AT4G30420

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016648
Physical Location & Seq
Forward (+)
14876905 .. 14878914
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30420.2

Sequence Viewer

Length: 1122 bp
ATGGCGATGACGATGATTCAACTATGTTATGCAGGAGTGACTCTCTTTGCAAGAGCTACTTTGGTTCATGGATTAAGCCCTCGGGTTTTTATCCTCTATAGGCAAGCCTTTGCCACCATTTTCATCTTCCCATTTCTTTACTTATCCAGGAGAAAATCAAAGATAGCTATATCTTCTTTGGATCTAAAGAGCTTTTCTTTAATATTCTTGGTCTCGCTTATAGGCATTACAATCAATCAAAATCTATATCTTGAAGGTCTTTACTTAACTTCATCATCAATGGGAAGTGCAGTGGGTAACATCATCCCTGCAATCACCTTCCTCATCTCATTTCTCGCCGGATACGAGAAACTGAATCTCCGAGATATAAGAGGCTTAGCAAAGATCGCAGGGACGATCCTATGTGTAGCAGGAGCGATCTCCATGACTCTTCTTCGTGGACCAAAGATTCTCAACTCAGAATCCGCTTTACCGATCGCTAAATCGGTATTAGGACATCTTAAAGACCAGAACACGTGGTTGATTGGCTGTTTGTTCTTGTTTTCTAGCACTCTTTGTTGGTCCTTTTGGCTTATACTTCAGGTTCCAATCTCTGCCTACTATCCAGATAACCTCTCTTTATCAGCATGGATGTGTTTATTCGGAACAATACAATGTGCAGTCGTCACTTTCTTCCTTGAGAAAGACCCAAACGCTTGGATTCTCCATTCCTACTCCGAGTTTGCCACATGTCTCTATGCAGGAATTGGGGCGTCAGCACTTTCTTTTACGGTCCAAGCTTGGGCTATTGCAAAGAGAGGTCCTGTGTTTTCTGCACTGTTTAATCCTCTTTGTACAGTCATTGTCACAATCTTGGCTGCTTTGTTCTTCCATGAAGAGATTTACACTGGAAGTTTAATCGGAGGATTGGGCGTGATATTGGGACTTTACACTGTGCTCTGGGGTAAAGCAAAAGATGTCATGATGAATCAAGATCAGAGAGACAATGATCAGAAGTCAGAAGTGAAGATTCATATAGAAGATTCTTCAAACACAACAATCTGTAATAAAGATCTGAAGAATCCTCTGTTGTCCAAACACAAATCAACAGAAGAAATCCAAACACATCAACAACTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

373

Amino Acids

41.28

Weight (kDa)

