AT4G32680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015876
Physical Location & Seq
Reverse (-)
15763237 .. 15765169
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32680.1

Sequence Viewer

Length: 849 bp
ATGGCGTCGAACAGCAGAGAAGCCAGGAGGCGGAAGATCCTAGATAGAGGATCTGATCGTTTAGCCTTTATCACTGGTCAGATCAACGGCGTTCCTTCTCCACCTCCTTCCGATTCTACTTCTTCCCTTTCCCAGTCTGACCTTCAAACTGACCAATCCTTACCGGATACGATTCCCCCTCGCGATCAGATTCTTAAAGCTCAAGAAATCGCTTTCACAAGTCACCAGGACAACATCTCTGATGCTGCAATGCTTGAGAATGTGGATCATATCATCCATCAAAGCAGGGAAGAACCTCTTCAGCCTCAGAGGCATGCTGAAACATTGGCAGAAGCCTCTGCTTCAGATCCTAGAGACACAACAACAATACAACCGCCTCCTACAACATCAAGTGTTCAAAACCCATCAGTGGTAGATTTAGGTGCTTCTCAAGCATTTATTCCTGTGGTTAGTTTTGTGAACGCCATCACACCAAAGCACATTGGAGCGGCCATTGATGCTTCGGAATACGCACGGATGTTCACAGCTCTTGCGATTGCACTTGTTGTCATACTATCTCATCTCGGTTTCTCTTCCCTAGGCAACATTGTAAGCTTCAGGCCAGTTTTCTTGCTTGTCTTGACTGATGCCACAATTGTGCTTGGACGTGTTCTGCTGAGTCATCGTGGAGACTCTTCCTCGGCCTCAGGGACGGTGATGAGCGGGCAGGGTATTGTGGACCAAGTAGGCAACGCTTTGGAGACGGTGATGATGGTGAAGAAGATAATGGATGCACTTCTTATGGATTTTAGCTTATACGCTGTGATTCTCATATGTGGCCTCTTGGTCACCCAAAGCATCTTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

30.2

Weight (kDa)

