MD15G1054300.v1.1

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010879
Physical Location & Seq
Forward (+)
3699925 .. 3702433
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1054300.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGAACAGAGTAATGCAAGCACCGTACGATGCTACCGTCCGGTTCGCGCTGGCTTCGCTGGAGCGGAATCTGCTGCCGGACGCCGTCGTGAGGCGGCTGACACGGCTGCTCTTGGCGAGCCGTCTCCGGTCCGGTTACAGGCCTTCCTCCGAGCTCCAGCTTTCCGACCTCCTTCAATTCGTGCAATCTTTGAAGGAGATGCCCATAGCCATAAAGACTGATGATCCAAAAGCTCAACACTATGAAGTTCCCACATCTTTTTTCAAGATGGTGCTTGGGAAAAATCTCAAATATAGTTGTTGCTACTTCAATGATGAGTCAAGCACATTGGAGGATGCTGAGAAAGCAATGTTGGAGCTCTACTGTGAAAGGTCACAGATAAGAGATGGTCACACTGTTCTTGACGTTGGGTGTGGCTGGGGATCTTTGTCTTTGTACATTGCGCAGAAATACAACAACTGCAAGGTTACCGGGATCTGCAACTCAACGACACAAAAAGCTTTTATAGAAGAACAATGCCGGAATCTTCAGCTGCAGAATGTGGAGATCATCGTCGGAGATATCAGCACGTTTGAAATGGAGGCTTCCTTTGACCGAATATTTTCAATTGAAATGTTTGAGCATATGAAGAACTATAAGGATCTTCTGAAGAAGATATCCGGGTGGATGAAAGACGACGGCCTTCTGTTTGTTCATCATTTCTGCCATAAAGCATTTGCTTACCACTTTGAGGATAAAAGTGAAGATGACTGGATTACTAGGTACTTCTTCAGCGGGGGTACAATGCCCTCAGCAAATCTGCTACTTTATTTCCAGGATGATGTTTCCATTGTGAATCATTGGCTCGTCAACGGGAAGCACTACGCGCAAACAAGTGAAGAGTGGCTCAAAAGAATGGATCGGAACACGGCTTCCATAAAACCGATCATGGAGTCAACTTACGGCAAGGATTCAGCCGTGAAGTGGACGGTTTATTGGCGAACATTCTTCATTTCCGTTGCAGAACTCTTCGGGTACAACAACGGAGAAGAATGGATGGTTGTTCATTTCCTATTCAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

356

Amino Acids

41.36

Weight (kDa)

