Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009744

Physical Location & Seq

Genomic Coordinates Reverse (-)

18284995 .. 18288746

Gene Structure 3752 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1224900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGCTTCTGGGAAGCTGCTTTTTTTGCTAGTCTTATTGTGTGCCTGCTCCAAAAGCAGAATCCCTTTTGCTAGCTGCAGCATCACAAATGATATACCGTCAGAGTGCTTGAAAGTGCCTGCATCCGAATTTGCAGGCTCCTTAAGGGACACCATTGGTGCTGTGCAGCAAGTGGCCTCCATACTCTCTCAGTTTGCAAATGCTTTCGGCGATTTTCGCCTTTCCAACGCCATTTCCGACTGCCTCGATTTGCTTGACTTCTCTGCTGATGAGCTGAACTGGTCTCTCTCTGCTTCCCAGAACCCGGAAGGCAAGGACAACAGCACAGGGAAACTAAGTTCTGATCTGAGGACATGGTTGAGCGCTGCACTGGTTAACCAGGACACATGCAGTGATGGGTTCGAAGGCACCAACAGCATTGTCAAAGGGTTGGTCTCAACTGGCCTCAACCAAGTTACCTCTTTAGTCCAAGGCCTCCTCACCCAAGTGCACCCTAATACCGATCACCACGGGCCTAATGGAGAAATCCCTTCGTGGGTTAAAACCGAAGATCGAAAACTTCTACAAGCCGGTGGTGTCAATGTGGATGCAGTTGTAGCCCAAGATGGTACCGGGAATTTCACAAATGTCATGGATGCCGTGTTAGCAGCGCCGGATGAAAGGATGACCCGCTATGTTATATACATCAAGAAAGGCACATACAAAGAGAACGTGGAGATCAAGAAGAAGAAATGGAACCTAATGATGATTGGAGATGGTATGGATGCTACTATAATCTCTGGAAATCGAAATTTTGTCGATGGTTGGACCACATTTCGATCCGCAACATTTGCTGTGAGTGGTAGAGGGTTCATAGCAAGAGACCTAACGTTCGAGAACACGGCAGGGCCAGAGAAGCACCAGGCAGTTGCCCTAAGATCGGATTCCGACCTATCGGTGTTCTACAGATGCCAAATAAGAGGCTACCAAGACACACTCTACGCACACGCAATGCGCCAGTTTTACAGAGATTGCAAAATAAGTGGCACAGTTGATTTCATTTTTGGTGATGCAACTGTTGTGTTCCAAAACTGCCAGATCCTAGCCAAAAAAGGCCTACCAAATCAGAAGAACAGCGTCACGGCCCAAGGCCGGAAGGACCCGAATGAGCCGACCGGAATCTCAATCCAGTTCTGCAACATAACTGCAGATGCAGACTTGTTACCCCTTGTCAACTCAACTTCTACTTACCTTGGTAGGCCTTGGAAGCTGTATTCAAGAACTGTAATAATGCAGTCCTTCTTGAGCAATGCCATAAGGCCTGAGGGGTGGCTCGAGTGGAATGCGGATTTTGCTTTGAGCACATTGTCTTATGGGGAGTACATGAATTATGGGCCTGGAGCGGGGCTCGAAAGCCGGGTCAAATGGCCCGGCTACCGAGTGTTTAATGAGTCTACTCAAGTCCAGAACTATACTGTAGCCCAATTTATTGAAGGAGACCTGTGGTTGCCCCCTACTGGTGTCAAATATATAGCAGGACTTGGGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

510

Amino Acids

55.74

Weight (kDa)

5.69

Isoelectric Point (pI)

