Rh6BG513500

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010879
Physical Location & Seq
Reverse (-)
70872887 .. 70873736
850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG513500.1

Sequence Viewer

Length: 456 bp
ATGTTCGAGCACATGAAGAACTACAAGGATCTTCTGAAGAAGATATCCGGGTGGATGAAACAGGACGGCCTTCTGTTTGTACATCATTTCTGTCACAAAGCATTTGCTTACCACTTTGAGGACAAAAGTGATGATGACTGGATTACTAGGTACTTCTTCAGTGGAGGTACAATGCCTTCAGCAAATCTACTACTGTATTTCCAGGATGATGTTTCTGTTGTCGATCATTGGCTTGTCAATGGGAAACATTATGCACAAACAAGTGAAGAATGGCTGAAAAGAATGGATCAGAATTCAGCTTCCATAAAACCAATTATGGAGTCAACTTATGGCAAGGATTCGGCTGTGAAGTGGACTGTGTATTGGAGAACTTTCTTTATTGCAGTTGCAGAACTCTTTGGCTACAACAACGGAGAAGAATGGATGGTTGCTCTCTTCTTATTCAAAAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

151

Amino Acids

17.99

Weight (kDa)

6.65

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 1 - 108 4.3e-11 Mycolic acid cyclopropane synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 36, 294
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 3 cut(s) 56, 142, 162
AfaI GTAC 3 cut(s) 81, 152, 169
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 1 cut(s) 445
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw21I GWGCWC 1 cut(s) 12
AlwI GGATC 2 cut(s) 36, 294
AoxI GGCC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 49
BarI GAAGNNNNNNTAC 2 cut(s) 160, 192
Bbv12I GWGCWC 1 cut(s) 12
BccI CCATC 1 cut(s) 418
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 203
BcnI CCSGG 1 cut(s) 49
BfaI CTAG 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 49, 203
BmrFI CCNGG 2 cut(s) 49, 203
BpuMI CCSGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 143
BseBI CCWGG 1 cut(s) 203
BseGI GGATG 3 cut(s) 60, 211, 429
BseLI CCNNNNNNNGG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 143
BshFI GGCC 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 118
BsnI GGCC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 1 cut(s) 79
Bsp143I GATC 3 cut(s) 28, 223, 286
BspANI GGCC 1 cut(s) 69
BspPI GGATC 2 cut(s) 36, 294
BsrGI TGTACA 1 cut(s) 79
BsrI ACTGG 1 cut(s) 143
BssMI GATC 3 cut(s) 28, 223, 286
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 195, 358
Bst6I CTCTTC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 79
BstF5I GGATG 3 cut(s) 60, 211, 429
BstKTI GATC 3 cut(s) 31, 226, 289
BstMBI GATC 3 cut(s) 28, 223, 286
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 47, 201
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 1 cut(s) 69
BtsCI GGATG 3 cut(s) 60, 211, 429
BtsIMutI CAGTG 1 cut(s) 166
Csp6I GTAC 3 cut(s) 80, 151, 168
CviAII CATG 1 cut(s) 13
CviJI RGCY 6 cut(s) 69, 232, 274, 299, 344, 402
CviKI_1 RGCY 6 cut(s) 69, 232, 274, 299, 344, 402
CviQI GTAC 3 cut(s) 80, 151, 168
DpnI GATC 3 cut(s) 30, 225, 288
DpnII GATC 3 cut(s) 28, 223, 286
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 3 cut(s) 56, 142, 162
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 45
FaeI CATG 1 cut(s) 16
FaiI YATR 5 cut(s) 14, 252, 305, 317, 330
FatI CATG 1 cut(s) 12
FokI GGATG 3 cut(s) 67, 218, 436
FspBI CTAG 1 cut(s) 147
HaeIII GGCC 1 cut(s) 69
HapII CCGG 1 cut(s) 48
Hin1II CATG 1 cut(s) 16
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 2 cut(s) 320, 338
HpaII CCGG 1 cut(s) 48
Hpy166II GTNNAC 2 cut(s) 324, 354
Hpy188I TCNGA 2 cut(s) 36, 291
Hpy8I GTNNAC 2 cut(s) 324, 354
HpyAV CCTTC 2 cut(s) 80, 186
HpyCH4III ACNGT 2 cut(s) 195, 358
HpyCH4V TGCA 3 cut(s) 254, 383, 389
Hsp92II CATG 1 cut(s) 16
Kzo9I GATC 3 cut(s) 28, 223, 286
LpnPI CCDG 5 cut(s) 47, 61, 124, 188, 215
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 92
MalI GATC 3 cut(s) 30, 225, 288
MboI GATC 3 cut(s) 28, 223, 286
MboII GAAGA 8 cut(s) 23, 28, 49, 52, 148, 278, 427, 428
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 2 cut(s) 292, 312
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 2 cut(s) 112, 158
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 49, 203
MvaI CCWGG 1 cut(s) 203
NciI CCSGG 1 cut(s) 49
NdeII GATC 3 cut(s) 28, 223, 286
NlaIII CATG 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 338
PfoI TCCNGGA 1 cut(s) 201
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 3 cut(s) 81, 152, 169
RsaNI GTAC 3 cut(s) 80, 151, 168
Sau3AI GATC 3 cut(s) 28, 223, 286
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 49, 203
SduI GDGCHC 1 cut(s) 12
SetI ASST 3 cut(s) 152, 169, 301
Sse9I AATT 2 cut(s) 292, 312
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 47, 201
TaaI ACNGT 2 cut(s) 195, 358
TaqI TCGA 2 cut(s) 6, 222
TasI AATT 2 cut(s) 292, 312
TatI WGTACW 1 cut(s) 79
TfiI GAWTC 1 cut(s) 338
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 29, 71
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 1 cut(s) 166
XapI RAATTY 1 cut(s) 292
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.