pycom02g08260

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009744
Physical Location & Seq
Reverse (-)
5759213 .. 5760060
848 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08260.1

Sequence Viewer

Length: 720 bp
ATGACTAAAGTGAAACATGAGTATAAGTTTAGGACCATTGAAATCGTATTCCATCATTCATATTTTATATTACGAAAACTGATCCTGTTTTCATTGAACTATGTATTCAGCTGTGAGTGTCAGGGTTTCATAGCCAGAGACATAACATTCGAAAACACGGCAGGGCCACAGAAGCACCAGGTAGTAGCCCCAAGATCGGATTCCGAACTATCAGTGTTCAACAGATGCCAAATAAGAGGGTACCAAGACACACTCTACGCACACACGATGCGCCAGTTCTACAGAGATTGCAAAATAAGTAGTACAGTTGACTTCATTTTTGGTGATGCAAGTGTTTTGTTCCAAAACTGCCAGATCGATCATCCAGATACCCAAGGCCGGAAGGACCCGAATGAGCCGACCGGAATCTCAATCCATTTCTGCAACATTACTGCAGATGCAGACATGTTACCCTTTGTTAACTCAACTTTTACTTACCTTGGTAGGACTTGGAAGCTATATAGCCTGTATTCAAGAATTGTAATAATGCAGTCTTTCTTGAGAAATGCCATGATGCCTGAGGGGTGGAGTACGTGCCCGGGCTGCCGAGTATTTAATGAGTCCACTCAGGCCAAAACTATACTGTGGCTCAATTTATTGAAGGCAACCTGTGGTTGCGCACTACTGATGTCAAATAACTGCAGGATTTGGGGCTTGAGGATTCAAGCAGTTGATGTGTAA

Protein Analysis

240

Amino Acids

27.62

Weight (kDa)

