Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009744

Physical Location & Seq

Genomic Coordinates Forward (+)

16022269 .. 16026428

Gene Structure 4160 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G33220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGTCATGTTTGAAGTATTTCTTGATCTTATTGATGTTGGGACTGTGTGTCTCATCGGAGGAGAATTTTCAATATGACTACTTGAAAGTTCCGGCAAGCGAGTTTGTGAGCTCCATAAACACAATTGTGGTAGTGATAAGACAAGTGAGTTCAATTTTGTCTCAGTTCGCTGATTTCAGCGGAGATCGCCGGCTGCAGAATGCGGTTTCCGATTGCCTTGATCTGCTTGACTTTTCTTCAGAGGAGCTCACTTGGTCTGCTTCTGCTTCTGAGAATCCAAAAGGTAAGGGCAACGGAACAGGGGACGTTGGATCGGACACAAGGACATGGTTAAGTGCTGCTCTCTCCAACCAAGCCACTTGTATGGAAGGCTTCGATGGTACCTCTGGTCTCGTAAAATCCCTTGTCGCTGGCAGTCTCGATCAACTATATTCTATGCTTAGGGAATTGTTACCACTTGTGCAACCAGAGCAGAAACCCAAGGCTGTTTCTAAACCTGGCCCTATTGCTAAAGGACCGAAGGCACCACCTGGCCGTAAACTCAGAGACACCGACGAAGACGAAAGCCTTCAATTCCCAGACTGGGTCAGACCGGACGATCGTAAACTATTAGAATCAAACGGTAGGACTTACGACGTTAGTGTAGCATTGGATGGGACAGGCAACTTCACAAAGATAATGGACGCCATAAAGAAAGCTCCTGATTATAGCTCGACACGTTTCGTCATATACATTAAAAAAGGTTTATATTTGGAGAATGTTGAGATCAAGAAGAAGAAATGGAACATTGTAATGTTAGGTGATGGCATTGACGTGACTGTTATTTCCGGTAACCGCAGCTTCATCGATGGTTGGACCACTTTCCGATCAGCTACATTCGCGGTAAGCGGAAGAGGATTCTTAGCAAGAGATATAACGTTCCAGAACACAGCAGGGCCAGAGAAGCATCAGGCGGTAGCACTGAGATCAGACTCTGACCTCTCTGTGTTCTTCAGATGTGCGATGAGGGGTTATCAAGATACACTCTACACGCACACCATGCGTCAGTTCTACCGTGAGTGCACCATCACGGGAACAGTAGATTTTATATTTGGGGATGGGACTGTTGTGTTTCAAAACTGTCAAATTTTGGCTAAAAGAGGACTCCCTAACCAAAAGAACACCATCACTGCACAAGGCCGGAAAGATGTCAACCAACCCTCAGGATTCTCAATTCAATTCAGCAATATCTCGGCGGACGCAGATTTGGTCCCATACCTAAACACGACAAGGACGTATCTAGGAAGGCCATGGAAGCTATATTCGAGAACGGTTTTCATTAGGAACAATATGAGCGACGTTGTGAGACCCGAAGGATGGCTTGAGTGGAACGCTGATTTTGCGTTGGACACACTCTTCTACGGAGAGTTCATGAACTACGGGCCTGGTTCAGGACTCAGCAGCCGAGTCAAATGGCCTGGTTACCACGTCTTTAACAACTCGGATCAGGCTAACAACTTCACCGTTTCTCAGTTCATTAAAGGAAATCTTTGGCTGCCTTCTACGGGAGTAACGTTTAGTGATGGCTTGTATATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

525

Amino Acids

58.5

Weight (kDa)

6.85

Isoelectric Point (pI)