6.38

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 78 - 312 1e-46 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 125 - 236 8.7e-12 Methyltransferase domain
TRM5-TYW2_MTfase PF02475 126 - 238 5.7e-06 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 128 - 248 2.8e-16 Methyltransferase domain
MTS PF05175 129 - 202 5.7e-07 Methyltransferase small domain
GidB PF02527 129 - 202 7.9e-06 rRNA small subunit methyltransferase G
Methyltransf_25 PF13649 133 - 227 4.5e-16 Methyltransferase domain
Methyltransf_11 PF08241 134 - 231 3.3e-15 Methyltransferase domain
Methyltransf_12 PF08242 134 - 229 5.5e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 64
AccII CGCG 2 cut(s) 47, 866
AciI CCGC 3 cut(s) 64, 94, 774
AclWI GGATC 5 cut(s) 218, 430, 482, 648, 906
AcuI CTGAAG 3 cut(s) 512, 668, 754
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 5 cut(s) 26, 437, 764, 781, 1016
AfiI CCNNNNNNNGG 4 cut(s) 90, 138, 730, 963
AgsI TTSAA 8 cut(s) 176, 193, 265, 310, 575, 606, 611, 1057
AjnI CCWGG 1 cut(s) 813
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AloI GAACNNNNNNTCC 2 cut(s) 896, 928
AluBI AGCT 6 cut(s) 154, 160, 233, 358, 500, 532
AluI AGCT 6 cut(s) 154, 160, 233, 358, 500, 532
Alw21I GWGCWC 2 cut(s) 156, 360
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 5 cut(s) 218, 430, 482, 648, 906
AoxI GGCC 2 cut(s) 140, 679
ApeKI GCWGC 3 cut(s) 73, 106, 532
Asp700I GAANNNNTTC 1 cut(s) 601
AspLEI GCGC 3 cut(s) 49, 445, 868
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 2 cut(s) 472, 661
AvaII GGWCC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 156, 360
Bbv12I GWGCWC 2 cut(s) 156, 360
BbvCI CCTCAGC 1 cut(s) 790
BbvI GCAGC 3 cut(s) 60, 93, 519
BccI CCATC 3 cut(s) 262, 380, 1030
BceAI ACGGC 7 cut(s) 68, 105, 119, 694, 924, 941, 958
BciT130I CCWGG 1 cut(s) 815
BcnI CCSGG 2 cut(s) 472, 661
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 759
BfmI CTRYAG 1 cut(s) 533
BisI GCNGC 4 cut(s) 74, 95, 107, 533
BlsI GCNGC 4 cut(s) 75, 96, 108, 534
Bme1390I CCNGG 3 cut(s) 472, 661, 815
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 3 cut(s) 472, 661, 815
BmsI GCATC 3 cut(s) 19, 189, 325
BpmI CTGGAG 2 cut(s) 80, 140
Bpu10I CCTNAGC 1 cut(s) 790
BpuMI CCSGG 2 cut(s) 472, 661
BsaHI GRCGYC 1 cut(s) 81
BsaWI WCCGGW 3 cut(s) 39, 126, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 138, 730, 963
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 2 cut(s) 354, 438
BseBI CCWGG 1 cut(s) 815
BseGI GGATG 4 cut(s) 340, 672, 823, 1041
BseLI CCNNNNNNNGG 4 cut(s) 90, 138, 730, 963
BseMI GCAATG 2 cut(s) 354, 438
BseMII CTCAG 2 cut(s) 330, 804
BseNI ACTGG 1 cut(s) 755
BseXI GCAGC 3 cut(s) 60, 93, 519
BseYI CCCAGC 1 cut(s) 417
Bsh1236I CGCG 2 cut(s) 47, 866
BshFI GGCC 2 cut(s) 142, 681
BsiHKAI GWGCWC 2 cut(s) 156, 360
BsiSI CCGG 7 cut(s) 40, 77, 127, 132, 471, 520, 660
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 4 cut(s) 90, 138, 730, 963
BsmAI GTCTC 1 cut(s) 128
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 142, 681
Bsp1286I GDGCHC 2 cut(s) 156, 360
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
BspACI CCGC 3 cut(s) 64, 94, 774
BspANI GGCC 2 cut(s) 142, 681
BspCNI CTCAG 2 cut(s) 331, 803
BspFNI CGCG 2 cut(s) 47, 866
BspMAI CTGCAG 1 cut(s) 537
