MD15G1099900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015876
Physical Location & Seq
Reverse (-)
6943100 .. 6944403
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1099900.v1.1.491

Sequence Viewer

Length: 885 bp
ATGACCACAAACAGCCGAGAAGCCCGACGGAGGAAGATCATGGAGCGAGGGTCCGACCGGCTTGCCCTAATCACGGGTCGGATCCAAACCCTATCCCCCTCCTCCTCTTTATCTTCTTCACCACCGCCAGACCCAGCAACTCACAGTGAAGATACAGACCTGCCATCCCGGCCGTCGATTTCCCCGGATCAGATTCCCAGAACCCACGTCCCAGATCAAATCCCAGTTGCTAATGGCGGTTGTGGGTCTGCAGTTTTCACTCCTGAGATTGAGGAAATGTGGTCAGCACCATCAACGGTTCAGAATGAATCACCGACGTCCCAGACCGAAACCCACGTCCCTGGTCAAATCCCAGTTTTCACTCCTGAGATTGAGGAAAGACGGTCATCATCGTCAACGGTTCAGGATGAATCACCACCATCAACTGTTCAGAATGAGTCACGACCATCATCATTGTCATTTGAGAAGCCTAGACTTTTCACTGCGAGTAGAATAAGTGGCGCCATTGAAGCATCAGAAAGGATCCGAATTTACATTTCCATGGCGATTGCGGTACTGGTGGTTTTTTCCCATCTGGGTTTTCCTTTACTGGGTAGTAAATTGGTGAAAACCCTTATGGGTTTTAGGCCTCTGTACCTGGTTTTGGTGACCAATGTGACACTTGTGCTTGCTCGAATTTTCGACAGCGGTCAACAAAGACACCACAGAGTGCCTGCCGGAATTGGTGAGGGTCAAACTATGAGTGGTAGCGAGTATGAGTGGGCTGAGCGGTTAGGCAAGGCTTTAGGGATAGGCTTGTTGGGGAAGAAGGTACTTGATGCTCTGTTTATGGATTGTGCTGTGTATGCAATAATTGTTGTTTGTGGCCTCTCTTTTGCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

295

Amino Acids

31.91

Weight (kDa)

