AT4G33110

rRNA small subunit methyltransferase G

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010879
Physical Location & Seq
Reverse (-)
15972323 .. 15974797
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33110.1

Sequence Viewer

Length: 1068 bp
ATGGAGAAGATTATTGACGTAGCTTATGGCGCATCAGTGAAGGTGGGTTTGACGTTGTTGGAGATGAATCTGTTGCCGGATATTGTCATTAGGCGGCTCACACGTCTGCTTCTGGCCGGTCGTCTCCGTTCCGGTTACAAACCCACGGCGGAGATGCAACTCTCCGATCTCCTCCGTTTTGTTGACTCTATAAAGAAGATGCCTATAGCTATCCATACCGAGAAGCCAAAGACTCAACACTATGAATTACCGACAGCTTTCTTCGAACTTGTCCTTGGAAGAAACATGAAGTACAGCTCTTGTTACTTCTCAAACGATTCAAGTAGCTTAGAAGATGCAGAGGAAGCAATTTTGGCTCTTTATTGTGAAAGAGCTAAAGTGGAAGATGGTCAAAGTGTTCTTGATATCGGATGTGGCTGGGGATCCTTGTCTTTGTACATTGCACGCAAGTACAGCAAATGCAAGTTAACCGGTATCTGCAACTCCAAAACGCAGAAAGCATTTATCGATGAGAAATGCCGGAAACTCGGGCTTCAGAATATCGAAATCATTGTCGCGGATATTAGCACTTTTGAGCATGAAGGGACATACGATCGGATATTCTCCATCGAGATGTTTGAGCATATGAAAAACTATGGAGAGCTACTAAAGAAGATAGGAAAGTGGATGAAGGAAGATAGTCTTCTGTTTGTTCACTATTTCTGCCATAAGACATTTGCTTACCACTTTGAGGATGTGAATGACGATGACTGGATCACAAGACACTTCTTCAGCGGAGGAACAATGCCATCAGCAGATCTTCTGCTATATTTTCAAGAAAATGTTTCGATTGTGGATCATTGGCTCGTAAGCGGGACGCATTATGCAAAAACAAGTGAAGAGTGGCTAAAAAGAATGGATAAGGAGATAGTTGCAGTAAAAAAAATCATGGAAGTGACTTATGGGAAAGAAGAAGCAGTGAAGTGGATGGTTTACTGGAGAACCTTCTTCATCGCGGTTGCTGAGCTTTTCGGATACAACAATGGAGAAGAGTGGATGGTTGCACACTTCCTCTTCAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

355

Amino Acids

41.23

Weight (kDa)

