AT4G30990

Down-regulated in metastasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005459
Physical Location & Seq
Forward (+)
15084098 .. 15097986
13889 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30990.1

Sequence Viewer

Length: 7800 bp
ATGGCGACATCAGCTGATGCTCGGGCAGTAAAGTCGCTCAACACTTCCGAGGGGCGTAAGAGATTTGTGTTCAAGTCTGCTTCTCAACGAACAAATGACATTGACAACATCAGCAACTATAGGAACTTGGATAAGGTGAAGGCTGAGCCTTCAGAAGGATCCACATTTTTCAGGGATTGCCTCATAGAATGGAGGGAACTGAATACCGCAGAAGATTTCATTTTGTTTTATGAAGAGATGTTGCCATCTGTCCAGAGTTTGAGTTTGATTATTATGCAAAAAGAAAGAATCTTCTCAAATCTTGTCTCTCGATTACAAATGAAAGCAAGATTGTCTCTGGAACCCATTCTCAGGTTGATTGCTGCTCTATCAAGAGATCTCTTGAATGACTTTATTCCCTTCTTGCCACAAATTGTGAACTCCTTCGTGACTCTCCTAAATAATGGTGCGCATAACGATCCGGAGATTATTCAGCAGGTATTCACATCATGGGCTTCCATAATAGTGTCCCTGCAGAAGTATCTTGTGTGCGACATCGAAGGCATTCTCAGGGATACGTTGGAATTGAGGTACTACCCCAAAGACAATATCAGTGAATTTATGTCAGAGTCCATGTCATTTTTGTTGAGGAAAGCACAGGATGAGCAACTTGAAAAAGGTATGAAGATGATCCTTTCTGAAGTTGCACATCCATCCAAAAAAGCTGGAGGAGTTGGAGTACTATATAATGTTATGAGGGGTACCTATGGAAGACTCCATTCAAAAGCCGGGAGAGTTTTGAGCTTCTTGCTGAAAGATTCAACATTATCTTTTTTGGACAATTTTCCTCAAGGCCCTTGTACAGTCGTGGAAGTTGTGAGTTTGGTTTTGCAGAGAATATGCGAGGATTTGGAAGCAGAAAAATTAAGTGCTATGTGGGAATACTTGTATAAGAAAATAAACAAATCAATCTCAAACAAGAAATCAGTTCATCTAAGCCGGCTGTTGAGTGTGCTTATGGCGGTTGTTAAGATTAAGGAAGGTCGCAAGGTTCACGATATCCCATCACTGATTGGAATCGTGAGCCGCATTGTGTCAACTTTTTTCACTTCCTCTGAGACCGCTGTAGAAGGGGATAACTTATCTGCTGTCCTTGATGAAGTTTTGGAATTGATATTATGCACAATCAACACGGTCAATGAAATGGAAACTGTTGCTTCGCTTTGGGCTCCCATTTTTGCCTTGAAGAGTTCAAGTTTGCTGACTTTTTTGCGGGAGTTCCTAAAGAAAGATCAATCAGTAGTGAAAGCCTTTACAAAGAATATATTATGTGCAATTAACAACATGATTTGGGAGTCTTCTGAGGAAGTTATTCCTCTGTTACTAACATTGTGTGAAGAGCATAAGACACAACAAACAAGTCACGACGTCGTGAACAGCATAAGTCAAACATTTGAGAGTAGATACGAGAGAATTCATGAGTTCTTGGAAGCAAAGATTAAAAAGGTTCAACAGAATATTGAGAATGCTGGATTAGCTCAAATTAATGAGGCCGAGTTGGCTGCAATTTGGGGAGTTGTCAAGTGTTATCCTTATTTTAAAGTGGATTCATCATTATTGATTTGCTTTAAGAAAACTCTCAGACAGCATTTGGCAGTGTCAGATGTAGACACTTGCAGTGGCCCAGAATTGATGTGGCAGAGCTTACTTGGTACTACTTTGAGGTCATGTTACAAAATGACGGGAATCAACCACAGTGATCTAGAGGAAGCTTTGTCTTTTGCTAAAGATTACAAGTCATGTGAGCAAGTGTTGTCCCCGGTGGCCGATGTTTTGGAATTTATGCACAGGCCTGCATTAGCTCATGGTAGGTCTAAGCCTTATCCAGAACTTCAAGCAAATAAGGCTGGAGATGCTTTTGAAATATTTTCTGAAAATTTGCGTCATCCGAACAAAAATATCCGTCTTATGACTCTGAGGATTTTGTGCCATTTTGAAACTTTGTCGTCTGACCCTTCTTTTGAAGAGCATCCTCCTAAGAAGAAAATGAAAACTGAGAAGAATGTTCTTCAGTTATTACTCTTATTCGAAGAGACTGCTCCCACAGTGGATACTAGTCGGATGTTGGCAGGATACATTTCTACAATACAAGATAACCTCTCCGCTGGCAGGATACATTCGGCATATGTGAAGCTTGTATTGAATGGGATGTTGGGGATATTACATATTAGTTATCGTCCTTTGTGTGTTCAAGCATCTGAGTGTCTTGCGGTTCTTGTGAGGAAATATACTGGTGCAGTGTGGAGTGATTTTGTTTGTTATTTGGGGCAGTGCCAACTAAAATTTGAAACCCTCCATGATCATAGTGAGAATGCGAACCAAAGTATGTCAGAGAGGCATGCATGTAATTTAAATCTGAATGGCCGTTTTAATTTGTTTCTCTTTCCACCATCTGCTATCACGCCTACTGCAACGGTATCTGATGTGGTATCTCAGTTGCTACAGACCTTACAAAAAGCTTCAAGTGTTGCTCAGTCTCGGGCTTCTGAAATTCTCCCTTTGTTGTTAAAATTTCTGGGATACAACAGCGAAAATCCTGGGAGTGTCGGATCATATAATGGCCGAGTTTGTAAAGGAGAGGACTGGAAGACGGTTCTTGTACAATGGTTGACGTTGCTAAAATTAATGAAGAATCCTAGGTCGTTTTGTTTTAGTCAATTTTTAAACGACATTCTGCAGAATAGGTTCCTGGATGATAATGATGCTGAAATCCAAACGAATGTTCTAGAGTGCCTTCTGTTGGCGAATGACTTCTTACTCCCACACCGCCAGCATTTGTTGAATTTGATCAAACCAAAAGAATTGAGAGAAGAACTCACAACCTGGAACTTGTCCGAAAACATCGGAGAACCTCACAGATCTTATATTTTTTCTCTTGTAATTCGCATCCTTATGCCAAAAGTTAGGACATTAAAAAATTCGGCTTCAAGGAAGCATACAAGTATCCGTCACCGGAAGGCAGTTCTTTGCTTTATATCTCAGCTGGATGTTAATGAACTTGCGTTGTTCTTTGCATTGTTGATAAAACCTTTGAACATCATATCAGAGGAAACAATGGACTCATTTTGGAGTTCAGGCAAAAGTTCTCTCGACTATTTCCAAAACTCCAATTTTCTGAAATATTTCACTGTTGATACTATTTCAACGTTATCCAGAAATCAGAAATTTGGGTTTCTTCATGTCATCCAACATATCCTTGAAGTCTTTGATGAACTTCGTGTCCGACCTTTTCTAGACTTTATGATGGGATGTGTTGTCCGGCTATTGGTAAATTATGCTCCAAATGTTGATGAAGAAATGAACATTGATTCTTTGGCACTAAGAAATGTCACTGCTGCCCCATCAACTTCGGATGATAAAGAAAATGCTTCAATAAATCACGACCAGGCTGGCACTGCTTTTAAGCAGTTTAAAGAGTTGAGATCCTTATGTCTGAAAATCATTGCTCATGTTCTTGATAAATACGAGGATTGTGATCTTGGCTCCGAGTTCTGGGACCTCTTCTTTTCAGCTGTGAGTCCGTTAATTAAGAGTTTCAAGCAGGAGGGTTCTAGTAGTGAGAAACCAAGTTCCTTGTTCTCATGCTTCTTGTCAATGAGTAAAAGCCGTAATCTCGTGAATCTCTTATGTCGGGAAGAGTCCCTTGTTCCAGACATTTTTTCAATTCTAACTGTCACCACGGCTTCAGAAGCTATAAAGTCTTCTGCCTTGAAGTTCATTGAGAATCTGCTTTGTCTTGACAATGTGTTGGGTGAGGATGAAAATATGATCCGTGGATTCGTAGATCCGTATATAGAAGCACTGATTAACAGCTTGCATTCTCTTTTCATTGGGGATATTTTGAAAAGGAAATCAGTCAAGTATCATGGGGAAAGAGAGATTAAAATTCTTAAATTATTGTCAAAGCGTATGCAAGATCGCTCCCATGTTATGAAGTATTTGGATGTCTTGCTGTCTTTCTTGAATAAAAGTGTTAAAGATCCTGATATCCGTCGTGAAGCTTTGCTAGCCATTCAGGACATCATAGCGTATCTTGGGATGGAGAGTACCAGCAAGATTATAAATACAGTCTCTCCTCTACTTGTTGATGCTGAACTTGATGTTAGATTATGCATTTGTGATCTTCTTGAGTCTCTTGCAAAAATCGACTTTTCACTGGATGATGTGGCAAAGCGTGTCCGCGATATGAATGCCATCTCAGCTATGGAAGTTGATGACCTTGACTATGAAAAGATAGTTAATGCCTATGTGGAGATTAATGCAGATTTCTTTATTAAATCCTCAGAGCAGCATACGATGATTATACTCTCACAGTCCTCAATACTTTGCCGAGAAGCTCCAGCTCACTCTGAATTTGGAAAAGAGGTCAAAAACGCTGATGTAAGCTGGACAGGAGATCGTGTACTGTGCATTTTGAGAAATTTTATTTTGAAACACATTGGAGATGCAATAAACAGAGGGGGCATTATAATAAAGGAATGGATTCTTTTGATACGCGAGATGGTGACCAAACTTCCAGATGCCGCAAATCTTTCTGCATTCAGACCTCTATGCTCTGAAGACGAGAATGTAGATTTTTTTAAAGCTATTGTTCACATTCAGGCACACCGTAGAGCTAGGGCAATTTCACGTTTCAGTAGTGTGGTTAAAGATAGCAGTCTGCCTGAGGGAGTAGTGAGAAAACTTTTGGTCTCGGTCTTTTTCAATATGTTACTCGAAGGACAAGATGGAAAAGATAATAATGTCAGAAATGCATGCACAGAAGCCCTTGCATCTATATCTGCTCATATGAGTTGGACATCGTACTATGCTTTACTGAATCGGTGTTTCCGAGAGATGAACAAGCACACCAAAAAAGGAAAAATTCTGCTGCGACTTATCTGCTTGATTTTGGATAAATTTCATTTTGCAAAAGATGGCTACCCACATGAGGCTGAGGAAATTAGGACTTGCCTTCAGAAAATTGTGTTCCCGAGGATGCAGAAGCTGATGAATTCTGATTCTGATAATGTTAATGTTAACAGTAGTGTGGCTGCGCTTAAGGTTCTAAAGCTACTCCCTGAAGATGTATTGGACTCAAACCTATCATCTATAGTTCACAAAATTGCCAGTTTTTTAAAGAACCGGTTGGAGAGCACACGTGATGAAGCCAGATTGGCTCTGGTTGCTTGTTTGAAAGAACTTGGGCTGGAGTACTTGCAAGTTGTAGTCAACATTTTACGTGCTATCTTAAAACGAGGATCTGAGGTGCATGTGCTGGGGTACACTCTCAATTCTATCTTATCAAAGTGCTTGTCCAATCCTACATGTGGGAAGTTAGATCATTGTTTGGTTGATCTCCTTGCTGTGGTGGAAACCGATATCCTTGGAGAGGTTGCTGAACAGAAAGAGGTGGAAAAATTTGCATCCAAAATGAAAGAAACAAGAAAACGGAAGTCATTTGAGACTCTGAAATTGATTGCTGAAAATGTAACGTTCAGAAGCCATGGATTAAAGTTACTTTCTCCGGTCACTGCTCAGCTGCAGCGGCATCTAACTCCAAAGATCAAAACAAATCTGGAGAAGATGTTAAAACAAATTGCAGCTGGTATTGAAGGCAATACATCTGTTGACCAAGGAGATCTTTTTCTTTTTATATATGGCCTAGTTGATGATGGTATTAATAACAGGAGTGGTCTTGGAGATCAAGTGTCTTTACCACCCTCCAAAAAGAAGAAGAAATCAAGAGATCTGAAAGAGACTTCTGGGTTGTGTTTTGGTCCGAAGTCATGTCCACATCTTATAACCGTGTTTGCTCTGGACTTATTTTATAACCGAATGAAAAAATTGAGACTCGACAACACTGATGAAGAGCTACTGTCGAAATGTTTTACTTCATTAGTAAAGTTTCCACTGCCTTCCCTCACGTCTGAAGCAGATGAACTGAAAACAGCATTGTTAACCATTGCTCAGTCTGCAGTTAGTTCCAGCAGTCCTCTTGTGCAGTCATGCCTTAAGCTGCTTACAACGCTTCTCAAAAATATAAATATAACTTTATCATCTGAGCAGTTGAAAATGTTGATTCAGTTCCCCATATTTATTGATCTGGAGTCTGATTCATCCTTTGTTACTCTTTCACTTCTCAAGGCCATCATGAACCGGAAGCTTGTAGTTCCAGAGATATATGACATTGCAATTCAGGTTTCAAAGCTGATGGTAAATAGTCAGTTGGAATCAATCCGCAAGAAATGTAAACATATACTGTTGCAATTTATGGTCCACTATACACTTTCTGAAAAACGTTTAGAGCAACATGTGAACTTTCTGCTGGAAAATCTGAGGTATGAGTTTCCAACTGGAAGAGAAGCAGTTCTTGACATGCTTCATGCCCTAATCTTAAAATTCTCAGAACCTAATCTTGGCAAGCAGTCTGTTCTTGACCAGCAGTCGCAAAAATTGTTTATTCAACTTACTGTCTGTTTGTCCAATGAAACTGATAGAAAAGTGCTGCCCTTGGTTGGTGCTGTGATAGAAGTTCTGATAGGCCGCATGAGTAAAGATCAAGTTGATTCCAGTCTCTTGTACTGTTTGTGTTGGTATAAGCAGCAGAACTTAAGTGCTGCGGCGGCACAGGTATTGGGATTTTTTATCAGCGCCATGAAGAAAACATTTCGGAAGCATATCTACAATACAGTGGAGGATGCTAGAACTATTTTGGAGTCTGCAATTAGTGCTTCCAGCTTACAGTTGCAAGATACTGTTGAGGAAGCCAGTCTTCCGTTCTGGAAGGAAGCATACTACTCACTGGTTATGATAGAAAAGATGCTAGAGCAATTTCCTGATTTAAGATTCGGAAAAGATTTAGAGGATATTTGGAAAATGGTGTTTAAGTTCTTGTTGCACCCACACGCATGGTTGCGAAATAAATCCTGCAGGCTCCTGAATCTTTATTTTGAGGCATTAGCTGGGAGAAAAAGACCAGAATGTCGTACATTAGTAGCGGATTCTCTCCTAGAGAAGCCAAGTAGTCTGTTCATGGTGGCTGTTTCTCTTTGCTTCCAGTTAAAGGAGCAACCCACCACGGGCAACATTGATGTTGATCTCCTCACAGCAAATATTGTTTTTGCGGTCTCTAGTCTTCATTCTCTGATTGGACAATTTGATCAGGCAACCCACAATCGGTTCTGGTCCAGTCTTGGTGAGGATGAGCAAGTGGTATTCCTCAAAGCCTTTGAAGTGCTTGATGCAGGGAAAGGGAGGTCTACTTTTTTGGCTCTTACCTCAGGCAAACGTACTGAGAATGGTGATGATGATGTAAGAAATGTAATGATCGGAAGTTTGCTCAAAAGAATGGGGAAGATTGCTCTTGATATGGAGTCTGTTCAGATGAGAGTTATGTTCAACGTCTATAAATCTTTTGCCTCACAATTGAATCAAGAAGAATGTCGCTTATATGCCTACAAAATTCTGCTTCCGTTGTACAAAGTCTGCGAAGGATATACGGGAAAAATCGTCACAGATGAGTTGAAACAGCTAGCAGAAGAGGTACGAGACAGTATAAGGGACAAGAGTTTAGGCAACAAAATGTTTGTGGAAGTATACAGTGAAATAAGAAACAGTTTGAGAACGAAAAGAGACAAGAGAAAGCGAGAAGAGAAGCTAATGGCAGTGGTAAATCCGGAGAGAAACGCCAAGAGGAAACTGAGGTTGGCTTCAAAGAATAAAGCAAACAAGAAAAGGAGAATGACGAGCATGAAATTGTCTAGATGGGCTTGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRIM

Protein Analysis

2599

Amino Acids

294.8

Weight (kDa)

8.4

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UTP20_N PF07539 876 - 1451 7.1e-90 U3 small nucleolar RNA-associated protein 20, N-terminal
