AT5G57625

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011655
Physical Location & Seq
Forward (+)
23337832 .. 23338855
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57625.1

Sequence Viewer

Length: 624 bp
ATGGCGACCACTTCATCGACAAAACAAGTCATTGTCTTCACAATTTCCCTTCTCTTGGTTGCTTTCCAAGCAGTCCATGCAGACTATTACCGTCAGAGGCCACCGGTTACCCCTACCCCTTACGTTCCTAAGCCACGGTACCCACTTCCGAGCCCGAGCCCCAAGCCTGTTTATCGACCACCTACTACCCCTAGTCTACCCGCTGGCTCAATCGCTAGACTATTCCTTGATCCACATAACGCTCTTCGGTCCGGACTAGGTTTGCCTCCATTGATTTGGGACGGTAAACTAGCGAGCTACGCAACATGGTGGGCTAACCAGAGGCGTTACGACTGCTCCTTAACCCATTCCACCGGCCCATACGGTGAGAACTTGTTCTGGGGAAGCGGCTCGAGCTGGGCACCAGGTTTCGCGGTGCAGTCATGGATCGTTGAAGGAAGGTCATACAACCACAACACCAACTCGTGTGACGGAAGTGGAATGTGTGGTCACTACACTCAGATGGTGTGGCGGGACACCAAAAGGCTCGGATGCGCACGTGTGGTGTGTGAGAACGGTGCCGGAGTTTTCATCACTTGTAACTACGACCCACCGGGTAACTACGTTGGCGAGAAGCCATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

207

Amino Acids

22.89

Weight (kDa)