BspPI GGATC 5 cut(s) 218, 430, 482, 648, 906
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 2 cut(s) 354, 438
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 755
BssMI GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 5 cut(s) 24, 37, 365, 397, 970
Bst6I CTCTTC 2 cut(s) 873, 1013
BstACI GRCGYC 1 cut(s) 81
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 18, 51, 118
BstDEI CTNAG 2 cut(s) 339, 790
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 4 cut(s) 340, 672, 823, 1041
BstFNI CGCG 2 cut(s) 47, 866
BstHHI GCGC 3 cut(s) 49, 445, 868
BstKTI GATC 7 cut(s) 226, 425, 477, 549, 643, 901, 927
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
BstMWI GCNNNNNNNGC 5 cut(s) 55, 70, 103, 344, 865
BstNI CCWGG 1 cut(s) 815
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 3 cut(s) 470, 659, 813
BstSFI CTRYAG 1 cut(s) 533
BstUI CGCG 2 cut(s) 47, 866
BstV1I GCAGC 3 cut(s) 60, 93, 519
BstX2I RGATCY 3 cut(s) 422, 474, 640
BstYI RGATCY 3 cut(s) 422, 474, 640
BsuRI GGCC 2 cut(s) 142, 681
BtsCI GGATG 4 cut(s) 340, 672, 823, 1041
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 3 cut(s) 18, 51, 118
CfoI GCGC 3 cut(s) 49, 445, 868
Cfr13I GGNCC 1 cut(s) 129
CpoI CGGWCCG 1 cut(s) 129
CseI GACGC 1 cut(s) 89
Csp6I GTAC 5 cut(s) 25, 436, 763, 780, 1015
CspI CGGWCCG 1 cut(s) 129
CviAII CATG 1 cut(s) 928
CviQI GTAC 5 cut(s) 25, 436, 763, 780, 1015
DdeI CTNAG 2 cut(s) 339, 790
DpnI GATC 7 cut(s) 225, 424, 476, 548, 642, 900, 926
DpnII GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
Eam1104I CTCTTC 2 cut(s) 873, 1013
EarI CTCTTC 2 cut(s) 873, 1013
Ecl136II GAGCTC 2 cut(s) 154, 358
Eco147I AGGCCT 1 cut(s) 142
Eco24I GRGCYC 2 cut(s) 156, 360
Eco32I GATATC 2 cut(s) 562, 657
Eco47I GGWCC 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 154, 358
Eco57I CTGAAG 3 cut(s) 512, 668, 754
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 2 cut(s) 154, 358
EcoO65I GGTNACC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 813
EcoRV GATATC 2 cut(s) 562, 657
EcoT38I GRGCYC 2 cut(s) 156, 360
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 1 cut(s) 931
FatI CATG 1 cut(s) 927
FauI CCCGC 1 cut(s) 767
FauNDI CATATG 1 cut(s) 624
Fnu4HI GCNGC 4 cut(s) 74, 95, 107, 533
FokI GGATG 4 cut(s) 347, 679, 830, 1048
FriOI GRGCYC 2 cut(s) 156, 360
Fsp4HI GCNGC 4 cut(s) 74, 95, 107, 533
FspBI CTAG 1 cut(s) 759
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 3 cut(s) 48, 444, 867
GluI GCNGC 4 cut(s) 74, 95, 107, 533
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 2 cut(s) 80, 140
HaeIII GGCC 2 cut(s) 142, 681
HapII CCGG 7 cut(s) 40, 77, 127, 132, 471, 520, 660
HgaI GACGC 1 cut(s) 89
HhaI GCGC 3 cut(s) 49, 445, 868
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 1 cut(s) 931
Hin6I GCGC 3 cut(s) 47, 443, 866
HinP1I GCGC 3 cut(s) 47, 443, 866
HincII GTYRAC 2 cut(s) 850, 936
HindII GTYRAC 2 cut(s) 850, 936
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 6 cut(s) 67, 317, 523, 835, 932, 950
HpaII CCGG 7 cut(s) 40, 77, 127, 132, 471, 520, 660
Hpy166II GTNNAC 3 cut(s) 850, 936, 966
Hpy188I TCNGA 5 cut(s) 151, 166, 557, 648, 903
Hpy188III TCNNGA 4 cut(s) 88, 265, 401, 1057
Hpy8I GTNNAC 3 cut(s) 850, 936, 966
Hpy99I CGWCG 3 cut(s) 89, 557, 680
HpyAV CCTTC 4 cut(s) 153, 182, 187, 692
