Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009744

Physical Location & Seq

Genomic Coordinates Reverse (-)

8493700 .. 8496238

Gene Structure 2539 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016641

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGCTTCCGATCCAATGTGGATGGGAAAGTTTCTACTAGTGTTAGTACTCTTGTCGTCCCCATTTATCCCATTTATTCACTGTGAAAATATCACAAACACGATACAGTCCGAGTGTTTGAAGGTTTCCAACTCGGAGTTTGCTGGGTCGATTGTGAACACCATTGATGTTATGCAGCAGGTGGTGTCTATTCTCTCCAGCGTTGCTAAGGGTTTCGGGGATTTCCGGCTTTCGAATGCCATCGACGACTGTCTGGATTTGATGGATAGCTCGTCTGATGAGTTGAGCTGGACCCTCTCTGCTACCCAGAATCAGAATGGCAAACATAACAGCACTGGGAATTTGAGTTCTGATCTGAGAACATGGCTGAGTGCTACACTGTTTAACCAGGACACATGCAGTGATGGATTTGATGGCACCAACGGCATTGTCAAAGACTTGGTGTCCGGTAGCCTTAACCAAATAACTTCCTTGGTCCTGGAACTACTCGGCCAAGTGCACCCGGTTTCGGATCGGCACGAGTCAAATGGCCAAACCCCAGCATGGTTTAGATCCGAGGACCGCAGACTGCTACAAGCAAATGGAATGCCTGTAGACGTTGTGGTAGCTCAGGATGGAACCGGGAACTTCACAAATATCACCTCCGCGATTTTATCAGTACCGGATTACAGCTTGAAGCGCTACGTGATTTACGTCAAGAGAGGGGTGTACAAGGAGTATGTGGAGATCAAGAAGAAGAAATGGAACATAATGATGATCGGAGATGGCATGGATGCTACTGTAATCAGTGGGAATCACAATTATGTGGATGGTTGGACCACATTTCGCTCTGCAACTTTTGCTGTTAGTGGAAGAGGGTTCATAGCGCGCGACATCACATTCGAGAACACAGCAGGACCCGAGAAGCACATGGCAGTGGCGCTACGATCCGACTCAGACCTCTCGGTCTTTTACCGGTGTGAGTTTCGGGGATACCAGGACACACTATACACACACTCTATGCGCCAATTCTATCGAGACTGCAAAATCAGCGGCACTGTTGATTTCATCTTCGGTGACGGCACTGTTGTGTTCCAGAACTGCCAAATCCTAGCCAGACAAGCCTTGCCAAACCAAAAGAACAGCATCACAGCCCATGGCCGAAAGTACGTAGATGAGCCAACAGGCTTTTCGATCCAATTCTGCAACATATCTGCACATCCAGACTTGTTGGCCACCCCGGTTAACTCTAGCACACCTACATACCTTGGTAGACCATGGAAACAATTTTCAAGAACTATAATCATGCAGTCCTTCATGAGCAATATCATAAAGCCAGAAGGGTGGCTAGAGTGGAATGGTAGCATGTTTTTGGATTCATTGTTCTATGGAGAGTACATGAATTACGGGCCTGGAGCGGGCCTCGGGAGCAGGGTTACCTGGCCGGGATATCAAAAGTTTAACGAGTCCGGGCAGGCCAAGAACTATACAGTGTCCGAGTTTATTGAAGGAAACTTATGGTTGCCCTCTACTGGTGTCAAATATACTGCAGGCTTTGGGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

515

Amino Acids

57.18

Weight (kDa)

5.65

Isoelectric Point (pI)