30.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	44 - 142	5.6e-15	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	212 - 509	3.9e-147	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43270 AT4G33220 AT4G33220
fragaria_vesca	FvH4_1g09610
malus_domestica	MD02G1104600.v1.1 MD02G1105100.v1.1 MD15G1224900.v1.1
prunus_persica	Prupe.7G190300_v2.0.a1
pyrus_communis	pycom02g08260
rosa_chinensis	RchiOBHm_Chr2g0096461
rosa_laevigata	RLG00000016641
rosa_multiflora	Rmu_co8263641.1_g000001 Rmu_sc0004096.1_g000019 Rmu_sc0029740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00145620
rosa_rugosa	Rorug02G0057900
rosa_samantha	Rh2AG104800 Rh2BG107000 Rh2CG108800 Rh2DG108000
rosa_wichuraiana	Rw2G008100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	380
AccB1I	GGYRCC	2 cut(s)	380, 523
AccII	CGCG	1 cut(s)	881
AciI	CCGC	7 cut(s)	180, 203, 835, 881, 888, 953, 1235
AclI	AACGTT	2 cut(s)	917, 1553
AclWI	GGATC	2 cut(s)	319, 1491
AcoI	YGGCCR	1 cut(s)	532
AcsI	RAATTY	2 cut(s)	64, 1125
AcuI	CTGAAG	2 cut(s)	222, 976
AcyI	GRCGYC	1 cut(s)	684
AfaI	GTAC	1 cut(s)	382
AfiI	CCNNNNNNNGG	4 cut(s)	583, 1356, 1430, 1544
AflIII	ACRYGT	1 cut(s)	716
AgsI	TTSAA	7 cut(s)	13, 71, 85, 153, 572, 1115, 1217
AhdI	GACNNNNNGTC	1 cut(s)	47
AjiI	CACGTC	2 cut(s)	814, 1468
AjnI	CCWGG	4 cut(s)	496, 529, 1423, 1456
AleI	CACNNNNGTG	1 cut(s)	125
AluBI	AGCT	7 cut(s)	111, 247, 698, 711, 840, 872, 1297
AluI	AGCT	7 cut(s)	111, 247, 698, 711, 840, 872, 1297
Alw21I	GWGCWC	3 cut(s)	113, 249, 1064
Alw26I	GTCTC	6 cut(s)	55, 165, 395, 422, 540, 1339
Alw44I	GTGCAC	1 cut(s)	1060
AlwI	GGATC	2 cut(s)	319, 1491
AlwNI	CAGNNNCTG	1 cut(s)	974
AoxI	GGCC	7 cut(s)	499, 532, 935, 1177, 1286, 1421, 1454
ApaLI	GTGCAC	1 cut(s)	1060
ApeKI	GCWGC	5 cut(s)	193, 338, 837, 1440, 1534
ApoI	RAATTY	2 cut(s)	64, 1125
Asp700I	GAANNNNTTC	2 cut(s)	17, 567
Asp718I	GGTACC	1 cut(s)	380
AspS9I	GGNCC	6 cut(s)	500, 515, 855, 935, 1249, 1421
AsuHPI	GGTGA	2 cut(s)	812, 1492
AvaII	GGWCC	3 cut(s)	515, 855, 1249
AxyI	CCTNAGG	1 cut(s)	1201
BaeGI	GKGCMC	1 cut(s)	1064
BanI	GGYRCC	2 cut(s)	380, 523
BanII	GRGCYC	2 cut(s)	113, 249
BbsI	GAAGAC	1 cut(s)	564
Bbv12I	GWGCWC	3 cut(s)	113, 249, 1064
BbvI	GCAGC	5 cut(s)	180, 325, 849, 1452, 1521
