MD10G1239100.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011655
Physical Location & Seq
Reverse (-)
33530812 .. 33531528
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1239100.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAAAGATAACATGTATAATTTCTATGAATTTGTTGAACGTACCTCCTCATTTCCACACTGTTTTGGCCCGCCATCTCTTTCCAAGCCTCATGAACTACAGTCCTCCACTCCCTACAACACCGTTCAACAATTTTTGATCCCTCACAACACTCAGAGATCAAGGCTCGGTCTCCGCCCTCTCAAATGGAACCGAAAACTGGAGAAATACGCTTCGCGGTGGGCACACCAGAGGCAAGAAGACTGCTCCTTGATTCATTCGGACAGCAAATTTGGCGAGAACCTCTTCTGGGGCAGCGGTCGAGATTGGAAGCCGGGTGACGCAGTTGCAGCATGGGCGCAAGAGAGAAGTTACTATAATTATTGGACGAATTCCTGTGCTCAGAACAAAGATTGCTGGCATTATACTCAGATGGTTTGGAGGCGGAGCTTGAAGATTGGTTGCTCCAAAGTTACATGTAGGGGTGGAGATACATTCATGGTTTGTGTCTATGACCCCCCGGGTAACTTTATAGGCCAAAAACCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

177

Amino Acids

20.74

Weight (kDa)