34.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	62 - 160	6.5e-16	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	199 - 498	3.1e-143	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43270 AT4G33220 AT4G33220
fragaria_vesca	FvH4_1g09610
malus_domestica	MD02G1104600.v1.1 MD02G1105100.v1.1 MD15G1224900.v1.1
prunus_persica	Prupe.7G190300_v2.0.a1
pyrus_communis	pycom02g08260
rosa_chinensis	RchiOBHm_Chr2g0096461
rosa_laevigata	RLG00000016641
rosa_multiflora	Rmu_co8263641.1_g000001 Rmu_sc0004096.1_g000019 Rmu_sc0029740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00145620
rosa_rugosa	Rorug02G0057900
rosa_samantha	Rh2AG104800 Rh2BG107000 Rh2CG108800 Rh2DG108000
rosa_wichuraiana	Rw2G008100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	169
AasI	GACNNNNNNGTC	1 cut(s)	944
Acc36I	ACCTGC	1 cut(s)	169
AccB1I	GGYRCC	1 cut(s)	416
AccBSI	CCGCTC	1 cut(s)	1397
AccI	GTMKAC	2 cut(s)	594, 1252
AccII	CGCG	3 cut(s)	647, 868, 870
AciI	CCGC	4 cut(s)	562, 645, 1032, 1397
AclWI	GGATC	5 cut(s)	5, 519, 546, 921, 1168
AcoI	YGGCCR	5 cut(s)	490, 529, 1138, 1212, 1421
AcsI	RAATTY	1 cut(s)	340
AfaI	GTAC	5 cut(s)	48, 660, 710, 1148, 1376
AfeI	AGCGCT	1 cut(s)	680
AfiI	CCNNNNNNNGG	4 cut(s)	508, 543, 1397, 1511
AgeI	ACCGGT	1 cut(s)	954
AgsI	TTSAA	4 cut(s)	121, 676, 1272, 1487
AhdI	GACNNNNNGTC	1 cut(s)	442
AhlI	ACTAGT	1 cut(s)	37
AjnI	CCWGG	5 cut(s)	387, 477, 975, 1390, 1418
AjuI	GAANNNNNNNTTGG	2 cut(s)	1153, 1185
AleI	CACNNNNGTG	1 cut(s)	1067
AloI	GAACNNNNNNTCC	4 cut(s)	331, 363, 1346, 1378
AluBI	AGCT	4 cut(s)	270, 288, 608, 672
AluI	AGCT	4 cut(s)	270, 288, 608, 672
Alw21I	GWGCWC	1 cut(s)	501
Alw26I	GTCTC	1 cut(s)	1011
Alw44I	GTGCAC	1 cut(s)	497
AlwI	GGATC	5 cut(s)	5, 519, 546, 921, 1168
Ama87I	CYCGRG	2 cut(s)	899, 1403
Aor51HI	AGCGCT	1 cut(s)	680
AoxI	GGCC	8 cut(s)	490, 529, 1138, 1212, 1388, 1399, 1421, 1455
ApaLI	GTGCAC	1 cut(s)	497
ApeKI	GCWGC	1 cut(s)	175
ApoI	RAATTY	1 cut(s)	340
ArsI	GACNNNNNNTTYG	2 cut(s)	863, 895
AsiGI	ACCGGT	1 cut(s)	954
AspLEI	GCGC	5 cut(s)	681, 868, 870, 922, 1005
AspS9I	GGNCC	7 cut(s)	291, 475, 559, 816, 896, 1388, 1399
AsuC2I	CCSGG	5 cut(s)	503, 622, 1220, 1425, 1450
AsuHPI	GGTGA	2 cut(s)	631, 1067
AsuII	TTCGAA	1 cut(s)	233
AvaI	CYCGRG	2 cut(s)	899, 1403
AvaII	GGWCC	5 cut(s)	291, 475, 559, 816, 896
BaeGI	GKGCMC	1 cut(s)	501
BalI	TGGCCA	2 cut(s)	531, 1214
BanI	GGYRCC	1 cut(s)	416
BauI	CACGAG	1 cut(s)	518
Bbv12I	GWGCWC	1 cut(s)	501
BbvI	GCAGC	1 cut(s)	187
BccI	CCATC	8 cut(s)	16, 248, 256, 398, 407, 608, 758, 803
BceAI	ACGGC	2 cut(s)	439, 1075
BciT130I	CCWGG	5 cut(s)	389, 479, 977, 1392, 1420
BciVI	GTATCC	1 cut(s)	965
BcnI	CCSGG	5 cut(s)	503, 622, 1220, 1425, 1450
BcoDI	GTCTC	1 cut(s)	1011
BcuI	ACTAGT	1 cut(s)	37
BfaI	CTAG	4 cut(s)	38, 1091, 1230, 1328
BfmI	CTRYAG	2 cut(s)	591, 1527
BfoI	RGCGCY	2 cut(s)	682, 923
BfuAI	ACCTGC	1 cut(s)	169
BfuI	GTATCC	1 cut(s)	965
BisI	GCNGC	2 cut(s)	176, 1033
BlsI	GCNGC	2 cut(s)	177, 1034
BmcAI	AGTACT	1 cut(s)	48
Bme18I	GGWCC	5 cut(s)	291, 475, 559, 816, 896
BmeRI	GACNNNNNGTC	1 cut(s)	442
BmeT110I	CYCGRG	2 cut(s)	899, 1403
BmgT120I	GGNCC	7 cut(s)	291, 475, 559, 816, 896, 1388, 1399
BmiI	GGNNCC	4 cut(s)	293, 418, 619, 898
BmrI	ACTGGG	1 cut(s)	345
BmsI	GCATC	2 cut(s)	763, 1134
BmuI	ACTGGG	1 cut(s)	345
BoxI	GACNNNNGTC	1 cut(s)	249
BplI	GAGNNNNNCTC	2 cut(s)	1386, 1418
BpmI	CTGGAG	2 cut(s)	181, 1413
Bpu10I	CCTNAGC	2 cut(s)	207, 609
Bpu14I	TTCGAA	1 cut(s)	233
BpuMI	CCSGG	5 cut(s)	503, 622, 1220, 1425, 1450
BsaAI	YACGTR	2 cut(s)	685, 1150
BsaJI	CCNNGG	7 cut(s)	471, 555, 1135, 1218, 1246, 1256, 1402
BsaWI	WCCGGW	3 cut(s)	446, 661, 954
Bsc4I	CCNNNNNNNGG	4 cut(s)	508, 543, 1397, 1511
Bse118I	RCCGGY	1 cut(s)	954
Bse1I	ACTGG	2 cut(s)	340, 1516
BseBI	CCWGG	5 cut(s)	389, 479, 977, 1392, 1420
BseDI	CCNNGG	7 cut(s)	471, 555, 1135, 1218, 1246, 1256, 1402
BseGI	GGATG	5 cut(s)	27, 619, 778, 814, 1198
BseLI	CCNNNNNNNGG	4 cut(s)	508, 543, 1397, 1511
BseMII	CTCAG	4 cut(s)	347, 359, 623, 948
BseNI	ACTGG	2 cut(s)	340, 1516
BsePI	GCGCGC	1 cut(s)	866
BseSI	GKGCMC	1 cut(s)	501
BseXI	GCAGC	1 cut(s)	187
BseYI	CCCAGC	2 cut(s)	143, 538
BsgI	GTGCAG	1 cut(s)	1179
Bsh1236I	CGCG	3 cut(s)	647, 868, 870
BshFI	GGCC	8 cut(s)	492, 531, 1140, 1214, 1390, 1401, 1423, 1457
BshNI	GGYRCC	1 cut(s)	416
BshTI	ACCGGT	1 cut(s)	954
BsiHKAI	GWGCWC	1 cut(s)	501
BsiHKCI	CYCGRG	2 cut(s)	899, 1403
BsiSI	CCGG	9 cut(s)	226, 447, 503, 621, 662, 955, 1220, 1424, 1449
BslFI	GGGAC	1 cut(s)	43
BslI	CCNNNNNNNGG	4 cut(s)	508, 543, 1397, 1511
BsmAI	GTCTC	1 cut(s)	1011
BsmFI	GGGAC	1 cut(s)	43
BsmI	GAATGC	2 cut(s)	241, 591
BsnI	GGCC	8 cut(s)	492, 531, 1140, 1214, 1390, 1401, 1423, 1457
BsoBI	CYCGRG	2 cut(s)	899, 1403