21.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	37 - 155	8.6e-18	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	196 - 493	1.6e-149	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43270 AT4G33220 AT4G33220
fragaria_vesca	FvH4_1g09610
malus_domestica	MD02G1104600.v1.1 MD02G1105100.v1.1 MD15G1224900.v1.1
prunus_persica	Prupe.7G190300_v2.0.a1
pyrus_communis	pycom02g08260
rosa_chinensis	RchiOBHm_Chr2g0096461
rosa_laevigata	RLG00000016641
rosa_multiflora	Rmu_co8263641.1_g000001 Rmu_sc0004096.1_g000019 Rmu_sc0029740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00145620
rosa_rugosa	Rorug02G0057900
rosa_samantha	Rh2AG104800 Rh2BG107000 Rh2CG108800 Rh2DG108000
rosa_wichuraiana	Rw2G008100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	608
AccB1I	GGYRCC	2 cut(s)	407, 608
AccBSI	CCGCTC	1 cut(s)	1382
AccI	GTMKAC	1 cut(s)	1433
AciI	CCGC	4 cut(s)	670, 822, 1325, 1382
AclI	AACGTT	1 cut(s)	869
AclWI	GGATC	2 cut(s)	813, 1072
AcsI	RAATTY	3 cut(s)	128, 616, 790
AfaI	GTAC	2 cut(s)	610, 1361
AfeI	AGCGCT	1 cut(s)	364
AfiI	CCNNNNNNNGG	6 cut(s)	304, 535, 919, 1131, 1382, 1496
AflII	CTTAAG	1 cut(s)	142
AgsI	TTSAA	3 cut(s)	112, 1257, 1472
AjnI	CCWGG	3 cut(s)	378, 900, 1375
AleI	CACNNNNGTG	1 cut(s)	485
AloI	GAACNNNNNNTCC	2 cut(s)	322, 354
AluBI	AGCT	4 cut(s)	16, 75, 274, 1249
AluI	AGCT	4 cut(s)	16, 75, 274, 1249
Alw21I	GWGCWC	2 cut(s)	492, 1343
Alw26I	GTCTC	4 cut(s)	288, 439, 855, 1470
Alw44I	GTGCAC	1 cut(s)	488
AlwI	GGATC	2 cut(s)	813, 1072
Ama87I	CYCGRG	1 cut(s)	1313
Aor51HI	AGCGCT	1 cut(s)	364
ApaLI	GTGCAC	1 cut(s)	488
ApeKI	GCWGC	6 cut(s)	16, 75, 78, 166, 365, 647
ApoI	RAATTY	3 cut(s)	128, 616, 790
ArsI	GACNNNNNNTTYG	6 cut(s)	417, 449, 854, 886, 1383, 1415
Asp718I	GGTACC	1 cut(s)	608
AspLEI	GCGC	3 cut(s)	365, 652, 996
AspS9I	GGNCC	7 cut(s)	512, 807, 887, 1123, 1138, 1373, 1407
AsuC2I	CCSGG	4 cut(s)	305, 613, 1397, 1410
AsuHPI	GGTGA	3 cut(s)	472, 497, 1058
AsuII	TTCGAA	1 cut(s)	402
AsuNHI	GCTAGC	1 cut(s)	71
AvaI	CYCGRG	1 cut(s)	1313
AvaII	GGWCC	2 cut(s)	807, 1138
AxyI	CCTNAGG	1 cut(s)	1302
BaeGI	GKGCMC	1 cut(s)	492
BanI	GGYRCC	2 cut(s)	407, 608
BanII	GRGCYC	1 cut(s)	1389
Bbv12I	GWGCWC	2 cut(s)	492, 1343
BbvI	GCAGC	6 cut(s)	3, 62, 90, 178, 352, 659
BccI	CCATC	4 cut(s)	389, 599, 749, 794
BceAI	ACGGC	3 cut(s)	623, 897, 1137
BcgI	CGANNNNNNTGC	2 cut(s)	1304, 1338
BciT130I	CCWGG	3 cut(s)	380, 902, 1377
BcnI	CCSGG	4 cut(s)	305, 613, 1397, 1410
BcoDI	GTCTC	4 cut(s)	288, 439, 855, 1470
BfaI	CTAG	3 cut(s)	29, 72, 1082
BfmI	CTRYAG	4 cut(s)	76, 943, 1185, 1455