6.43

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 77 - 327 4e-42 Mycolic acid cyclopropane synthetase
MTS PF05175 126 - 201 4e-06 Methyltransferase small domain
Methyltransf_23 PF13489 127 - 237 3.1e-13 Methyltransferase domain
GidB PF02527 128 - 223 7.4e-07 rRNA small subunit methyltransferase G
Methyltransf_31 PF13847 129 - 247 1.6e-13 Methyltransferase domain
Methyltransf_25 PF13649 133 - 225 5.9e-14 Methyltransferase domain
Methyltransf_11 PF08241 134 - 230 1e-11 Methyltransferase domain
Methyltransf_12 PF08242 134 - 227 1.6e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 557, 995
AciI CCGC 6 cut(s) 94, 149, 557, 774, 852, 995
AclWI GGATC 4 cut(s) 417, 430, 761, 843
AcoI YGGCCR 1 cut(s) 114
AcuI CTGAAG 2 cut(s) 518, 754
AfaI GTAC 3 cut(s) 293, 437, 452
AfiI CCNNNNNNNGG 1 cut(s) 730
AflIII ACRYGT 1 cut(s) 101
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 3 cut(s) 321, 815, 1057
AjiI CACGTC 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 8 cut(s) 23, 209, 257, 297, 327, 374, 643, 1006
AluI AGCT 8 cut(s) 23, 209, 257, 297, 327, 374, 643, 1006
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 4 cut(s) 417, 430, 761, 843
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AsiGI ACCGGT 1 cut(s) 470
AspLEI GCGC 1 cut(s) 32
AsuII TTCGAA 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 527
BamHI GGATCC 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 674
BccI CCATC 5 cut(s) 380, 614, 796, 961, 1030
BceAI ACGGC 1 cut(s) 162
BciVI GTATCC 1 cut(s) 1007
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 204
BfuI GTATCC 1 cut(s) 1007
BglII AGATCT 1 cut(s) 796
BisI GCNGC 1 cut(s) 95
BlpI GCTNAGC 1 cut(s) 1002
BlsI GCNGC 1 cut(s) 96
BmeT110I CYCGRG 1 cut(s) 527
BmgBI CACGTC 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 424
BmsI GCATC 4 cut(s) 41, 144, 189, 325
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 997
Bpu1102I GCTNAGC 1 cut(s) 1002
Bpu14I TTCGAA 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 144, 274
BsaWI WCCGGW 2 cut(s) 131, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 730
Bse118I RCCGGY 2 cut(s) 116, 470
Bse1I ACTGG 2 cut(s) 755, 980
Bse3DI GCAATG 1 cut(s) 438
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 144, 274
BseGI GGATG 5 cut(s) 416, 672, 739, 972, 1041
BseLI CCNNNNNNNGG 1 cut(s) 730
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 993
BseNI ACTGG 2 cut(s) 755, 980
BseRI GAGGAG 1 cut(s) 161
BseYI CCCAGC 1 cut(s) 417
Bsh1236I CGCG 2 cut(s) 557, 995
Bsh1285I CGRYCG 2 cut(s) 121, 595
BshFI GGCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 2 cut(s) 121, 595
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 5 cut(s) 77, 117, 132, 471, 520
BslFI GGGAC 2 cut(s) 598, 868
BslI CCNNNNNNNGG 1 cut(s) 730
BsmAI GTCTC 1 cut(s) 128
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 2 cut(s) 598, 868
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 527
Bsp119I TTCGAA 1 cut(s) 264
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 6 cut(s) 166, 422, 592, 753, 796, 835
Bsp1720I GCTNAGC 1 cut(s) 1002
BspACI CCGC 6 cut(s) 94, 149, 557, 774, 852, 995
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 994
BspDI ATCGAT 1 cut(s) 507
BspFNI CGCG 2 cut(s) 557, 995
BspLI GGNNCC 1 cut(s) 424
BspPI GGATC 4 cut(s) 417, 430, 761, 843
BspT104I TTCGAA 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 2 cut(s) 116, 470
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 2 cut(s) 755, 980
BssAI RCCGGY 2 cut(s) 116, 470
BssECI CCNNGG 2 cut(s) 144, 274
BssMI GATC 6 cut(s) 166, 422, 592, 753, 796, 835
BssT1I CCWWGG 1 cut(s) 274
Bst6I CTCTTC 3 cut(s) 873, 1023, 1058
BstAUI TGTACA 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 328, 1002
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 5 cut(s) 416, 672, 739, 972, 1041
BstFNI CGCG 2 cut(s) 557, 995
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 6 cut(s) 169, 425, 595, 756, 799, 838
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 6 cut(s) 166, 422, 592, 753, 796, 835
BstMCI CGRYCG 2 cut(s) 121, 595
BstMWI GCNNNNNNNGC 4 cut(s) 29, 344, 353, 453
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 2 cut(s) 557, 995
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 2 cut(s) 422, 796
BstYI RGATCY 2 cut(s) 422, 796
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 976
BtrI CACGTC 1 cut(s) 104
