Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009744

Physical Location & Seq

Genomic Coordinates Reverse (-)

8422783 .. 8425756

Gene Structure 2974 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1104600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGCTTCTGGGAAACTGCTTTTTTTTATAGTCTTATTGTGTGTCTGCTGCAAAAGCAGAATCCCTTTCGCTAGCTGCAGCTTCACAGATGACATACAGTCAGAGTGCTTGAAAGTGCCTGCATCCGAATTTGCAGGCTCGTTGAGGGACACCATTGATGCTGTGCAGCAAGTGGTCACCATACTCTCTCAGTTTGCAAATGCTTTCAGCGATTTTCGCCTTGCCAACGCCATTTCCGACTGCCTTGATTTGCTTGACTTCTCTGCTGATGAGTTGAACTGGTCCCTTTCTGCTTCCCAGAACCAGAAAGGCAAAAGTAACAGCACAGGGAAACTAAGTTCTGATCTGAGGACATGGTTGAGCGCTGCACTAGTTAACCAGGACACATGCGGTGACGGGTTTGAAGGCACCAACAGCATTGTCAAGGGGTTGGTCTCAACTGGCCTCAACCAAGTTACCTCCTTAGTCCAAGACCTCCTCACTCAAGTGCACCCGAATTCCGATCACCAGGGTCCTAACGGTCAAATCCCAACGTGGGTTAAAACCGAAGATCGAAAACTTCTACAAGCCGACGGTGTCAATGTGGATGCAGTTGTAGCCCAAGATGGTACCGGGAATTTCACAAATGTGATGGATGCCGTGTTAGCAGCGCCGGATTACAGCATGAGCCGCTACGTAATATACATCAAGAGAGGCACATATAAAGAGAATGTGGAGATCAAGAAGAAAAAATGGAACCTAATGATGATTGGAGATGGAATGGATGCTACCATAATCTCTGGAAATCGAAGCTATATCGATGGTTGGACCACATTTCGATCTGCAACATTTGCTGTGAGTGGCAGAGGGTTCATAGCAAGAGACATAACGTTCGAAAACACGGCAGGGCCACAGAAGCACCAGGCAGTAGCCCTAAGATCGGATTCGGACCTATCGGTGTTCTACAGATGCGAAATAAGAGGCTACCAAGACACACTCTACGCACACACAATGCGCCAGTTCTACAGAGATTGCAAAATAAGCGGTACAGTTGACTTTATTTTTGGTGATGCAACTGTTGTGTTCCAAAACTGTCAGATCCTAGCCAAAAAAGGCCTACCGAATCAGAAGAACAGCGTCACAGCCCAAGGCCGGAAAGACCCCAATGAGCCGACCGGAATCTCAATCCAGTTCTGCAACATAACTGCAGATGCAGACTTGTTACCCTTTGTCAACTCCACTTTTACTTACCTTGGTAGGCCTTGGAAGCTGTATTCAAGAACTGTAATAATGCAGTCTTTCTTGAGCAATGCCGTAAGGCCCGAGGGGTGGCTTGAATGGAATGCGGATTTTGCTTTGAGCACATTGTCTTATGGTGAGTACATGAATTATGGGCCTGGAGCGGGCCTCGGAAGCCGGATCAAGTGGCCTGGCTACCGAGTCTTTAATGAGTCTACTCAAGTAATGAACTATACTGTGGCCCAATTTATTGAAGGCAACCTGTGGTTACCCCCTACTGGTGTCAAATATACTGCAGGATTTGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030312 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

510

Amino Acids

56.21

Weight (kDa)

5.73

Isoelectric Point (pI)

