FvH4_2g31420

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010879
Physical Location & Seq
Forward (+)
24077400 .. 24079860
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31420.t1

Sequence Viewer

Length: 1068 bp
ATGGATCGAGTAATGCAGGCACCCTACGATGCTACCGTCCGATTCGCTCTGGCGTCACTGGAAAGGAATCTGTTGCCGGACGCCGTTATACGACGGTTCACGCGCTTGCTCTTAGCTAGCCGGCTACGCACCGGTTACCAGCCTTCCTCTGAGCTCCAGCTCTCCCAACTCCTCCAGTTCGTTAAGTCTCTGAAGGAGATGCCAATAGCTATCAAGACTGATGATGCTAAAGCTCAACACTATGAAGTACCGACTTCTTTCTTCAAGATGGTGCTTGGAAACAATCTCAAATATAGTTGTTGTTACTTCACTGATAAGTCAAGCAGCTTGGAGGATGCTGAGAAGGCCATGTTGGAGATGTACTGTGAGAGGTCGCAGATTAAAGATGGCGATAGTGTTCTTGATGTGGGGTGCGGATGGGGATCACTGTCTCTGTACATTGCTGAGAAGTATAAGAATTGCAAGGTTACTGGGATATGCAATTCAGCAACACAGAAGGCTTTTATAGAAGAGCAGTGTAGGAATCGTCAGCTGGAGAATGTGGAGATCATTGTTGCAGATATCAGCACATTTGAGATGGAGGCATCATATGACAGAATATTTTCCATTGAAATGTTTGAGCACATGAAGAACTACAAGGATCTTCTGAAGAAGATATCCGGGTGGATGAAACAGGACGGCCTTCTGTTTGTCCATCATTTCTGTCACAAAGCATTTGCTTACCACTTTGAGGATAAAAGTGATGATGACTGGATTACTAGATACTTCTTCAGTGGAGGTACAATGCCTTCGGCAAATCTACTACTGTATTTCCAGGATGATGTTTCTGTTGTCGATCATTGGCTTGTCAATGGGAAGCATTATGCACAAACAAGTGAAGAATGGCTGAAGAGAATGGACCATAACTCAGCTGCCATAAAACCAATTATGGAGTCAACTTATGGCAAGGATTCAGCTGTGAAGTGGACTGTGTATTGGAGAACTTTCTTTATTGCGGTTGCAGAACTCTTTGGCTACAACAACGGAGAAGAATGGATGGTTGCTCTCTTCCTATTCAAAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

356

Amino Acids

41.22

Weight (kDa)