UTP20 PF20416 1680 - 1896 2e-60 U3 small nucleolar RNA-associated protein 20 domain
UTP20_C PF23099 2471 - 2588 6.8e-09 Small subunit processome component 20 homolog, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 4100, 4508, 5822, 5850
AasI GACNNNNNNGTC 1 cut(s) 7002
AatII GACGTC 1 cut(s) 1410
Acc16I TGCGCA 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 740
AccB1I GGYRCC 1 cut(s) 740
AccI GTMKAC 3 cut(s) 1647, 7280, 7617
AccII CGCG 2 cut(s) 4221, 4536
AccIII TCCGGA 2 cut(s) 460, 7696
AclI AACGTT 3 cut(s) 3185, 5513, 6319
AcoI YGGCCR 3 cut(s) 1803, 2401, 2599
AcuI CTGAAG 8 cut(s) 135, 699, 2033, 3708, 4617, 4979, 5121, 5970
AcvI CACGTG 1 cut(s) 5180
AcyI GRCGYC 1 cut(s) 1407
AflII CTTAAG 3 cut(s) 5078, 6032, 6631
AflIII ACRYGT 3 cut(s) 5177, 5345, 6331
AgeI ACCGGT 1 cut(s) 5163
AhdI GACNNNNNGTC 1 cut(s) 6463
AhlI ACTAGT 1 cut(s) 2093
AjiI CACGTC 1 cut(s) 5946
AjnI CCWGG 4 cut(s) 2575, 2727, 2861, 3423
AjuI GAANNNNNNNTTGG 8 cut(s) 2174, 2206, 2745, 2777, 6420, 6452, 7709, 7741
AloI GAACNNNNNNTCC 2 cut(s) 3243, 3275
Alw21I GWGCWC 1 cut(s) 5177
AlwNI CAGNNNCTG 1 cut(s) 5026
Ama87I CYCGRG 3 cut(s) 21, 2517, 5011
Aor13HI TCCGGA 2 cut(s) 460, 7696
ArsI GACNNNNNNTTYG 4 cut(s) 1982, 2014, 5318, 5350
AseI ATTAAT 4 cut(s) 1524, 2663, 4296, 5700
AsiGI ACCGGT 1 cut(s) 5163
Asp700I GAANNNNTTC 9 cut(s) 345, 422, 543, 1350, 2723, 2789, 3161, 4521, 6387
Asp718I GGTACC 1 cut(s) 740
AspA2I CCTAGG 1 cut(s) 2675
AspLEI GCGC 3 cut(s) 451, 5077, 6674
AspS9I GGNCC 6 cut(s) 833, 1661, 3535, 5798, 6295, 7206
AsuC2I CCSGG 2 cut(s) 769, 1799
AsuHPI GGTGA 7 cut(s) 148, 2981, 3706, 3803, 4555, 7229, 7334
AsuII TTCGAA 1 cut(s) 2067
AsuNHI GCTAGC 2 cut(s) 4045, 7552
AvaI CYCGRG 3 cut(s) 21, 2517, 5011
AvaII GGWCC 4 cut(s) 3535, 5798, 6295, 7206
AvrII CCTAGG 1 cut(s) 2675
AxyI CCTNAGG 2 cut(s) 4704, 7300
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 740
BanII GRGCYC 1 cut(s) 1210
BarI GAAGNNNNNNTAC 4 cut(s) 6686, 6718, 6797, 6829
BauI CACGAG 1 cut(s) 3653
BbrPI CACGTG 1 cut(s) 5180
BbsI GAAGAC 7 cut(s) 757, 1329, 2633, 3732, 4605, 6785, 7148
Bbv12I GWGCWC 1 cut(s) 5177
BbvCI CCTCAGC 1 cut(s) 4974
BceAI ACGGC 3 cut(s) 2388, 3630, 3735
BciT130I CCWGG 4 cut(s) 2577, 2729, 2863, 3425
BciVI GTATCC 6 cut(s) 547, 2083, 2105, 2145, 2552, 2993
BclI TGATCA 3 cut(s) 2338, 2826, 7180
BcnI CCSGG 2 cut(s) 769, 1799
BcuI ACTAGT 1 cut(s) 2093
BfmI CTRYAG 9 cut(s) 118, 512, 1104, 2480, 2714, 5130, 5561, 5994, 6949
BfoI RGCGCY 1 cut(s) 6675
BfrI CTTAAG 3 cut(s) 5078, 6032, 6631
BfuAI ACCTGC 1 cut(s) 466
BfuI GTATCC 6 cut(s) 547, 2083, 2105, 2145, 2552, 2993
BglI GCCNNNNNGGC 2 cut(s) 1538, 5195
BglII AGATCT 4 cut(s) 376, 2897, 5659, 5767
BlnI CCTAGG 1 cut(s) 2675
BlpI GCTNAGC 2 cut(s) 144, 5556