23.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	48 - 156	2.5e-17	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	196 - 493	1.4e-147	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43270 AT4G33220 AT4G33220
fragaria_vesca	FvH4_1g09610
malus_domestica	MD02G1104600.v1.1 MD02G1105100.v1.1 MD15G1224900.v1.1
prunus_persica	Prupe.7G190300_v2.0.a1
pyrus_communis	pycom02g08260
rosa_chinensis	RchiOBHm_Chr2g0096461
rosa_laevigata	RLG00000016641
rosa_multiflora	Rmu_co8263641.1_g000001 Rmu_sc0004096.1_g000019 Rmu_sc0029740.1_g000002
rosa_roxburghii	Rroxscaffold_2G00145620
rosa_rugosa	Rorug02G0057900
rosa_samantha	Rh2AG104800 Rh2BG107000 Rh2CG108800 Rh2DG108000
rosa_wichuraiana	Rw2G008100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	608
AccB1I	GGYRCC	2 cut(s)	407, 608
AccBSI	CCGCTC	1 cut(s)	1382
AccI	GTMKAC	1 cut(s)	1433
AciI	CCGC	5 cut(s)	390, 670, 1023, 1325, 1382
AclI	AACGTT	1 cut(s)	869
AclWI	GGATC	2 cut(s)	1072, 1406
AcsI	RAATTY	3 cut(s)	128, 496, 616
AfaI	GTAC	3 cut(s)	610, 1027, 1361
AfeI	AGCGCT	1 cut(s)	364
AfiI	CCNNNNNNNGG	6 cut(s)	535, 919, 1131, 1308, 1382, 1496
AgsI	TTSAA	6 cut(s)	112, 277, 404, 1257, 1316, 1472
AhdI	GACNNNNNGTC	1 cut(s)	97
AhlI	ACTAGT	1 cut(s)	370
AjnI	CCWGG	5 cut(s)	378, 507, 900, 1375, 1408
AleI	CACNNNNGTG	2 cut(s)	485, 626
AloI	GAACNNNNNNTCC	2 cut(s)	322, 354
AluBI	AGCT	4 cut(s)	75, 81, 792, 1249
AluI	AGCT	4 cut(s)	75, 81, 792, 1249
Alw21I	GWGCWC	2 cut(s)	492, 1343
Alw26I	GTCTC	2 cut(s)	439, 855
Alw44I	GTGCAC	1 cut(s)	488
AlwI	GGATC	2 cut(s)	1072, 1406
Ama87I	CYCGRG	1 cut(s)	1301
Aor51HI	AGCGCT	1 cut(s)	364
ApaLI	GTGCAC	1 cut(s)	488
ApeKI	GCWGC	6 cut(s)	48, 75, 78, 166, 365, 647
ApoI	RAATTY	3 cut(s)	128, 496, 616
ArsI	GACNNNNNNTTYG	4 cut(s)	854, 886, 1025, 1057
Asp718I	GGTACC	1 cut(s)	608
AspLEI	GCGC	3 cut(s)	365, 652, 996
AspS9I	GGNCC	9 cut(s)	282, 512, 807, 887, 928, 1299, 1373, 1384, 1459
AsuC2I	CCSGG	1 cut(s)	613
AsuHPI	GGTGA	5 cut(s)	169, 404, 497, 1058, 1367
AsuII	TTCGAA	1 cut(s)	873
AsuNHI	GCTAGC	1 cut(s)	71
AvaI	CYCGRG	1 cut(s)	1301
AvaII	GGWCC	4 cut(s)	282, 512, 807, 928
BaeGI	GKGCMC	1 cut(s)	492
BanI	GGYRCC	2 cut(s)	407, 608
Bbv12I	GWGCWC	2 cut(s)	492, 1343
BbvI	GCAGC	6 cut(s)	35, 62, 90, 178, 352, 659
BccI	CCATC	4 cut(s)	599, 625, 749, 794
BceAI	ACGGC	3 cut(s)	623, 897, 1277
BciT130I	CCWGG	5 cut(s)	380, 509, 902, 1377, 1410
BcnI	CCSGG	1 cut(s)	613
BcoDI	GTCTC	2 cut(s)	439, 855
BcuI	ACTAGT	1 cut(s)	370
BfaI	CTAG	3 cut(s)	72, 371, 1082
BfmI	CTRYAG	5 cut(s)	76, 943, 1003, 1185, 1512