6.17

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 75 - 311 1.4e-46 Mycolic acid cyclopropane synthetase
PCMT PF01135 116 - 201 4.8e-06 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
TRM5-TYW2_MTfase PF02475 125 - 238 3.1e-06 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 127 - 248 6.5e-16 Methyltransferase domain
Methyltransf_23 PF13489 127 - 235 2.3e-12 Methyltransferase domain
MTS PF05175 129 - 202 1.7e-07 Methyltransferase small domain
TehB PF03848 130 - 213 1.6e-06 Tellurite resistance protein TehB
Methyltransf_25 PF13649 133 - 227 6.1e-16 Methyltransferase domain
Methyltransf_11 PF08241 134 - 231 5.7e-15 Methyltransferase domain
Methyltransf_12 PF08242 134 - 229 2.3e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 1 cut(s) 103
AciI CCGC 2 cut(s) 414, 995
AclWI GGATC 3 cut(s) 12, 430, 648
AcuI CTGAAG 4 cut(s) 212, 668, 754, 908
AcyI GRCGYC 2 cut(s) 53, 81
AfaI GTAC 4 cut(s) 249, 362, 437, 781
AfiI CCNNNNNNNGG 1 cut(s) 730
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 3 cut(s) 265, 611, 1057
AjnI CCWGG 1 cut(s) 813
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 9 cut(s) 116, 154, 160, 209, 233, 327, 532, 911, 956
AluI AGCT 9 cut(s) 116, 154, 160, 209, 233, 327, 532, 911, 956
Alw21I GWGCWC 2 cut(s) 156, 624
Alw26I GTCTC 2 cut(s) 192, 435
AlwI GGATC 3 cut(s) 12, 430, 648
AoxI GGCC 2 cut(s) 345, 679
ApeKI GCWGC 2 cut(s) 324, 911
AsiGI ACCGGT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 601
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 898
AsuC2I CCSGG 1 cut(s) 661
AsuNHI GCTAGC 1 cut(s) 116
AvaII GGWCC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 156
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
Bbv12I GWGCWC 2 cut(s) 156, 624
BbvI GCAGC 2 cut(s) 336, 898
BccI CCATC 6 cut(s) 262, 380, 411, 571, 702, 1030
BceAI ACGGC 2 cut(s) 68, 694
BciT130I CCWGG 1 cut(s) 815
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 192, 435
BfaI CTAG 2 cut(s) 117, 759
BisI GCNGC 2 cut(s) 325, 912
BlsI GCNGC 2 cut(s) 326, 913
Bme1390I CCNGG 2 cut(s) 661, 815
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 2 cut(s) 661, 815
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 5 cut(s) 19, 189, 214, 325, 593
BmtI GCTAGC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 480
BpmI CTGGAG 3 cut(s) 140, 158, 554
BpuMI CCSGG 1 cut(s) 661
BsaBI GATNNNNATC 1 cut(s) 421
BsaHI GRCGYC 2 cut(s) 53, 81
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 1 cut(s) 730
Bse118I RCCGGY 2 cut(s) 120, 131
Bse1I ACTGG 4 cut(s) 63, 175, 475, 755
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 1 cut(s) 815
BseGI GGATG 5 cut(s) 340, 422, 672, 823, 1041
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 730
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 4 cut(s) 141, 330, 435, 921
BseNI ACTGG 4 cut(s) 63, 175, 475, 755
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 2 cut(s) 336, 898
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 347, 681
BshNI GGYRCC 1 cut(s) 19
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 156, 624
BsiSI CCGG 4 cut(s) 77, 121, 132, 660
BslI CCNNNNNNNGG 1 cut(s) 730
BsmAI GTCTC 2 cut(s) 192, 435
BsnI GGCC 2 cut(s) 347, 681
Bsp1286I GDGCHC 2 cut(s) 156, 624
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 5 cut(s) 4, 422, 546, 640, 835
BspACI CCGC 2 cut(s) 414, 995
BspANI GGCC 2 cut(s) 347, 681
BspCNI CTCAG 4 cut(s) 142, 331, 436, 920
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 21
BspOI GCTAGC 1 cut(s) 120
BspPI GGATC 3 cut(s) 12, 430, 648
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 2 cut(s) 120, 131
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 4 cut(s) 63, 175, 475, 755
BssAI RCCGGY 2 cut(s) 120, 131
BssMI GATC 5 cut(s) 4, 422, 546, 640, 835
BssNI GRCGYC 2 cut(s) 53, 81
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 6 cut(s) 37, 96, 365, 429, 807, 970
Bst6I CTCTTC 3 cut(s) 504, 884, 1052
BstACI GRCGYC 2 cut(s) 53, 81
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 4 cut(s) 18, 107, 118, 122
BstDEI CTNAG 5 cut(s) 112, 150, 339, 444, 907
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 5 cut(s) 340, 422, 672, 823, 1041
BstFNI CGCG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 5 cut(s) 7, 425, 549, 643, 838
BstMAI GTCTC 2 cut(s) 192, 435
BstMBI GATC 5 cut(s) 4, 422, 546, 640, 835
BstMWI GCNNNNNNNGC 2 cut(s) 126, 344
BstNI CCWGG 1 cut(s) 815
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 659, 813
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 336, 898
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 2 cut(s) 347, 681
BtsCI GGATG 5 cut(s) 340, 422, 672, 823, 1041
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 5 cut(s) 56, 309, 425, 521, 778
Cac8I GCNNGC 4 cut(s) 18, 107, 118, 122
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 2 cut(s) 120, 131