Bsp119I	TTCGAA	1 cut(s)	233
Bsp1286I	GDGCHC	1 cut(s)	501
Bsp1407I	TGTACA	1 cut(s)	708
Bsp143I	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
Bsp19I	CCATGG	2 cut(s)	1135, 1256
BspACI	CCGC	4 cut(s)	562, 645, 1032, 1397
BspANI	GGCC	8 cut(s)	492, 531, 1140, 1214, 1390, 1401, 1423, 1457
BspCNI	CTCAG	4 cut(s)	348, 360, 622, 947
BspFNI	CGCG	3 cut(s)	647, 868, 870
BspHI	TCATGA	1 cut(s)	1296
BspLI	GGNNCC	4 cut(s)	293, 418, 619, 898
BspMAI	CTGCAG	1 cut(s)	1531
BspMI	ACCTGC	1 cut(s)	169
BspPI	GGATC	5 cut(s)	5, 519, 546, 921, 1168
BspT104I	TTCGAA	1 cut(s)	233
BspT107I	GGYRCC	1 cut(s)	416
BsrBI	CCGCTC	1 cut(s)	1397
BsrFI	RCCGGY	1 cut(s)	954
BsrGI	TGTACA	1 cut(s)	708
BsrI	ACTGG	2 cut(s)	340, 1516
BssAI	RCCGGY	1 cut(s)	954
BssECI	CCNNGG	7 cut(s)	471, 555, 1135, 1218, 1246, 1256, 1402
BssHII	GCGCGC	1 cut(s)	866
BssMI	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
BssSI	CACGAG	1 cut(s)	518
BssT1I	CCWWGG	4 cut(s)	471, 1135, 1246, 1256
Bst2BI	CACGAG	1 cut(s)	518
Bst2UI	CCWGG	5 cut(s)	389, 479, 977, 1392, 1420
Bst4CI	ACNGT	8 cut(s)	83, 108, 251, 381, 781, 1039, 1066, 1471
Bst6I	CTCTTC	1 cut(s)	847
BstAPI	GCANNNNNTGC	1 cut(s)	839
BstAUI	TGTACA	1 cut(s)	708
BstBAI	YACGTR	2 cut(s)	685, 1150
BstBI	TTCGAA	1 cut(s)	233
BstC8I	GCNNGC	4 cut(s)	868, 1399, 1455, 1531
BstDEI	CTNAG	5 cut(s)	207, 356, 368, 609, 934
BstDSI	CCRYGG	2 cut(s)	1135, 1256
BstEII	GGTNACC	1 cut(s)	1414
BstF5I	GGATG	5 cut(s)	27, 619, 778, 814, 1198
BstFNI	CGCG	3 cut(s)	647, 868, 870
BstH2I	RGCGCY	2 cut(s)	682, 923
BstHHI	GCGC	5 cut(s)	681, 868, 870, 922, 1005
BstKTI	GATC	8 cut(s)	13, 355, 514, 554, 729, 759, 929, 1176
BstMAI	GTCTC	1 cut(s)	1011
BstMBI	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
BstMWI	GCNNNNNNNGC	6 cut(s)	423, 678, 839, 1029, 1100, 1407
BstNI	CCWGG	5 cut(s)	389, 479, 977, 1392, 1420
BstNSI	RCATGY	2 cut(s)	399, 1348
BstPAI	GACNNNNGTC	1 cut(s)	249
BstPI	GGTNACC	1 cut(s)	1414
BstSFI	CTRYAG	2 cut(s)	591, 1527
BstSLI	GKGCMC	1 cut(s)	501
BstSNI	TACGTA	1 cut(s)	1150
BstUI	CGCG	3 cut(s)	647, 868, 870
BstV1I	GCAGC	1 cut(s)	187
BstX2I	RGATCY	1 cut(s)	551
BstXI	CCANNNNNNTGG	1 cut(s)	1323
BstYI	RGATCY	1 cut(s)	551
BsuI	GTATCC	1 cut(s)	965
BsuRI	GGCC	8 cut(s)	492, 531, 1140, 1214, 1390, 1401, 1423, 1457
BtgI	CCRYGG	2 cut(s)	1135, 1256
BtsCI	GGATG	5 cut(s)	27, 619, 778, 814, 1198