BfoI	RGCGCY	2 cut(s)	366, 653
BfrI	CTTAAG	1 cut(s)	142
BisI	GCNGC	6 cut(s)	17, 76, 79, 167, 366, 648
BlsI	GCNGC	6 cut(s)	18, 77, 80, 168, 367, 649
Bme1390I	CCNGG	7 cut(s)	305, 380, 613, 902, 1377, 1397, 1410
Bme18I	GGWCC	2 cut(s)	807, 1138
BmeT110I	CYCGRG	1 cut(s)	1313
BmgT120I	GGNCC	7 cut(s)	512, 807, 887, 1123, 1138, 1373, 1407
BmiI	GGNNCC	5 cut(s)	139, 409, 610, 737, 1140
BmrFI	CCNGG	7 cut(s)	305, 380, 613, 902, 1377, 1397, 1410
BmsI	GCATC	8 cut(s)	90, 131, 577, 625, 754, 938, 1039, 1180
BmtI	GCTAGC	1 cut(s)	75
BplI	GAGNNNNNCTC	2 cut(s)	1371, 1403
BpmI	CTGGAG	1 cut(s)	1398
Bpu14I	TTCGAA	1 cut(s)	402
BpuEI	CTTGAG	2 cut(s)	1303, 1422
BpuMI	CCSGG	4 cut(s)	305, 613, 1397, 1410
BsaI	GGTCTC	4 cut(s)	288, 439, 855, 1470
BsaJI	CCNNGG	5 cut(s)	469, 508, 1126, 1231, 1241
BsaWI	WCCGGW	1 cut(s)	1154
Bsc4I	CCNNNNNNNGG	6 cut(s)	304, 535, 919, 1131, 1382, 1496
Bse118I	RCCGGY	1 cut(s)	569
Bse1I	ACTGG	6 cut(s)	284, 375, 445, 997, 1168, 1501
Bse21I	CCTNAGG	1 cut(s)	1302
Bse3DI	GCAATG	2 cut(s)	996, 1294
BseBI	CCWGG	3 cut(s)	380, 902, 1377
BseDI	CCNNGG	5 cut(s)	469, 508, 1126, 1231, 1241
BseGI	GGATG	6 cut(s)	122, 592, 640, 661, 669, 769
BseLI	CCNNNNNNNGG	6 cut(s)	304, 535, 919, 1131, 1382, 1496
BseMI	GCAATG	2 cut(s)	996, 1294
BseMII	CTCAG	3 cut(s)	203, 338, 1293
BseNI	ACTGG	6 cut(s)	284, 375, 445, 997, 1168, 1501
BseRI	GAGGAG	1 cut(s)	467
BseSI	GKGCMC	1 cut(s)	492
BseXI	GCAGC	6 cut(s)	3, 62, 90, 178, 352, 659
BsgI	GTGCAG	2 cut(s)	185, 351
Bsh1285I	CGRYCG	1 cut(s)	1155
BshNI	GGYRCC	2 cut(s)	407, 608
BsiEI	CGRYCG	1 cut(s)	1155
BsiHKAI	GWGCWC	2 cut(s)	492, 1343
BsiHKCI	CYCGRG	1 cut(s)	1313
BsiSI	CCGG	8 cut(s)	305, 570, 612, 653, 1132, 1155, 1396, 1410
BslFI	GGGAC	1 cut(s)	161
BslI	CCNNNNNNNGG	6 cut(s)	304, 535, 919, 1131, 1382, 1496
BsmAI	GTCTC	4 cut(s)	288, 439, 855, 1470
BsmFI	GGGAC	1 cut(s)	161
BsmI	GAATGC	1 cut(s)	1327
Bso31I	GGTCTC	4 cut(s)	288, 439, 855, 1470
BsoBI	CYCGRG	1 cut(s)	1313
Bsp119I	TTCGAA	1 cut(s)	402
Bsp1286I	GDGCHC	3 cut(s)	492, 1343, 1389
Bsp143I	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
BspACI	CCGC	4 cut(s)	670, 822, 1325, 1382
BspCNI	CTCAG	3 cut(s)	202, 339, 1294
BspLI	GGNNCC	5 cut(s)	139, 409, 610, 737, 1140
BspMAI	CTGCAG	2 cut(s)	80, 1189
BspOI	GCTAGC	1 cut(s)	75
BspPI	GGATC	2 cut(s)	813, 1072
BspT104I	TTCGAA	1 cut(s)	402
BspT107I	GGYRCC	2 cut(s)	407, 608
BspTI	CTTAAG	1 cut(s)	142
BspTNI	GGTCTC	4 cut(s)	288, 439, 855, 1470
BsrBI	CCGCTC	1 cut(s)	1382
BsrDI	GCAATG	2 cut(s)	996, 1294
BsrFI	RCCGGY	1 cut(s)	569
BsrI	ACTGG	6 cut(s)	284, 375, 445, 997, 1168, 1501