5.15

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32680
fragaria_vesca FvH4_2g36330
malus_domestica MD15G1099900.v1.1
prunus_persica Prupe.1G454700_v2.0.a1
pyrus_communis pycom08g10100 pycom15g09190
rosa_chinensis RchiOBHm_Chr6g0305551
rosa_multiflora Rmu_sc0000841.1_g000003 Rmu_sc0001153.1_g000006
rosa_roxburghii Rroxscaffold_7G00162580
rosa_rugosa Rorug06G0341600
rosa_samantha Rh6AG453600 Rh6BG448000 Rh6CG467400
rosa_wichuraiana Rw6G039530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 488, 702
AccII CGCG 1 cut(s) 183
AciI CCGC 4 cut(s) 31, 374, 488, 702
AclWI GGATC 4 cut(s) 31, 58, 273, 341
AcoI YGGCCR 1 cut(s) 489
AcuI CTGAAG 3 cut(s) 284, 327, 580
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 30, 409
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 2 cut(s) 146, 398
AjiI CACGTC 1 cut(s) 647
AjnI CCWGG 2 cut(s) 23, 225
AjuI GAANNNNNNNTTGG 1 cut(s) 825
AleI CACNNNNGTG 1 cut(s) 635
AluBI AGCT 4 cut(s) 200, 527, 594, 792
AluI AGCT 4 cut(s) 200, 527, 594, 792
Alw26I GTCTC 3 cut(s) 348, 663, 734
AlwI GGATC 4 cut(s) 31, 58, 273, 341
AoxI GGCC 4 cut(s) 489, 599, 681, 817
ApeKI GCWGC 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 718
AsuHPI GGTGA 5 cut(s) 215, 706, 757, 766, 820
AvaII GGWCC 1 cut(s) 718
AvrII CCTAGG 1 cut(s) 577
AxyI CCTNAGG 1 cut(s) 685
BbvI GCAGC 1 cut(s) 232
BccI CCATC 4 cut(s) 285, 412, 473, 745
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 2 cut(s) 25, 227
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 3 cut(s) 348, 663, 734
BfaI CTAG 3 cut(s) 41, 351, 578
BfuI GTATCC 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 310
BisI GCNGC 2 cut(s) 246, 489
BlnI CCTAGG 1 cut(s) 577
BlsI GCNGC 2 cut(s) 247, 490
Bme1390I CCNGG 2 cut(s) 25, 227
Bme18I GGWCC 1 cut(s) 718
BmgBI CACGTC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 718
BmrFI CCNGG 2 cut(s) 25, 227
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 4 cut(s) 232, 487, 616, 760
BmuI ACTGGG 1 cut(s) 127
BpuEI CTTGAG 3 cut(s) 186, 275, 414
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 577, 678
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 409
Bse1I ACTGG 3 cut(s) 79, 133, 602
Bse21I CCTNAGG 1 cut(s) 685
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 2 cut(s) 25, 227
BseDI CCNNGG 2 cut(s) 577, 678
BseGI GGATG 3 cut(s) 273, 522, 775
BseLI CCNNNNNNNGG 2 cut(s) 30, 409
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 3 cut(s) 320, 647, 699
BseNI ACTGG 3 cut(s) 79, 133, 602
BseXI GCAGC 1 cut(s) 232
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 4 cut(s) 491, 601, 683, 819
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 2 cut(s) 30, 409
BsmAI GTCTC 3 cut(s) 348, 663, 734
BsmBI CGTCTC 1 cut(s) 734
BsmFI GGGAC 1 cut(s) 703
BsnI GGCC 4 cut(s) 491, 601, 683, 819
Bsp143I GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
Bsp68I TCGCGA 1 cut(s) 183
BspACI CCGC 4 cut(s) 31, 374, 488, 702
BspANI GGCC 4 cut(s) 491, 601, 683, 819
BspCNI CTCAG 3 cut(s) 319, 648, 698
BspFNI CGCG 1 cut(s) 183
BspPI GGATC 4 cut(s) 31, 58, 273, 341
BsrBI CCGCTC 2 cut(s) 488, 702
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 79, 133, 602
BssECI CCNNGG 2 cut(s) 577, 678
BssMI GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 577
Bst2UI CCWGG 2 cut(s) 25, 227
Bst4CI ACNGT 2 cut(s) 694, 745
Bst6I CTCTTC 3 cut(s) 303, 577, 679
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 315, 704
BstDEI CTNAG 4 cut(s) 306, 656, 685, 846
BstEII GGTNACC 1 cut(s) 826
BstF5I GGATG 3 cut(s) 273, 522, 775
BstFNI CGCG 1 cut(s) 183
BstKTI GATC 7 cut(s) 39, 53, 58, 84, 187, 268, 349
BstMAI GTCTC 3 cut(s) 348, 663, 734
BstMBI GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
BstMWI GCNNNNNNNGC 3 cut(s) 310, 431, 497
BstNI CCWGG 2 cut(s) 25, 227
BstNSI RCATGY 1 cut(s) 317
BstPI GGTNACC 1 cut(s) 826
BstSCI CCNGG 2 cut(s) 23, 225
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 3 cut(s) 36, 50, 346
BstYI RGATCY 3 cut(s) 36, 50, 346
Bsu36I CCTNAGG 1 cut(s) 685
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 4 cut(s) 491, 601, 683, 819
BtrI CACGTC 1 cut(s) 647
BtsCI GGATG 3 cut(s) 273, 522, 775
BtsIMutI CAGTG 2 cut(s) 72, 414
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 315, 704
Cfr13I GGNCC 1 cut(s) 718
CviAII CATG 1 cut(s) 314
DdeI CTNAG 4 cut(s) 306, 656, 685, 846
