AT4G32130

ER membrane protein complex subunit 7 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013481
Physical Location & Seq
Reverse (-)
15520025 .. 15522695
2671 bp
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UTR
Exon/CDS
Intron
AT4G32130.1

Sequence Viewer

Length: 609 bp
ATGGCGCCGATTTTCAGATCCACTTCCCTTATCGCTTTTTCTCTCTTCTTCTTCTTCTTTGCTTCCACTCTTCCGATCTCATCTGGGTCTGAGGATAGTTACACGATCACGGGTAGAGTGAGGGTTCCAGCAAGCACCGTGATTGGTCATGCAGCTAAATTCTCAAATATCAAAGTTATACTCAACGGTGGACAGCATGTTACTTTCCTCAGGCCGGATGGATACTTTACCTTCCACAAAGTGCCAGCGGGGACTCATCTGATTGAAGTTTATGCACTGGGCTACTTCTTCTCCCCAGTGCGAGTTGATGTTAGTGCTCGGCATCGTGGCAAGGTTCAAGCAACACTTACAGAAACCAGAAGGAGTCTTACTGAGCTGGTTTTGGAGCCATTGCGGGCAGAGCAATACTACGAGATGCGAGAACCTTTCTCTGTAATGTCAATTGTGAAAAGTCCAATGGGTCTTATGGTGGGATTCATGGTCGTTGTCGTGTTCTTAATGCCTAAGTTGATGGAAAACATAGATCCCGAGGAAATGAAGAGCGCACAAGAGCAGATGAGAAGCCAAGGAGTGCCTTCACTCACTAGCTTGTTACCCGCTAGCCGCTAA

Protein Analysis

202

Amino Acids

22.56

Weight (kDa)

