RLG00000011586

ER membrane protein complex subunit 7 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013481
Physical Location & Seq
Forward (+)
10724635 .. 10726890
2256 bp
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UTR
Exon/CDS
Intron
RLM00000011586

Sequence Viewer

Length: 624 bp
ATGGCGTGGAGCTTCCGATCCAAACCGTTCCTTGTCGTGCTTCTCCTACAGTTGTGCTTCCTGCTCATCTCTCCGTCACTCGCAATTTCTCCAGAGTCTCCTGATGGTTACACCATTCACGGCCGAGTGAAGATCCCGAGTCTGGGGGTGAAAGGATTTGGTCTTCATGGAAAAATATCTAATATCAAAGTCATACTAAATGGTGGCCAAAGTGTTACCTATCTGAGGCCCGACGGGTTTTTCTCATTCCATAATGTACCTGCGGGGACTCATCTAGTTGAAGTGTCTGCAATAGGTTATTTCTTTTCTCCGGTCCGAGTTGATGTTAGTGCCAGAAACCCAGGCAAGGTTCAGGCAGCACTGACTGAGAATAGGAGGGGTCTACTTGAATTTGTTTTAGAGCCACTTAGGGAGGAACAGTATTATGAGATAAGGGAACCCTTCTCCATAATGTCTGTCGTAAAGAGCCCGATGGGTCTGATGGTTGGATTTATGGTGATTGTTGTTTTTGTGATGCCCAAACTAGTGGAGAACATGGATCCTGAAGAAATGAGGCGAACCCAAGAAGCAATGGGAAATGGAGGGCTGGCAAGCATGTTACCGGGTGCTGCTGCGAGGAATTAA

Protein Analysis

208

Amino Acids

22.78

Weight (kDa)