BfoI	RGCGCY	2 cut(s)	366, 653
BisI	GCNGC	7 cut(s)	49, 76, 79, 167, 366, 648, 670
BlsI	GCNGC	7 cut(s)	50, 77, 80, 168, 367, 649, 671
Bme1390I	CCNGG	6 cut(s)	380, 509, 613, 902, 1377, 1410
Bme18I	GGWCC	4 cut(s)	282, 512, 807, 928
BmeRI	GACNNNNNGTC	1 cut(s)	97
BmeT110I	CYCGRG	1 cut(s)	1301
BmgT120I	GGNCC	9 cut(s)	282, 512, 807, 887, 928, 1299, 1373, 1384, 1459
BmiI	GGNNCC	5 cut(s)	284, 409, 513, 610, 737
BmrFI	CCNGG	6 cut(s)	380, 509, 613, 902, 1377, 1410
BmsI	GCATC	8 cut(s)	131, 148, 577, 625, 754, 938, 1039, 1180
BmtI	GCTAGC	1 cut(s)	75
BplI	GAGNNNNNCTC	2 cut(s)	1371, 1403
BpmI	CTGGAG	1 cut(s)	1398
Bpu14I	TTCGAA	1 cut(s)	873
BpuEI	CTTGAG	3 cut(s)	468, 1303, 1422
BpuMI	CCSGG	1 cut(s)	613
Bsa29I	ATCGAT	1 cut(s)	798
BsaAI	YACGTR	1 cut(s)	676
BsaI	GGTCTC	1 cut(s)	439
BsaJI	CCNNGG	6 cut(s)	508, 1126, 1231, 1241, 1302, 1387
BsaWI	WCCGGW	1 cut(s)	1154
Bsc4I	CCNNNNNNNGG	6 cut(s)	535, 919, 1131, 1308, 1382, 1496
Bse1I	ACTGG	5 cut(s)	284, 445, 997, 1168, 1501
Bse3DI	GCAATG	1 cut(s)	1294
BseBI	CCWGG	5 cut(s)	380, 509, 902, 1377, 1410
BseCI	ATCGAT	1 cut(s)	798
BseDI	CCNNGG	6 cut(s)	508, 1126, 1231, 1241, 1302, 1387
BseGI	GGATG	4 cut(s)	122, 592, 640, 769
BseLI	CCNNNNNNNGG	6 cut(s)	535, 919, 1131, 1308, 1382, 1496
BseMI	GCAATG	1 cut(s)	1294
BseMII	CTCAG	2 cut(s)	203, 338
BseNI	ACTGG	5 cut(s)	284, 445, 997, 1168, 1501
BseRI	GAGGAG	1 cut(s)	467
BseSI	GKGCMC	1 cut(s)	492
BseXI	GCAGC	6 cut(s)	35, 62, 90, 178, 352, 659
BsgI	GTGCAG	2 cut(s)	185, 351
Bsh1285I	CGRYCG	1 cut(s)	1155
BshNI	GGYRCC	2 cut(s)	407, 608
BshVI	ATCGAT	1 cut(s)	798
BsiEI	CGRYCG	1 cut(s)	1155
BsiHKAI	GWGCWC	2 cut(s)	492, 1343
BsiHKCI	CYCGRG	1 cut(s)	1301
BsiSI	CCGG	5 cut(s)	612, 653, 1132, 1155, 1396
BslFI	GGGAC	2 cut(s)	161, 268
BslI	CCNNNNNNNGG	6 cut(s)	535, 919, 1131, 1308, 1382, 1496
BsmAI	GTCTC	2 cut(s)	439, 855
BsmFI	GGGAC	2 cut(s)	161, 268
BsmI	GAATGC	1 cut(s)	1327
Bso31I	GGTCTC	1 cut(s)	439
BsoBI	CYCGRG	1 cut(s)	1301
Bsp119I	TTCGAA	1 cut(s)	873
Bsp1286I	GDGCHC	2 cut(s)	492, 1343
Bsp143I	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
BspACI	CCGC	5 cut(s)	390, 670, 1023, 1325, 1382
BspCNI	CTCAG	2 cut(s)	202, 339
BspDI	ATCGAT	1 cut(s)	798
BspLI	GGNNCC	5 cut(s)	284, 409, 513, 610, 737
BspMAI	CTGCAG	3 cut(s)	80, 1189, 1516
BspOI	GCTAGC	1 cut(s)	75
BspPI	GGATC	2 cut(s)	1072, 1406
BspT104I	TTCGAA	1 cut(s)	873
BspT107I	GGYRCC	2 cut(s)	407, 608
BspTNI	GGTCTC	1 cut(s)	439
BsrBI	CCGCTC	1 cut(s)	1382
BsrDI	GCAATG	1 cut(s)	1294
BsrI	ACTGG	5 cut(s)	284, 445, 997, 1168, 1501
BssECI	CCNNGG	6 cut(s)	508, 1126, 1231, 1241, 1302, 1387
