pycom15g11550

ER membrane protein complex subunit 7 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013481
Physical Location & Seq
Forward (+)
7811331 .. 7814991
3661 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11550.1

Sequence Viewer

Length: 1059 bp
ATGGCAGTAAACAAGTACGGACCTGATTTCGCCGGCTACAACAATTTAGCAAATTCCGTAACACACCAGATCCTTCTCAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGACACAAACACTGTGAAACTCTCGCAAAAGCTCTCTCAGTGTTTCGTTATGGCGCGGAGCTGGAGATCCAATCTGGTGGTTCTCGTGCTTTTCCTACAGTTGTGCTTTTCGCTCATCTCTTCGTCGCTCGCAATTTCTCCCGGCTCTGCCGATGGATACACCATTCATGGCCGAGTAAAGATCCCACCCAGTCTCGGGGTGAAAGGGTTTGCACCTTACGGAAAGATATCAAATATCAAAGTCATACTAAATGGTGGTCAAAGTGTTACCTTTCTGAGGCCTGATGGATTTTTTTCATTCCATAATGTGCCTGCGGGGACTCATTTGATCGAAGTGTCTGCAATAGGCTATTTCTTCTCTCCGGTCCGAGTTGATGTTAGTGCTAGAAACCCAGGCAAGGTTCAGGCAGCACTGACTGAGAATAGGAGGCCACTGAATGAGTTTGTTTTGGAGCCATTGAGAGAGGAACAGTATTATGAGATAAGAGAACCCTTCTCCATAATGGCTCTTGTAAAGAGCCCCATGGGACTGATGGTTGGATTTATGCTAATTGTCGTGTTTCTTATGCCGAAATTAGTGGAGAACATGGATCCTGAAGAAATGAGGCGAACACAAGAAGAAATGAGAAACCAAGGGGTTCCATCGCTGGCAAGTTTTTTGGCAGACGTCCAGTATCGCATTAGCAAATGCACTAAATCTGCACCTCCAATATCTCGCCCAAATCATTGCTCTGGTCTTTTCGCGGTCTTCTCTCTGCTGATTCGGAATCGGAGATGGAGGGTTTGCCGACGACGACGAAATCAGATCGGCGATGGCGATCGAAGCCCCTGCAGACCTCCAAGCCATCCATTCTCATGGCATTCTTCTCCTGCGTCGCTTGGCTCTACGTCGCCGGCCGGTGTTGTGGCAGGATGCGGAGAACAGAACTGTGCTTTCTAA

Protein Analysis

353

Amino Acids

38.87

Weight (kDa)