BccI	CCATC	9 cut(s)	371, 647, 797, 842, 1073, 1091, 1172, 1350, 1556
BceAI	ACGGC	1 cut(s)	519
BcgI	CGANNNNNNTGC	2 cut(s)	826, 860
BciT130I	CCWGG	4 cut(s)	498, 531, 1425, 1458
BcoDI	GTCTC	6 cut(s)	55, 165, 395, 422, 540, 1339
BfaI	CTAG	1 cut(s)	1280
BfmI	CTRYAG	1 cut(s)	194
BisI	GCNGC	5 cut(s)	194, 339, 838, 1441, 1535
BlsI	GCNGC	5 cut(s)	195, 340, 839, 1442, 1536
Bme1390I	CCNGG	4 cut(s)	498, 531, 1425, 1458
Bme18I	GGWCC	3 cut(s)	515, 855, 1249
BmeRI	GACNNNNNGTC	1 cut(s)	47
BmgBI	CACGTC	2 cut(s)	814, 1468
BmgT120I	GGNCC	6 cut(s)	500, 515, 855, 935, 1249, 1421
BmiI	GGNNCC	3 cut(s)	382, 525, 1251
BmrFI	CCNGG	4 cut(s)	498, 531, 1425, 1458
BmrI	ACTGGG	1 cut(s)	592
BmsI	GCATC	1 cut(s)	955
BmuI	ACTGGG	1 cut(s)	592
BpiI	GAAGAC	1 cut(s)	564
Bpu10I	CCTNAGC	1 cut(s)	440
BpuEI	CTTGAG	1 cut(s)	1382
Bsa29I	ATCGAT	1 cut(s)	846
BsaHI	GRCGYC	1 cut(s)	684
BsaI	GGTCTC	2 cut(s)	395, 1339
BsaJI	CCNNGG	2 cut(s)	480, 1289
BsaWI	WCCGGW	2 cut(s)	592, 827
Bsc4I	CCNNNNNNNGG	4 cut(s)	583, 1356, 1430, 1544
Bse118I	RCCGGY	1 cut(s)	189
Bse1I	ACTGG	1 cut(s)	587
Bse21I	CCTNAGG	1 cut(s)	1201
BseBI	CCWGG	4 cut(s)	498, 531, 1425, 1458
BseCI	ATCGAT	1 cut(s)	846
BseDI	CCNNGG	2 cut(s)	480, 1289
BseGI	GGATG	3 cut(s)	658, 1102, 1361
BseLI	CCNNNNNNNGG	4 cut(s)	583, 1356, 1430, 1544
BseMII	CTCAG	7 cut(s)	176, 261, 556, 953, 1215, 1450, 1523
BseNI	ACTGG	1 cut(s)	587
BseRI	GAGGAG	2 cut(s)	74, 257
BseSI	GKGCMC	1 cut(s)	1064
BseXI	GCAGC	5 cut(s)	180, 325, 849, 1452, 1521
BsgI	GTGCAG	1 cut(s)	1155
Bsh1236I	CGCG	1 cut(s)	881
Bsh1285I	CGRYCG	1 cut(s)	601
BshFI	GGCC	7 cut(s)	501, 534, 937, 1179, 1288, 1423, 1456
BshNI	GGYRCC	2 cut(s)	380, 523
BshVI	ATCGAT	1 cut(s)	846
BsiEI	CGRYCG	1 cut(s)	601
BsiHKAI	GWGCWC	3 cut(s)	113, 249, 1064
BsiSI	CCGG	5 cut(s)	92, 190, 593, 828, 1180
BslFI	GGGAC	5 cut(s)	54, 317, 670, 1114, 1235
BslI	CCNNNNNNNGG	4 cut(s)	583, 1356, 1430, 1544
BsmAI	GTCTC	6 cut(s)	55, 165, 395, 422, 540, 1339
BsmFI	GGGAC	5 cut(s)	54, 317, 670, 1114, 1235
BsmI	GAATGC	1 cut(s)	205
BsnI	GGCC	7 cut(s)	501, 534, 937, 1179, 1288, 1423, 1456
Bso31I	GGTCTC	2 cut(s)	395, 1339