BssMI	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
BssT1I	CCWWGG	3 cut(s)	1126, 1231, 1241
Bst2UI	CCWGG	5 cut(s)	380, 509, 902, 1377, 1410
Bst4CI	ACNGT	8 cut(s)	99, 521, 575, 1030, 1057, 1073, 1264, 1456
BstAPI	GCANNNNNTGC	1 cut(s)	830
BstBAI	YACGTR	1 cut(s)	676
BstBI	TTCGAA	1 cut(s)	873
BstC8I	GCNNGC	4 cut(s)	73, 120, 136, 1384
BstDEI	CTNAG	5 cut(s)	189, 335, 347, 463, 914
BstEII	GGTNACC	2 cut(s)	175, 1485
BstF5I	GGATG	4 cut(s)	122, 592, 640, 769
BstH2I	RGCGCY	2 cut(s)	366, 653
BstHHI	GCGC	3 cut(s)	365, 652, 996
BstKTI	GATC	8 cut(s)	346, 505, 553, 720, 821, 920, 1080, 1401
BstMAI	GTCTC	2 cut(s)	439, 855
BstMBI	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
BstMCI	CGRYCG	1 cut(s)	1155
BstNI	CCWGG	5 cut(s)	380, 509, 902, 1377, 1410
BstNSI	RCATGY	1 cut(s)	390
BstPI	GGTNACC	2 cut(s)	175, 1485
BstSCI	CCNGG	6 cut(s)	378, 507, 611, 900, 1375, 1408
BstSFI	CTRYAG	5 cut(s)	76, 943, 1003, 1185, 1512
BstSLI	GKGCMC	1 cut(s)	492
BstSNI	TACGTA	1 cut(s)	676
BstV1I	GCAGC	6 cut(s)	35, 62, 90, 178, 352, 659
BstX2I	RGATCY	1 cut(s)	1077
BstYI	RGATCY	1 cut(s)	1077
Bsu15I	ATCGAT	1 cut(s)	798
BsuTUI	ATCGAT	1 cut(s)	798
BtsCI	GGATG	4 cut(s)	122, 592, 640, 769
Cac8I	GCNNGC	4 cut(s)	73, 120, 136, 1384
CfoI	GCGC	3 cut(s)	365, 652, 996
Cfr13I	GGNCC	9 cut(s)	282, 512, 807, 887, 928, 1299, 1373, 1384, 1459
ClaI	ATCGAT	1 cut(s)	798
CseI	GACGC	1 cut(s)	1105
Csp6I	GTAC	3 cut(s)	609, 1026, 1360
CviAII	CATG	4 cut(s)	354, 387, 664, 1363
CviQI	GTAC	3 cut(s)	609, 1026, 1360
DdeI	CTNAG	5 cut(s)	189, 335, 347, 463, 914
DpnI	GATC	8 cut(s)	345, 504, 552, 719, 820, 919, 1079, 1400
DpnII	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
DriI	GACNNNNNGTC	1 cut(s)	97
Eam1105I	GACNNNNNGTC	1 cut(s)	97
Eco105I	TACGTA	1 cut(s)	676
Eco130I	CCWWGG	3 cut(s)	1126, 1231, 1241
Eco147I	AGGCCT	2 cut(s)	1095, 1240
Eco31I	GGTCTC	1 cut(s)	439
Eco47I	GGWCC	4 cut(s)	282, 512, 807, 928
Eco47III	AGCGCT	1 cut(s)	364
Eco88I	CYCGRG	1 cut(s)	1301
Eco91I	GGTNACC	2 cut(s)	175, 1485
EcoO109I	RGGNCCY	1 cut(s)	512
EcoO65I	GGTNACC	2 cut(s)	175, 1485
EcoRI	GAATTC	1 cut(s)	496
EcoRII	CCWGG	5 cut(s)	378, 507, 900, 1375, 1408
EcoT14I	CCWWGG	3 cut(s)	1126, 1231, 1241
ErhI	CCWWGG	3 cut(s)	1126, 1231, 1241
FaeI	CATG	4 cut(s)	357, 390, 667, 1366
FaqI	GGGAC	2 cut(s)	161, 268
FatI	CATG	4 cut(s)	353, 386, 663, 1362
FauI	CCCGC	1 cut(s)	1375
FblI	GTMKAC	1 cut(s)	1433
Fnu4HI	GCNGC	7 cut(s)	49, 76, 79, 167, 366, 648, 670
FokI	GGATG	4 cut(s)	109, 599, 647, 776
Fsp4HI	GCNGC	7 cut(s)	49, 76, 79, 167, 366, 648, 670
