Rw7G029480

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010879
Physical Location & Seq
Reverse (-)
40892616 .. 40893790
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G029480.1

Sequence Viewer

Length: 555 bp
ATGGTCACTGTTCTTGATGTTGGATGTGGATGGGGATCACCATCTCTGTACATTGCACTGAAATATAAGAATTGCAAGGTTACTGGGATCTGCATTTCAGCAACAGATAAGGCTTTTATAGAAGAGCAATGTAGGAATCATCAGCTGGAGAATGTGAAGATCATTATTGCAGATATCAGCACATTTGAGGTAGAGGCATCATATGACAGAATATTTTCCATTGAAATGTTTGAGTTTGTACATCATTTCTGTCACAAAGCATTTGCTTACCACTTTGAGATTACTAGGCACTTCTTCAGGGGAGGTACAATGCTTTCAGCAAATCTACTACTGTATTTCCAGGATGATGTTTCTGTTGTCGATCATTGGCTTGTCAATGGGAAGCATTATAATTCGGCTTCCATAAAACCAATTATGGAGTCAACTTATGGCAAGGATTCGGCTGTGAAGTGGACTGTGTATTGGAGAACTTTCTTTATTGCAGTTGCAGAACTCTTTGGCTACAACAACGGAGAAGAATGGATGATTGCTCTCTTCCTATTCAAAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

184

Amino Acids

21.34

Weight (kDa)

6.44

Isoelectric Point (pI)

22.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 3 - 117 2.9e-15 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 3 - 108 1.1e-10 Methyltransferase domain
TehB PF03848 3 - 82 4.1e-07 Tellurite resistance protein TehB
GidB PF02527 3 - 73 1e-06 rRNA small subunit methyltransferase G
Methyltransf_25 PF13649 4 - 88 4.5e-12 Methyltransferase domain
Methyltransf_11 PF08241 5 - 88 1.4e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AclWI GGATC 2 cut(s) 43, 95
AcuI CTGAAG 1 cut(s) 280
AfaI GTAC 3 cut(s) 50, 240, 307
AgsI TTSAA 2 cut(s) 224, 544
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
AlwI GGATC 2 cut(s) 43, 95
Asp700I GAANNNNTTC 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 30
BccI CCATC 2 cut(s) 24, 49
BciT130I CCWGG 1 cut(s) 341
BfaI CTAG 1 cut(s) 285
Bme1390I CCNGG 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 341
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 167
BsaBI GATNNNNATC 2 cut(s) 34, 40
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 2 cut(s) 51, 134
Bse8I GATNNNNATC 2 cut(s) 34, 40
BseBI CCWGG 1 cut(s) 341
BseGI GGATG 4 cut(s) 29, 35, 349, 528
BseJI GATNNNNATC 2 cut(s) 34, 40
BseMI GCAATG 2 cut(s) 51, 134
BseNI ACTGG 1 cut(s) 88
Bsp1407I TGTACA 2 cut(s) 48, 238
Bsp143I GATC 4 cut(s) 35, 87, 159, 361
BspPI GGATC 2 cut(s) 43, 95
BspQI GCTCTTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 51, 134
BsrGI TGTACA 2 cut(s) 48, 238
BsrI ACTGG 1 cut(s) 88
BssMI GATC 4 cut(s) 35, 87, 159, 361
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 3 cut(s) 10, 333, 457
Bst6I CTCTTC 2 cut(s) 117, 539
BstAUI TGTACA 2 cut(s) 48, 238
BstF5I GGATG 4 cut(s) 29, 35, 349, 528
BstKTI GATC 4 cut(s) 38, 90, 162, 364
BstMBI GATC 4 cut(s) 35, 87, 159, 361
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BtsCI GGATG 4 cut(s) 29, 35, 349, 528
BtsIMutI CAGTG 2 cut(s) 6, 56
Csp6I GTAC 3 cut(s) 49, 239, 306
CviJI RGCY 6 cut(s) 113, 145, 370, 398, 443, 501
CviKI_1 RGCY 6 cut(s) 113, 145, 370, 398, 443, 501
CviQI GTAC 3 cut(s) 49, 239, 306
DpnI GATC 4 cut(s) 37, 89, 161, 363
DpnII GATC 4 cut(s) 35, 87, 159, 361
Eam1104I CTCTTC 2 cut(s) 117, 539
EarI CTCTTC 2 cut(s) 117, 539
Eco32I GATATC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 339
EcoRV GATATC 1 cut(s) 175
FaiI YATR 8 cut(s) 66, 119, 202, 204, 390, 404, 416, 429
FauNDI CATATG 1 cut(s) 202
FokI GGATG 4 cut(s) 36, 42, 356, 535
FspBI CTAG 1 cut(s) 285
GsuI CTGGAG 1 cut(s) 167
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HinfI GANTC 3 cut(s) 136, 419, 437
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 2 cut(s) 423, 453
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 2 cut(s) 423, 453
HpyCH4III ACNGT 3 cut(s) 10, 333, 457
HpyCH4V TGCA 6 cut(s) 56, 75, 93, 170, 482, 488
Kzo9I GATC 4 cut(s) 35, 87, 159, 361
LguI GCTCTTC 1 cut(s) 117
LpnPI CCDG 5 cut(s) 69, 131, 283, 326, 353
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 4, 79, 251
MalI GATC 4 cut(s) 37, 89, 161, 363
MboI GATC 4 cut(s) 35, 87, 159, 361
MboII GAAGA 5 cut(s) 134, 169, 286, 526, 527
MflI RGATCY 1 cut(s) 87
MluCI AATT 3 cut(s) 70, 391, 411
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 3 cut(s) 181, 187, 296
MroXI GAANNNNTTC 1 cut(s) 214
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 341
MvaI CCWGG 1 cut(s) 341
NdeI CATATG 1 cut(s) 202
NdeII GATC 4 cut(s) 35, 87, 159, 361
NmuCI GTSAC 2 cut(s) 4, 251
PciSI GCTCTTC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 136, 437
PfoI TCCNGGA 1 cut(s) 339
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PsiI TTATAA 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PsuI RGATCY 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 3 cut(s) 50, 240, 307
RsaNI GTAC 3 cut(s) 49, 239, 306
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 117
Sau3AI GATC 4 cut(s) 35, 87, 159, 361
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 341
SetI ASST 4 cut(s) 81, 147, 192, 307
SfaNI GCATC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 224
Sse9I AATT 3 cut(s) 70, 391, 411
SspI AATATT 1 cut(s) 213
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 339
TaaI ACNGT 3 cut(s) 10, 333, 457
TaqI TCGA 1 cut(s) 360
TasI AATT 3 cut(s) 70, 391, 411
TatI WGTACW 2 cut(s) 48, 238
TfiI GAWTC 2 cut(s) 136, 437
TscAI CASTG 2 cut(s) 13, 63
TseFI GTSAC 2 cut(s) 4, 251
Tsp45I GTSAC 2 cut(s) 4, 251
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 2 cut(s) 13, 63
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.