Rw5G012280

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011655
Physical Location & Seq
Forward (+)
14392355 .. 14392885
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012280.1

Sequence Viewer

Length: 531 bp
ATGGAAAACAAATCATTTTCAGTTGGGTTACTAAGAACGTTCCTCCTCCTTTCCACACTGTTTTGGCCATCTTTCTCACGCAGCTCACCCCATAAAACCTCCCCTACTCCCTCTTCTAACACCATTCAGCAGTACTTGATCCCTCACAACACTGAAAGATCAAAGCTCGGCCTCCCTCCTCTCAAATGGAGCAAGAAACTCGAGAGCTACGCTTCCTGGTGGGCCCGCCAACGGCGAGAAGACTGCTCGTTGATTCATTCGAACAGCGAGTTCGGGGAGAACCTCTTCTGGGGGAGTGGACATGACTGGAAGCCGAGTGATGCAGTTAAAGCATGGGCAACAGAGAGTTGCTACTACAACCACAAGACTAACTCTTGTAATCAGAAAAAGGATTGCTTGCATTATACTCAAATGGTTTGGAAGCAGAGCTCGAAGATTGGTTGTGCTAAAGTTATATGTAAGAGTGGGGATACAATTTTTGCTTGTGTTTACGATCCTCCAGGTAATGTTATAGGCCAAAAGCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

176

Amino Acids

20.13

Weight (kDa)