Cfr13I GGNCC 1 cut(s) 898
CseI GACGC 2 cut(s) 42, 89
Csp6I GTAC 4 cut(s) 248, 361, 436, 780
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 2 cut(s) 349, 625
CviQI GTAC 4 cut(s) 248, 361, 436, 780
DdeI CTNAG 5 cut(s) 112, 150, 339, 444, 907
DpnI GATC 5 cut(s) 6, 424, 548, 642, 837
DpnII GATC 5 cut(s) 4, 422, 546, 640, 835
Eam1104I CTCTTC 3 cut(s) 504, 884, 1052
EarI CTCTTC 3 cut(s) 504, 884, 1052
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco32I GATATC 2 cut(s) 562, 657
Eco47I GGWCC 1 cut(s) 898
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 4 cut(s) 212, 668, 754, 908
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 813
EcoRV GATATC 2 cut(s) 562, 657
EcoT38I GRGCYC 1 cut(s) 156
FaeI CATG 2 cut(s) 352, 628
FatI CATG 2 cut(s) 348, 624
FauNDI CATATG 1 cut(s) 589
Fnu4HI GCNGC 2 cut(s) 325, 912
FokI GGATG 5 cut(s) 347, 429, 679, 830, 1048
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 2 cut(s) 325, 912
FspBI CTAG 2 cut(s) 117, 759
GlaI GCGC 1 cut(s) 104
GluI GCNGC 2 cut(s) 325, 912
GsuI CTGGAG 3 cut(s) 140, 158, 554
HaeIII GGCC 2 cut(s) 347, 681
HapII CCGG 4 cut(s) 77, 121, 132, 660
HgaI GACGC 2 cut(s) 42, 89
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 2 cut(s) 53, 81
Hin1II CATG 2 cut(s) 352, 628
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HinfI GANTC 5 cut(s) 42, 67, 523, 932, 950
HpaII CCGG 4 cut(s) 77, 121, 132, 660
Hpy166II GTNNAC 3 cut(s) 99, 936, 966
Hpy188I TCNGA 4 cut(s) 41, 151, 192, 648
Hpy188III TCNNGA 3 cut(s) 214, 265, 401
Hpy8I GTNNAC 3 cut(s) 99, 936, 966
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 6 cut(s) 153, 187, 337, 490, 692, 798
HpyCH4III ACNGT 6 cut(s) 37, 96, 365, 429, 807, 970
HpyCH4V TGCA 6 cut(s) 16, 462, 480, 557, 866, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 344
HpyF3I CTNAG 5 cut(s) 112, 150, 339, 444, 907
Hsp92I GRCGYC 2 cut(s) 53, 81
Hsp92II CATG 2 cut(s) 352, 628
HspAI GCGC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 120
KroNI GCCGGC 1 cut(s) 122
Kzo9I GATC 5 cut(s) 4, 422, 546, 640, 835
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 2 cut(s) 336, 898
LweI GCATC 5 cut(s) 19, 189, 214, 325, 593
MaeI CTAG 2 cut(s) 117, 759
MaeIII GTNAC 5 cut(s) 54, 134, 302, 466, 704
MalI GATC 5 cut(s) 6, 424, 548, 642, 837
MboI GATC 5 cut(s) 4, 422, 546, 640, 835
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 156, 624
MluCI AATT 3 cut(s) 457, 481, 924
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 7 cut(s) 157, 182, 325, 363, 574, 724, 770
MroNI GCCGGC 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 601
MseI TTAA 2 cut(s) 183, 381
MslI CAYNNNNRTG 1 cut(s) 611
MspA1I CMGCKG 3 cut(s) 532, 911, 956
MspI CCGG 4 cut(s) 77, 121, 132, 660
MspR9I CCNGG 2 cut(s) 661, 815
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 126, 344
NaeI GCCGGC 1 cut(s) 122
NciI CCSGG 1 cut(s) 661
NdeI CATATG 1 cut(s) 589
NdeII GATC 5 cut(s) 4, 422, 546, 640, 835
NgoMIV GCCGGC 1 cut(s) 120
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 2 cut(s) 352, 628
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 54, 704
PciSI GCTCTTC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdiI GCCGGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 42, 67, 523, 950
PfoI TCCNGGA 1 cut(s) 813
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 2 cut(s) 326, 913
PleI GAGTC 1 cut(s) 940
PpsI GAGTC 1 cut(s) 940
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 813
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 898
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 3 cut(s) 532, 911, 956
RsaI GTAC 4 cut(s) 249, 362, 437, 781
RsaNI GTAC 4 cut(s) 248, 361, 436, 780
RseI CAYNNNNRTG 1 cut(s) 611
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 2 cut(s) 183, 381
SatI GCNGC 2 cut(s) 325, 912
Sau3AI GATC 5 cut(s) 4, 422, 546, 640, 835
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 2 cut(s) 661, 815
SduI GDGCHC 2 cut(s) 156, 624
SfaNI GCATC 5 cut(s) 19, 189, 214, 325, 593
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 1 cut(s) 611
Sse9I AATT 3 cut(s) 457, 481, 924
SsiI CCGC 2 cut(s) 414, 995
SspI AATATT 1 cut(s) 600
SspMI CTAG 2 cut(s) 117, 759
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 659, 813
TaaI ACNGT 6 cut(s) 37, 96, 365, 429, 807, 970
TaqI TCGA 2 cut(s) 7, 834
TasI AATT 3 cut(s) 457, 481, 924
TatI WGTACW 2 cut(s) 360, 435
TfiI GAWTC 4 cut(s) 42, 67, 523, 950
Tru1I TTAA 2 cut(s) 183, 381
Tru9I TTAA 2 cut(s) 183, 381
TscAI CASTG 5 cut(s) 63, 316, 432, 521, 778
TseFI GTSAC 2 cut(s) 54, 704
TseI GCWGC 2 cut(s) 324, 911
Tsp45I GTSAC 2 cut(s) 54, 704
TspDTI ATGAA 3 cut(s) 258, 641, 683
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 5 cut(s) 63, 316, 432, 521, 778
VpaK11BI GGWCC 1 cut(s) 898
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 2 cut(s) 117, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.