BtsI	GCAGTG	2 cut(s)	406, 921
BtsIMutI	CAGTG	9 cut(s)	79, 333, 377, 406, 793, 921, 1035, 1062, 1476
BveI	ACCTGC	1 cut(s)	169
Cac8I	GCNNGC	4 cut(s)	868, 1399, 1455, 1531
CciI	TCATGA	1 cut(s)	1296
CfoI	GCGC	5 cut(s)	681, 868, 870, 922, 1005
Cfr10I	RCCGGY	1 cut(s)	954
Cfr13I	GGNCC	7 cut(s)	291, 475, 559, 816, 896, 1388, 1399
Csp6I	GTAC	5 cut(s)	47, 659, 709, 1147, 1375
CspAI	ACCGGT	1 cut(s)	954
CviQI	GTAC	5 cut(s)	47, 659, 709, 1147, 1375
DdeI	CTNAG	5 cut(s)	207, 356, 368, 609, 934
DpnI	GATC	8 cut(s)	12, 354, 513, 553, 728, 758, 928, 1175
DpnII	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
DrdI	GACNNNNNNGTC	1 cut(s)	944
DriI	GACNNNNNGTC	1 cut(s)	442
DseDI	GACNNNNNNGTC	1 cut(s)	944
EaeI	YGGCCR	5 cut(s)	490, 529, 1138, 1212, 1421
Eam1104I	CTCTTC	1 cut(s)	847
Eam1105I	GACNNNNNGTC	1 cut(s)	442
EarI	CTCTTC	1 cut(s)	847
Eco105I	TACGTA	1 cut(s)	1150
Eco130I	CCWWGG	4 cut(s)	471, 1135, 1246, 1256
Eco32I	GATATC	1 cut(s)	1430
Eco47I	GGWCC	5 cut(s)	291, 475, 559, 816, 896
Eco47III	AGCGCT	1 cut(s)	680
Eco88I	CYCGRG	2 cut(s)	899, 1403
Eco91I	GGTNACC	1 cut(s)	1414
EcoO109I	RGGNCCY	1 cut(s)	896
EcoO65I	GGTNACC	1 cut(s)	1414
EcoRII	CCWGG	5 cut(s)	387, 477, 975, 1390, 1418
EcoRV	GATATC	1 cut(s)	1430
EcoT14I	CCWWGG	4 cut(s)	471, 1135, 1246, 1256
ErhI	CCWWGG	4 cut(s)	471, 1135, 1246, 1256
FaqI	GGGAC	1 cut(s)	43
FauI	CCCGC	1 cut(s)	1390
FblI	GTMKAC	2 cut(s)	594, 1252
Fnu4HI	GCNGC	2 cut(s)	176, 1033
FokI	GGATG	5 cut(s)	34, 626, 785, 821, 1185
Fsp4HI	GCNGC	2 cut(s)	176, 1033
FspBI	CTAG	4 cut(s)	38, 1091, 1230, 1328
GlaI	GCGC	5 cut(s)	680, 867, 869, 921, 1004
GluI	GCNGC	2 cut(s)	176, 1033
GsaI	CCCAGC	2 cut(s)	147, 542
GsuI	CTGGAG	2 cut(s)	181, 1413
HaeII	RGCGCY	2 cut(s)	682, 923
HaeIII	GGCC	8 cut(s)	492, 531, 1140, 1214, 1390, 1401, 1423, 1457
HapII	CCGG	9 cut(s)	226, 447, 503, 621, 662, 955, 1220, 1424, 1449
HhaI	GCGC	5 cut(s)	681, 868, 870, 922, 1005
Hin6I	GCGC	5 cut(s)	679, 866, 868, 920, 1003
HinP1I	GCGC	5 cut(s)	679, 866, 868, 920, 1003
HincII	GTYRAC	1 cut(s)	1225
HindII	GTYRAC	1 cut(s)	1225
HinfI	GANTC	6 cut(s)	310, 521, 793, 932, 1355, 1445
HpaI	GTTAAC	1 cut(s)	1225
HpaII	CCGG	9 cut(s)	226, 447, 503, 621, 662, 955, 1220, 1424, 1449
HphI	GGTGA	2 cut(s)	631, 1067
Hpy166II	GTNNAC	6 cut(s)	157, 499, 595, 709, 1225, 1253
Hpy8I	GTNNAC	6 cut(s)	157, 499, 595, 709, 1225, 1253
