 584, 608, 1036, 1159
CviQI GTAC 2 cut(s) 181, 912
DdeI CTNAG 5 cut(s) 256, 718, 748, 1117, 1186
DpnI GATC 9 cut(s) 321, 384, 394, 472, 726, 803, 866, 870, 1257
DpnII GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 32, 1142
EarI CTCTTC 2 cut(s) 32, 1142
Eco32I GATATC 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 1198
EcoRV GATATC 1 cut(s) 208
FaeI CATG 8 cut(s) 143, 307, 420, 490, 587, 611, 1039, 1162
FaqI GGGAC 1 cut(s) 218
FatI CATG 8 cut(s) 139, 303, 416, 486, 583, 607, 1035, 1158
Fnu4HI GCNGC 2 cut(s) 576, 1014
FokI GGATG 6 cut(s) 26, 227, 512, 860, 1031, 1154
Fsp4HI GCNGC 2 cut(s) 576, 1014
FspBI CTAG 7 cut(s) 176, 315, 444, 524, 528, 629, 947
GluI GCNGC 2 cut(s) 576, 1014
GsuI CTGGAG 1 cut(s) 222
HaeIII GGCC 1 cut(s) 606
HapII CCGG 2 cut(s) 639, 900
HgaI GACGC 1 cut(s) 150
Hin1II CATG 8 cut(s) 143, 307, 420, 490, 587, 611, 1039, 1162
HindIII AAGCTT 4 cut(s) 249, 715, 957, 1163
HinfI GANTC 5 cut(s) 563, 852, 1024, 1085, 1195
HpaII CCGG 2 cut(s) 639, 900
HphI GGTGA 6 cut(s) 72, 395, 632, 674, 884, 1161
Hpy166II GTNNAC 3 cut(s) 67, 262, 1008
Hpy188I TCNGA 2 cut(s) 1098, 1120
Hpy188III TCNNGA 8 cut(s) 126, 176, 390, 862, 1028, 1089, 1159, 1259
Hpy8I GTNNAC 3 cut(s) 67, 262, 1008
Hpy99I CGWCG 2 cut(s) 166, 1228
HpyAV CCTTC 5 cut(s) 396, 738, 992, 996, 1115
HpyCH4III ACNGT 3 cut(s) 620, 795, 887
HpyCH4IV ACGT 8 cut(s) 172, 231, 495, 588, 744, 822, 874, 1106
HpyCH4V TGCA 2 cut(s) 215, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 714
HpyF3I CTNAG 5 cut(s) 256, 718, 748, 1117, 1186
HpySE526I ACGT 8 cut(s) 172, 231, 495, 588, 744, 822, 874, 1106
Hsp92II CATG 8 cut(s) 143, 307, 420, 490, 587, 611, 1039, 1162
Kzo9I GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
LmnI GCTCC 2 cut(s) 510, 1124
LpnPI CCDG 7 cut(s) 186, 492, 652, 913, 1074, 1185, 1212
Lsp1109I GCAGC 2 cut(s) 562, 1025
LweI GCATC 1 cut(s) 86
MaeI CTAG 7 cut(s) 176, 315, 444, 524, 528, 629, 947
MaeII ACGT 8 cut(s) 172, 231, 495, 588, 744, 822, 874, 1106
MaeIII GTNAC 3 cut(s) 823, 1102, 1192
MalI GATC 9 cut(s) 321, 384, 394, 472, 726, 803, 866, 870, 1257
MboI GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
MboII GAAGA 9 cut(s) 19, 314, 332, 362, 558, 965, 985, 998, 1159
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 2 cut(s) 470, 801
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 6 cut(s) 352, 452, 665, 931, 981, 1204
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 4 cut(s) 572, 1033, 1094, 1189
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 3 cut(s) 35, 636, 1264
Mox20I TGGCCA 1 cut(s) 606
MscI TGGCCA 1 cut(s) 606
MseI TTAA 4 cut(s) 449, 546, 938, 1041
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 2 cut(s) 575, 1016
MspI CCGG 2 cut(s) 639, 900
MspR9I CCNGG 1 cut(s) 1200
MunI CAATTG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 1 cut(s) 1200
MwoI GCNNNNNNNGC 1 cut(s) 714
NdeII GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
NlaIII CATG 8 cut(s) 143, 307, 420, 490, 587, 611, 1039, 1162
NlaIV GGNNCC 2 cut(s) 637, 769
NmuCI GTSAC 1 cut(s) 1192
NspI RCATGY 2 cut(s) 143, 420
PagI TCATGA 1 cut(s) 1158
PciI ACATGT 2 cut(s) 139, 416
PcsI WCGNNNNNNNCGW 1 cut(s) 828
PctI GAATGC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 852
PinAI ACCGGT 1 cut(s) 899
PkrI GCNGC 2 cut(s) 577, 1015
Ple19I CGATCG 1 cut(s) 867
PleI GAGTC 4 cut(s) 571, 1032, 1093, 1189
PmaCI CACGTG 1 cut(s) 875
PmlI CACGTG 1 cut(s) 875
PpsI GAGTC 4 cut(s) 571, 1032, 1093, 1189
Ppu21I YACGTR 2 cut(s) 496, 875
PscI ACATGT 2 cut(s) 139, 416
PsiI TTATAA 1 cut(s) 1242
Psp1406I AACGTT 1 cut(s) 1106
Psp6I CCWGG 1 cut(s) 1198
PspCI CACGTG 1 cut(s) 875
PspGI CCWGG 1 cut(s) 1198
PspN4I GGNNCC 2 cut(s) 637, 769
PsuI RGATCY 2 cut(s) 470, 801
PvuI CGATCG 1 cut(s) 867
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 182, 913
RsaNI GTAC 2 cut(s) 181, 912
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 4 cut(s) 449, 546, 938, 1041
SatI GCNGC 2 cut(s) 576, 1014
Sau3AI GATC 9 cut(s) 319, 382, 392, 470, 724, 801, 864, 868, 1255
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 4 cut(s) 572, 1033, 1094, 1189
ScrFI CCNGG 1 cut(s) 1200
SfaNI GCATC 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 842
Sse9I AATT 6 cut(s) 352, 452, 665, 931, 981, 1204
SsiI CCGC 3 cut(s) 406, 909, 1016
SspMI CTAG 7 cut(s) 176, 315, 444, 524, 528, 629, 947
StyD4I CCNGG 1 cut(s) 1198
TaaI ACNGT 3 cut(s) 620, 795, 887
TaiI ACGT 8 cut(s) 175, 234, 498, 591, 747, 825, 877, 1109
TaqII GACCGA 1 cut(s) 154
TasI AATT 6 cut(s) 352, 452, 665, 931, 981, 1204
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 852
Tru1I TTAA 4 cut(s) 449, 546, 938, 1041
Tru9I TTAA 4 cut(s) 449, 546, 938, 1041
TseFI GTSAC 1 cut(s) 1192
TseI GCWGC 2 cut(s) 575, 1013
Tsp45I GTSAC 1 cut(s) 1192
TspDTI ATGAA 6 cut(s) 95, 668, 924, 1100, 1147, 1175
TspGWI ACGGA 1 cut(s) 49
XapI RAATTY 3 cut(s) 452, 665, 931
XbaI TCTAGA 1 cut(s) 175
XceI RCATGY 2 cut(s) 143, 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XspI CTAG 7 cut(s) 176, 315, 444, 524, 528, 629, 947
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.