Bsp1286I	GDGCHC	3 cut(s)	113, 249, 1064
Bsp19I	CCATGG	1 cut(s)	1289
BspACI	CCGC	7 cut(s)	180, 203, 835, 881, 888, 953, 1235
BspANI	GGCC	7 cut(s)	501, 534, 937, 1179, 1288, 1423, 1456
BspCNI	CTCAG	7 cut(s)	175, 262, 555, 954, 1214, 1449, 1522
BspDI	ATCGAT	1 cut(s)	846
BspFNI	CGCG	1 cut(s)	881
BspHI	TCATGA	1 cut(s)	1410
BspLI	GGNNCC	3 cut(s)	382, 525, 1251
BspMAI	CTGCAG	1 cut(s)	198
BspPI	GGATC	2 cut(s)	319, 1491
BspT107I	GGYRCC	2 cut(s)	380, 523
BspTNI	GGTCTC	2 cut(s)	395, 1339
BsrFI	RCCGGY	1 cut(s)	189
BsrI	ACTGG	1 cut(s)	587
BssAI	RCCGGY	1 cut(s)	189
BssECI	CCNNGG	2 cut(s)	480, 1289
BssNI	GRCGYC	1 cut(s)	684
BssT1I	CCWWGG	2 cut(s)	480, 1289
Bst2UI	CCWGG	4 cut(s)	498, 531, 1425, 1458
Bst4CI	ACNGT	9 cut(s)	45, 623, 820, 1055, 1078, 1105, 1121, 1312, 1504
Bst6I	CTCTTC	2 cut(s)	886, 1400
BstACI	GRCGYC	1 cut(s)	684
BstAPI	GCANNNNNTGC	1 cut(s)	1039
BstC8I	GCNNGC	3 cut(s)	97, 191, 412
BstDEI	CTNAG	9 cut(s)	162, 270, 440, 542, 901, 962, 1201, 1436, 1509
BstDSI	CCRYGG	1 cut(s)	1289
BstEII	GGTNACC	2 cut(s)	830, 1460
BstENI	CCTNNNNNAGG	1 cut(s)	1428
BstF5I	GGATG	3 cut(s)	658, 1102, 1361
BstFNI	CGCG	1 cut(s)	881
BstMAI	GTCTC	6 cut(s)	55, 165, 395, 422, 540, 1339
BstMCI	CGRYCG	1 cut(s)	601
BstMWI	GCNNNNNNNGC	7 cut(s)	186, 469, 878, 943, 1039, 1294, 1379
BstNI	CCWGG	4 cut(s)	498, 531, 1425, 1458
BstPI	GGTNACC	2 cut(s)	830, 1460
BstSCI	CCNGG	4 cut(s)	496, 529, 1423, 1456
BstSFI	CTRYAG	1 cut(s)	194
BstSLI	GKGCMC	1 cut(s)	1064
BstUI	CGCG	1 cut(s)	881
BstV1I	GCAGC	5 cut(s)	180, 325, 849, 1452, 1521
BstV2I	GAAGAC	1 cut(s)	564
BstXI	CCANNNNNNTGG	1 cut(s)	364
Bsu15I	ATCGAT	1 cut(s)	846
Bsu36I	CCTNAGG	1 cut(s)	1201
BsuRI	GGCC	7 cut(s)	501, 534, 937, 1179, 1288, 1423, 1456
BsuTUI	ATCGAT	1 cut(s)	846
BtgI	CCRYGG	1 cut(s)	1289
BtgZI	GCGATG	1 cut(s)	1016
BtrI	CACGTC	2 cut(s)	814, 1468
BtsCI	GGATG	3 cut(s)	658, 1102, 1361
BtsI	GCAGTG	1 cut(s)	1167
BtsIMutI	CAGTG	2 cut(s)	959, 1167
Cac8I	GCNNGC	3 cut(s)	97, 191, 412
CaiI	CAGNNNCTG	1 cut(s)	974
CciI	TCATGA	1 cut(s)	1410
Cfr10I	RCCGGY	1 cut(s)	189
Cfr13I	GGNCC	6 cut(s)	500, 515, 855, 935, 1249, 1421