6.33

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32680
fragaria_vesca FvH4_2g36330
malus_domestica MD15G1099900.v1.1
prunus_persica Prupe.1G454700_v2.0.a1
pyrus_communis pycom08g10100 pycom15g09190
rosa_chinensis RchiOBHm_Chr6g0305551
rosa_multiflora Rmu_sc0000841.1_g000003 Rmu_sc0001153.1_g000006
rosa_roxburghii Rroxscaffold_7G00162580
rosa_rugosa Rorug06G0341600
rosa_samantha Rh6AG453600 Rh6BG448000 Rh6CG467400
rosa_wichuraiana Rw6G039530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 769
AciI CCGC 5 cut(s) 125, 237, 551, 687, 769
AclWI GGATC 5 cut(s) 76, 89, 195, 517, 530
AcoI YGGCCR 1 cut(s) 170
AcsI RAATTY 2 cut(s) 528, 675
AcyI GRCGYC 2 cut(s) 317, 501
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 3 cut(s) 555, 635, 813
AfiI CCNNNNNNNGG 4 cut(s) 30, 73, 590, 643
AgsI TTSAA 1 cut(s) 509
AjiI CACGTC 2 cut(s) 208, 337
AjnI CCWGG 2 cut(s) 340, 636
AlwI GGATC 5 cut(s) 76, 89, 195, 517, 530
AoxI GGCC 3 cut(s) 170, 626, 865
ApoI RAATTY 2 cut(s) 528, 675
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 2 cut(s) 169, 185
AsuHPI GGTGA 6 cut(s) 111, 303, 405, 616, 658, 737
AvaII GGWCC 1 cut(s) 51
BamHI GGATCC 2 cut(s) 81, 522
BanI GGYRCC 1 cut(s) 500
BccI CCATC 5 cut(s) 172, 298, 427, 454, 579
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 2 cut(s) 342, 638
BcnI CCSGG 2 cut(s) 169, 185
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 169
BlpI GCTNAGC 1 cut(s) 765
Bme1390I CCNGG 4 cut(s) 169, 185, 342, 638
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 2 cut(s) 208, 337
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 4 cut(s) 52, 83, 502, 524
BmrFI CCNGG 4 cut(s) 169, 185, 342, 638
BmrI ACTGGG 3 cut(s) 218, 347, 599
BmsI GCATC 2 cut(s) 521, 808
BmuI ACTGGG 3 cut(s) 218, 347, 599
BoxI GACNNNNGTC 1 cut(s) 687
Bpu1102I GCTNAGC 1 cut(s) 765
BpuMI CCSGG 2 cut(s) 169, 185
BsaHI GRCGYC 2 cut(s) 317, 501
BsaJI CCNNGG 3 cut(s) 183, 340, 540
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 73, 590, 643
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 4 cut(s) 224, 353, 561, 594
BseBI CCWGG 2 cut(s) 342, 638
BseDI CCNNGG 3 cut(s) 183, 340, 540
BseGI GGATG 2 cut(s) 164, 412
BseLI CCNNNNNNNGG 4 cut(s) 30, 73, 590, 643
BseMII CTCAG 3 cut(s) 255, 357, 756
BseNI ACTGG 4 cut(s) 224, 353, 561, 594
BseRI GAGGAG 2 cut(s) 91, 94
BseX3I CGGCCG 1 cut(s) 170
BseYI CCCAGC 1 cut(s) 133
Bsh1285I CGRYCG 2 cut(s) 58, 173
BshFI GGCC 3 cut(s) 172, 628, 867
BshNI GGYRCC 1 cut(s) 500
BsiEI CGRYCG 2 cut(s) 58, 173
BsiSI CCGG 4 cut(s) 58, 169, 185, 717
BslFI GGGAC 3 cut(s) 194, 304, 323
BslI CCNNNNNNNGG 4 cut(s) 30, 73, 590, 643
BsmFI GGGAC 3 cut(s) 194, 304, 323
BsnI GGCC 3 cut(s) 172, 628, 867
Bsp143I GATC 5 cut(s) 36, 81, 187, 214, 522
Bsp1720I GCTNAGC 1 cut(s) 765
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 5 cut(s) 125, 237, 551, 687, 769
BspANI GGCC 3 cut(s) 172, 628, 867
BspCNI CTCAG 3 cut(s) 256, 358, 757
BspLI GGNNCC 4 cut(s) 52, 83, 502, 524
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 168
BspPI GGATC 5 cut(s) 76, 89, 195, 517, 530
BspT107I GGYRCC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 769
BsrFI RCCGGY 1 cut(s) 57
BsrI ACTGG 4 cut(s) 224, 353, 561, 594
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 3 cut(s) 183, 340, 540
BssMI GATC 5 cut(s) 36, 81, 187, 214, 522
BssNI GRCGYC 2 cut(s) 317, 501
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 342, 638
Bst4CI ACNGT 5 cut(s) 146, 298, 384, 400, 427
BstACI GRCGYC 2 cut(s) 317, 501
BstC8I GCNNGC 3 cut(s) 63, 669, 714
BstDEI CTNAG 4 cut(s) 264, 366, 765, 879
BstDSI CCRYGG 1 cut(s) 540
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 2 cut(s) 164, 412
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 5 cut(s) 39, 84, 190, 217, 525
BstMBI GATC 5 cut(s) 36, 81, 187, 214, 522
BstMCI CGRYCG 2 cut(s) 58, 173
BstMWI GCNNNNNNNGC 3 cut(s) 169, 509, 845
BstNI CCWGG 2 cut(s) 342, 638
BstPAI GACNNNNGTC 1 cut(s) 687
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 4 cut(s) 167, 183, 340, 636
BstSFI CTRYAG 1 cut(s) 249
BstX2I RGATCY 2 cut(s) 81, 522
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 2 cut(s) 81, 522
BstZI CGGCCG 1 cut(s) 170
BsuRI GGCC 3 cut(s) 172, 628, 867
BtgI CCRYGG 1 cut(s) 540
BtrI CACGTC 2 cut(s) 208, 337
BtsCI GGATG 2 cut(s) 164, 412
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 151, 480
BveI ACCTGC 1 cut(s) 168
Cac8I GCNNGC 3 cut(s) 63, 669, 714
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 51
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 3 cut(s) 554, 634, 812
CviAII CATG 2 cut(s) 40, 541
CviQI GTAC 3 cut(s) 554, 634, 812
DdeI CTNAG 4 cut(s) 264, 366, 765, 879