9.27

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 49 - 164 9.9e-21 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 226
AclI AACGTT 1 cut(s) 38
AclWI GGATC 2 cut(s) 133, 488
AcoI YGGCCR 1 cut(s) 65
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 231, 289
AjnI CCWGG 2 cut(s) 215, 499
AluBI AGCT 4 cut(s) 84, 166, 207, 429
AluI AGCT 4 cut(s) 84, 166, 207, 429
Alw21I GWGCWC 1 cut(s) 431
AlwI GGATC 2 cut(s) 133, 488
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 4 cut(s) 65, 169, 222, 514
ApaI GGGCCC 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 81
Asp700I GAANNNNTTC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 222, 223
AsuHPI GGTGA 1 cut(s) 78
AsuII TTCGAA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 67
BanII GRGCYC 2 cut(s) 226, 431
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 1 cut(s) 93
BccI CCATC 1 cut(s) 76
BceAI ACGGC 1 cut(s) 248
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 225, 259
BciT130I CCWGG 2 cut(s) 217, 501
BciVI GTATCC 1 cut(s) 463
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 217, 501
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 2 cut(s) 222, 223
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 2 cut(s) 217, 501
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 260
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 289
Bse1I ACTGG 1 cut(s) 311
BseBI CCWGG 2 cut(s) 217, 501
BseLI CCNNNNNNNGG 2 cut(s) 231, 289
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 2 cut(s) 35, 168
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 1 cut(s) 93
BshFI GGCC 4 cut(s) 67, 171, 224, 516
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 231, 289
BsnI GGCC 4 cut(s) 67, 171, 224, 516
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 222
Bsp1286I GDGCHC 2 cut(s) 226, 431
Bsp143I GATC 3 cut(s) 138, 158, 493
BspACI CCGC 1 cut(s) 226
BspANI GGCC 4 cut(s) 67, 171, 224, 516
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 2 cut(s) 133, 488
BspT104I TTCGAA 1 cut(s) 260
BsrI ACTGG 1 cut(s) 311
BssMI GATC 3 cut(s) 138, 158, 493
Bst2UI CCWGG 2 cut(s) 217, 501
Bst4CI ACNGT 1 cut(s) 60
Bst6I CTCTTC 2 cut(s) 118, 290
BstBI TTCGAA 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 226, 398
BstDEI CTNAG 1 cut(s) 32
BstKTI GATC 3 cut(s) 141, 161, 496
BstMBI GATC 3 cut(s) 138, 158, 493
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 2 cut(s) 217, 501
BstSCI CCNGG 2 cut(s) 215, 499
BstSLI GKGCMC 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 246
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 4 cut(s) 67, 171, 224, 516
BtsIMutI CAGTG 2 cut(s) 56, 150
Cac8I GCNNGC 2 cut(s) 226, 398
Cfr13I GGNCC 2 cut(s) 222, 223
Csp6I GTAC 1 cut(s) 133
CviAII CATG 2 cut(s) 302, 333
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 32
DpnI GATC 3 cut(s) 140, 160, 495
DpnII GATC 3 cut(s) 138, 158, 493
EaeI YGGCCR 1 cut(s) 65
Eam1104I CTCTTC 2 cut(s) 118, 290
EarI CTCTTC 2 cut(s) 118, 290
Ecl136II GAGCTC 1 cut(s) 429
Eco24I GRGCYC 2 cut(s) 226, 431
Eco53kI GAGCTC 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 429
EcoRII CCWGG 2 cut(s) 215, 499
EcoT38I GRGCYC 2 cut(s) 226, 431
FaeI CATG 2 cut(s) 305, 336
FaiI YATR 7 cut(s) 93, 303, 334, 405, 455, 457, 512
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 2 cut(s) 301, 332
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 82
FriOI GRGCYC 2 cut(s) 226, 431
Fsp4HI GCNGC 1 cut(s) 82
GluI GCNGC 1 cut(s) 82
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 4 cut(s) 67, 171, 224, 516
Hin1II CATG 2 cut(s) 305, 336
HinfI GANTC 1 cut(s) 253
HphI GGTGA 1 cut(s) 78
Hpy166II GTNNAC 2 cut(s) 299, 490
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 1 cut(s) 202
Hpy8I GTNNAC 2 cut(s) 299, 490
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 2 cut(s) 323, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 2 cut(s) 305, 336
Kzo9I GATC 3 cut(s) 138, 158, 493
LmnI GCTCC 1 cut(s) 189
LpnPI CCDG 6 cut(s) 202, 229, 274, 292, 486, 513
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 1 cut(s) 310
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 27
MalI GATC 3 cut(s) 140, 160, 495
MboI GATC 3 cut(s) 138, 158, 493
MboII GAAGA 4 cut(s) 105, 251, 277, 445
MhlI GDGCHC 2 cut(s) 226, 431
MlsI TGGCCA 1 cut(s) 67
MluCI AATT 1 cut(s) 474
MluNI TGGCCA 1 cut(s) 67
Mox20I TGGCCA 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 284
MscI TGGCCA 1 cut(s) 67
MseI TTAA 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 67
MspR9I CCNGG 2 cut(s) 217, 501
MvaI CCWGG 2 cut(s) 217, 501
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeII GATC 3 cut(s) 138, 158, 493
NlaIII CATG 2 cut(s) 305, 336
NlaIV GGNNCC 1 cut(s) 224
NmeAIII GCCGAG 2 cut(s) 147, 339
NspV TTCGAA 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 215, 499
PspGI CCWGG 2 cut(s) 215, 499
PspN4I GGNNCC 1 cut(s) 224
PspOMI GGGCCC 1 cut(s) 222
PspPI GGNCC 2 cut(s) 222, 223
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SacI GAGCTC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 3 cut(s) 138, 158, 493
Sau96I GGNCC 2 cut(s) 222, 223
ScaI AGTACT 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 217, 501
SduI GDGCHC 2 cut(s) 226, 431
SetI ASST 8 cut(s) 41, 86, 101, 168, 209, 285, 431, 505
SfaNI GCATC 1 cut(s) 310
Sfr274I CTCGAG 1 cut(s) 200
SfuI TTCGAA 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 1 cut(s) 474
SsiI CCGC 1 cut(s) 226
SstI GAGCTC 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 215, 499
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 1 cut(s) 41
TaqI TCGA 3 cut(s) 201, 260, 431
TasI AATT 1 cut(s) 474
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 63, 157
TseI GCWGC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 245
TspRI CASTG 2 cut(s) 63, 157
XhoI CTCGAG 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.