Rroxscaffold_7G00157650

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010879
Physical Location & Seq
Forward (+)
1398415 .. 1400266
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157650.1

Sequence Viewer

Length: 1068 bp
ATGGATCGAGTAATGCAGGCACCGTACGATGCTACAGTTCGATTCGCGCTGGCGTCACTAGAGAGGAATCTGTTACCTGACGCCGTTATAAGGCGGTTCACGCGCTTGCTCTTAGCGAGTCGGCTACGCTCCGGTTACCAGCCTTCCTCTGAGCTCCAGCTCTCCCAACTCCTCCAGTTCGTTAAGTCTCTAAAAGAGATGCCAATAGCTATCAAGACTGATGACGCTAAAGCTCAACACTATGAAGTACCCACTTCTTTCTTCAAGATGGTGCTTGGGAACAATCTCAAATATAGTTGCTGTTACTTCACTGATAAGTCAAGCAGCTTGGAGGATGCGGAGAAAGCCATGTTGGAGACGTACTGTGAAAGATCGCAGGTTAAAGATGGCCATACTGTTCTTGATGTTGGATGTGGATGGGGATCACTATCTCTGTACATTGCACAGAAATATAAGAATTGCAGGGTTACTGGGATCTGCAATTCAGCAACACAGAAGGCTTTTATAGAAGAGCAGTGTAGGAATCGTCAGCTGGAGAATGTGGAGATCATTGTTGCAGATATCAGCACATTTGAGATGGAGGCATCATATGACAGAATATTTTCCATCGAAATGTTCGAGCACATGAAGAACTACAAGGATCTTCTGAAGAAGATATCCCGGTGGATGAAACAGGACGGCCTTCTGTTTGTACATCATTTCTGTCACAAAGCATTTGCTTACCACTTTGAGGACAAAAGTGATGATGACTGGATTACTAGGTACTTCTTCAGTGGAGGTACAATGCCTTCAGCAAATCTCCTATTGTATTTCCAGGATGATGTTTCTGTTGTCGATCATTGGCTTGTCAATGGGAAGCATTATGCACAAACAAGTGAAGAATGGCTGAAAAGAATGGACCAGAATTCGTCTTCCATAAAACCAATTATGGAGTCAACTTATGGCAAGGATTCGGCTGTGAAGTGGACTGTGTATTGGAGAACTTTCTTTATTGCGGTGGCAGAACTCTTTGGCTACAACAACGGAGAAGAATGGATGGTTGCTCTCTTCCTATTCAAAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

355

Amino Acids

41.35

Weight (kDa)