8.63

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 36 - 191 5.2e-61 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 658
Acc65I GGTACC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 240
AclWI GGATC 1 cut(s) 76
AfaI GTAC 3 cut(s) 242, 304, 571
AfiI CCNNNNNNNGG 2 cut(s) 196, 378
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 6 cut(s) 41, 97, 220, 513, 640, 704
AjnI CCWGG 1 cut(s) 177
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 2 cut(s) 111, 496
AluI AGCT 2 cut(s) 111, 496
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 577
AoxI GGCC 3 cut(s) 164, 376, 609
ApeKI GCWGC 1 cut(s) 582
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 302, 334
Asp718I GGTACC 1 cut(s) 240
AspLEI GCGC 2 cut(s) 273, 659
AspS9I GGNCC 3 cut(s) 33, 164, 385
AsuC2I CCSGG 2 cut(s) 578, 579
AsuHPI GGTGA 1 cut(s) 335
AsuII TTCGAA 1 cut(s) 150
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 2 cut(s) 33, 385
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 569
BccI CCATC 1 cut(s) 60
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 1 cut(s) 179
BcnI CCSGG 2 cut(s) 578, 579
BcoDI GTCTC 1 cut(s) 132
BfmI CTRYAG 3 cut(s) 280, 432, 679
BisI GCNGC 1 cut(s) 583
BlsI GCNGC 1 cut(s) 584
Bme1390I CCNGG 3 cut(s) 179, 578, 579
Bme18I GGWCC 2 cut(s) 33, 385
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 3 cut(s) 33, 164, 385
BmiI GGNNCC 2 cut(s) 242, 387
BmrFI CCNGG 3 cut(s) 179, 578, 579
BmsI GCATC 5 cut(s) 215, 258, 316, 427, 543
Bpu14I TTCGAA 1 cut(s) 150
BpuEI CTTGAG 2 cut(s) 559, 715
BpuMI CCSGG 2 cut(s) 578, 579
Bsa29I ATCGAT 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 373, 478, 577
BsaWI WCCGGW 1 cut(s) 401
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 378
Bse1I ACTGG 1 cut(s) 274
Bse21I CCTNAGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 373, 478, 577
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 2 cut(s) 196, 378
BseMII CTCAG 2 cut(s) 549, 620
BseNI ACTGG 1 cut(s) 274
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 3 cut(s) 166, 378, 611
BshNI GGYRCC 1 cut(s) 240
BshVI ATCGAT 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 3 cut(s) 379, 402, 578
BslI CCNNNNNNNGG 2 cut(s) 196, 378
BsmAI GTCTC 1 cut(s) 132
BsnI GGCC 3 cut(s) 166, 378, 611
BsoBI CYCGRG 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 578
Bsp143I GATC 4 cut(s) 81, 194, 354, 358
BspANI GGCC 3 cut(s) 166, 378, 611
BspCNI CTCAG 2 cut(s) 550, 619
BspDI ATCGAT 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 242, 387
BspMAI CTGCAG 2 cut(s) 436, 683
BspPI GGATC 1 cut(s) 76
BspT104I TTCGAA 1 cut(s) 150
BspT107I GGYRCC 1 cut(s) 240
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 3 cut(s) 373, 478, 577
BssMI GATC 4 cut(s) 81, 194, 354, 358
BssT1I CCWWGG 2 cut(s) 373, 478
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 307, 624
BstBAI YACGTR 1 cut(s) 573
BstBI TTCGAA 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 558, 606
BstF5I GGATG 1 cut(s) 361
BstHHI GCGC 2 cut(s) 273, 659
BstKTI GATC 4 cut(s) 84, 197, 357, 361
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 4 cut(s) 81, 194, 354, 358
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 2 cut(s) 172, 582
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 3 cut(s) 177, 576, 577
BstSFI CTRYAG 3 cut(s) 280, 432, 679
BstSLI GKGCMC 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 569
Bsu15I ATCGAT 1 cut(s) 357
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 3 cut(s) 166, 378, 611
BsuTUI ATCGAT 1 cut(s) 357
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 219
CfoI GCGC 2 cut(s) 273, 659
Cfr13I GGNCC 3 cut(s) 33, 164, 385
Cfr9I CCCGGG 1 cut(s) 577
ClaI ATCGAT 1 cut(s) 357
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 3 cut(s) 241, 303, 570
CviAII CATG 3 cut(s) 17, 445, 550
CviQI GTAC 3 cut(s) 241, 303, 570
DdeI CTNAG 2 cut(s) 558, 606