BmcAI AGTACT 2 cut(s) 720, 5234
Bme1390I CCNGG 6 cut(s) 769, 1799, 2577, 2729, 2863, 3425
Bme18I GGWCC 4 cut(s) 3535, 5798, 6295, 7206
BmeRI GACNNNNNGTC 1 cut(s) 6463
BmeT110I CYCGRG 3 cut(s) 21, 2517, 5011
BmgBI CACGTC 1 cut(s) 5946
BmgT120I GGNCC 6 cut(s) 833, 1661, 3535, 5798, 6295, 7206
BmiI GGNNCC 8 cut(s) 160, 342, 742, 1209, 2726, 3523, 3536, 6956
BmrFI CCNGG 6 cut(s) 769, 1799, 2577, 2729, 2863, 3425
BmtI GCTAGC 2 cut(s) 4049, 7556
BpiI GAAGAC 7 cut(s) 757, 1329, 2633, 3732, 4605, 6785, 7148
BplI GAGNNNNNCTC 2 cut(s) 2838, 2870
BpmI CTGGAG 6 cut(s) 726, 1908, 4362, 5249, 5618, 6146
Bpu10I CCTNAGC 1 cut(s) 4974
Bpu1102I GCTNAGC 2 cut(s) 144, 5556
Bpu14I TTCGAA 1 cut(s) 2067
BpuEI CTTGAG 3 cut(s) 813, 4187, 6146
BpuMI CCSGG 2 cut(s) 769, 1799
BsaAI YACGTR 2 cut(s) 5180, 5261
BsaBI GATNNNNATC 3 cut(s) 1055, 3513, 7116
BsaHI GRCGYC 1 cut(s) 1407
BsaI GGTCTC 3 cut(s) 1091, 4735, 7153
BsaWI WCCGGW 6 cut(s) 460, 2991, 5163, 5545, 6177, 7696
Bse118I RCCGGY 2 cut(s) 978, 5163
Bse21I CCTNAGG 2 cut(s) 4704, 7300
Bse3DI GCAATG 3 cut(s) 3480, 5982, 6207
Bse8I GATNNNNATC 3 cut(s) 1055, 3513, 7116
BseAI TCCGGA 2 cut(s) 460, 7696
BseBI CCWGG 4 cut(s) 2577, 2729, 2863, 3425
BseJI GATNNNNATC 3 cut(s) 1055, 3513, 7116
BseMI GCAATG 3 cut(s) 3480, 5982, 6207
BseRI GAGGAG 3 cut(s) 723, 4104, 7112
BseYI CCCAGC 2 cut(s) 5296, 6983
BsgI GTGCAG 2 cut(s) 2295, 6041
Bsh1236I CGCG 2 cut(s) 4221, 4536
BshNI GGYRCC 1 cut(s) 740
BshTI ACCGGT 1 cut(s) 5163
BsiHKAI GWGCWC 1 cut(s) 5177
BsiHKCI CYCGRG 3 cut(s) 21, 2517, 5011
BslFI GGGAC 5 cut(s) 493, 1780, 3548, 3664, 7595
BsmFI GGGAC 5 cut(s) 493, 1780, 3548, 3664, 7595
BsmI GAATGC 6 cut(s) 543, 1510, 2356, 3856, 4234, 4577
Bso31I GGTCTC 3 cut(s) 1091, 4735, 7153
BsoBI CYCGRG 3 cut(s) 21, 2517, 5011
Bsp119I TTCGAA 1 cut(s) 2067
Bsp1286I GDGCHC 2 cut(s) 1210, 5177
Bsp13I TCCGGA 2 cut(s) 460, 7696
Bsp1407I TGTACA 3 cut(s) 839, 2638, 7497
Bsp1720I GCTNAGC 2 cut(s) 144, 5556
Bsp19I CCATGG 1 cut(s) 5524
BspEI TCCGGA 2 cut(s) 460, 7696
BspFNI CGCG 2 cut(s) 4221, 4536
BspHI TCATGA 2 cut(s) 1456, 6171
BspLI GGNNCC 8 cut(s) 160, 342, 742, 1209, 2726, 3523, 3536, 6956
BspMAI CTGCAG 5 cut(s) 516, 2718, 5565, 5998, 6953
BspMI ACCTGC 1 cut(s) 466
BspOI GCTAGC 2 cut(s) 4049, 7556
BspQI GCTCTTC 3 cut(s) 1371, 1998, 5883
BspT104I TTCGAA 1 cut(s) 2067
BspT107I GGYRCC 1 cut(s) 740
BspTI CTTAAG 3 cut(s) 5078, 6032, 6631
BspTNI GGTCTC 3 cut(s) 1091, 4735, 7153
BsrDI GCAATG 3 cut(s) 3480, 5982, 6207
BsrFI RCCGGY 2 cut(s) 978, 5163
BsrGI TGTACA 3 cut(s) 839, 2638, 7497
BssAI RCCGGY 2 cut(s) 978, 5163
BssNAI GTATAC 1 cut(s) 7618
BssNI GRCGYC 1 cut(s) 1407
BssSI CACGAG 1 cut(s) 3653
BssT1I CCWWGG 5 cut(s) 2675, 5404, 5524, 5653, 6531
Bst1107I GTATAC 1 cut(s) 7618
Bst2BI CACGAG 1 cut(s) 3653
Bst2UI CCWGG 4 cut(s) 2577, 2729, 2863, 3425