DpnI GATC 7 cut(s) 38, 52, 57, 83, 186, 267, 348
DpnII GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 303, 577, 679
EarI CTCTTC 3 cut(s) 303, 577, 679
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 718
Eco57I CTGAAG 3 cut(s) 284, 327, 580
Eco81I CCTNAGG 1 cut(s) 685
Eco91I GGTNACC 1 cut(s) 826
EcoO65I GGTNACC 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 23, 225
EcoT14I CCWWGG 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 577
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 1 cut(s) 317
FaiI YATR 7 cut(s) 270, 315, 551, 782, 796, 812, 814
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 1 cut(s) 703
FatI CATG 1 cut(s) 313
FauI CCCGC 1 cut(s) 695
FauNDI CATATG 1 cut(s) 812
Fnu4HI GCNGC 2 cut(s) 246, 489
FokI GGATG 3 cut(s) 260, 529, 782
Fsp4HI GCNGC 2 cut(s) 246, 489
FspBI CTAG 3 cut(s) 41, 351, 578
GluI GCNGC 2 cut(s) 246, 489
HaeIII GGCC 4 cut(s) 491, 601, 683, 819
HapII CCGG 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 317
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 6 cut(s) 113, 172, 190, 658, 671, 805
HpaII CCGG 1 cut(s) 164
HphI GGTGA 5 cut(s) 215, 706, 757, 766, 820
Hpy166II GTNNAC 3 cut(s) 460, 522, 718
Hpy188I TCNGA 9 cut(s) 55, 81, 112, 139, 189, 241, 309, 346, 505
Hpy188III TCNNGA 3 cut(s) 182, 203, 619
Hpy8I GTNNAC 3 cut(s) 460, 522, 718
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 105, 117, 152
HpyCH4III ACNGT 2 cut(s) 694, 745
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 3 cut(s) 248, 539, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 310, 431, 497
HpyF3I CTNAG 4 cut(s) 306, 656, 685, 846
HpySE526I ACGT 1 cut(s) 646
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 317
Kzo9I GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 4 cut(s) 232, 487, 616, 760
MaeI CTAG 3 cut(s) 41, 351, 578
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 2 cut(s) 221, 826
MalI GATC 7 cut(s) 38, 52, 57, 83, 186, 267, 348
MbiI CCGCTC 2 cut(s) 488, 702
MboI GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
MboII GAAGA 8 cut(s) 46, 114, 290, 302, 564, 666, 769, 772
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 3 cut(s) 36, 50, 346
MluCI AATT 1 cut(s) 633
MlyI GAGTC 2 cut(s) 665, 667
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 1 cut(s) 635
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 25, 227
MunI CAATTG 1 cut(s) 633
MvaI CCWGG 2 cut(s) 25, 227
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 310, 431, 497
NdeI CATATG 1 cut(s) 812
NdeII GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
NlaIII CATG 1 cut(s) 317
NmeAIII GCCGAG 1 cut(s) 659
NmuCI GTSAC 2 cut(s) 221, 826
NruI TCGCGA 1 cut(s) 183
NspI RCATGY 1 cut(s) 317
OliI CACNNNNGTG 1 cut(s) 635
PaeI GCATGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 113, 172, 190, 805
PkrI GCNGC 2 cut(s) 247, 490
PleI GAGTC 2 cut(s) 665, 666
PpsI GAGTC 2 cut(s) 665, 666
Psp6I CCWGG 2 cut(s) 23, 225
PspEI GGTNACC 1 cut(s) 826
PspGI CCWGG 2 cut(s) 23, 225
PspPI GGNCC 1 cut(s) 718
PsuI RGATCY 3 cut(s) 36, 50, 346
RruI TCGCGA 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 635
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 246, 489
Sau3AI GATC 7 cut(s) 36, 50, 55, 81, 184, 265, 346
Sau96I GGNCC 1 cut(s) 718
SchI GAGTC 2 cut(s) 665, 667
ScrFI CCNGG 2 cut(s) 25, 227
SetI ASST 9 cut(s) 106, 144, 202, 298, 424, 529, 596, 649, 794
SfaNI GCATC 4 cut(s) 232, 487, 616, 760
SinI GGWCC 1 cut(s) 718
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 3 cut(s) 201, 254, 429
SmoI CTYRAG 3 cut(s) 201, 254, 429
SphI GCATGC 1 cut(s) 317
Sse9I AATT 1 cut(s) 633
SsiI CCGC 4 cut(s) 31, 374, 488, 702
SspMI CTAG 3 cut(s) 41, 351, 578
StyD4I CCNGG 2 cut(s) 23, 225
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 2 cut(s) 694, 745
TaiI ACGT 1 cut(s) 649
TaqI TCGA 1 cut(s) 8
TasI AATT 1 cut(s) 633
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 4 cut(s) 113, 172, 190, 805
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 2 cut(s) 79, 414
TseFI GTSAC 2 cut(s) 221, 826
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 2 cut(s) 221, 826
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 2 cut(s) 79, 414
VpaK11BI GGWCC 1 cut(s) 718
XceI RCATGY 1 cut(s) 317
XmaJI CCTAGG 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 3 cut(s) 41, 351, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.