9.18

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 49 - 164 9e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 217
AciI CCGC 5 cut(s) 71, 175, 217, 297, 424
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 3 cut(s) 29, 269, 370
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 2 cut(s) 199, 289
AflIII ACRYGT 2 cut(s) 12, 455
AgsI TTSAA 3 cut(s) 38, 128, 433
AluBI AGCT 1 cut(s) 429
AluI AGCT 1 cut(s) 429
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 2 cut(s) 67, 514
ApeKI GCWGC 2 cut(s) 294, 329
ApoI RAATTY 3 cut(s) 29, 269, 370
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 3 cut(s) 315, 500, 501
AsuHPI GGTGA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 499
BaeGI GKGCMC 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 306, 341
BccI CCATC 2 cut(s) 82, 406
BcnI CCSGG 3 cut(s) 315, 500, 501
BcoDI GTCTC 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 98
BisI GCNGC 2 cut(s) 295, 330
BlsI GCNGC 2 cut(s) 296, 331
Bme1390I CCNGG 3 cut(s) 315, 500, 501
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 191
BmrFI CCNGG 3 cut(s) 315, 500, 501
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 221
BpuMI CCSGG 3 cut(s) 315, 500, 501
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 498, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 289
Bse1I ACTGG 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 498, 499
BseLI CCNNNNNNNGG 2 cut(s) 199, 289
BseMII CTCAG 3 cut(s) 167, 395, 422
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 2 cut(s) 306, 341
Bsh1236I CGCG 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 69, 516
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 2 cut(s) 314, 500
BslI CCNNNNNNNGG 2 cut(s) 199, 289
BsmAI GTCTC 1 cut(s) 176
BsnI GGCC 2 cut(s) 69, 516
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 226, 382
Bsp143I GATC 2 cut(s) 138, 158
BspACI CCGC 5 cut(s) 71, 175, 217, 297, 424
BspANI GGCC 2 cut(s) 69, 516
BspCNI CTCAG 3 cut(s) 166, 394, 421
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 191
BspPI GGATC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 498, 499
BssMI GATC 2 cut(s) 138, 158
Bst4CI ACNGT 3 cut(s) 62, 102, 124
Bst6I CTCTTC 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 71, 398
BstDEI CTNAG 3 cut(s) 153, 381, 408
BstFNI CGCG 1 cut(s) 217
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 2 cut(s) 141, 161
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 138, 158
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 3 cut(s) 273, 329, 335
BstNSI RCATGY 2 cut(s) 16, 459
BstSCI CCNGG 3 cut(s) 313, 498, 499
BstSFI CTRYAG 1 cut(s) 98
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 306, 341
BstV2I GAAGAC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 69, 516
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 71, 398
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 68
Cfr9I CCCGGG 1 cut(s) 499
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 42
CviAII CATG 5 cut(s) 13, 92, 333, 456, 478
CviJI RGCY 6 cut(s) 69, 88, 166, 313, 429, 516
CviKI_1 RGCY 6 cut(s) 69, 88, 166, 313, 429, 516
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 3 cut(s) 153, 381, 408
DpnI GATC 2 cut(s) 140, 160
DpnII GATC 2 cut(s) 138, 158
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
EciI GGCGGA 2 cut(s) 164, 439
Eco31I GGTCTC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 370
FaeI CATG 5 cut(s) 16, 95, 336, 459, 481
FatI CATG 5 cut(s) 12, 91, 332, 455, 477
FauI CCCGC 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 295, 330
Fsp4HI GCNGC 2 cut(s) 295, 330
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 295, 330
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 2 cut(s) 69, 516
HapII CCGG 2 cut(s) 314, 500
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 340
Hin1II CATG 5 cut(s) 16, 95, 336, 459, 481
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 1 cut(s) 253
HpaII CCGG 2 cut(s) 314, 500
HphI GGTGA 1 cut(s) 329
Hpy188I TCNGA 4 cut(s) 156, 262, 384, 411
Hpy188III TCNNGA 2 cut(s) 92, 302
HpyCH4III ACNGT 3 cut(s) 62, 102, 124
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 329, 335
HpyF3I CTNAG 3 cut(s) 153, 381, 408
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 5 cut(s) 16, 95, 336, 459, 481
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 2 cut(s) 138, 158
LmnI GCTCC 3 cut(s) 251, 426, 449
LpnPI CCDG 7 cut(s) 185, 242, 274, 327, 382, 388, 513
Lsp1109I GCAGC 2 cut(s) 306, 341
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 4 cut(s) 317, 350, 451, 503
MalI GATC 2 cut(s) 140, 160
MboI GATC 2 cut(s) 138, 158
MboII GAAGA 3 cut(s) 251, 277, 445
MhlI GDGCHC 2 cut(s) 226, 382
MluCI AATT 6 cut(s) 19, 29, 131, 269, 358, 370
MnlI CCTC 9 cut(s) 55, 58, 99, 115, 153, 189, 225, 293, 414
MroXI GAANNNNTTC 1 cut(s) 284
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 2 cut(s) 314, 500
MspR9I CCNGG 3 cut(s) 315, 500, 501
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 273, 329, 335
NciI CCSGG 3 cut(s) 315, 500, 501
NdeII GATC 2 cut(s) 138, 158
NlaIII CATG 5 cut(s) 16, 95, 336, 459, 481
NlaIV GGNNCC 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 2 cut(s) 16, 459
PagI TCATGA 1 cut(s) 91
PciI ACATGT 2 cut(s) 12, 455
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 2 cut(s) 296, 331
PscI ACATGT 2 cut(s) 12, 455
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 1 cut(s) 68
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
SatI GCNGC 2 cut(s) 295, 330
Sau3AI GATC 2 cut(s) 138, 158
Sau96I GGNCC 1 cut(s) 68
ScrFI CCNGG 3 cut(s) 315, 500, 501
SduI GDGCHC 2 cut(s) 226, 382
SetI ASST 4 cut(s) 43, 47, 285, 431
SfcI CTRYAG 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 501
Sse9I AATT 6 cut(s) 19, 29, 131, 269, 358, 370
SsiI CCGC 5 cut(s) 71, 175, 217, 297, 424
StyD4I CCNGG 3 cut(s) 313, 498, 499
TaaI ACNGT 3 cut(s) 62, 102, 124
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 301
TaqII GACCGA 1 cut(s) 158
TasI AATT 6 cut(s) 19, 29, 131, 269, 358, 370
TfiI GAWTC 1 cut(s) 253
TscAI CASTG 1 cut(s) 65
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 294, 329
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 5 cut(s) 17, 42, 108, 245, 466
TspMI CCCGGG 1 cut(s) 499
TspRI CASTG 1 cut(s) 65
XapI RAATTY 3 cut(s) 29, 269, 370
XceI RCATGY 2 cut(s) 16, 459
XmaI CCCGGG 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.