BstACI GRCGYC 1 cut(s) 1407
BstAFI CTTAAG 3 cut(s) 5078, 6032, 6631
BstAPI GCANNNNNTGC 1 cut(s) 6749
BstAUI TGTACA 3 cut(s) 839, 2638, 7497
BstBAI YACGTR 2 cut(s) 5180, 5261
BstBI TTCGAA 1 cut(s) 2067
BstDSI CCRYGG 4 cut(s) 3717, 3811, 5524, 7098
BstEII GGTNACC 1 cut(s) 4543
BstENI CCTNNNNNAGG 2 cut(s) 153, 5408
BstFNI CGCG 2 cut(s) 4221, 4536
BstH2I RGCGCY 1 cut(s) 6675
BstHHI GCGC 3 cut(s) 451, 5077, 6674
BstNI CCWGG 4 cut(s) 2577, 2729, 2863, 3425
BstNSI RCATGY 7 cut(s) 2381, 2385, 4797, 5294, 5349, 6335, 6400
BstPI GGTNACC 1 cut(s) 4543
BstSCI CCNGG 6 cut(s) 767, 1797, 2575, 2727, 2861, 3423
BstSFI CTRYAG 9 cut(s) 118, 512, 1104, 2480, 2714, 5130, 5561, 5994, 6949
BstUI CGCG 2 cut(s) 4221, 4536
BstV2I GAAGAC 7 cut(s) 757, 1329, 2633, 3732, 4605, 6785, 7148
BstX2I RGATCY 9 cut(s) 158, 376, 2897, 3461, 3823, 4018, 5279, 5659, 5767
BstXI CCANNNNNNTGG 1 cut(s) 6930
BstYI RGATCY 9 cut(s) 158, 376, 2897, 3461, 3823, 4018, 5279, 5659, 5767
BstZ17I GTATAC 1 cut(s) 7618
Bsu36I CCTNAGG 2 cut(s) 4704, 7300
BsuI GTATCC 6 cut(s) 547, 2083, 2105, 2145, 2552, 2993
BtgI CCRYGG 4 cut(s) 3717, 3811, 5524, 7098
BtrI CACGTC 1 cut(s) 5946
BtsI GCAGTG 9 cut(s) 1641, 1663, 2283, 2315, 3369, 3432, 5550, 5930, 7692
BveI ACCTGC 1 cut(s) 466
CaiI CAGNNNCTG 1 cut(s) 5026
CciI TCATGA 2 cut(s) 1456, 6171
CfoI GCGC 3 cut(s) 451, 5077, 6674
Cfr10I RCCGGY 2 cut(s) 978, 5163
Cfr13I GGNCC 6 cut(s) 833, 1661, 3535, 5798, 6295, 7206
CseI GACGC 1 cut(s) 1910
CspAI ACCGGT 1 cut(s) 5163
DraI TTTAAA 6 cut(s) 1579, 2391, 2703, 3451, 4621, 5157
DrdI GACNNNNNNGTC 1 cut(s) 7002
DriI GACNNNNNGTC 1 cut(s) 6463
DseDI GACNNNNNNGTC 1 cut(s) 7002
EaeI YGGCCR 3 cut(s) 1803, 2401, 2599
Eam1105I GACNNNNNGTC 1 cut(s) 6463
Eco130I CCWWGG 5 cut(s) 2675, 5404, 5524, 5653, 6531
Eco147I AGGCCT 1 cut(s) 1831
Eco24I GRGCYC 1 cut(s) 1210
Eco31I GGTCTC 3 cut(s) 1091, 4735, 7153
Eco32I GATATC 3 cut(s) 1039, 4027, 5401
Eco47I GGWCC 4 cut(s) 3535, 5798, 6295, 7206
Eco57I CTGAAG 8 cut(s) 135, 699, 2033, 3708, 4617, 4979, 5121, 5970
Eco72I CACGTG 1 cut(s) 5180
Eco81I CCTNAGG 2 cut(s) 4704, 7300
Eco88I CYCGRG 3 cut(s) 21, 2517, 5011
Eco91I GGTNACC 1 cut(s) 4543
EcoNI CCTNNNNNAGG 2 cut(s) 153, 5408
EcoO109I RGGNCCY 2 cut(s) 833, 3535
EcoO65I GGTNACC 1 cut(s) 4543
EcoRI GAATTC 2 cut(s) 1452, 5032
EcoRII CCWGG 4 cut(s) 2575, 2727, 2861, 3423
EcoRV GATATC 3 cut(s) 1039, 4027, 5401
EcoT14I CCWWGG 5 cut(s) 2675, 5404, 5524, 5653, 6531
EcoT22I ATGCAT 3 cut(s) 2383, 4154, 4795
EcoT38I GRGCYC 1 cut(s) 1210
ErhI CCWWGG 5 cut(s) 2675, 5404, 5524, 5653, 6531
FalI AAGNNNNNCTT 8 cut(s) 3666, 3698, 5646, 5678, 6375, 6407, 6777, 6809
FaqI GGGAC 5 cut(s) 493, 1780, 3548, 3664, 7595
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 2 cut(s) 2164, 4827
FbaI TGATCA 3 cut(s) 2338, 2826, 7180