DinI GGCGCC 1 cut(s) 502
DpnI GATC 5 cut(s) 38, 83, 189, 216, 524
DpnII GATC 5 cut(s) 36, 81, 187, 214, 522
DraIII CACNNNGTG 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 170
EagI CGGCCG 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 540
Eco147I AGGCCT 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 51
Eco52I CGGCCG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 646
EcoRII CCWGG 2 cut(s) 340, 636
EcoT14I CCWWGG 1 cut(s) 540
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 2 cut(s) 43, 544
FaiI YATR 7 cut(s) 41, 542, 617, 740, 756, 830, 846
FaqI GGGAC 3 cut(s) 194, 304, 323
FatI CATG 2 cut(s) 39, 540
FokI GGATG 2 cut(s) 151, 419
FspBI CTAG 1 cut(s) 471
GlaI GCGC 1 cut(s) 502
GsaI CCCAGC 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 3 cut(s) 172, 628, 867
HapII CCGG 4 cut(s) 58, 169, 185, 717
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 2 cut(s) 317, 501
Hin1II CATG 2 cut(s) 43, 544
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HincII GTYRAC 2 cut(s) 396, 692
HindII GTYRAC 2 cut(s) 396, 692
HinfI GANTC 4 cut(s) 193, 308, 410, 437
HpaII CCGG 4 cut(s) 58, 169, 185, 717
HphI GGTGA 6 cut(s) 111, 303, 405, 616, 658, 737
Hpy166II GTNNAC 2 cut(s) 396, 692
Hpy188I TCNGA 7 cut(s) 55, 81, 192, 303, 432, 517, 527
Hpy188III TCNNGA 4 cut(s) 263, 365, 404, 441
Hpy8I GTNNAC 2 cut(s) 396, 692
Hpy99I CGWCG 3 cut(s) 30, 178, 319
HpyAV CCTTC 1 cut(s) 802
HpyCH4III ACNGT 5 cut(s) 146, 298, 384, 400, 427
HpyCH4IV ACGT 3 cut(s) 207, 317, 336
HpyCH4V TGCA 2 cut(s) 251, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 509, 845
HpyF3I CTNAG 4 cut(s) 264, 366, 765, 879
HpySE526I ACGT 3 cut(s) 207, 317, 336
Hsp92I GRCGYC 2 cut(s) 317, 501
Hsp92II CATG 2 cut(s) 43, 544
HspAI GCGC 1 cut(s) 501
KasI GGCGCC 1 cut(s) 500
Kzo9I GATC 5 cut(s) 36, 81, 187, 214, 522
LmnI GCTCC 1 cut(s) 43
LweI GCATC 2 cut(s) 521, 808
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 1 cut(s) 471
MaeII ACGT 3 cut(s) 207, 317, 336
MaeIII GTNAC 3 cut(s) 438, 646, 655
MalI GATC 5 cut(s) 38, 83, 189, 216, 524
MbiI CCGCTC 1 cut(s) 769
MboI GATC 5 cut(s) 36, 81, 187, 214, 522
MboII GAAGA 5 cut(s) 46, 105, 108, 161, 817
MflI RGATCY 2 cut(s) 81, 522
MluCI AATT 5 cut(s) 528, 599, 675, 720, 852
Mly113I GGCGCC 1 cut(s) 501
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 2 cut(s) 59, 78
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 687
MspI CCGG 4 cut(s) 58, 169, 185, 717
MspR9I CCNGG 4 cut(s) 169, 185, 342, 638
MvaI CCWGG 2 cut(s) 342, 638
MwoI GCNNNNNNNGC 3 cut(s) 169, 509, 845
NarI GGCGCC 1 cut(s) 501
NciI CCSGG 2 cut(s) 169, 185
NcoI CCATGG 1 cut(s) 540
NdeII GATC 5 cut(s) 36, 81, 187, 214, 522
NlaIII CATG 2 cut(s) 43, 544
NlaIV GGNNCC 4 cut(s) 52, 83, 502, 524
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 3 cut(s) 438, 646, 655
PceI AGGCCT 1 cut(s) 628
PfeI GAWTC 3 cut(s) 193, 308, 410
PleI GAGTC 1 cut(s) 445
PluTI GGCGCC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 445
PshAI GACNNNNGTC 1 cut(s) 687
Psp6I CCWGG 2 cut(s) 340, 636
PspEI GGTNACC 1 cut(s) 646
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 2 cut(s) 340, 636
PspN4I GGNNCC 4 cut(s) 52, 83, 502, 524
PspPI GGNCC 1 cut(s) 51
PstI CTGCAG 1 cut(s) 253
PsuI RGATCY 2 cut(s) 81, 522
RsaI GTAC 3 cut(s) 555, 635, 813
RsaNI GTAC 3 cut(s) 554, 634, 812
RseI CAYNNNNRTG 1 cut(s) 539
Sau3AI GATC 5 cut(s) 36, 81, 187, 214, 522
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 4 cut(s) 169, 185, 342, 638
SetI ASST 6 cut(s) 162, 210, 320, 339, 639, 813
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 2 cut(s) 521, 808
SfcI CTRYAG 1 cut(s) 249
SfoI GGCGCC 1 cut(s) 502
SinI GGWCC 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 539
Sse9I AATT 5 cut(s) 528, 599, 675, 720, 852
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 5 cut(s) 125, 237, 551, 687, 769
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 1 cut(s) 471
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 4 cut(s) 167, 183, 340, 636
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 5 cut(s) 146, 298, 384, 400, 427
TaiI ACGT 3 cut(s) 210, 320, 339
TaqI TCGA 3 cut(s) 176, 673, 681
TaqII GACCGA 1 cut(s) 341
TasI AATT 5 cut(s) 528, 599, 675, 720, 852
TfiI GAWTC 3 cut(s) 193, 308, 410
TscAI CASTG 2 cut(s) 151, 487
TseFI GTSAC 3 cut(s) 438, 646, 655
Tsp45I GTSAC 3 cut(s) 438, 646, 655
TspDTI ATGAA 2 cut(s) 321, 423
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 2 cut(s) 151, 487
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 2 cut(s) 528, 675
XspI CTAG 1 cut(s) 471
ZraI GACGTC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.