6.76

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 75 - 312 1.1e-46 Mycolic acid cyclopropane synthetase
TRM5-TYW2_MTfase PF02475 125 - 238 3.8e-07 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 128 - 248 1.8e-16 Methyltransferase domain
Methyltransf_23 PF13489 128 - 235 1.6e-12 Methyltransferase domain
MTS PF05175 128 - 221 7.4e-08 Methyltransferase small domain
TehB PF03848 128 - 224 6.4e-07 Tellurite resistance protein TehB
Methyltransf_25 PF13649 133 - 227 3.3e-17 Methyltransferase domain
Methyltransf_11 PF08241 134 - 231 1.3e-16 Methyltransferase domain
Methyltransf_12 PF08242 134 - 229 7.8e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 2 cut(s) 47, 103
AciI CCGC 3 cut(s) 94, 338, 995
AclWI GGATC 4 cut(s) 12, 430, 482, 648
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 3 cut(s) 668, 754, 774
AcyI GRCGYC 2 cut(s) 53, 81
AfaI GTAC 7 cut(s) 26, 249, 362, 437, 693, 764, 781
AfiI CCNNNNNNNGG 2 cut(s) 90, 730
AgsI TTSAA 2 cut(s) 265, 1057
AjnI CCWGG 1 cut(s) 813
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 6 cut(s) 154, 160, 209, 233, 327, 532
AluI AGCT 6 cut(s) 154, 160, 209, 233, 327, 532
Alw21I GWGCWC 2 cut(s) 156, 624
Alw26I GTCTC 2 cut(s) 192, 350
AlwI GGATC 4 cut(s) 12, 430, 482, 648
AoxI GGCC 2 cut(s) 388, 679
ApeKI GCWGC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 904
Asp700I GAANNNNTTC 1 cut(s) 601
AspLEI GCGC 2 cut(s) 49, 105
AspS9I GGNCC 1 cut(s) 898
AsuC2I CCSGG 1 cut(s) 661
AvaII GGWCC 1 cut(s) 898
BalI TGGCCA 1 cut(s) 390
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 156
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
BbsI GAAGAC 1 cut(s) 903
Bbv12I GWGCWC 2 cut(s) 156, 624
BbvI GCAGC 1 cut(s) 336
BccI CCATC 6 cut(s) 262, 380, 411, 571, 614, 1030
BceAI ACGGC 2 cut(s) 68, 694
BciT130I CCWGG 1 cut(s) 815
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 192, 350
BfaI CTAG 2 cut(s) 59, 759
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 1 cut(s) 325
BlsI GCNGC 1 cut(s) 326
Bme1390I CCNGG 2 cut(s) 661, 815
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 2 cut(s) 661, 815
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 4 cut(s) 19, 189, 325, 593
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 903
BpmI CTGGAG 3 cut(s) 140, 158, 554
BpuMI CCSGG 1 cut(s) 661
BsaBI GATNNNNATC 2 cut(s) 421, 427
BsaHI GRCGYC 2 cut(s) 53, 81
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 730
Bse1I ACTGG 3 cut(s) 175, 475, 755
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 2 cut(s) 421, 427
BseBI CCWGG 1 cut(s) 815
BseGI GGATG 6 cut(s) 340, 416, 422, 672, 823, 1041
BseJI GATNNNNATC 2 cut(s) 421, 427
BseLI CCNNNNNNNGG 2 cut(s) 90, 730
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 3 cut(s) 175, 475, 755
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 336
Bsh1236I CGCG 2 cut(s) 47, 103
BshFI GGCC 2 cut(s) 390, 681
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 156, 624
BsiSI CCGG 2 cut(s) 132, 661
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 90, 730
BsmAI GTCTC 2 cut(s) 192, 350
BsmBI CGTCTC 1 cut(s) 350
BsnI GGCC 2 cut(s) 390, 681
Bsp1286I GDGCHC 2 cut(s) 156, 624
Bsp1407I TGTACA 2 cut(s) 435, 691
Bsp143I GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
BspACI CCGC 3 cut(s) 94, 338, 995
BspANI GGCC 2 cut(s) 390, 681
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 2 cut(s) 47, 103
BspLI GGNNCC 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 4 cut(s) 12, 430, 482, 648
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 438
BsrGI TGTACA 2 cut(s) 435, 691
BsrI ACTGG 3 cut(s) 175, 475, 755
BssMI GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
BssNI GRCGYC 2 cut(s) 53, 81
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 5 cut(s) 24, 37, 365, 397, 970
Bst6I CTCTTC 2 cut(s) 504, 1052
BstACI GRCGYC 2 cut(s) 53, 81
BstAUI TGTACA 2 cut(s) 435, 691
BstC8I GCNNGC 3 cut(s) 18, 51, 107
BstDEI CTNAG 2 cut(s) 112, 150
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 6 cut(s) 340, 416, 422, 672, 823, 1041
BstFNI CGCG 2 cut(s) 47, 103
BstHHI GCGC 2 cut(s) 49, 105
BstKTI GATC 7 cut(s) 7, 374, 425, 477, 549, 643, 838
BstMAI GTCTC 2 cut(s) 192, 350
BstMBI GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
BstMWI GCNNNNNNNGC 2 cut(s) 100, 344
BstNI CCWGG 1 cut(s) 815
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 659, 813
BstSFI CTRYAG 1 cut(s) 33
BstUI CGCG 2 cut(s) 47, 103
BstV1I GCAGC 1 cut(s) 336
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 2 cut(s) 474, 640
BstYI RGATCY 2 cut(s) 474, 640
BsuRI GGCC 2 cut(s) 390, 681
BtsCI GGATG 6 cut(s) 340, 416, 422, 672, 823, 1041
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 3 cut(s) 309, 521, 778
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 3 cut(s) 18, 51, 107
CfoI GCGC 2 cut(s) 49, 105
Cfr13I GGNCC 1 cut(s) 898
CseI GACGC 3 cut(s) 42, 89, 233
Csp6I GTAC 7 cut(s) 25, 248, 361, 436, 692, 763, 780
CviAII CATG 2 cut(s) 349, 625