FblI GTMKAC 3 cut(s) 1647, 7280, 7617
FriOI GRGCYC 1 cut(s) 1210
FspAI RTGCGCAY 1 cut(s) 450
FspI TGCGCA 1 cut(s) 450
GlaI GCGC 3 cut(s) 450, 5076, 6673
GsaI CCCAGC 2 cut(s) 5300, 6987
GsuI CTGGAG 6 cut(s) 726, 1908, 4362, 5249, 5618, 6146
HaeII RGCGCY 1 cut(s) 6675
HgaI GACGC 1 cut(s) 1910
HhaI GCGC 3 cut(s) 451, 5077, 6674
Hin1I GRCGYC 1 cut(s) 1407
Hin6I GCGC 3 cut(s) 449, 5075, 6672
HinP1I GCGC 3 cut(s) 449, 5075, 6672
HincII GTYRAC 6 cut(s) 1077, 2649, 5059, 5251, 5650, 5979
HindII GTYRAC 6 cut(s) 1077, 2649, 5059, 5251, 5650, 5979
HindIII AAGCTT 5 cut(s) 1749, 2171, 2496, 4038, 6182
HpaI GTTAAC 2 cut(s) 5059, 5979
HphI GGTGA 7 cut(s) 148, 2981, 3706, 3803, 4555, 7229, 7334
Hpy99I CGWCG 3 cut(s) 1409, 1412, 4035
Hsp92I GRCGYC 1 cut(s) 1407
HspAI GCGC 3 cut(s) 449, 5075, 6672
Kpn2I TCCGGA 2 cut(s) 460, 7696
KpnI GGTACC 1 cut(s) 744
KroI GCCGGC 1 cut(s) 978
KroNI GCCGGC 1 cut(s) 980
Ksp22I TGATCA 3 cut(s) 2338, 2826, 7180
KspAI GTTAAC 2 cut(s) 5059, 5979
LguI GCTCTTC 3 cut(s) 1371, 1998, 5883
LmnI GCTCC 8 cut(s) 1213, 2083, 3322, 3527, 3965, 4381, 6960, 7087
MfeI CAATTG 1 cut(s) 7445
MflI RGATCY 9 cut(s) 158, 376, 2897, 3461, 3823, 4018, 5279, 5659, 5767
MhlI GDGCHC 2 cut(s) 1210, 5177
Mph1103I ATGCAT 3 cut(s) 2383, 4154, 4795
MroI TCCGGA 2 cut(s) 460, 7696
MroNI GCCGGC 1 cut(s) 978
MroXI GAANNNNTTC 9 cut(s) 345, 422, 543, 1350, 2723, 2789, 3161, 4521, 6387
MslI CAYNNNNRTG 4 cut(s) 503, 2239, 2930, 6928
MspA1I CMGCKG 8 cut(s) 14, 1103, 2144, 3022, 3551, 5560, 5566, 5624
MspCI CTTAAG 3 cut(s) 5078, 6032, 6631
MspR9I CCNGG 6 cut(s) 769, 1799, 2577, 2729, 2863, 3425
MunI CAATTG 1 cut(s) 7445
Mva1269I GAATGC 6 cut(s) 543, 1510, 2356, 3856, 4234, 4577
MvaI CCWGG 4 cut(s) 2577, 2729, 2863, 3425
MvnI CGCG 2 cut(s) 4221, 4536
NaeI GCCGGC 1 cut(s) 980
NciI CCSGG 2 cut(s) 769, 1799
NcoI CCATGG 1 cut(s) 5524
NdeI CATATG 2 cut(s) 2164, 4827
NgoMIV GCCGGC 1 cut(s) 978
NheI GCTAGC 2 cut(s) 4045, 7552
NlaIV GGNNCC 8 cut(s) 160, 342, 742, 1209, 2726, 3523, 3536, 6956
NmeAIII GCCGAG 3 cut(s) 1558, 2627, 4394
NmuCI GTSAC 8 cut(s) 427, 1400, 2987, 3367, 3712, 4543, 5548, 7531
NsbI TGCGCA 1 cut(s) 450
NsiI ATGCAT 3 cut(s) 2383, 4154, 4795
NspI RCATGY 7 cut(s) 2381, 2385, 4797, 5294, 5349, 6335, 6400
NspV TTCGAA 1 cut(s) 2067
PacI TTAATTAA 1 cut(s) 3567
PaeI GCATGC 2 cut(s) 2381, 4797
PagI TCATGA 2 cut(s) 1456, 6171
PceI AGGCCT 1 cut(s) 1831
PciI ACATGT 2 cut(s) 5345, 6331
PciSI GCTCTTC 3 cut(s) 1371, 1998, 5883
PcsI WCGNNNNNNNCGW 1 cut(s) 4867
PctI GAATGC 6 cut(s) 543, 1510, 2356, 3856, 4234, 4577
PdiI GCCGGC 1 cut(s) 980
PdmI GAANNNNTTC 9 cut(s) 345, 422, 543, 1350, 2723, 2789, 3161, 4521, 6387
PfoI TCCNGGA 1 cut(s) 2727
PinAI ACCGGT 1 cut(s) 5163
PmaCI CACGTG 1 cut(s) 5180
PmlI CACGTG 1 cut(s) 5180