Hpy99I	CGWCG	1 cut(s)	248
HpyAV	CCTTC	4 cut(s)	115, 1303, 1313, 1481
HpyCH4III	ACNGT	8 cut(s)	83, 108, 251, 381, 781, 1039, 1066, 1471
HpyCH4IV	ACGT	4 cut(s)	597, 684, 693, 1149
HpyCH4V	TGCA	9 cut(s)	175, 399, 499, 833, 1023, 1185, 1196, 1288, 1529
HpyF10VI	GCNNNNNNNGC	6 cut(s)	423, 678, 839, 1029, 1100, 1407
HpyF3I	CTNAG	5 cut(s)	207, 356, 368, 609, 934
HpySE526I	ACGT	4 cut(s)	597, 684, 693, 1149
HspAI	GCGC	5 cut(s)	679, 866, 868, 920, 1003
KspAI	GTTAAC	1 cut(s)	1225
Kzo9I	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
LmnI	GCTCC	2 cut(s)	1394, 1407
Lsp1109I	GCAGC	1 cut(s)	187
LweI	GCATC	2 cut(s)	763, 1134
MaeI	CTAG	4 cut(s)	38, 1091, 1230, 1328
MaeII	ACGT	4 cut(s)	597, 684, 693, 1149
MaeIII	GTNAC	2 cut(s)	1055, 1414
MalI	GATC	8 cut(s)	12, 354, 513, 553, 728, 758, 928, 1175
MbiI	CCGCTC	1 cut(s)	1397
MboI	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
MboII	GAAGA	4 cut(s)	745, 748, 864, 1042
MflI	RGATCY	1 cut(s)	551
MhlI	GDGCHC	1 cut(s)	501
MlsI	TGGCCA	2 cut(s)	531, 1214
MluCI	AATT	6 cut(s)	340, 799, 1007, 1178, 1265, 1381
MluNI	TGGCCA	2 cut(s)	531, 1214
MlyI	GAGTC	3 cut(s)	530, 926, 1454
MmeI	TCCRAC	3 cut(s)	153, 794, 954
MnlI	CCTC	8 cut(s)	305, 550, 652, 695, 848, 950, 1412, 1516
Mox20I	TGGCCA	2 cut(s)	531, 1214
MscI	TGGCCA	2 cut(s)	531, 1214
MseI	TTAA	5 cut(s)	384, 456, 1224, 1440, 1543
MslI	CAYNNNNRTG	4 cut(s)	752, 801, 914, 1067
Msp20I	TGGCCA	2 cut(s)	531, 1214
MspA1I	CMGCKG	1 cut(s)	1032
MspI	CCGG	9 cut(s)	226, 447, 503, 621, 662, 955, 1220, 1424, 1449
Mva1269I	GAATGC	2 cut(s)	241, 591
MvaI	CCWGG	5 cut(s)	389, 479, 977, 1392, 1420
MvnI	CGCG	3 cut(s)	647, 868, 870
MwoI	GCNNNNNNNGC	6 cut(s)	423, 678, 839, 1029, 1100, 1407
NciI	CCSGG	5 cut(s)	503, 622, 1220, 1425, 1450
NcoI	CCATGG	2 cut(s)	1135, 1256
NdeII	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
NlaIV	GGNNCC	4 cut(s)	293, 418, 619, 898
NmeAIII	GCCGAG	1 cut(s)	468
NmuCI	GTSAC	1 cut(s)	1055
NspI	RCATGY	2 cut(s)	399, 1348
NspV	TTCGAA	1 cut(s)	233
OliI	CACNNNNGTG	1 cut(s)	1067
PagI	TCATGA	1 cut(s)	1296
PaqCI	CACCTGC	1 cut(s)	169
PauI	GCGCGC	1 cut(s)	866
PcsI	WCGNNNNNNNCGW	1 cut(s)	690
PctI	GAATGC	2 cut(s)	241, 591
PfeI	GAWTC	3 cut(s)	310, 793, 1355
PfoI	TCCNGGA	1 cut(s)	477
PinAI	ACCGGT	1 cut(s)	954
PkrI	GCNGC	2 cut(s)	177, 1034
PleI	GAGTC	3 cut(s)	529, 926, 1453
PpsI	GAGTC	3 cut(s)	529, 926, 1453