ClaI	ATCGAT	1 cut(s)	846
CseI	GACGC	3 cut(s)	692, 1031, 1247
Csp6I	GTAC	1 cut(s)	381
CviAII	CATG	5 cut(s)	6, 327, 1039, 1290, 1411
CviQI	GTAC	1 cut(s)	381
DdeI	CTNAG	9 cut(s)	162, 270, 440, 542, 901, 962, 1201, 1436, 1509
DriI	GACNNNNNGTC	1 cut(s)	47
EaeI	YGGCCR	1 cut(s)	532
Eam1104I	CTCTTC	2 cut(s)	886, 1400
Eam1105I	GACNNNNNGTC	1 cut(s)	47
EarI	CTCTTC	2 cut(s)	886, 1400
EciI	GGCGGA	1 cut(s)	1250
Ecl136II	GAGCTC	2 cut(s)	111, 247
Eco130I	CCWWGG	2 cut(s)	480, 1289
Eco24I	GRGCYC	2 cut(s)	113, 249
Eco31I	GGTCTC	2 cut(s)	395, 1339
Eco47I	GGWCC	3 cut(s)	515, 855, 1249
Eco53kI	GAGCTC	2 cut(s)	111, 247
Eco57I	CTGAAG	2 cut(s)	222, 976
Eco81I	CCTNAGG	1 cut(s)	1201
Eco91I	GGTNACC	2 cut(s)	830, 1460
EcoICRI	GAGCTC	2 cut(s)	111, 247
EcoNI	CCTNNNNNAGG	1 cut(s)	1428
EcoO65I	GGTNACC	2 cut(s)	830, 1460
EcoRII	CCWGG	4 cut(s)	496, 529, 1423, 1456
EcoT14I	CCWWGG	2 cut(s)	480, 1289
EcoT38I	GRGCYC	2 cut(s)	113, 249
ErhI	CCWWGG	2 cut(s)	480, 1289
FaeI	CATG	5 cut(s)	9, 330, 1042, 1293, 1414
FalI	AAGNNNNNCTT	5 cut(s)	37, 1344, 1376, 1512, 1544
FaqI	GGGAC	5 cut(s)	54, 317, 670, 1114, 1235
FatI	CATG	5 cut(s)	5, 326, 1038, 1289, 1410
Fnu4HI	GCNGC	5 cut(s)	194, 339, 838, 1441, 1535
FokI	GGATG	3 cut(s)	665, 1109, 1368
FriOI	GRGCYC	2 cut(s)	113, 249
Fsp4HI	GCNGC	5 cut(s)	194, 339, 838, 1441, 1535
FspBI	CTAG	1 cut(s)	1280
GluI	GCNGC	5 cut(s)	194, 339, 838, 1441, 1535
HaeIII	GGCC	7 cut(s)	501, 534, 937, 1179, 1288, 1423, 1456
HapII	CCGG	5 cut(s)	92, 190, 593, 828, 1180
HgaI	GACGC	3 cut(s)	692, 1031, 1247
Hin1I	GRCGYC	1 cut(s)	684
Hin1II	CATG	5 cut(s)	9, 330, 1042, 1293, 1414
HincII	GTYRAC	1 cut(s)	1192
HindII	GTYRAC	1 cut(s)	1192
HinfI	GANTC	8 cut(s)	274, 614, 897, 971, 1143, 1206, 1434, 1446
HpaII	CCGG	5 cut(s)	92, 190, 593, 828, 1180
HphI	GGTGA	2 cut(s)	812, 1492
Hpy166II	GTNNAC	4 cut(s)	539, 605, 1062, 1192
Hpy8I	GTNNAC	4 cut(s)	539, 605, 1062, 1192
Hpy99I	CGWCG	3 cut(s)	557, 638, 1340
HpyAV	CCTTC	6 cut(s)	362, 514, 578, 1278, 1346, 1548
HpyCH4III	ACNGT	9 cut(s)	45, 623, 820, 1055, 1078, 1105, 1121, 1312, 1504
HpyCH4IV	ACGT	9 cut(s)	306, 636, 718, 813, 917, 1274, 1338, 1467, 1553
