MD02G1105100.v1.1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009744
Physical Location & Seq
Reverse (-)
8500971 .. 8501525
555 bp
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UTR
Exon/CDS
Intron
MD02G1105100.v1.1.491

Sequence Viewer

Length: 555 bp
ATGCAAAATATTATGAAAGAAGAAATATATAAATATTGCAAAATAAGTGGTACAGTTGACTTCATTTTTGGTGATGCAACTGTTGTGTTCCAAAACTGCCGGATCTTAGCCAAAAAAGGCCTACCGAATCAGAAGAACAGCGTCACATCCCAAGGCCGGAAAGACCCGAATGAGCCGACCGGATTCTCAATCCAGTTCTGCAACATAACTGCAGATGCAGACTTGTTACCCTTGGTCAACTCCACTTTTACTTACCTTGGTAGGCCTTGGAAGCTTTATTCAAGAACTGTAATAATGCATTCTTTCTTGAGCAATGCCATAAGGCCTGAGGGGTGGCTTGAATGGAACGCGGATTTTGCTTTGAGCACGTTGTCTTATGGTGAGTACATGAATTATGGGCCTGGAGCGGGCCTCGGAAGCCGGGTCAAGTGGCCTGGCTACCGAGTCTTTAATGAGTCTACTCAGGTAAAGAACTATTCTGTGGCCCAATTTATTGAAGGCAACCTGTGGTTACCCCCTACTGGTGTCAAATATACAGCAGGATTTGGGGCTTGA

Protein Analysis

185

Amino Acids

20.65

Weight (kDa)