9.17

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 76 - 195 2.4e-22 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 535
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 3 cut(s) 138, 400, 557
AccI GTMKAC 1 cut(s) 196
AccII CGCG 1 cut(s) 413
AccIII TCCGGA 1 cut(s) 251
AciI CCGC 4 cut(s) 201, 387, 413, 511
AclWI GGATC 2 cut(s) 224, 434
AcvI CACGTG 1 cut(s) 539
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 55
AflIII ACRYGT 1 cut(s) 538
AgeI ACCGGT 1 cut(s) 103
AgsI TTSAA 1 cut(s) 434
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 2 cut(s) 297, 396
AluI AGCT 2 cut(s) 297, 396
AlwI GGATC 2 cut(s) 224, 434
Ama87I CYCGRG 2 cut(s) 154, 391
Aor13HI TCCGGA 1 cut(s) 251
AoxI GGCC 2 cut(s) 98, 355
AsiGI ACCGGT 1 cut(s) 103
Asp700I GAANNNNTTC 1 cut(s) 374
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 2 cut(s) 249, 356
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 1 cut(s) 377
AvaI CYCGRG 2 cut(s) 154, 391
AvaII GGWCC 1 cut(s) 249
BaeGI GKGCMC 1 cut(s) 403
BanI GGYRCC 3 cut(s) 138, 400, 557
BanII GRGCYC 2 cut(s) 155, 161
BauI CACGAG 1 cut(s) 463
BbrPI CACGTG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 28
BccI CCATC 1 cut(s) 496
BciT130I CCWGG 1 cut(s) 405
BcnI CCSGG 1 cut(s) 594
BfaI CTAG 4 cut(s) 192, 216, 257, 290
BisI GCNGC 1 cut(s) 388
BlsI GCNGC 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 405, 594
Bme18I GGWCC 1 cut(s) 249
BmeT110I CYCGRG 2 cut(s) 154, 391
BmgT120I GGNCC 2 cut(s) 249, 356
BmiI GGNNCC 3 cut(s) 140, 402, 559
BmrFI CCNGG 2 cut(s) 405, 594
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 2 cut(s) 103, 251
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 2 cut(s) 103, 353
BseAI TCCGGA 1 cut(s) 251
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 1 cut(s) 536
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 396
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 2 cut(s) 100, 357
BshNI GGYRCC 3 cut(s) 138, 400, 557
BshTI ACCGGT 1 cut(s) 103
BsiHKCI CYCGRG 2 cut(s) 154, 391
BsiSI CCGG 5 cut(s) 104, 252, 354, 561, 593
BslFI GGGAC 2 cut(s) 293, 527
BslI CCNNNNNNNGG 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 293, 527
BsnI GGCC 2 cut(s) 100, 357
BsoBI CYCGRG 2 cut(s) 154, 391
Bsp1286I GDGCHC 3 cut(s) 155, 161, 403
Bsp13I TCCGGA 1 cut(s) 251
Bsp143I GATC 2 cut(s) 229, 426
BspACI CCGC 4 cut(s) 201, 387, 413, 511
BspANI GGCC 2 cut(s) 100, 357
BspCNI CTCAG 1 cut(s) 511
BspEI TCCGGA 1 cut(s) 251
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 3 cut(s) 140, 402, 559
BspPI GGATC 2 cut(s) 224, 434
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 3 cut(s) 138, 400, 557
BsrFI RCCGGY 2 cut(s) 103, 353
BssAI RCCGGY 2 cut(s) 103, 353
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 2 cut(s) 229, 426
BssSI CACGAG 1 cut(s) 463
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 5 cut(s) 92, 138, 284, 365, 557
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 205, 295
BstDEI CTNAG 2 cut(s) 129, 498
BstDSI CCRYGG 1 cut(s) 134
BstEII GGTNACC 1 cut(s) 106
BstF5I GGATG 1 cut(s) 536
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 2 cut(s) 232, 429
BstMBI GATC 2 cut(s) 229, 426
BstMWI GCNNNNNNNGC 4 cut(s) 68, 77, 299, 393
BstNI CCWGG 1 cut(s) 405
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 403, 592
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 413
BstV2I GAAGAC 1 cut(s) 28
BstXI CCANNNNNNTGG 1 cut(s) 276
BsuRI GGCC 2 cut(s) 100, 357
BtgI CCRYGG 1 cut(s) 134
BtsCI GGATG 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 205, 295
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 2 cut(s) 103, 353
Cfr13I GGNCC 2 cut(s) 249, 356
CpoI CGGWCCG 1 cut(s) 249
CsiI ACCWGGT 1 cut(s) 403
Csp6I GTAC 1 cut(s) 139
CspAI ACCGGT 1 cut(s) 103
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 3 cut(s) 77, 306, 423
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 129, 498
DpnI GATC 2 cut(s) 231, 428
DpnII GATC 2 cut(s) 229, 426