9.1

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC7_beta-sandw PF09430 59 - 161 9.6e-22 ER membrane protein complex subunit 7, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25310 AT4G32130
fragaria_vesca FvH4_2g28770
malus_domestica MD08G1157200.v1.1 MD15G1128300.v1.1
prunus_persica Prupe.1G483600_v2.0.a1
pyrus_communis pycom15g11550
rosa_chinensis RchiOBHm_Chr6g0297531
rosa_laevigata RLG00000011586
rosa_multiflora Rmu_sc0003740.1_g000001 Rmu_ssc0000319.1_g000002
rosa_roxburghii Rroxscaffold_7G00170390
rosa_rugosa Rorug06G0273300
rosa_samantha Rh6AG384300 Rh6BG392400 Rh6CG398300 Rh6DG384800
rosa_wichuraiana Rw6G033470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 382
AciI CCGC 1 cut(s) 263
AclWI GGATC 4 cut(s) 12, 127, 533, 546
AcoI YGGCCR 2 cut(s) 121, 205
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 4 cut(s) 142, 143, 225, 346
AgsI TTSAA 2 cut(s) 281, 389
AhlI ACTAGT 1 cut(s) 523
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 4 cut(s) 12, 127, 533, 546
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 3 cut(s) 121, 205, 227
ApeKI GCWGC 3 cut(s) 356, 608, 611
ApoI RAATTY 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 228, 313
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 2 cut(s) 160, 508
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 207
BamHI GGATCC 1 cut(s) 538
BanII GRGCYC 1 cut(s) 470
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 368, 595, 598
BccI CCATC 3 cut(s) 98, 466, 475
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 102
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 2 cut(s) 275, 524
BfmI CTRYAG 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 3 cut(s) 357, 609, 612
BlsI GCNGC 3 cut(s) 358, 610, 613
Bme1390I CCNGG 2 cut(s) 342, 603
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 228, 313
BmiI GGNNCC 2 cut(s) 438, 540
BmrFI CCNGG 2 cut(s) 342, 603
BmsI GCATC 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 340
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 143, 225, 346
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 340
BseLI CCNNNNNNNGG 4 cut(s) 142, 143, 225, 346
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 2 cut(s) 215, 357
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 3 cut(s) 368, 595, 598
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 123, 207, 229
BsiEI CGRYCG 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 311, 602
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 4 cut(s) 142, 143, 225, 346
BsmAI GTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 3 cut(s) 123, 207, 229
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 3 cut(s) 17, 132, 538
BspACI CCGC 1 cut(s) 263
BspANI GGCC 3 cut(s) 123, 207, 229
BspCNI CTCAG 2 cut(s) 216, 358
BspLI GGNNCC 2 cut(s) 438, 540
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 4 cut(s) 12, 127, 533, 546
BsrDI GCAATG 1 cut(s) 576
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 3 cut(s) 17, 132, 538
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 27, 51, 420
BstC8I GCNNGC 2 cut(s) 588, 592
BstDEI CTNAG 3 cut(s) 224, 366, 407
BstENI CCTNNNNNAGG 1 cut(s) 223
BstKTI GATC 3 cut(s) 20, 135, 541
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 3 cut(s) 17, 132, 538
BstMCI CGRYCG 1 cut(s) 124
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 340, 601
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 368, 595, 598
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 2 cut(s) 132, 538
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 2 cut(s) 132, 538
BstZI CGGCCG 1 cut(s) 121
BsuRI GGCC 3 cut(s) 123, 207, 229
BtsIMutI CAGTG 1 cut(s) 359
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 588, 592
Cfr13I GGNCC 2 cut(s) 228, 313
CpoI CGGWCCG 1 cut(s) 313
Csp6I GTAC 1 cut(s) 257
CspI CGGWCCG 1 cut(s) 313
CviAII CATG 3 cut(s) 167, 535, 595
CviJI RGCY 7 cut(s) 12, 123, 207, 229, 403, 468, 586
CviKI_1 RGCY 7 cut(s) 12, 123, 207, 229, 403, 468, 586
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 3 cut(s) 224, 366, 407
DpnI GATC 3 cut(s) 19, 134, 540
DpnII GATC 3 cut(s) 17, 132, 538
EaeI YGGCCR 2 cut(s) 121, 205
EagI CGGCCG 1 cut(s) 121
EclXI CGGCCG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 313
Eco52I CGGCCG 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 470
FaeI CATG 3 cut(s) 170, 538, 598
FaiI YATR 8 cut(s) 168, 194, 252, 426, 449, 494, 536, 596
FaqI GGGAC 1 cut(s) 280
FatI CATG 3 cut(s) 166, 534, 594
FauI CCCGC 1 cut(s) 256
FblI GTMKAC 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 357, 609, 612
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 3 cut(s) 357, 609, 612
FspBI CTAG 2 cut(s) 275, 524
GluI GCNGC 3 cut(s) 357, 609, 612
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 3 cut(s) 123, 207, 229
HapII CCGG 2 cut(s) 311, 602
Hin1II CATG 3 cut(s) 170, 538, 598
HinfI GANTC 3 cut(s) 95, 139, 268
HpaII CCGG 2 cut(s) 311, 602
HphI GGTGA 2 cut(s) 160, 508
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 4 cut(s) 17, 225, 317, 480
Hpy188III TCNNGA 4 cut(s) 92, 101, 136, 542
Hpy8I GTNNAC 1 cut(s) 383
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 27, 51, 420
HpyCH4V TGCA 1 cut(s) 290
HpyF3I CTNAG 3 cut(s) 224, 366, 407
Hsp92II CATG 3 cut(s) 170, 538, 598
Kzo9I GATC 3 cut(s) 17, 132, 538
LmnI GCTCC 1 cut(s) 9
Lsp1109I GCAGC 3 cut(s) 368, 595, 598
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 275, 524
MaeIII GTNAC 4 cut(s) 75, 107, 214, 597
MalI GATC 3 cut(s) 19, 134, 540
MboI GATC 3 cut(s) 17, 132, 538
MboII GAAGA 3 cut(s) 142, 155, 557
MflI RGATCY 2 cut(s) 132, 538
MhlI GDGCHC 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 3 cut(s) 84, 389, 619
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 3 cut(s) 104, 148, 262
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 6 cut(s) 219, 369, 406, 546, 575, 609
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 1 cut(s) 622
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 2 cut(s) 311, 602
MspR9I CCNGG 2 cut(s) 342, 603
MvaI CCWGG 1 cut(s) 342
NciI CCSGG 1 cut(s) 603
NdeII GATC 3 cut(s) 17, 132, 538
NlaIII CATG 3 cut(s) 170, 538, 598
NlaIV GGNNCC 2 cut(s) 438, 540
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 75
NspI RCATGY 1 cut(s) 598
PkrI GCNGC 3 cut(s) 358, 610, 613
PleI GAGTC 3 cut(s) 103, 147, 262
PpsI GAGTC 3 cut(s) 103, 147, 262
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 438, 540
PspPI GGNCC 2 cut(s) 228, 313
PsuI RGATCY 2 cut(s) 132, 538
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
Rsr2I CGGWCCG 1 cut(s) 313
RsrII CGGWCCG 1 cut(s) 313
SaqAI TTAA 1 cut(s) 622
SatI GCNGC 3 cut(s) 357, 609, 612
Sau3AI GATC 3 cut(s) 17, 132, 538
Sau96I GGNCC 2 cut(s) 228, 313
SchI GAGTC 3 cut(s) 104, 148, 262
ScrFI CCNGG 2 cut(s) 342, 603
SduI GDGCHC 1 cut(s) 470
SetI ASST 5 cut(s) 14, 221, 262, 298, 351
SfaNI GCATC 1 cut(s) 504
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 313
SpeI ACTAGT 1 cut(s) 523
Sse9I AATT 3 cut(s) 84, 389, 619
SsiI CCGC 1 cut(s) 263
SspMI CTAG 2 cut(s) 275, 524
StyD4I CCNGG 2 cut(s) 340, 601
TaaI ACNGT 3 cut(s) 27, 51, 420
TasI AATT 3 cut(s) 84, 389, 619
Tru1I TTAA 1 cut(s) 622
Tru9I TTAA 1 cut(s) 622
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 3 cut(s) 356, 608, 611
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 223
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 382
XspI CTAG 2 cut(s) 275, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.