FspBI	CTAG	3 cut(s)	72, 371, 1082
GlaI	GCGC	3 cut(s)	364, 651, 995
GluI	GCNGC	7 cut(s)	49, 76, 79, 167, 366, 648, 670
GsuI	CTGGAG	1 cut(s)	1398
HaeII	RGCGCY	2 cut(s)	366, 653
HapII	CCGG	5 cut(s)	612, 653, 1132, 1155, 1396
HgaI	GACGC	1 cut(s)	1105
HhaI	GCGC	3 cut(s)	365, 652, 996
Hin1II	CATG	4 cut(s)	357, 390, 667, 1366
Hin6I	GCGC	3 cut(s)	363, 650, 994
HinP1I	GCGC	3 cut(s)	363, 650, 994
HincII	GTYRAC	3 cut(s)	376, 1033, 1213
HindII	GTYRAC	3 cut(s)	376, 1033, 1213
HinfI	GANTC	6 cut(s)	60, 923, 1102, 1158, 1419, 1430
HpaI	GTTAAC	1 cut(s)	376
HpaII	CCGG	5 cut(s)	612, 653, 1132, 1155, 1396
HphI	GGTGA	5 cut(s)	169, 404, 497, 1058, 1367
Hpy166II	GTNNAC	5 cut(s)	376, 490, 1033, 1213, 1434
Hpy188III	TCNNGA	5 cut(s)	688, 721, 780, 1257, 1282
Hpy8I	GTNNAC	5 cut(s)	376, 490, 1033, 1213, 1434
Hpy99I	CGWCG	1 cut(s)	575
HpyAV	CCTTC	2 cut(s)	398, 1466
HpyCH4III	ACNGT	8 cut(s)	99, 521, 575, 1030, 1057, 1073, 1264, 1456
HpyCH4IV	ACGT	3 cut(s)	533, 675, 869
HpyF3I	CTNAG	5 cut(s)	189, 335, 347, 463, 914
HpySE526I	ACGT	3 cut(s)	533, 675, 869
Hsp92II	CATG	4 cut(s)	357, 390, 667, 1366
HspAI	GCGC	3 cut(s)	363, 650, 994
KpnI	GGTACC	1 cut(s)	612
KspAI	GTTAAC	1 cut(s)	376
Kzo9I	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
LmnI	GCTCC	1 cut(s)	1379
Lsp1109I	GCAGC	6 cut(s)	35, 62, 90, 178, 352, 659
LweI	GCATC	8 cut(s)	131, 148, 577, 625, 754, 938, 1039, 1180
MaeI	CTAG	3 cut(s)	72, 371, 1082
MaeII	ACGT	3 cut(s)	533, 675, 869
MaeIII	GTNAC	7 cut(s)	175, 317, 392, 454, 1117, 1200, 1485
MalI	GATC	8 cut(s)	345, 504, 552, 719, 820, 919, 1079, 1400
MbiI	CCGCTC	1 cut(s)	1382
MboI	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
MboII	GAAGA	3 cut(s)	560, 736, 1120
MflI	RGATCY	1 cut(s)	1077
MhlI	GDGCHC	2 cut(s)	492, 1343
MluCI	AATT	5 cut(s)	128, 496, 616, 1366, 1463
MlyI	GAGTC	2 cut(s)	1428, 1439
MmeI	TCCRAC	2 cut(s)	261, 785
MseI	TTAA	3 cut(s)	375, 540, 1425
MslI	CAYNNNNRTG	2 cut(s)	485, 626
MspI	CCGG	5 cut(s)	612, 653, 1132, 1155, 1396
MspR9I	CCNGG	6 cut(s)	380, 509, 613, 902, 1377, 1410
Mva1269I	GAATGC	1 cut(s)	1327
MvaI	CCWGG	5 cut(s)	380, 509, 902, 1377, 1410
NciI	CCSGG	1 cut(s)	613
NdeII	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
NheI	GCTAGC	1 cut(s)	71
NlaIII	CATG	4 cut(s)	357, 390, 667, 1366
NlaIV	GGNNCC	5 cut(s)	284, 409, 513, 610, 737
NmuCI	GTSAC	3 cut(s)	175, 392, 1117
NspI	RCATGY	1 cut(s)	390
NspV	TTCGAA	1 cut(s)	873
OliI	CACNNNNGTG	2 cut(s)	485, 626
PceI	AGGCCT	2 cut(s)	1095, 1240
PcsI	WCGNNNNNNNCGW	1 cut(s)	234