9.47

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC7_beta-sandw PF09430 52 - 156 2e-23 ER membrane protein complex subunit 7, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25310 AT4G32130
fragaria_vesca FvH4_2g28770
malus_domestica MD08G1157200.v1.1 MD15G1128300.v1.1
prunus_persica Prupe.1G483600_v2.0.a1
pyrus_communis pycom15g11550
rosa_chinensis RchiOBHm_Chr6g0297531
rosa_laevigata RLG00000011586
rosa_multiflora Rmu_sc0003740.1_g000001 Rmu_ssc0000319.1_g000002
rosa_roxburghii Rroxscaffold_7G00170390
rosa_rugosa Rorug06G0273300
rosa_samantha Rh6AG384300 Rh6BG392400 Rh6CG398300 Rh6DG384800
rosa_wichuraiana Rw6G033470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 4 cut(s) 248, 394, 597, 604
AclWI GGATC 2 cut(s) 12, 518
AcsI RAATTY 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 2 cut(s) 266, 338
AluBI AGCT 3 cut(s) 155, 376, 588
AluI AGCT 3 cut(s) 155, 376, 588
Alw21I GWGCWC 1 cut(s) 319
AlwI GGATC 2 cut(s) 12, 518
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 1 cut(s) 212
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 158
AspLEI GCGC 2 cut(s) 7, 545
AsuNHI GCTAGC 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 209
BanI GGYRCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 352, 384
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 164
BccI CCATC 2 cut(s) 212, 505
BciVI GTATCC 1 cut(s) 215
BfaI CTAG 2 cut(s) 585, 600
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 215
BisI GCNGC 2 cut(s) 153, 604
BlsI GCNGC 2 cut(s) 154, 605
BmeT110I CYCGRG 1 cut(s) 527
BmiI GGNNCC 3 cut(s) 6, 126, 387
BmrI ACTGGG 2 cut(s) 287, 290
BmsI GCATC 2 cut(s) 331, 405
BmtI GCTAGC 1 cut(s) 603
BmuI ACTGGG 2 cut(s) 287, 290
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 528, 565
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 282, 296
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 528, 565
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 389
BseMII CTCAG 3 cut(s) 81, 223, 363
BseNI ACTGG 2 cut(s) 282, 296
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 4 cut(s) 17, 75, 105, 523
BspACI CCGC 4 cut(s) 248, 394, 597, 604
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 3 cut(s) 82, 222, 364
BspLI GGNNCC 3 cut(s) 6, 126, 387
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 2 cut(s) 12, 518
BspQI GCTCTTC 1 cut(s) 533
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 389
BsrI ACTGG 2 cut(s) 282, 296
BssECI CCNNGG 2 cut(s) 528, 565
BssMI GATC 4 cut(s) 17, 75, 105, 523
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 565
Bst4CI ACNGT 2 cut(s) 139, 188
Bst6I CTCTTC 3 cut(s) 50, 75, 533
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 133, 246, 396, 601
BstDEI CTNAG 4 cut(s) 90, 209, 372, 504
BstF5I GGATG 1 cut(s) 223
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 545
BstKTI GATC 4 cut(s) 20, 78, 108, 526
BstMBI GATC 4 cut(s) 17, 75, 105, 523
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 164
BstX2I RGATCY 2 cut(s) 17, 523
BstYI RGATCY 2 cut(s) 17, 523
Bsu36I CCTNAGG 1 cut(s) 209
BsuI GTATCC 1 cut(s) 215
BsuRI GGCC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 275, 303
Cac8I GCNNGC 4 cut(s) 133, 246, 396, 601
CfoI GCGC 2 cut(s) 7, 545
CviAII CATG 3 cut(s) 149, 197, 478
CviJI RGCY 8 cut(s) 155, 214, 282, 376, 388, 564, 588, 603
CviKI_1 RGCY 8 cut(s) 155, 214, 282, 376, 388, 564, 588, 603
DdeI CTNAG 4 cut(s) 90, 209, 372, 504
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 19, 77, 107, 525
DpnII GATC 4 cut(s) 17, 75, 105, 523
DraIII CACNNNGTG 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 50, 75, 533
EarI CTCTTC 3 cut(s) 50, 75, 533
Eco130I CCWWGG 1 cut(s) 565
Eco81I CCTNAGG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 565
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 565
FaeI CATG 3 cut(s) 152, 200, 481
FaiI YATR 7 cut(s) 150, 179, 198, 273, 467, 479, 521
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 1 cut(s) 265
FatI CATG 3 cut(s) 148, 196, 477
FauI CCCGC 3 cut(s) 241, 387, 604
Fnu4HI GCNGC 2 cut(s) 153, 604
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 2 cut(s) 153, 604
FspBI CTAG 2 cut(s) 585, 600
GlaI GCGC 2 cut(s) 6, 544
GluI GCNGC 2 cut(s) 153, 604
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 214
HapII CCGG 1 cut(s) 215
HhaI GCGC 2 cut(s) 7, 545
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 152, 200, 481
Hin6I GCGC 2 cut(s) 5, 543
HinP1I GCGC 2 cut(s) 5, 543
HinfI GANTC 3 cut(s) 253, 364, 474
HpaII CCGG 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 4 cut(s) 17, 75, 91, 261
Hpy188III TCNNGA 1 cut(s) 527
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 241, 354, 585
HpyCH4III ACNGT 2 cut(s) 139, 188
HpyCH4V TGCA 2 cut(s) 152, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 4 cut(s) 90, 209, 372, 504
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 152, 200, 481
HspAI GCGC 2 cut(s) 5, 543
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 4 cut(s) 17, 75, 105, 523
LguI GCTCTTC 1 cut(s) 533
LmnI GCTCC 1 cut(s) 385
LpnPI CCDG 9 cut(s) 69, 141, 196, 228, 258, 263, 309, 362, 370
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 2 cut(s) 331, 405
MaeI CTAG 2 cut(s) 585, 600
MaeIII GTNAC 3 cut(s) 98, 199, 591
MalI GATC 4 cut(s) 19, 77, 107, 525
MboI GATC 4 cut(s) 17, 75, 105, 523
MboII GAAGA 7 cut(s) 37, 40, 43, 46, 62, 280, 550
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 2 cut(s) 17, 523
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 2 cut(s) 158, 441
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 247, 373
MnlI CCTC 4 cut(s) 85, 114, 218, 523
MseI TTAA 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 215
MunI CAATTG 1 cut(s) 441
MwoI GCNNNNNNNGC 1 cut(s) 400
NarI GGCGCC 1 cut(s) 5
NdeII GATC 4 cut(s) 17, 75, 105, 523
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 3 cut(s) 152, 200, 481
NlaIV GGNNCC 3 cut(s) 6, 126, 387
NmeAIII GCCGAG 1 cut(s) 298
NspI RCATGY 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 474
PkrI GCNGC 2 cut(s) 154, 605
PleI GAGTC 2 cut(s) 247, 372
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 247, 372
PspN4I GGNNCC 3 cut(s) 6, 126, 387
PsuI RGATCY 2 cut(s) 17, 523
SapI GCTCTTC 1 cut(s) 533
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 153, 604
Sau3AI GATC 4 cut(s) 17, 75, 105, 523
SchI GAGTC 2 cut(s) 247, 373
SduI GDGCHC 1 cut(s) 319
SetI ASST 6 cut(s) 157, 233, 336, 378, 427, 590
SfaNI GCATC 2 cut(s) 331, 405
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 2 cut(s) 158, 441
SsiI CCGC 4 cut(s) 248, 394, 597, 604
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 585, 600
StyI CCWWGG 1 cut(s) 565
TaaI ACNGT 2 cut(s) 139, 188
TasI AATT 2 cut(s) 158, 441
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 1 cut(s) 474
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 2 cut(s) 282, 303
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 466, 551
TspRI CASTG 2 cut(s) 282, 303
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 200
XspI CTAG 2 cut(s) 585, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.