CviQI GTAC 7 cut(s) 25, 248, 361, 436, 692, 763, 780
DdeI CTNAG 2 cut(s) 112, 150
DpnI GATC 7 cut(s) 6, 373, 424, 476, 548, 642, 837
DpnII GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
EaeI YGGCCR 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 504, 1052
EarI CTCTTC 2 cut(s) 504, 1052
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco32I GATATC 2 cut(s) 562, 657
Eco47I GGWCC 1 cut(s) 898
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 3 cut(s) 668, 754, 774
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 1 cut(s) 813
EcoRV GATATC 2 cut(s) 562, 657
EcoT38I GRGCYC 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 352, 628
FatI CATG 2 cut(s) 348, 624
FauNDI CATATG 1 cut(s) 589
Fnu4HI GCNGC 1 cut(s) 325
FokI GGATG 6 cut(s) 347, 423, 429, 679, 830, 1048
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 1 cut(s) 325
FspBI CTAG 2 cut(s) 59, 759
GlaI GCGC 2 cut(s) 48, 104
GluI GCNGC 1 cut(s) 325
GsuI CTGGAG 3 cut(s) 140, 158, 554
HaeIII GGCC 2 cut(s) 390, 681
HapII CCGG 2 cut(s) 132, 661
HgaI GACGC 3 cut(s) 42, 89, 233
HhaI GCGC 2 cut(s) 49, 105
Hin1I GRCGYC 2 cut(s) 53, 81
Hin1II CATG 2 cut(s) 352, 628
Hin6I GCGC 2 cut(s) 47, 103
HinP1I GCGC 2 cut(s) 47, 103
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HinfI GANTC 6 cut(s) 42, 67, 118, 523, 932, 950
HpaII CCGG 2 cut(s) 132, 661
Hpy166II GTNNAC 3 cut(s) 99, 936, 966
Hpy188I TCNGA 2 cut(s) 151, 648
Hpy188III TCNNGA 3 cut(s) 214, 265, 401
Hpy8I GTNNAC 3 cut(s) 99, 936, 966
HpyAV CCTTC 4 cut(s) 153, 490, 692, 798
HpyCH4III ACNGT 5 cut(s) 24, 37, 365, 397, 970
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 6 cut(s) 16, 443, 462, 480, 557, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 344
HpyF3I CTNAG 2 cut(s) 112, 150
HpySE526I ACGT 1 cut(s) 359
Hsp92I GRCGYC 2 cut(s) 53, 81
Hsp92II CATG 2 cut(s) 352, 628
HspAI GCGC 2 cut(s) 47, 103
Kzo9I GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 2 cut(s) 134, 159
Lsp1109I GCAGC 1 cut(s) 336
LweI GCATC 4 cut(s) 19, 189, 325, 593
MaeI CTAG 2 cut(s) 59, 759
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 6 cut(s) 54, 72, 134, 302, 466, 704
MalI GATC 7 cut(s) 6, 373, 424, 476, 548, 642, 837
MboI GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
MflI RGATCY 2 cut(s) 474, 640
MhlI GDGCHC 2 cut(s) 156, 624
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 4 cut(s) 457, 481, 904, 924
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 2 cut(s) 127, 941
MmeI TCCRAC 2 cut(s) 333, 388
MnlI CCTC 7 cut(s) 57, 157, 182, 325, 574, 724, 770
Mox20I TGGCCA 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 601
MscI TGGCCA 1 cut(s) 390
MseI TTAA 2 cut(s) 183, 381
MslI CAYNNNNRTG 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 2 cut(s) 132, 661
MspR9I CCNGG 2 cut(s) 661, 815
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 2 cut(s) 47, 103
MwoI GCNNNNNNNGC 2 cut(s) 100, 344
NciI CCSGG 1 cut(s) 661
NdeI CATATG 1 cut(s) 589
NdeII GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
NlaIII CATG 2 cut(s) 352, 628
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 54, 704
PciSI GCTCTTC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 42, 67, 523, 950
Pfl23II CGTACG 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 1 cut(s) 326
PleI GAGTC 2 cut(s) 126, 940
PpsI GAGTC 2 cut(s) 126, 940
PsiI TTATAA 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 813
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 813
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 898
PsuI RGATCY 2 cut(s) 474, 640
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 7 cut(s) 26, 249, 362, 437, 693, 764, 781
RsaNI GTAC 7 cut(s) 25, 248, 361, 436, 692, 763, 780
RseI CAYNNNNRTG 1 cut(s) 611
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 2 cut(s) 183, 381
SatI GCNGC 1 cut(s) 325
Sau3AI GATC 7 cut(s) 4, 371, 422, 474, 546, 640, 835
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 2 cut(s) 127, 941
ScrFI CCNGG 2 cut(s) 661, 815
SduI GDGCHC 2 cut(s) 156, 624
SfaNI GCATC 4 cut(s) 19, 189, 325, 593
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 1 cut(s) 611
Sse9I AATT 4 cut(s) 457, 481, 904, 924
SsiI CCGC 3 cut(s) 94, 338, 995
SspI AATATT 1 cut(s) 600
SspMI CTAG 2 cut(s) 59, 759
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 659, 813
TaaI ACNGT 5 cut(s) 24, 37, 365, 397, 970
TaiI ACGT 1 cut(s) 362
TaqI TCGA 5 cut(s) 7, 40, 609, 618, 834
TasI AATT 4 cut(s) 457, 481, 904, 924
TatI WGTACW 2 cut(s) 435, 691
TfiI GAWTC 4 cut(s) 42, 67, 523, 950
Tru1I TTAA 2 cut(s) 183, 381
Tru9I TTAA 2 cut(s) 183, 381
TscAI CASTG 3 cut(s) 316, 521, 778
TseFI GTSAC 2 cut(s) 54, 704
TseI GCWGC 1 cut(s) 324
Tsp45I GTSAC 2 cut(s) 54, 704
TspDTI ATGAA 3 cut(s) 258, 641, 683
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 3 cut(s) 316, 521, 778
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 2 cut(s) 59, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.