PctI	GAATGC	1 cut(s)	1327
PfeI	GAWTC	4 cut(s)	60, 923, 1102, 1158
PflFI	GACNNNGTC	1 cut(s)	575
PkrI	GCNGC	7 cut(s)	50, 77, 80, 168, 367, 649, 671
PleI	GAGTC	2 cut(s)	1427, 1438
PpsI	GAGTC	2 cut(s)	1427, 1438
Ppu21I	YACGTR	1 cut(s)	676
PpuMI	RGGWCCY	1 cut(s)	512
Psp1406I	AACGTT	1 cut(s)	869
Psp5II	RGGWCCY	1 cut(s)	512
Psp6I	CCWGG	5 cut(s)	378, 507, 900, 1375, 1408
PspEI	GGTNACC	2 cut(s)	175, 1485
PspGI	CCWGG	5 cut(s)	378, 507, 900, 1375, 1408
PspN4I	GGNNCC	5 cut(s)	284, 409, 513, 610, 737
PspPI	GGNCC	9 cut(s)	282, 512, 807, 887, 928, 1299, 1373, 1384, 1459
PspPPI	RGGWCCY	1 cut(s)	512
PstI	CTGCAG	3 cut(s)	80, 1189, 1516
PsuI	RGATCY	1 cut(s)	1077
PsyI	GACNNNGTC	1 cut(s)	575
RsaI	GTAC	3 cut(s)	610, 1027, 1361
RsaNI	GTAC	3 cut(s)	609, 1026, 1360
RseI	CAYNNNNRTG	2 cut(s)	485, 626
SaqAI	TTAA	3 cut(s)	375, 540, 1425
SatI	GCNGC	7 cut(s)	49, 76, 79, 167, 366, 648, 670
Sau3AI	GATC	8 cut(s)	343, 502, 550, 717, 818, 917, 1077, 1398
Sau96I	GGNCC	9 cut(s)	282, 512, 807, 887, 928, 1299, 1373, 1384, 1459
SchI	GAGTC	2 cut(s)	1428, 1439
ScrFI	CCNGG	6 cut(s)	380, 509, 613, 902, 1377, 1410
SduI	GDGCHC	2 cut(s)	492, 1343
SfaNI	GCATC	8 cut(s)	131, 148, 577, 625, 754, 938, 1039, 1180
SfcI	CTRYAG	5 cut(s)	76, 943, 1003, 1185, 1512
SfuI	TTCGAA	1 cut(s)	873
SinI	GGWCC	4 cut(s)	282, 512, 807, 928
SmiMI	CAYNNNNRTG	2 cut(s)	485, 626
SmlI	CTYRAG	3 cut(s)	483, 1282, 1437
SmoI	CTYRAG	3 cut(s)	483, 1282, 1437
SnaBI	TACGTA	1 cut(s)	676
SpeI	ACTAGT	1 cut(s)	370
Sse9I	AATT	5 cut(s)	128, 496, 616, 1366, 1463
SseBI	AGGCCT	2 cut(s)	1095, 1240
SsiI	CCGC	5 cut(s)	390, 670, 1023, 1325, 1382
SspMI	CTAG	3 cut(s)	72, 371, 1082
StuI	AGGCCT	2 cut(s)	1095, 1240
StyD4I	CCNGG	6 cut(s)	378, 507, 611, 900, 1375, 1408
StyI	CCWWGG	3 cut(s)	1126, 1231, 1241
TaaI	ACNGT	8 cut(s)	99, 521, 575, 1030, 1057, 1073, 1264, 1456
TaiI	ACGT	3 cut(s)	536, 678, 872
TaqI	TCGA	5 cut(s)	553, 787, 798, 817, 873
TasI	AATT	5 cut(s)	128, 496, 616, 1366, 1463
TatI	WGTACW	1 cut(s)	1359
TauI	GCSGC	1 cut(s)	672
TfiI	GAWTC	4 cut(s)	60, 923, 1102, 1158
Tru1I	TTAA	3 cut(s)	375, 540, 1425
Tru9I	TTAA	3 cut(s)	375, 540, 1425
TseFI	GTSAC	3 cut(s)	175, 392, 1117
TseI	GCWGC	6 cut(s)	48, 75, 78, 166, 365, 647
Tsp45I	GTSAC	3 cut(s)	175, 392, 1117
TspDTI	ATGAA	3 cut(s)	841, 1379, 1460
Tth111I	GACNNNGTC	1 cut(s)	575
VneI	GTGCAC	1 cut(s)	488
VpaK11BI	GGWCC	4 cut(s)	282, 512, 807, 928
XapI	RAATTY	3 cut(s)	128, 496, 616
XceI	RCATGY	1 cut(s)	390
XmiI	GTMKAC	1 cut(s)	1433
XspI	CTAG	3 cut(s)	72, 371, 1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.