9.6

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC7_beta-sandw PF09430 115 - 218 6e-22 ER membrane protein complex subunit 7, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25310 AT4G32130
fragaria_vesca FvH4_2g28770
malus_domestica MD08G1157200.v1.1 MD15G1128300.v1.1
prunus_persica Prupe.1G483600_v2.0.a1
pyrus_communis pycom15g11550
rosa_chinensis RchiOBHm_Chr6g0297531
rosa_laevigata RLG00000011586
rosa_multiflora Rmu_sc0003740.1_g000001 Rmu_ssc0000319.1_g000002
rosa_roxburghii Rroxscaffold_7G00170390
rosa_rugosa Rorug06G0273300
rosa_samantha Rh6AG384300 Rh6BG392400 Rh6CG398300 Rh6DG384800
rosa_wichuraiana Rw6G033470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 789
AccII CGCG 2 cut(s) 175, 863
AciI CCGC 4 cut(s) 175, 434, 863, 1035
AclWI GGATC 5 cut(s) 64, 180, 295, 704, 717
AcoI YGGCCR 2 cut(s) 289, 1014
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 1 cut(s) 786
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 4 cut(s) 314, 315, 396, 517
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 2 cut(s) 151, 180
AluI AGCT 2 cut(s) 151, 180
Alw26I GTCTC 1 cut(s) 317
AlwI GGATC 5 cut(s) 64, 180, 295, 704, 717
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 4 cut(s) 289, 398, 548, 1014
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 1 cut(s) 52
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 2 cut(s) 20, 484
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 331
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 20, 484
BamHI GGATCC 1 cut(s) 709
BanII GRGCYC 1 cut(s) 641
BauI CACGAG 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 859
BbvI GCAGC 1 cut(s) 539
BccI CCATC 7 cut(s) 266, 398, 646, 769, 888, 926, 972
BcgI CGANNNNNNTGC 4 cut(s) 440, 474, 930, 964
BciT130I CCWGG 1 cut(s) 513
BciVI GTATCC 1 cut(s) 269
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 317
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 2 cut(s) 215, 949
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 261, 513
Bme18I GGWCC 2 cut(s) 20, 484
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 20, 484
BmiI GGNNCC 3 cut(s) 573, 711, 759
BmrFI CCNGG 2 cut(s) 261, 513
BmrI ACTGGG 1 cut(s) 303
BmsI GCATC 1 cut(s) 1022
BmuI ACTGGG 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 859
BpmI CTGGAG 1 cut(s) 202
BpuMI CCSGG 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 1 cut(s) 786
BsaJI CCNNGG 3 cut(s) 511, 642, 751
BsaWI WCCGGW 1 cut(s) 481
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 315, 396, 517
Bse118I RCCGGY 3 cut(s) 32, 1012, 1016
Bse1I ACTGG 2 cut(s) 309, 790
Bse3DI GCAATG 1 cut(s) 844
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 511, 642, 751
BseGI GGATG 2 cut(s) 964, 1037
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 4 cut(s) 314, 315, 396, 517
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 3 cut(s) 170, 386, 528
BseNI ACTGG 2 cut(s) 309, 790
BseX3I CGGCCG 1 cut(s) 1014
BseXI GCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 804
Bsh1236I CGCG 2 cut(s) 175, 863
Bsh1285I CGRYCG 2 cut(s) 940, 1017
BshFI GGCC 4 cut(s) 291, 400, 550, 1016
BsiEI CGRYCG 2 cut(s) 940, 1017
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 5 cut(s) 33, 261, 482, 1013, 1017
BslFI GGGAC 2 cut(s) 451, 660
BslI CCNNNNNNNGG 4 cut(s) 314, 315, 396, 517
BsmAI GTCTC 1 cut(s) 317
BsmFI GGGAC 2 cut(s) 451, 660
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 4 cut(s) 291, 400, 550, 1016
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 641
Bsp143I GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 4 cut(s) 175, 434, 863, 1035
BspANI GGCC 4 cut(s) 291, 400, 550, 1016
BspCNI CTCAG 3 cut(s) 169, 387, 529
BspFNI CGCG 2 cut(s) 175, 863
BspLI GGNNCC 3 cut(s) 573, 711, 759
BspMAI CTGCAG 1 cut(s) 953
BspPI GGATC 5 cut(s) 64, 180, 295, 704, 717
BsrDI GCAATG 1 cut(s) 844
BsrFI RCCGGY 3 cut(s) 32, 1012, 1016
BsrI ACTGG 2 cut(s) 309, 790
BssAI RCCGGY 3 cut(s) 32, 1012, 1016
BssECI CCNNGG 3 cut(s) 511, 642, 751
BssMI GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
BssNI GRCGYC 1 cut(s) 786
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 2 cut(s) 642, 751
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 4 cut(s) 133, 219, 591, 1049
Bst6I CTCTTC 1 cut(s) 244
BstACI GRCGYC 1 cut(s) 786
BstC8I GCNNGC 5 cut(s) 34, 249, 432, 768, 1014
BstDEI CTNAG 3 cut(s) 156, 395, 537
BstDSI CCRYGG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 964, 1037
BstFNI CGCG 2 cut(s) 175, 863
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 7 cut(s) 72, 188, 303, 450, 712, 927, 940
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
BstMCI CGRYCG 2 cut(s) 940, 1017
BstMWI GCNNNNNNNGC 1 cut(s) 942
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 259, 511
BstSFI CTRYAG 2 cut(s) 215, 949
BstUI CGCG 2 cut(s) 175, 863
BstV1I GCAGC 1 cut(s) 539
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 4 cut(s) 69, 185, 300, 709
BstXI CCANNNNNNTGG 2 cut(s) 196, 975
BstYI RGATCY 4 cut(s) 69, 185, 300, 709
BstZI CGGCCG 1 cut(s) 1014
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 4 cut(s) 291, 400, 550, 1016
BtgI CCRYGG 1 cut(s) 642
BtgZI GCGATG 2 cut(s) 747, 945
BtsCI GGATG 2 cut(s) 964, 1037