Ppu21I	YACGTR	2 cut(s)	685, 1150
PpuMI	RGGWCCY	1 cut(s)	896
PshAI	GACNNNNGTC	1 cut(s)	249
Psp5II	RGGWCCY	1 cut(s)	896
Psp6I	CCWGG	5 cut(s)	387, 477, 975, 1390, 1418
PspEI	GGTNACC	1 cut(s)	1414
PspFI	CCCAGC	2 cut(s)	143, 538
PspGI	CCWGG	5 cut(s)	387, 477, 975, 1390, 1418
PspN4I	GGNNCC	4 cut(s)	293, 418, 619, 898
PspPI	GGNCC	7 cut(s)	291, 475, 559, 816, 896, 1388, 1399
PspPPI	RGGWCCY	1 cut(s)	896
PstI	CTGCAG	1 cut(s)	1531
PsuI	RGATCY	1 cut(s)	551
PteI	GCGCGC	1 cut(s)	866
RsaI	GTAC	5 cut(s)	48, 660, 710, 1148, 1376
RsaNI	GTAC	5 cut(s)	47, 659, 709, 1147, 1375
RseI	CAYNNNNRTG	4 cut(s)	752, 801, 914, 1067
SaqAI	TTAA	5 cut(s)	384, 456, 1224, 1440, 1543
SatI	GCNGC	2 cut(s)	176, 1033
Sau3AI	GATC	8 cut(s)	10, 352, 511, 551, 726, 756, 926, 1173
Sau96I	GGNCC	7 cut(s)	291, 475, 559, 816, 896, 1388, 1399
ScaI	AGTACT	1 cut(s)	48
SchI	GAGTC	3 cut(s)	530, 926, 1454
SduI	GDGCHC	1 cut(s)	501
SfaNI	GCATC	2 cut(s)	763, 1134
SfcI	CTRYAG	2 cut(s)	591, 1527
SfuI	TTCGAA	1 cut(s)	233
SinI	GGWCC	5 cut(s)	291, 475, 559, 816, 896
SmiMI	CAYNNNNRTG	4 cut(s)	752, 801, 914, 1067
SnaBI	TACGTA	1 cut(s)	1150
SpeI	ACTAGT	1 cut(s)	37
Sse9I	AATT	6 cut(s)	340, 799, 1007, 1178, 1265, 1381
SsiI	CCGC	4 cut(s)	562, 645, 1032, 1397
SspMI	CTAG	4 cut(s)	38, 1091, 1230, 1328
StyI	CCWWGG	4 cut(s)	471, 1135, 1246, 1256
TaaI	ACNGT	8 cut(s)	83, 108, 251, 381, 781, 1039, 1066, 1471
TaiI	ACGT	4 cut(s)	600, 687, 696, 1152
TaqI	TCGA	6 cut(s)	149, 233, 243, 882, 1015, 1172
TaqII	GACCGA	1 cut(s)	934
TasI	AATT	6 cut(s)	340, 799, 1007, 1178, 1265, 1381
TatI	WGTACW	3 cut(s)	46, 708, 1374
TauI	GCSGC	1 cut(s)	1035
TfiI	GAWTC	3 cut(s)	310, 793, 1355
Tru1I	TTAA	5 cut(s)	384, 456, 1224, 1440, 1543
Tru9I	TTAA	5 cut(s)	384, 456, 1224, 1440, 1543
TscAI	CASTG	9 cut(s)	86, 340, 384, 406, 793, 921, 1042, 1069, 1476
TseFI	GTSAC	1 cut(s)	1055
TseI	GCWGC	1 cut(s)	175
Tsp45I	GTSAC	1 cut(s)	1055
TspDTI	ATGAA	5 cut(s)	850, 1036, 1285, 1347, 1394
TspRI	CASTG	9 cut(s)	86, 340, 384, 406, 793, 921, 1042, 1069, 1476
VneI	GTGCAC	1 cut(s)	497
VpaK11BI	GGWCC	5 cut(s)	291, 475, 559, 816, 896
XapI	RAATTY	1 cut(s)	340
XceI	RCATGY	2 cut(s)	399, 1348
XcmI	CCANNNNNNNNNTGG	1 cut(s)	314
XmiI	GTMKAC	2 cut(s)	594, 1252
XspI	CTAG	4 cut(s)	38, 1091, 1230, 1328
ZrmI	AGTACT	1 cut(s)	48

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.