Ppu21I YACGTR 2 cut(s) 5180, 5261
PpuMI RGGWCCY 1 cut(s) 3535
PscI ACATGT 2 cut(s) 5345, 6331
PshBI ATTAAT 4 cut(s) 1524, 2663, 4296, 5700
PsiI TTATAA 4 cut(s) 4100, 4508, 5822, 5850
Psp1406I AACGTT 3 cut(s) 3185, 5513, 6319
Psp5II RGGWCCY 1 cut(s) 3535
Psp6I CCWGG 4 cut(s) 2575, 2727, 2861, 3423
PspCI CACGTG 1 cut(s) 5180
PspEI GGTNACC 1 cut(s) 4543
PspFI CCCAGC 2 cut(s) 5296, 6983
PspGI CCWGG 4 cut(s) 2575, 2727, 2861, 3423
PspN4I GGNNCC 8 cut(s) 160, 342, 742, 1209, 2726, 3523, 3536, 6956
PspPI GGNCC 6 cut(s) 833, 1661, 3535, 5798, 6295, 7206
PspPPI RGGWCCY 1 cut(s) 3535
PstI CTGCAG 5 cut(s) 516, 2718, 5565, 5998, 6953
PstNI CAGNNNCTG 1 cut(s) 5026
PsuI RGATCY 9 cut(s) 158, 376, 2897, 3461, 3823, 4018, 5279, 5659, 5767
PvuII CAGCTG 5 cut(s) 14, 3022, 3551, 5560, 5624
RseI CAYNNNNRTG 4 cut(s) 503, 2239, 2930, 6928
SapI GCTCTTC 3 cut(s) 1371, 1998, 5883
Sau96I GGNCC 6 cut(s) 833, 1661, 3535, 5798, 6295, 7206
SbfI CCTGCAGG 1 cut(s) 6953
ScaI AGTACT 2 cut(s) 720, 5234
ScrFI CCNGG 6 cut(s) 769, 1799, 2577, 2729, 2863, 3425
SdaI CCTGCAGG 1 cut(s) 6953
SduI GDGCHC 2 cut(s) 1210, 5177
SfcI CTRYAG 9 cut(s) 118, 512, 1104, 2480, 2714, 5130, 5561, 5994, 6949
SfuI TTCGAA 1 cut(s) 2067
SinI GGWCC 4 cut(s) 3535, 5798, 6295, 7206
SmiI ATTTAAAT 1 cut(s) 2391
SmiMI CAYNNNNRTG 4 cut(s) 503, 2239, 2930, 6928
SmlI CTYRAG 6 cut(s) 828, 4166, 5078, 6032, 6161, 6631
SmoI CTYRAG 6 cut(s) 828, 4166, 5078, 6032, 6161, 6631
SpeI ACTAGT 1 cut(s) 2093
SphI GCATGC 2 cut(s) 2381, 4797
Sse8387I CCTGCAGG 1 cut(s) 6953
SseBI AGGCCT 1 cut(s) 1831
SspI AATATT 4 cut(s) 1498, 1905, 3161, 7135
StuI AGGCCT 1 cut(s) 1831
StyD4I CCNGG 6 cut(s) 767, 1797, 2575, 2727, 2861, 3423
StyI CCWWGG 5 cut(s) 2675, 5404, 5524, 5653, 6531
SwaI ATTTAAAT 1 cut(s) 2391
TaqI TCGA 8 cut(s) 310, 537, 2067, 3129, 4185, 4755, 5874, 5900
TaqII GACCGA 1 cut(s) 4723
TatI WGTACW 7 cut(s) 718, 839, 2638, 4441, 5232, 6600, 7497
TauI GCSGC 6 cut(s) 1068, 4565, 5569, 6567, 6644, 6647
TseFI GTSAC 8 cut(s) 427, 1400, 2987, 3367, 3712, 4543, 5548, 7531
Tsp45I GTSAC 8 cut(s) 427, 1400, 2987, 3367, 3712, 4543, 5548, 7531
TspGWI ACGGA 9 cut(s) 1931, 2975, 3549, 3800, 3816, 4019, 5485, 6786, 7482
Vha464I CTTAAG 3 cut(s) 5078, 6032, 6631
VpaK11BI GGWCC 4 cut(s) 3535, 5798, 6295, 7206
VspI ATTAAT 4 cut(s) 1524, 2663, 4296, 5700
XagI CCTNNNNNAGG 2 cut(s) 153, 5408
XbaI TCTAGA 4 cut(s) 1741, 2764, 3271, 7781
XceI RCATGY 7 cut(s) 2381, 2385, 4797, 5294, 5349, 6335, 6400
XcmI CCANNNNNNNNNTGG 3 cut(s) 1671, 4240, 5197
XmaJI CCTAGG 1 cut(s) 2675
XmiI GTMKAC 3 cut(s) 1647, 7280, 7617
XmnI GAANNNNTTC 9 cut(s) 345, 422, 543, 1350, 2723, 2789, 3161, 4521, 6387
ZraI GACGTC 1 cut(s) 1408
ZrmI AGTACT 2 cut(s) 720, 5234
Zsp2I ATGCAT 3 cut(s) 2383, 4154, 4795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.