8.77

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 16 - 146 3.7e-12 EamA-like transporter family
EamA PF00892 185 - 323 4.6e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 465
AclWI GGATC 2 cut(s) 189, 391
AcuI CTGAAG 2 cut(s) 563, 1076
AcvI CACGTG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 752
AfaI GTAC 1 cut(s) 835
AfiI CCNNNNNNNGG 1 cut(s) 781
AflIII ACRYGT 2 cut(s) 513, 728
AgsI TTSAA 3 cut(s) 20, 254, 1029
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 4 cut(s) 56, 167, 192, 779
AluI AGCT 4 cut(s) 56, 167, 192, 779
Alw21I GWGCWC 1 cut(s) 939
Alw26I GTCTC 3 cut(s) 217, 737, 975
AlwI GGATC 2 cut(s) 189, 391
Ama87I CYCGRG 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 857
AspS9I GGNCC 4 cut(s) 440, 561, 772, 800
AsuHPI GGTGA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 4 cut(s) 440, 561, 772, 800
BbrPI CACGTG 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 939
BbvI GCAGC 1 cut(s) 844
BciT130I CCWGG 1 cut(s) 148
BciVI GTATCC 1 cut(s) 335
BclI TGATCA 1 cut(s) 988
BcoDI GTCTC 3 cut(s) 217, 737, 975
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 1 cut(s) 97
BfuI GTATCC 1 cut(s) 335
BglII AGATCT 1 cut(s) 1051
BisI GCNGC 1 cut(s) 858
BlpI GCTNAGC 1 cut(s) 376
BlsI GCNGC 1 cut(s) 859
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 4 cut(s) 440, 561, 772, 800
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 4 cut(s) 440, 561, 772, 800
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 1 cut(s) 148
BplI GAGNNNNNCTC 2 cut(s) 27, 59
Bpu1102I GCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse1I ACTGG 1 cut(s) 892
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 2 cut(s) 303, 636
BseLI CCNNNNNNNGG 1 cut(s) 781
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 1 cut(s) 892
BseXI GCAGC 1 cut(s) 844
BsgI GTGCAG 3 cut(s) 309, 678, 798
Bsh1285I CGRYCG 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 939
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 2 cut(s) 406, 936
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 3 cut(s) 217, 737, 975
BsmFI GGGAC 2 cut(s) 406, 936
Bso31I GGTCTC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 939
Bsp1407I TGTACA 1 cut(s) 833
Bsp143I GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
Bsp1720I GCTNAGC 1 cut(s) 376
BspACI CCGC 1 cut(s) 465
BspCNI CTCAG 1 cut(s) 470
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 1 cut(s) 585
BspPI GGATC 2 cut(s) 189, 391
BspTNI GGTCTC 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 833
BsrI ACTGG 1 cut(s) 892
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
BssNI GRCGYC 1 cut(s) 752
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 4 cut(s) 772, 819, 838, 934
Bst6I CTCTTC 2 cut(s) 435, 870
BstACI GRCGYC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 376, 457
BstF5I GGATG 2 cut(s) 303, 636
BstKTI GATC 8 cut(s) 184, 387, 399, 420, 477, 976, 991, 1054
BstMAI GTCTC 3 cut(s) 217, 737, 975
BstMBI GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 146
BstSFI CTRYAG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 844
BstX2I RGATCY 2 cut(s) 181, 1051
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 2 cut(s) 181, 1051
BsuI GTATCC 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 303, 636
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 4 cut(s) 297, 815, 885, 930
Cac8I GCNNGC 1 cut(s) 105
CciI TCATGA 1 cut(s) 960
Cfr13I GGNCC 4 cut(s) 440, 561, 772, 800
CseI GACGC 1 cut(s) 741
Csp6I GTAC 1 cut(s) 834
CviAII CATG 6 cut(s) 68, 424, 627, 729, 872, 961
CviQI GTAC 1 cut(s) 834
DdeI CTNAG 2 cut(s) 376, 457
DpnI GATC 8 cut(s) 183, 386, 398, 419, 476, 975, 990, 1053
DpnII GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
Eam1104I CTCTTC 2 cut(s) 435, 870
EarI CTCTTC 2 cut(s) 435, 870
Eco31I GGTCTC 1 cut(s) 217
Eco47I GGWCC 4 cut(s) 440, 561, 772, 800
Eco57I CTGAAG 2 cut(s) 563, 1076
Eco72I CACGTG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 71, 427, 630, 732, 875, 964