HpyCH4V	TGCA	4 cut(s)	196, 463, 1062, 1172
HpyF10VI	GCNNNNNNNGC	7 cut(s)	186, 469, 878, 943, 1039, 1294, 1379
HpyF3I	CTNAG	9 cut(s)	162, 270, 440, 542, 901, 962, 1201, 1436, 1509
HpySE526I	ACGT	9 cut(s)	306, 636, 718, 813, 917, 1274, 1338, 1467, 1553
Hsp92I	GRCGYC	1 cut(s)	684
Hsp92II	CATG	5 cut(s)	9, 330, 1042, 1293, 1414
KpnI	GGTACC	1 cut(s)	384
KroI	GCCGGC	1 cut(s)	189
KroNI	GCCGGC	1 cut(s)	191
LmnI	GCTCC	3 cut(s)	116, 244, 703
Lsp1109I	GCAGC	5 cut(s)	180, 325, 849, 1452, 1521
LweI	GCATC	1 cut(s)	955
MaeI	CTAG	1 cut(s)	1280
MaeII	ACGT	9 cut(s)	306, 636, 718, 813, 917, 1274, 1338, 1467, 1553
MaeIII	GTNAC	5 cut(s)	450, 814, 830, 1460, 1549
MboII	GAAGA	7 cut(s)	228, 569, 784, 787, 903, 982, 1387
MfeI	CAATTG	1 cut(s)	123
MhlI	GDGCHC	3 cut(s)	113, 249, 1064
MluCI	AATT	8 cut(s)	64, 123, 153, 446, 572, 1125, 1212, 1217
MlyI	GAGTC	4 cut(s)	965, 1137, 1428, 1455
MmeI	TCCRAC	4 cut(s)	289, 372, 833, 1365
MnlI	CCTC	8 cut(s)	52, 235, 394, 887, 989, 999, 1133, 1210
MroNI	GCCGGC	1 cut(s)	189
MroXI	GAANNNNTTC	2 cut(s)	17, 567
MseI	TTAA	4 cut(s)	332, 735, 1473, 1518
MslI	CAYNNNNRTG	4 cut(s)	125, 362, 791, 812
MspA1I	CMGCKG	1 cut(s)	180
MspI	CCGG	5 cut(s)	92, 190, 593, 828, 1180
MspR9I	CCNGG	4 cut(s)	498, 531, 1425, 1458
MunI	CAATTG	1 cut(s)	123
Mva1269I	GAATGC	1 cut(s)	205
MvaI	CCWGG	4 cut(s)	498, 531, 1425, 1458
MvnI	CGCG	1 cut(s)	881
MwoI	GCNNNNNNNGC	7 cut(s)	186, 469, 878, 943, 1039, 1294, 1379
NaeI	GCCGGC	1 cut(s)	191
NcoI	CCATGG	1 cut(s)	1289
NgoMIV	GCCGGC	1 cut(s)	189
NlaIII	CATG	5 cut(s)	9, 330, 1042, 1293, 1414
NlaIV	GGNNCC	3 cut(s)	382, 525, 1251
NmeAIII	GCCGAG	2 cut(s)	1211, 1469
NmuCI	GTSAC	1 cut(s)	814
OliI	CACNNNNGTG	1 cut(s)	125
PagI	TCATGA	1 cut(s)	1410
PcsI	WCGNNNNNNNCGW	2 cut(s)	1271, 1550
PctI	GAATGC	1 cut(s)	205
PdiI	GCCGGC	1 cut(s)	191
PdmI	GAANNNNTTC	2 cut(s)	17, 567
PfeI	GAWTC	4 cut(s)	274, 614, 897, 1206
PflFI	GACNNNGTC	1 cut(s)	584
PkrI	GCNGC	5 cut(s)	195, 340, 839, 1442, 1536
Ple19I	CGATCG	1 cut(s)	601
PleI	GAGTC	4 cut(s)	965, 1137, 1428, 1454
PpsI	GAGTC	4 cut(s)	965, 1137, 1428, 1454