BssAI	RCCGGY	1 cut(s)	569
BssECI	CCNNGG	5 cut(s)	469, 508, 1126, 1231, 1241
BssMI	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
BssT1I	CCWWGG	4 cut(s)	469, 1126, 1231, 1241
Bst2UI	CCWGG	3 cut(s)	380, 902, 1377
Bst4CI	ACNGT	5 cut(s)	99, 1030, 1057, 1264, 1456
BstAFI	CTTAAG	1 cut(s)	142
BstAPI	GCANNNNNTGC	1 cut(s)	830
BstBI	TTCGAA	1 cut(s)	402
BstC8I	GCNNGC	4 cut(s)	46, 73, 120, 136
BstDEI	CTNAG	5 cut(s)	189, 335, 347, 914, 1302
BstDSI	CCRYGG	1 cut(s)	508
BstF5I	GGATG	6 cut(s)	122, 592, 640, 661, 669, 769
BstH2I	RGCGCY	2 cut(s)	366, 653
BstHHI	GCGC	3 cut(s)	365, 652, 996
BstKTI	GATC	7 cut(s)	346, 505, 553, 720, 821, 920, 1080
BstMAI	GTCTC	4 cut(s)	288, 439, 855, 1470
BstMBI	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
BstMCI	CGRYCG	1 cut(s)	1155
BstNI	CCWGG	3 cut(s)	380, 902, 1377
BstNSI	RCATGY	1 cut(s)	390
BstSCI	CCNGG	7 cut(s)	303, 378, 611, 900, 1375, 1395, 1408
BstSFI	CTRYAG	4 cut(s)	76, 943, 1185, 1455
BstSLI	GKGCMC	1 cut(s)	492
BstV1I	GCAGC	6 cut(s)	3, 62, 90, 178, 352, 659
BstX2I	RGATCY	1 cut(s)	1077
BstYI	RGATCY	1 cut(s)	1077
Bsu36I	CCTNAGG	1 cut(s)	1302
BtgI	CCRYGG	1 cut(s)	508
BtsCI	GGATG	6 cut(s)	122, 592, 640, 661, 669, 769
BtsI	GCAGTG	1 cut(s)	397
BtsIMutI	CAGTG	2 cut(s)	368, 397
Cac8I	GCNNGC	4 cut(s)	46, 73, 120, 136
CfoI	GCGC	3 cut(s)	365, 652, 996
Cfr10I	RCCGGY	1 cut(s)	569
Cfr13I	GGNCC	7 cut(s)	512, 807, 887, 1123, 1138, 1373, 1407
CseI	GACGC	1 cut(s)	1105
Csp6I	GTAC	2 cut(s)	609, 1360
CspCI	CAANNNNNGTGG	2 cut(s)	1003, 1038
CviAII	CATG	4 cut(s)	354, 387, 631, 1363
CviQI	GTAC	2 cut(s)	609, 1360
DdeI	CTNAG	5 cut(s)	189, 335, 347, 914, 1302
DpnI	GATC	7 cut(s)	345, 504, 552, 719, 820, 919, 1079
DpnII	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
Eco130I	CCWWGG	4 cut(s)	469, 1126, 1231, 1241
Eco147I	AGGCCT	4 cut(s)	474, 1095, 1240, 1300
Eco24I	GRGCYC	1 cut(s)	1389
Eco31I	GGTCTC	4 cut(s)	288, 439, 855, 1470
Eco47I	GGWCC	2 cut(s)	807, 1138
Eco47III	AGCGCT	1 cut(s)	364
Eco81I	CCTNAGG	1 cut(s)	1302
Eco88I	CYCGRG	1 cut(s)	1313
EcoO109I	RGGNCCY	1 cut(s)	1138
EcoRII	CCWGG	3 cut(s)	378, 900, 1375
EcoT14I	CCWWGG	4 cut(s)	469, 1126, 1231, 1241
EcoT38I	GRGCYC	1 cut(s)	1389
ErhI	CCWWGG	4 cut(s)	469, 1126, 1231, 1241
FaeI	CATG	4 cut(s)	357, 390, 634, 1366
FaqI	GGGAC	1 cut(s)	161
FatI	CATG	4 cut(s)	353, 386, 630, 1362
FauI	CCCGC	2 cut(s)	677, 1375
FblI	GTMKAC	1 cut(s)	1433
Fnu4HI	GCNGC	6 cut(s)	17, 76, 79, 167, 366, 648
FokI	GGATG	6 cut(s)	109, 599, 647, 668, 676, 776
FriOI	GRGCYC	1 cut(s)	1389
