AT4G31470

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011655
Physical Location & Seq
Reverse (-)
15261444 .. 15262412
969 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31470.1

Sequence Viewer

Length: 558 bp
ATGGAACAAAAAACTCTGATATTCATCATCATTATCATAGTTATCAGCACTCCACTTCCTTCTCTCTCTTTTCAAATCCCATCAAACAGAACACCGACAACATCAACACTAATTTTTTCTCAAGACAAAGCTCTAGCCCGCAACACCATTCAGCAGCAGTTTCTCCGACCACACAACATCTTACGAGCCAAGCTTCGTCTCCCTCCTCTCAAATGGAGCAATAGCCTCGCCCTCTATGCGAGCCGGTGGGCCAGAACCCGCCGTGGAGATTGCAAACTTATCCACTCCGGGGGACCTTACGGGGAGAATCTCTTCTGGGGAAGCGGAAAAGGTTGGACTCCACGGGATGCGGTGGCTGCATGGGCCTCGGAGATGAAATACTATGACCGGAGGACTTCCCACTGCAAGGCGAATGGGGATTGTTTGCATTACACGCAACTTGTATGGAAGAAGAGTTCGAGGATTGGGTGTGCAATCAGTTTTTGCAAGACCGGTGACACATTTATTATTTGTAACTATGATCCTCCTGGTAACATTGTTGGACAACCACCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

185

Amino Acids

20.93

Weight (kDa)

9.9

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 55 - 173 4e-24 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 139, 259, 324, 350
AclWI GGATC 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 289, 406
AgeI ACCGGT 1 cut(s) 491
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 526
AloI GAACNNNNNNTCC 2 cut(s) 439, 471
AluBI AGCT 2 cut(s) 131, 193
AluI AGCT 2 cut(s) 131, 193
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 515
AoxI GGCC 2 cut(s) 249, 363
ApeKI GCWGC 2 cut(s) 154, 356
AsiGI ACCGGT 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 249, 293, 363
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 1 cut(s) 293
BbvI GCAGC 2 cut(s) 166, 343
BccI CCATC 1 cut(s) 88
BceAI ACGGC 1 cut(s) 246
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 348, 382
BciT130I CCWGG 1 cut(s) 528
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 155, 357
BlsI GCNGC 2 cut(s) 156, 358
Bme1390I CCNGG 2 cut(s) 289, 528
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 3 cut(s) 249, 293, 363
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 2 cut(s) 289, 528
BmsI GCATC 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 289
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 262, 288, 341, 366
BsaWI WCCGGW 2 cut(s) 387, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 406
Bse118I RCCGGY 2 cut(s) 243, 491
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 4 cut(s) 262, 288, 341, 366
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 289, 406
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 166, 343
BshFI GGCC 2 cut(s) 251, 365
BshTI ACCGGT 1 cut(s) 491
BsiSI CCGG 4 cut(s) 244, 288, 388, 492
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 289, 406
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 2 cut(s) 251, 365
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 4 cut(s) 139, 259, 324, 350
BspANI GGCC 2 cut(s) 251, 365
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 1 cut(s) 515
BsrFI RCCGGY 2 cut(s) 243, 491
BssAI RCCGGY 2 cut(s) 243, 491
BssECI CCNNGG 4 cut(s) 262, 288, 341, 366
BssMI GATC 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 528
Bst6I CTCTTC 2 cut(s) 317, 446
BstC8I GCNNGC 2 cut(s) 139, 241
BstDSI CCRYGG 2 cut(s) 262, 341
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 4 cut(s) 236, 356, 362, 433
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 2 cut(s) 287, 526
BstV1I GCAGC 2 cut(s) 166, 343
BsuRI GGCC 2 cut(s) 251, 365
BtgI CCRYGG 2 cut(s) 262, 341
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 139, 241
Cfr10I RCCGGY 2 cut(s) 243, 491
Cfr13I GGNCC 3 cut(s) 249, 293, 363
CspAI ACCGGT 1 cut(s) 491
CviAII CATG 1 cut(s) 360
CviJI RGCY 9 cut(s) 131, 137, 188, 193, 225, 243, 251, 356, 365
CviKI_1 RGCY 9 cut(s) 131, 137, 188, 193, 225, 243, 251, 356, 365
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 2 cut(s) 317, 446
EarI CTCTTC 2 cut(s) 317, 446
Eco47I GGWCC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 526
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 1 cut(s) 363
FaiI YATR 6 cut(s) 38, 237, 361, 384, 445, 519
FaqI GGGAC 1 cut(s) 306
FatI CATG 1 cut(s) 359
FauI CCCGC 2 cut(s) 146, 266
Fnu4HI GCNGC 2 cut(s) 155, 357
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 2 cut(s) 155, 357
FspBI CTAG 1 cut(s) 134
GluI GCNGC 2 cut(s) 155, 357
HaeIII GGCC 2 cut(s) 251, 365
HapII CCGG 4 cut(s) 244, 288, 388, 492
Hin1II CATG 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 307, 337
HpaII CCGG 4 cut(s) 244, 288, 388, 492
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 3 cut(s) 18, 167, 370
Hpy188III TCNNGA 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 69
HpyCH4V TGCA 6 cut(s) 273, 359, 405, 427, 473, 486
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 356, 362, 433
Hsp92II CATG 1 cut(s) 363
Kzo9I GATC 1 cut(s) 520
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 8 cut(s) 257, 265, 301, 301, 401, 505, 513, 540
Lsp1109I GCAGC 2 cut(s) 166, 343
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 134
MaeIII GTNAC 3 cut(s) 494, 512, 530
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 3 cut(s) 304, 460, 463
MluCI AATT 1 cut(s) 111
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 3 cut(s) 190, 314, 520
MnlI CCTC 8 cut(s) 213, 216, 236, 242, 376, 384, 453, 534
MroXI GAANNNNTTC 1 cut(s) 311
MspI CCGG 4 cut(s) 244, 288, 388, 492
MspR9I CCNGG 2 cut(s) 289, 528
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 4 cut(s) 236, 356, 362, 433
NciI CCSGG 1 cut(s) 289
NdeII GATC 1 cut(s) 520
NlaIII CATG 1 cut(s) 363
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 1 cut(s) 307
PinAI ACCGGT 1 cut(s) 491
PkrI GCNGC 2 cut(s) 156, 358
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 3 cut(s) 249, 293, 363
PspPPI RGGWCCY 1 cut(s) 293
SatI GCNGC 2 cut(s) 155, 357
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 3 cut(s) 249, 293, 363
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 2 cut(s) 289, 528
SetI ASST 4 cut(s) 133, 195, 298, 334
SfaNI GCATC 1 cut(s) 337
SinI GGWCC 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 1 cut(s) 111
SsiI CCGC 4 cut(s) 139, 259, 324, 350
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 287, 526
TaqI TCGA 1 cut(s) 458
TasI AATT 1 cut(s) 111
TfiI GAWTC 1 cut(s) 307
TscAI CASTG 1 cut(s) 407
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 2 cut(s) 154, 356
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 2 cut(s) 13, 389
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.