FalI AAGNNNNNCTT 2 cut(s) 43, 75
FaqI GGGAC 2 cut(s) 406, 936
FatI CATG 6 cut(s) 67, 423, 626, 728, 871, 960
FbaI TGATCA 1 cut(s) 988
Fnu4HI GCNGC 1 cut(s) 858
FokI GGATG 2 cut(s) 290, 643
Fsp4HI GCNGC 1 cut(s) 858
FspBI CTAG 1 cut(s) 546
GluI GCNGC 1 cut(s) 858
HapII CCGG 1 cut(s) 339
HgaI GACGC 1 cut(s) 741
Hin1I GRCGYC 1 cut(s) 752
Hin1II CATG 6 cut(s) 71, 427, 630, 732, 875, 964
HindIII AAGCTT 1 cut(s) 777
HpaII CCGG 1 cut(s) 339
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 9 cut(s) 362, 460, 644, 718, 902, 978, 993, 1000, 1056
Hpy188III TCNNGA 4 cut(s) 251, 605, 961, 971
Hpy8I GTNNAC 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 248, 328
HpyCH4III ACNGT 4 cut(s) 772, 819, 838, 934
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 8 cut(s) 32, 50, 290, 311, 659, 740, 791, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 2 cut(s) 376, 457
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 1 cut(s) 752
Hsp92II CATG 6 cut(s) 71, 427, 630, 732, 875, 964
Ksp22I TGATCA 1 cut(s) 988
Kzo9I GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
LmnI GCTCC 1 cut(s) 413
Lsp1109I GCAGC 1 cut(s) 844
MaeI CTAG 1 cut(s) 546
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 4 cut(s) 37, 296, 664, 844
MalI GATC 8 cut(s) 183, 386, 398, 419, 476, 975, 990, 1053
MboI GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
MflI RGATCY 2 cut(s) 181, 1051
MhlI GDGCHC 1 cut(s) 939
MluCI AATT 1 cut(s) 744
MlyI GAGTC 2 cut(s) 34, 421
MnlI CCTC 9 cut(s) 90, 104, 332, 365, 623, 791, 837, 896, 1074
MseI TTAA 6 cut(s) 74, 200, 266, 501, 822, 896
MslI CAYNNNNRTG 1 cut(s) 631
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 148
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 386
NdeII GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
NlaIII CATG 6 cut(s) 71, 427, 630, 732, 875, 964
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 3 cut(s) 37, 664, 844
NspI RCATGY 1 cut(s) 732
PagI TCATGA 1 cut(s) 960
PciI ACATGT 1 cut(s) 728
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 9 cut(s) 16, 355, 448, 461, 700, 967, 1009, 1022, 1060
PfoI TCCNGGA 1 cut(s) 146
PkrI GCNGC 1 cut(s) 859
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 2 cut(s) 34, 421
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
PpsI GAGTC 2 cut(s) 34, 421
Ppu21I YACGTR 1 cut(s) 516
PpuMI RGGWCCY 1 cut(s) 800
PscI ACATGT 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 800
Psp6I CCWGG 1 cut(s) 146
PspCI CACGTG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 4 cut(s) 440, 561, 772, 800
PspPPI RGGWCCY 1 cut(s) 800
PsuI RGATCY 2 cut(s) 181, 1051
PvuI CGATCG 1 cut(s) 477
RsaI GTAC 1 cut(s) 835
RsaNI GTAC 1 cut(s) 834
RseI CAYNNNNRTG 1 cut(s) 631
SaqAI TTAA 6 cut(s) 74, 200, 266, 501, 822, 896
SatI GCNGC 1 cut(s) 858
Sau3AI GATC 8 cut(s) 181, 384, 396, 417, 474, 973, 988, 1051
Sau96I GGNCC 4 cut(s) 440, 561, 772, 800
SchI GAGTC 2 cut(s) 34, 421
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 1 cut(s) 939
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 4 cut(s) 440, 561, 772, 800
SmiMI CAYNNNNRTG 1 cut(s) 631
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 1 cut(s) 744
SsiI CCGC 1 cut(s) 465
SspI AATATT 1 cut(s) 204
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 146
TaaI ACNGT 4 cut(s) 772, 819, 838, 934
TaiI ACGT 1 cut(s) 518
TasI AATT 1 cut(s) 744
TatI WGTACW 1 cut(s) 833
TfiI GAWTC 9 cut(s) 16, 355, 448, 461, 700, 967, 1009, 1022, 1060
Tru1I TTAA 6 cut(s) 74, 200, 266, 501, 822, 896
Tru9I TTAA 6 cut(s) 74, 200, 266, 501, 822, 896
TscAI CASTG 4 cut(s) 297, 822, 892, 937
TseFI GTSAC 3 cut(s) 37, 664, 844
TseI GCWGC 1 cut(s) 857
Tsp45I GTSAC 3 cut(s) 37, 664, 844
TspDTI ATGAA 6 cut(s) 56, 112, 261, 888, 980, 1001
TspRI CASTG 4 cut(s) 297, 822, 892, 937
VpaK11BI GGWCC 4 cut(s) 440, 561, 772, 800
XceI RCATGY 1 cut(s) 732
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.