Fsp4HI	GCNGC	6 cut(s)	17, 76, 79, 167, 366, 648
FspBI	CTAG	3 cut(s)	29, 72, 1082
GlaI	GCGC	3 cut(s)	364, 651, 995
GluI	GCNGC	6 cut(s)	17, 76, 79, 167, 366, 648
GsuI	CTGGAG	1 cut(s)	1398
HaeII	RGCGCY	2 cut(s)	366, 653
HapII	CCGG	8 cut(s)	305, 570, 612, 653, 1132, 1155, 1396, 1410
HgaI	GACGC	1 cut(s)	1105
HhaI	GCGC	3 cut(s)	365, 652, 996
Hin1II	CATG	4 cut(s)	357, 390, 634, 1366
Hin6I	GCGC	3 cut(s)	363, 650, 994
HinP1I	GCGC	3 cut(s)	363, 650, 994
HincII	GTYRAC	2 cut(s)	376, 1213
HindII	GTYRAC	2 cut(s)	376, 1213
HinfI	GANTC	4 cut(s)	60, 923, 1158, 1430
HpaI	GTTAAC	1 cut(s)	376
HpaII	CCGG	8 cut(s)	305, 570, 612, 653, 1132, 1155, 1396, 1410
HphI	GGTGA	3 cut(s)	472, 497, 1058
Hpy166II	GTNNAC	4 cut(s)	376, 490, 1213, 1434
Hpy188I	TCNGA	8 cut(s)	103, 127, 238, 343, 348, 922, 928, 1107
Hpy188III	TCNNGA	7 cut(s)	688, 721, 780, 874, 1257, 1282, 1444
Hpy8I	GTNNAC	4 cut(s)	376, 490, 1213, 1434
HpyAV	CCTTC	6 cut(s)	302, 398, 540, 1129, 1288, 1466
HpyCH4III	ACNGT	5 cut(s)	99, 1030, 1057, 1264, 1456
HpyCH4IV	ACGT	2 cut(s)	711, 869
HpyF3I	CTNAG	5 cut(s)	189, 335, 347, 914, 1302
HpySE526I	ACGT	2 cut(s)	711, 869
Hsp92II	CATG	4 cut(s)	357, 390, 634, 1366
HspAI	GCGC	3 cut(s)	363, 650, 994
KpnI	GGTACC	1 cut(s)	612
KspAI	GTTAAC	1 cut(s)	376
Kzo9I	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
LmnI	GCTCC	3 cut(s)	53, 143, 1379
Lsp1109I	GCAGC	6 cut(s)	3, 62, 90, 178, 352, 659
LweI	GCATC	8 cut(s)	90, 131, 577, 625, 754, 938, 1039, 1180
MaeI	CTAG	3 cut(s)	29, 72, 1082
MaeII	ACGT	2 cut(s)	711, 869
MaeIII	GTNAC	3 cut(s)	454, 1117, 1200
MalI	GATC	7 cut(s)	345, 504, 552, 719, 820, 919, 1079
MbiI	CCGCTC	1 cut(s)	1382
MboI	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
MboII	GAAGA	4 cut(s)	560, 736, 739, 1120
MflI	RGATCY	1 cut(s)	1077
MhlI	GDGCHC	3 cut(s)	492, 1343, 1389
MluCI	AATT	5 cut(s)	128, 616, 790, 1366, 1463
MlyI	GAGTC	1 cut(s)	1439
MmeI	TCCRAC	4 cut(s)	249, 261, 785, 951
MseI	TTAA	4 cut(s)	143, 375, 540, 1425
MslI	CAYNNNNRTG	1 cut(s)	485
MspCI	CTTAAG	1 cut(s)	142
MspI	CCGG	8 cut(s)	305, 570, 612, 653, 1132, 1155, 1396, 1410
MspR9I	CCNGG	7 cut(s)	305, 380, 613, 902, 1377, 1397, 1410
Mva1269I	GAATGC	1 cut(s)	1327
MvaI	CCWGG	3 cut(s)	380, 902, 1377
NciI	CCSGG	4 cut(s)	305, 613, 1397, 1410
NdeII	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
NheI	GCTAGC	1 cut(s)	71
NlaIII	CATG	4 cut(s)	357, 390, 634, 1366
NlaIV	GGNNCC	5 cut(s)	139, 409, 610, 737, 1140
NmuCI	GTSAC	1 cut(s)	1117
NspI	RCATGY	1 cut(s)	390
NspV	TTCGAA	1 cut(s)	402
OliI	CACNNNNGTG	1 cut(s)	485
PaeR7I	CTCGAG	1 cut(s)	1313