8.93

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 8 - 168 1.2e-71 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 407
AccI GTMKAC 1 cut(s) 458
AccII CGCG 1 cut(s) 350
AciI CCGC 2 cut(s) 350, 407
AclWI GGATC 1 cut(s) 110
AfaI GTAC 2 cut(s) 52, 386
AfiI CCNNNNNNNGG 3 cut(s) 156, 407, 521
AgsI TTSAA 3 cut(s) 282, 341, 497
AjnI CCWGG 2 cut(s) 400, 433
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw21I GWGCWC 1 cut(s) 368
AlwI GGATC 1 cut(s) 110
AoxI GGCC 8 cut(s) 118, 154, 263, 323, 398, 409, 431, 483
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
AspS9I GGNCC 3 cut(s) 398, 409, 484
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 83, 392
AxyI CCTNAGG 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 368
BciT130I CCWGG 2 cut(s) 402, 435
BcnI CCSGG 1 cut(s) 422
BfmI CTRYAG 1 cut(s) 210
Bme1390I CCNGG 3 cut(s) 402, 422, 435
BmgT120I GGNCC 3 cut(s) 398, 409, 484
BmrFI CCNGG 3 cut(s) 402, 422, 435
BmsI GCATC 2 cut(s) 64, 205
BplI GAGNNNNNCTC 2 cut(s) 396, 428
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 422
BsaJI CCNNGG 5 cut(s) 151, 231, 256, 266, 412
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 407, 521
Bse1I ACTGG 2 cut(s) 193, 526
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 2 cut(s) 402, 435
BseDI CCNNGG 5 cut(s) 151, 231, 256, 266, 412
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 3 cut(s) 156, 407, 521
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 318, 476
BseNI ACTGG 2 cut(s) 193, 526
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 8 cut(s) 120, 156, 265, 325, 400, 411, 433, 485
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 4 cut(s) 100, 157, 180, 421
BslI CCNNNNNNNGG 3 cut(s) 156, 407, 521
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 8 cut(s) 120, 156, 265, 325, 400, 411, 433, 485
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 2 cut(s) 350, 407
BspANI GGCC 8 cut(s) 120, 156, 265, 325, 400, 411, 433, 485
BspCNI CTCAG 2 cut(s) 319, 475
BspFNI CGCG 1 cut(s) 350
BspMAI CTGCAG 1 cut(s) 214
BspPI GGATC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 2 cut(s) 193, 526
BssECI CCNNGG 5 cut(s) 151, 231, 256, 266, 412
BssMI GATC 1 cut(s) 102
BssT1I CCWWGG 4 cut(s) 151, 231, 256, 266
Bst2UI CCWGG 2 cut(s) 402, 435
Bst4CI ACNGT 3 cut(s) 55, 82, 289
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 3 cut(s) 106, 327, 462
BstEII GGTNACC 1 cut(s) 510
BstF5I GGATG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 350
BstKTI GATC 1 cut(s) 105
BstMBI GATC 1 cut(s) 102
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 271, 356, 417
BstNI CCWGG 2 cut(s) 402, 435
BstPI GGTNACC 1 cut(s) 510
BstSCI CCNGG 3 cut(s) 400, 420, 433
BstSFI CTRYAG 1 cut(s) 210
BstUI CGCG 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu36I CCTNAGG 1 cut(s) 327
BsuRI GGCC 8 cut(s) 120, 156, 265, 325, 400, 411, 433, 485
BtsCI GGATG 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 409
Cfr13I GGNCC 3 cut(s) 398, 409, 484
CseI GACGC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 51, 385
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 1 cut(s) 388
CviQI GTAC 2 cut(s) 51, 385
DdeI CTNAG 3 cut(s) 106, 327, 462
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eco130I CCWWGG 4 cut(s) 151, 231, 256, 266
Eco147I AGGCCT 3 cut(s) 120, 265, 325
Eco81I CCTNAGG 1 cut(s) 327
Eco91I GGTNACC 1 cut(s) 510
EcoO65I GGTNACC 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 400, 433
EcoT14I CCWWGG 4 cut(s) 151, 231, 256, 266
EcoT22I ATGCAT 1 cut(s) 300
ErhI CCWWGG 4 cut(s) 151, 231, 256, 266
FaeI CATG 1 cut(s) 391
FaiI YATR 9 cut(s) 14, 28, 30, 206, 320, 378, 389, 396, 534
FatI CATG 1 cut(s) 387
FauI CCCGC 1 cut(s) 400
FblI GTMKAC 1 cut(s) 458
FokI GGATG 1 cut(s) 133
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 8 cut(s) 120, 156, 265, 325, 400, 411, 433, 485
HapII CCGG 4 cut(s) 100, 157, 180, 421
HgaI GACGC 1 cut(s) 130
Hin1II CATG 1 cut(s) 391
HincII GTYRAC 2 cut(s) 58, 238
HindII GTYRAC 2 cut(s) 58, 238
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 4 cut(s) 127, 183, 444, 455
HpaII CCGG 4 cut(s) 100, 157, 180, 421
HphI GGTGA 2 cut(s) 83, 392
Hpy166II GTNNAC 3 cut(s) 58, 238, 459
Hpy188I TCNGA 2 cut(s) 132, 416
Hpy188III TCNNGA 2 cut(s) 282, 307
Hpy8I GTNNAC 3 cut(s) 58, 238, 459
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 3 cut(s) 55, 82, 289
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 7 cut(s) 4, 39, 77, 201, 212, 218, 298
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 356, 417
HpyF3I CTNAG 3 cut(s) 106, 327, 462
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 1 cut(s) 391
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 404
LweI GCATC 2 cut(s) 64, 205
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 3 cut(s) 142, 225, 510
MalI GATC 1 cut(s) 104
MbiI CCGCTC 1 cut(s) 407
MboI GATC 1 cut(s) 102
MboII GAAGA 2 cut(s) 32, 145
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 2 cut(s) 391, 488
MlyI GAGTC 2 cut(s) 453, 464
MnlI CCTC 2 cut(s) 322, 422
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 1 cut(s) 450
MspI CCGG 4 cut(s) 100, 157, 180, 421
MspR9I CCNGG 3 cut(s) 402, 422, 435
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 2 cut(s) 402, 435
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 3 cut(s) 271, 356, 417
NciI CCSGG 1 cut(s) 422
NdeII GATC 1 cut(s) 102
NlaIII CATG 1 cut(s) 391
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 300
PceI AGGCCT 3 cut(s) 120, 265, 325
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 127, 183
PleI GAGTC 2 cut(s) 452, 463
PpsI GAGTC 2 cut(s) 452, 463
Psp6I CCWGG 2 cut(s) 400, 433
PspEI GGTNACC 1 cut(s) 510
PspGI CCWGG 2 cut(s) 400, 433
PspPI GGNCC 3 cut(s) 398, 409, 484
PstI CTGCAG 1 cut(s) 214
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 2 cut(s) 52, 386
RsaNI GTAC 2 cut(s) 51, 385
SaqAI TTAA 1 cut(s) 450
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 3 cut(s) 398, 409, 484
SchI GAGTC 2 cut(s) 453, 464
ScrFI CCNGG 3 cut(s) 402, 422, 435
SduI GDGCHC 1 cut(s) 368
SetI ASST 5 cut(s) 258, 276, 371, 468, 507
SfaNI GCATC 2 cut(s) 64, 205
SfcI CTRYAG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 2 cut(s) 391, 488
SseBI AGGCCT 3 cut(s) 120, 265, 325
SsiI CCGC 2 cut(s) 350, 407
SspI AATATT 2 cut(s) 10, 35
StuI AGGCCT 3 cut(s) 120, 265, 325
StyD4I CCNGG 3 cut(s) 400, 420, 433
StyI CCWWGG 4 cut(s) 151, 231, 256, 266
TaaI ACNGT 3 cut(s) 55, 82, 289
TaiI ACGT 1 cut(s) 371
TasI AATT 2 cut(s) 391, 488
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 2 cut(s) 127, 183
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 3 cut(s) 29, 52, 404
XmiI GTMKAC 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.