Psp124BI	GAGCTC	2 cut(s)	113, 249
Psp1406I	AACGTT	2 cut(s)	917, 1553
Psp6I	CCWGG	4 cut(s)	496, 529, 1423, 1456
PspEI	GGTNACC	2 cut(s)	830, 1460
PspGI	CCWGG	4 cut(s)	496, 529, 1423, 1456
PspN4I	GGNNCC	3 cut(s)	382, 525, 1251
PspPI	GGNCC	6 cut(s)	500, 515, 855, 935, 1249, 1421
PstI	CTGCAG	1 cut(s)	198
PstNI	CAGNNNCTG	1 cut(s)	974
PsyI	GACNNNGTC	1 cut(s)	584
PvuI	CGATCG	1 cut(s)	601
RsaI	GTAC	1 cut(s)	382
RsaNI	GTAC	1 cut(s)	381
RseI	CAYNNNNRTG	4 cut(s)	125, 362, 791, 812
SacI	GAGCTC	2 cut(s)	113, 249
SaqAI	TTAA	4 cut(s)	332, 735, 1473, 1518
SatI	GCNGC	5 cut(s)	194, 339, 838, 1441, 1535
Sau96I	GGNCC	6 cut(s)	500, 515, 855, 935, 1249, 1421
SchI	GAGTC	4 cut(s)	965, 1137, 1428, 1455
ScrFI	CCNGG	4 cut(s)	498, 531, 1425, 1458
SduI	GDGCHC	3 cut(s)	113, 249, 1064
SfaNI	GCATC	1 cut(s)	955
SfcI	CTRYAG	1 cut(s)	194
SinI	GGWCC	3 cut(s)	515, 855, 1249
SmiMI	CAYNNNNRTG	4 cut(s)	125, 362, 791, 812
SmlI	CTYRAG	1 cut(s)	1361
SmoI	CTYRAG	1 cut(s)	1361
Sse9I	AATT	8 cut(s)	64, 123, 153, 446, 572, 1125, 1212, 1217
SsiI	CCGC	7 cut(s)	180, 203, 835, 881, 888, 953, 1235
SspMI	CTAG	1 cut(s)	1280
SstI	GAGCTC	2 cut(s)	113, 249
StyD4I	CCNGG	4 cut(s)	496, 529, 1423, 1456
StyI	CCWWGG	2 cut(s)	480, 1289
TaaI	ACNGT	9 cut(s)	45, 623, 820, 1055, 1078, 1105, 1121, 1312, 1504
TaiI	ACGT	9 cut(s)	309, 639, 721, 816, 920, 1277, 1341, 1470, 1556
TaqI	TCGA	5 cut(s)	375, 420, 713, 846, 1304
TaqII	GACCGA	1 cut(s)	532
TasI	AATT	8 cut(s)	64, 123, 153, 446, 572, 1125, 1212, 1217
TfiI	GAWTC	4 cut(s)	274, 614, 897, 1206
Tru1I	TTAA	4 cut(s)	332, 735, 1473, 1518
Tru9I	TTAA	4 cut(s)	332, 735, 1473, 1518
TscAI	CASTG	2 cut(s)	966, 1174
TseFI	GTSAC	1 cut(s)	814
TseI	GCWGC	5 cut(s)	193, 338, 837, 1440, 1534
Tsp45I	GTSAC	1 cut(s)	814
TspDTI	ATGAA	5 cut(s)	832, 1306, 1399, 1427, 1504
TspGWI	ACGGA	2 cut(s)	309, 1416
TspRI	CASTG	2 cut(s)	966, 1174
Tth111I	GACNNNGTC	1 cut(s)	584
VneI	GTGCAC	1 cut(s)	1060
VpaK11BI	GGWCC	3 cut(s)	515, 855, 1249
XagI	CCTNNNNNAGG	1 cut(s)	1428
XapI	RAATTY	2 cut(s)	64, 1125
XmnI	GAANNNNTTC	2 cut(s)	17, 567
XspI	CTAG	1 cut(s)	1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.