BtsIMutI CAGTG 4 cut(s) 129, 164, 530, 551
Cac8I GCNNGC 5 cut(s) 34, 249, 432, 768, 1014
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 3 cut(s) 32, 1012, 1016
Cfr13I GGNCC 2 cut(s) 20, 484
CpoI CGGWCCG 1 cut(s) 484
CseI GACGC 1 cut(s) 981
Csp6I GTAC 1 cut(s) 16
CspI CGGWCCG 1 cut(s) 484
CviAII CATG 4 cut(s) 287, 643, 706, 975
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 156, 395, 537
DpnI GATC 7 cut(s) 71, 187, 302, 449, 711, 926, 939
DpnII GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
EaeI YGGCCR 2 cut(s) 289, 1014
EagI CGGCCG 1 cut(s) 1014
Eam1104I CTCTTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 1014
Eco130I CCWWGG 2 cut(s) 642, 751
Eco147I AGGCCT 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 20, 484
Eco52I CGGCCG 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 314
EcoNI CCTNNNNNAGG 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 511
EcoRV GATATC 1 cut(s) 348
EcoT14I CCWWGG 2 cut(s) 642, 751
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 2 cut(s) 642, 751
FaeI CATG 4 cut(s) 290, 646, 709, 978
FaqI GGGAC 2 cut(s) 451, 660
FatI CATG 4 cut(s) 286, 642, 705, 974
FauI CCCGC 1 cut(s) 427
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 2 cut(s) 951, 1044
FriOI GRGCYC 1 cut(s) 641
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 1 cut(s) 504
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 4 cut(s) 291, 400, 550, 1016
HapII CCGG 5 cut(s) 33, 261, 482, 1013, 1017
HgaI GACGC 1 cut(s) 981
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 1 cut(s) 786
Hin1II CATG 4 cut(s) 290, 646, 709, 978
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 3 cut(s) 439, 880, 886
HpaII CCGG 5 cut(s) 33, 261, 482, 1013, 1017
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 5 cut(s) 396, 488, 885, 891, 924
Hpy188III TCNNGA 1 cut(s) 713
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 6 cut(s) 247, 912, 915, 918, 997, 1012
HpyAV CCTTC 2 cut(s) 83, 622
HpyCH4III ACNGT 4 cut(s) 133, 219, 591, 1049
HpyCH4IV ACGT 2 cut(s) 786, 1007
HpyCH4V TGCA 5 cut(s) 332, 461, 810, 821, 951
HpyF10VI GCNNNNNNNGC 1 cut(s) 942
HpyF3I CTNAG 3 cut(s) 156, 395, 537
HpySE526I ACGT 2 cut(s) 786, 1007
Hsp92I GRCGYC 1 cut(s) 786
Hsp92II CATG 4 cut(s) 290, 646, 709, 978
HspAI GCGC 1 cut(s) 173
KroI GCCGGC 2 cut(s) 32, 1012
KroNI GCCGGC 2 cut(s) 34, 1014
Kzo9I GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
LmnI GCTCC 2 cut(s) 177, 571
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 1 cut(s) 1022
MaeI CTAG 1 cut(s) 504
MaeII ACGT 2 cut(s) 786, 1007
MaeIII GTNAC 3 cut(s) 58, 119, 385
MalI GATC 7 cut(s) 71, 187, 302, 449, 711, 926, 939
MboI GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
MboII GAAGA 6 cut(s) 231, 466, 728, 749, 859, 975
MflI RGATCY 4 cut(s) 69, 185, 300, 709
MhlI GDGCHC 1 cut(s) 641
MluCI AATT 5 cut(s) 43, 52, 252, 669, 692
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 7 cut(s) 390, 540, 577, 717, 834, 891, 966
MroNI GCCGGC 2 cut(s) 32, 1012
MslI CAYNNNNRTG 1 cut(s) 973
MspI CCGG 5 cut(s) 33, 261, 482, 1013, 1017
MspR9I CCNGG 2 cut(s) 261, 513
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 1 cut(s) 513
MvnI CGCG 2 cut(s) 175, 863
MwoI GCNNNNNNNGC 1 cut(s) 942
NaeI GCCGGC 2 cut(s) 34, 1014
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 642
NdeII GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
NgoMIV GCCGGC 2 cut(s) 32, 1012
NlaIII CATG 4 cut(s) 290, 646, 709, 978
NlaIV GGNNCC 3 cut(s) 573, 711, 759
NmeAIII GCCGAG 1 cut(s) 317
NmuCI GTSAC 1 cut(s) 119
PceI AGGCCT 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 933
PctI GAATGC 1 cut(s) 979
PdiI GCCGGC 2 cut(s) 34, 1014
PfeI GAWTC 2 cut(s) 880, 886
PkrI GCNGC 1 cut(s) 529
Ple19I CGATCG 1 cut(s) 940
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 573, 711, 759
PspPI GGNCC 2 cut(s) 20, 484
PstI CTGCAG 1 cut(s) 953
PsuI RGATCY 4 cut(s) 69, 185, 300, 709
PvuI CGATCG 1 cut(s) 940
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 973
Rsr2I CGGWCCG 1 cut(s) 484
RsrII CGGWCCG 1 cut(s) 484
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 7 cut(s) 69, 185, 300, 447, 709, 924, 937
Sau96I GGNCC 2 cut(s) 20, 484
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 2 cut(s) 261, 513
SduI GDGCHC 1 cut(s) 641
SfaNI GCATC 1 cut(s) 1022
SfcI CTRYAG 2 cut(s) 215, 949
SinI GGWCC 2 cut(s) 20, 484
SmiMI CAYNNNNRTG 1 cut(s) 973
Sse9I AATT 5 cut(s) 43, 52, 252, 669, 692
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 4 cut(s) 175, 434, 863, 1035
SspMI CTAG 1 cut(s) 504
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 2 cut(s) 259, 511
StyI CCWWGG 2 cut(s) 642, 751
TaaI ACNGT 4 cut(s) 133, 219, 591, 1049
TaiI ACGT 2 cut(s) 789, 1010
TaqI TCGA 2 cut(s) 450, 940
TasI AATT 5 cut(s) 43, 52, 252, 669, 692
TfiI GAWTC 2 cut(s) 880, 886
TscAI CASTG 4 cut(s) 136, 164, 537, 558
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 275, 405
TspGWI ACGGA 3 cut(s) 33, 46, 354
TspRI CASTG 4 cut(s) 136, 164, 537, 558
VpaK11BI GGWCC 2 cut(s) 20, 484
XagI CCTNNNNNAGG 1 cut(s) 394
XapI RAATTY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XspI CTAG 1 cut(s) 504
ZraI GACGTC 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.