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 155, 161
Eco47I GGWCC 1 cut(s) 249
Eco72I CACGTG 1 cut(s) 539
Eco88I CYCGRG 2 cut(s) 154, 391
Eco91I GGTNACC 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 155, 161
FaeI CATG 3 cut(s) 80, 309, 426
FaiI YATR 7 cut(s) 78, 237, 307, 361, 424, 445, 619
FaqI GGGAC 2 cut(s) 293, 527
FatI CATG 3 cut(s) 76, 305, 422
FauI CCCGC 2 cut(s) 208, 504
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 388
FokI GGATG 1 cut(s) 543
FriOI GRGCYC 2 cut(s) 155, 161
Fsp4HI GCNGC 1 cut(s) 388
FspAI RTGCGCAY 1 cut(s) 535
FspBI CTAG 4 cut(s) 192, 216, 257, 290
FspI TGCGCA 1 cut(s) 535
GlaI GCGC 1 cut(s) 535
GluI GCNGC 1 cut(s) 388
GsaI CCCAGC 1 cut(s) 400
HaeIII GGCC 2 cut(s) 100, 357
HapII CCGG 5 cut(s) 104, 252, 354, 561, 593
HhaI GCGC 1 cut(s) 536
Hin1II CATG 3 cut(s) 80, 309, 426
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HpaII CCGG 5 cut(s) 104, 252, 354, 561, 593
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 2 cut(s) 197, 287
Hpy188I TCNGA 4 cut(s) 96, 150, 501, 530
Hpy188III TCNNGA 1 cut(s) 252
Hpy8I GTNNAC 2 cut(s) 197, 287
HpyAV CCTTC 3 cut(s) 59, 428, 432
HpyCH4III ACNGT 5 cut(s) 92, 138, 284, 365, 557
HpyCH4IV ACGT 3 cut(s) 123, 538, 603
HpyCH4V TGCA 2 cut(s) 80, 418
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 77, 299, 393
HpyF3I CTNAG 2 cut(s) 129, 498
HpySE526I ACGT 3 cut(s) 123, 538, 603
Hsp92II CATG 3 cut(s) 80, 309, 426
HspAI GCGC 1 cut(s) 534
Kpn2I TCCGGA 1 cut(s) 251
KpnI GGTACC 1 cut(s) 142
Kzo9I GATC 2 cut(s) 229, 426
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 341
LweI GCATC 1 cut(s) 521
MabI ACCWGGT 1 cut(s) 403
MaeI CTAG 4 cut(s) 192, 216, 257, 290
MaeII ACGT 3 cut(s) 123, 538, 603
MaeIII GTNAC 6 cut(s) 106, 326, 467, 488, 578, 596
MalI GATC 2 cut(s) 231, 428
MboI GATC 2 cut(s) 229, 426
MboII GAAGA 2 cut(s) 28, 236
MhlI GDGCHC 3 cut(s) 155, 161, 403
MluCI AATT 1 cut(s) 42
MnlI CCTC 3 cut(s) 90, 276, 315
MroI TCCGGA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 1 cut(s) 341
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 5 cut(s) 104, 252, 354, 561, 593
MspR9I CCNGG 2 cut(s) 405, 594
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 4 cut(s) 68, 77, 299, 393
NciI CCSGG 1 cut(s) 594
NdeII GATC 2 cut(s) 229, 426
NlaIII CATG 3 cut(s) 80, 309, 426
NlaIV GGNNCC 3 cut(s) 140, 402, 559
NmuCI GTSAC 2 cut(s) 467, 488
NsbI TGCGCA 1 cut(s) 535
PaeR7I CTCGAG 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 1 cut(s) 389
PmaCI CACGTG 1 cut(s) 539
PmlI CACGTG 1 cut(s) 539
Ppu21I YACGTR 1 cut(s) 539
Psp6I CCWGG 1 cut(s) 403
PspCI CACGTG 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 106
PspFI CCCAGC 1 cut(s) 396
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 3 cut(s) 140, 402, 559
PspPI GGNCC 2 cut(s) 249, 356
PspXI VCTCGAGB 1 cut(s) 391
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 500
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 1 cut(s) 388
Sau3AI GATC 2 cut(s) 229, 426
Sau96I GGNCC 2 cut(s) 249, 356
ScrFI CCNGG 2 cut(s) 405, 594
SduI GDGCHC 3 cut(s) 155, 161, 403
SetI ASST 9 cut(s) 126, 184, 262, 299, 398, 409, 443, 541, 606
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 1 cut(s) 521
Sfr274I CTCGAG 1 cut(s) 391
SinI GGWCC 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 1 cut(s) 42
SsiI CCGC 4 cut(s) 201, 387, 413, 511
SspMI CTAG 4 cut(s) 192, 216, 257, 290
StyD4I CCNGG 2 cut(s) 403, 592
TaaI ACNGT 5 cut(s) 92, 138, 284, 365, 557
TaiI ACGT 3 cut(s) 126, 541, 606
TaqI TCGA 3 cut(s) 17, 175, 392
TaqII GACCGA 1 cut(s) 237
TasI AATT 1 cut(s) 42
TauI GCSGC 1 cut(s) 390
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TseFI GTSAC 2 cut(s) 467, 488
Tsp45I GTSAC 2 cut(s) 467, 488
TspDTI ATGAA 1 cut(s) 559
TspGWI ACGGA 1 cut(s) 486
VpaK11BI GGWCC 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 4 cut(s) 192, 216, 257, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.