HpyCH4III ACNGT 5 cut(s) 24, 37, 365, 397, 970
HpyCH4IV ACGT 2 cut(s) 405, 569
HpyCH4V TGCA 6 cut(s) 16, 184, 462, 480, 535, 1001
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 70, 103, 344, 865
HpyF3I CTNAG 2 cut(s) 339, 790
HpySE526I ACGT 2 cut(s) 405, 569
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 1 cut(s) 931
HspAI GCGC 3 cut(s) 47, 443, 866
Kzo9I GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
LmnI GCTCC 3 cut(s) 61, 159, 355
Lsp1109I GCAGC 3 cut(s) 60, 93, 519
LweI GCATC 3 cut(s) 19, 189, 325
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 405, 569
MaeIII GTNAC 4 cut(s) 134, 372, 389, 466
MalI GATC 7 cut(s) 225, 424, 476, 548, 642, 900, 926
MbiI CCGCTC 1 cut(s) 64
MboI GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 3 cut(s) 422, 474, 640
MhlI GDGCHC 2 cut(s) 156, 360
MluCI AATT 2 cut(s) 176, 606
MlyI GAGTC 2 cut(s) 326, 941
MmeI TCCRAC 3 cut(s) 189, 333, 535
MnlI CCTC 7 cut(s) 84, 157, 179, 325, 574, 724, 799
MroXI GAANNNNTTC 1 cut(s) 601
MspA1I CMGCKG 2 cut(s) 532, 774
MspI CCGG 7 cut(s) 40, 77, 127, 132, 471, 520, 660
MspR9I CCNGG 3 cut(s) 472, 661, 815
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 2 cut(s) 47, 866
MwoI GCNNNNNNNGC 5 cut(s) 55, 70, 103, 344, 865
NciI CCSGG 2 cut(s) 472, 661
NdeI CATATG 1 cut(s) 624
NdeII GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
NlaIII CATG 1 cut(s) 931
NmuCI GTSAC 2 cut(s) 372, 389
NsbI TGCGCA 1 cut(s) 444
PceI AGGCCT 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 67, 523, 835, 950
Pfl23II CGTACG 1 cut(s) 24
PflFI GACNNNGTC 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 4 cut(s) 75, 96, 108, 534
PleI GAGTC 2 cut(s) 325, 940
PpsI GAGTC 2 cut(s) 325, 940
Psp124BI GAGCTC 2 cut(s) 156, 360
Psp6I CCWGG 1 cut(s) 813
PspEI GGTNACC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 1 cut(s) 813
PspLI CGTACG 1 cut(s) 24
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 537
PsuI RGATCY 3 cut(s) 422, 474, 640
PsyI GACNNNGTC 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 5 cut(s) 26, 437, 764, 781, 1016
RsaNI GTAC 5 cut(s) 25, 436, 763, 780, 1015
Rsr2I CGGWCCG 1 cut(s) 129
RsrII CGGWCCG 1 cut(s) 129
SacI GAGCTC 2 cut(s) 156, 360
SatI GCNGC 4 cut(s) 74, 95, 107, 533
Sau3AI GATC 7 cut(s) 223, 422, 474, 546, 640, 898, 924
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 2 cut(s) 326, 941
ScrFI CCNGG 3 cut(s) 472, 661, 815
SduI GDGCHC 2 cut(s) 156, 360
SfaNI GCATC 3 cut(s) 19, 189, 325
SfcI CTRYAG 1 cut(s) 533
SinI GGWCC 1 cut(s) 129
Sse9I AATT 2 cut(s) 176, 606
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 3 cut(s) 64, 94, 774
SspI AATATT 1 cut(s) 600
SspMI CTAG 1 cut(s) 759
SstI GAGCTC 2 cut(s) 156, 360
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 3 cut(s) 470, 659, 813
TaaI ACNGT 5 cut(s) 24, 37, 365, 397, 970
TaiI ACGT 2 cut(s) 408, 572
TaqII GACCGA 1 cut(s) 609
TasI AATT 2 cut(s) 176, 606
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 4 cut(s) 67, 523, 835, 950
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 372, 389
TseI GCWGC 3 cut(s) 73, 106, 532
Tsp45I GTSAC 2 cut(s) 372, 389
TspDTI ATGAA 7 cut(s) 17, 258, 641, 683, 683, 979, 1034
TspGWI ACGGA 2 cut(s) 985, 1038
TspRI CASTG 1 cut(s) 400
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.