BtsCI GGATG 5 cut(s) 416, 672, 739, 972, 1041
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 2 cut(s) 42, 963
Cac8I GCNNGC 1 cut(s) 445
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 2 cut(s) 116, 470
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 865
Csp6I GTAC 3 cut(s) 292, 436, 451
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 3 cut(s) 286, 578, 928
CviQI GTAC 3 cut(s) 292, 436, 451
DdeI CTNAG 2 cut(s) 328, 1002
DpnI GATC 6 cut(s) 168, 424, 594, 755, 798, 837
DpnII GATC 6 cut(s) 166, 422, 592, 753, 796, 835
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 3 cut(s) 873, 1023, 1058
EarI CTCTTC 3 cut(s) 873, 1023, 1058
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 274
Eco32I GATATC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 518, 754
Eco88I CYCGRG 1 cut(s) 527
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 3 cut(s) 289, 581, 931
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 2 cut(s) 598, 868
FatI CATG 3 cut(s) 285, 577, 927
FauI CCCGC 1 cut(s) 845
FauNDI CATATG 1 cut(s) 624
Fnu4HI GCNGC 1 cut(s) 95
FokI GGATG 5 cut(s) 423, 679, 746, 979, 1048
Fsp4HI GCNGC 1 cut(s) 95
GlaI GCGC 1 cut(s) 31
GluI GCNGC 1 cut(s) 95
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 997
HaeIII GGCC 1 cut(s) 116
HapII CCGG 5 cut(s) 77, 117, 132, 471, 520
HgaI GACGC 1 cut(s) 865
HhaI GCGC 1 cut(s) 32
Hin1II CATG 3 cut(s) 289, 581, 931
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 2 cut(s) 184, 468
HindII GTYRAC 2 cut(s) 184, 468
HinfI GANTC 4 cut(s) 67, 185, 232, 317
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 5 cut(s) 77, 117, 132, 471, 520
Hpy166II GTNNAC 4 cut(s) 184, 468, 694, 973
Hpy188I TCNGA 5 cut(s) 166, 410, 537, 597, 1013
Hpy188III TCNNGA 4 cut(s) 401, 610, 815, 1057
Hpy8I GTNNAC 4 cut(s) 184, 468, 694, 973
HpyAV CCTTC 4 cut(s) 34, 575, 664, 994
HpyCH4IV ACGT 3 cut(s) 18, 53, 103
HpyCH4V TGCA 8 cut(s) 157, 338, 443, 462, 480, 866, 914, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 344, 353, 453
HpyF3I CTNAG 2 cut(s) 328, 1002
HpySE526I ACGT 3 cut(s) 18, 53, 103
Hsp92II CATG 3 cut(s) 289, 581, 931
HspAI GCGC 1 cut(s) 30
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 6 cut(s) 166, 422, 592, 753, 796, 835
LpnPI CCDG 9 cut(s) 90, 98, 130, 145, 403, 484, 533, 736, 961
LweI GCATC 4 cut(s) 41, 144, 189, 325
MaeII ACGT 3 cut(s) 18, 53, 103
MaeIII GTNAC 3 cut(s) 134, 302, 934
MalI GATC 6 cut(s) 168, 424, 594, 755, 798, 837
MboI GATC 6 cut(s) 166, 422, 592, 753, 796, 835
MflI RGATCY 2 cut(s) 422, 796
MluCI AATT 2 cut(s) 245, 348
MlyI GAGTC 2 cut(s) 179, 226
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 5 cut(s) 182, 334, 724, 770, 1061
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 2 cut(s) 611, 932
MspA1I CMGCKG 1 cut(s) 774
MspI CCGG 5 cut(s) 77, 117, 132, 471, 520
MvnI CGCG 2 cut(s) 557, 995
MwoI GCNNNNNNNGC 4 cut(s) 29, 344, 353, 453
NdeI CATATG 1 cut(s) 624
NdeII GATC 6 cut(s) 166, 422, 592, 753, 796, 835
NlaIII CATG 3 cut(s) 289, 581, 931
NlaIV GGNNCC 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 934
NspV TTCGAA 1 cut(s) 264
PfeI GAWTC 2 cut(s) 67, 317
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 1 cut(s) 96
Ple19I CGATCG 1 cut(s) 595
PleI GAGTC 2 cut(s) 179, 226
PpsI GAGTC 2 cut(s) 179, 226
PspFI CCCAGC 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 424
PsuI RGATCY 2 cut(s) 422, 796
PvuI CGATCG 1 cut(s) 595
RsaI GTAC 3 cut(s) 293, 437, 452
RsaNI GTAC 3 cut(s) 292, 436, 451
RseI CAYNNNNRTG 2 cut(s) 611, 932
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 1 cut(s) 95
Sau3AI GATC 6 cut(s) 166, 422, 592, 753, 796, 835
SchI GAGTC 2 cut(s) 179, 226
SfaNI GCATC 4 cut(s) 41, 144, 189, 325
SfcI CTRYAG 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 611, 932
Sse9I AATT 2 cut(s) 245, 348
SsiI CCGC 6 cut(s) 94, 149, 557, 774, 852, 995
StyI CCWWGG 1 cut(s) 274
TaiI ACGT 3 cut(s) 21, 56, 106
TaqI TCGA 5 cut(s) 264, 507, 543, 609, 827
TasI AATT 2 cut(s) 245, 348
TatI WGTACW 3 cut(s) 291, 435, 450
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 2 cut(s) 67, 317
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 2 cut(s) 42, 963
TseFI GTSAC 1 cut(s) 934
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 7 cut(s) 80, 258, 302, 594, 641, 683, 979
TspGWI ACGGA 2 cut(s) 116, 164
TspRI CASTG 2 cut(s) 42, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.