DpnI GATC 4 cut(s) 83, 196, 356, 360
DpnII GATC 4 cut(s) 81, 194, 354, 358
Eco130I CCWWGG 2 cut(s) 373, 478
Eco47I GGWCC 2 cut(s) 33, 385
Eco81I CCTNAGG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 373, 478
ErhI CCWWGG 2 cut(s) 373, 478
FaeI CATG 3 cut(s) 20, 448, 553
FatI CATG 3 cut(s) 16, 444, 549
Fnu4HI GCNGC 1 cut(s) 583
FokI GGATG 1 cut(s) 348
Fsp4HI GCNGC 1 cut(s) 583
FspI TGCGCA 1 cut(s) 658
GlaI GCGC 2 cut(s) 272, 658
GluI GCNGC 1 cut(s) 583
HaeIII GGCC 3 cut(s) 166, 378, 611
HapII CCGG 3 cut(s) 379, 402, 578
HhaI GCGC 2 cut(s) 273, 659
Hin1II CATG 3 cut(s) 20, 448, 553
Hin6I GCGC 2 cut(s) 271, 657
HinP1I GCGC 2 cut(s) 271, 657
HincII GTYRAC 2 cut(s) 310, 460
HindII GTYRAC 2 cut(s) 310, 460
HinfI GANTC 4 cut(s) 200, 405, 599, 700
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 379, 402, 578
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 3 cut(s) 310, 460, 603
Hpy188I TCNGA 2 cut(s) 199, 205
Hpy188III TCNNGA 3 cut(s) 365, 513, 538
Hpy8I GTNNAC 3 cut(s) 310, 460, 603
HpyAV CCTTC 2 cut(s) 376, 634
HpyCH4III ACNGT 2 cut(s) 307, 624
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 7 cut(s) 291, 329, 423, 434, 440, 529, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 582
HpyF3I CTNAG 2 cut(s) 558, 606
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 3 cut(s) 20, 448, 553
HspAI GCGC 2 cut(s) 271, 657
KpnI GGTACC 1 cut(s) 244
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 4 cut(s) 81, 194, 354, 358
Lsp1109I GCAGC 1 cut(s) 569
LweI GCATC 5 cut(s) 215, 258, 316, 427, 543
MabI ACCWGGT 1 cut(s) 177
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 1 cut(s) 447
MalI GATC 4 cut(s) 83, 196, 356, 360
MboI GATC 4 cut(s) 81, 194, 354, 358
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 2 cut(s) 516, 631
MlyI GAGTC 1 cut(s) 608
MnlI CCTC 3 cut(s) 230, 553, 690
MseI TTAA 2 cut(s) 459, 594
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 3 cut(s) 379, 402, 578
MspR9I CCNGG 3 cut(s) 179, 578, 579
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 172, 582
NciI CCSGG 2 cut(s) 578, 579
NdeII GATC 4 cut(s) 81, 194, 354, 358
NlaIII CATG 3 cut(s) 20, 448, 553
NlaIV GGNNCC 2 cut(s) 242, 387
NmeAIII GCCGAG 1 cut(s) 611
NsbI TGCGCA 1 cut(s) 658
NspI RCATGY 1 cut(s) 448
NspV TTCGAA 1 cut(s) 150
PciI ACATGT 1 cut(s) 444
PfeI GAWTC 3 cut(s) 200, 405, 700
PkrI GCNGC 1 cut(s) 584
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 573
PpuMI RGGWCCY 1 cut(s) 385
PscI ACATGT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 242, 387
PspPI GGNCC 3 cut(s) 33, 164, 385
PspPPI RGGWCCY 1 cut(s) 385
PstI CTGCAG 2 cut(s) 436, 683
PvuII CAGCTG 1 cut(s) 111
RsaI GTAC 3 cut(s) 242, 304, 571
RsaNI GTAC 3 cut(s) 241, 303, 570
SaqAI TTAA 2 cut(s) 459, 594
SatI GCNGC 1 cut(s) 583
Sau3AI GATC 4 cut(s) 81, 194, 354, 358
Sau96I GGNCC 3 cut(s) 33, 164, 385
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 3 cut(s) 179, 578, 579
SduI GDGCHC 1 cut(s) 578
SetI ASST 6 cut(s) 113, 183, 480, 498, 575, 650
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 5 cut(s) 215, 258, 316, 427, 543
SfcI CTRYAG 3 cut(s) 280, 432, 679
SfuI TTCGAA 1 cut(s) 150
SinI GGWCC 2 cut(s) 33, 385
SmaI CCCGGG 1 cut(s) 579
SmlI CTYRAG 2 cut(s) 538, 694
SmoI CTYRAG 2 cut(s) 538, 694
SrfI GCCCGGGC 1 cut(s) 579
Sse9I AATT 2 cut(s) 516, 631
StyD4I CCNGG 3 cut(s) 177, 576, 577
StyI CCWWGG 2 cut(s) 373, 478
TaaI ACNGT 2 cut(s) 307, 624
TaiI ACGT 1 cut(s) 575
TaqI TCGA 2 cut(s) 150, 357
TasI AATT 2 cut(s) 516, 631
TatI WGTACW 1 cut(s) 302
TfiI GAWTC 3 cut(s) 200, 405, 700
Tru1I TTAA 2 cut(s) 459, 594
Tru9I TTAA 2 cut(s) 459, 594
TscAI CASTG 1 cut(s) 219
TseI GCWGC 1 cut(s) 582
TspDTI ATGAA 4 cut(s) 48, 81, 118, 304
TspMI CCCGGG 1 cut(s) 577
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 33, 385
XceI RCATGY 1 cut(s) 448
XmaI CCCGGG 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.