PceI	AGGCCT	4 cut(s)	474, 1095, 1240, 1300
PcsI	WCGNNNNNNNCGW	1 cut(s)	234
PctI	GAATGC	1 cut(s)	1327
PfeI	GAWTC	3 cut(s)	60, 923, 1158
PkrI	GCNGC	6 cut(s)	18, 77, 80, 168, 367, 649
PleI	GAGTC	1 cut(s)	1438
PpsI	GAGTC	1 cut(s)	1438
PpuMI	RGGWCCY	1 cut(s)	1138
Psp1406I	AACGTT	1 cut(s)	869
Psp5II	RGGWCCY	1 cut(s)	1138
Psp6I	CCWGG	3 cut(s)	378, 900, 1375
PspGI	CCWGG	3 cut(s)	378, 900, 1375
PspN4I	GGNNCC	5 cut(s)	139, 409, 610, 737, 1140
PspPI	GGNCC	7 cut(s)	512, 807, 887, 1123, 1138, 1373, 1407
PspPPI	RGGWCCY	1 cut(s)	1138
PspXI	VCTCGAGB	1 cut(s)	1313
PstI	CTGCAG	2 cut(s)	80, 1189
PsuI	RGATCY	1 cut(s)	1077
RsaI	GTAC	2 cut(s)	610, 1361
RsaNI	GTAC	2 cut(s)	609, 1360
RseI	CAYNNNNRTG	1 cut(s)	485
SaqAI	TTAA	4 cut(s)	143, 375, 540, 1425
SatI	GCNGC	6 cut(s)	17, 76, 79, 167, 366, 648
Sau3AI	GATC	7 cut(s)	343, 502, 550, 717, 818, 917, 1077
Sau96I	GGNCC	7 cut(s)	512, 807, 887, 1123, 1138, 1373, 1407
SchI	GAGTC	1 cut(s)	1439
ScrFI	CCNGG	7 cut(s)	305, 380, 613, 902, 1377, 1397, 1410
SduI	GDGCHC	3 cut(s)	492, 1343, 1389
SfaNI	GCATC	8 cut(s)	90, 131, 577, 625, 754, 938, 1039, 1180
SfcI	CTRYAG	4 cut(s)	76, 943, 1185, 1455
Sfr274I	CTCGAG	1 cut(s)	1313
SfuI	TTCGAA	1 cut(s)	402
SinI	GGWCC	2 cut(s)	807, 1138
SlaI	CTCGAG	1 cut(s)	1313
SmiMI	CAYNNNNRTG	1 cut(s)	485
SmlI	CTYRAG	4 cut(s)	142, 1282, 1313, 1437
SmoI	CTYRAG	4 cut(s)	142, 1282, 1313, 1437
Sse9I	AATT	5 cut(s)	128, 616, 790, 1366, 1463
SseBI	AGGCCT	4 cut(s)	474, 1095, 1240, 1300
SsiI	CCGC	4 cut(s)	670, 822, 1325, 1382
SspMI	CTAG	3 cut(s)	29, 72, 1082
StuI	AGGCCT	4 cut(s)	474, 1095, 1240, 1300
StyD4I	CCNGG	7 cut(s)	303, 378, 611, 900, 1375, 1395, 1408
StyI	CCWWGG	4 cut(s)	469, 1126, 1231, 1241
TaaI	ACNGT	5 cut(s)	99, 1030, 1057, 1264, 1456
TaiI	ACGT	2 cut(s)	714, 872
TaqI	TCGA	9 cut(s)	246, 402, 553, 787, 798, 817, 873, 1314, 1389
TasI	AATT	5 cut(s)	128, 616, 790, 1366, 1463
TatI	WGTACW	1 cut(s)	1359
TfiI	GAWTC	3 cut(s)	60, 923, 1158
Tru1I	TTAA	4 cut(s)	143, 375, 540, 1425
Tru9I	TTAA	4 cut(s)	143, 375, 540, 1425
TscAI	CASTG	2 cut(s)	375, 397
TseFI	GTSAC	1 cut(s)	1117
TseI	GCWGC	6 cut(s)	16, 75, 78, 166, 365, 647
Tsp45I	GTSAC	1 cut(s)	1117
TspDTI	ATGAA	4 cut(s)	672, 841, 1027, 1379
TspRI	CASTG	2 cut(s)	375, 397
Vha464I	CTTAAG	1 cut(s)	142
VneI	GTGCAC	1 cut(s)	488
VpaK11BI	GGWCC	2 cut(s)	807, 1138
XapI	RAATTY	3 cut(s)	128, 616, 790
XceI	RCATGY	1 cut(s)	390
XcmI	CCANNNNNNNNNTGG	1 cut(s)	515
XhoI	CTCGAG	1 cut(s)	1313
XmiI	GTMKAC	1 cut(s)	1433
XspI	CTAG	3 cut(s)	29, 72, 1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.