AT4G33985

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015001
Physical Location & Seq
Reverse (-)
16288112 .. 16288971
860 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33985.1

Sequence Viewer

Length: 465 bp
ATGTCAAAACGCAACGCGCCGTTTCAAGCACCGCCTAGACCGCTAGTAAAACAACATTCATGGTCACCGGACGCGGATCGAGAAGAAGCTTGGCTTCGAAAGAAGGGGAAGCAATCATTGGGCCGACTCGGTCGGAGTAAGAGCGTGACGGACGAAGATCTAGAAGAGCTCAAGGGATGCATCGAGCTTGGATTTGGATTCGAGCCGGATTCGCCTGATTTAGATCCGAGGCTCTCGGAAACTCTTCCAGCTCTTGGTTTATACTGCGCCGTCAATAAACAGTATAGTAGCAGATTGTCACGGACTTCGTCTCTTTCGTCGATCGCATCGGAAGGTGAAAACAGCAATTCCAGCACGACGATCGTTGATCAAGGTGATGATCCGGAGACGATGAAGCTGAGGTTGAAGCAATGGGCTCAGGTGGTTGCTTGTTCGGTGAAGCAATTCTCCGGCGAACCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

154

Amino Acids

17.03

Weight (kDa)

5.91

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 49 - 107 2.7e-28 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15590 AT2G15590 AT2G15610 AT4G33985
fragaria_vesca FvH4_2g05911
malus_domestica MD05G1081800.v1.1
prunus_persica Prupe.8G126400_v2.0.a1 Prupe.8G126400_v2.0.a1
pyrus_communis pycom05g07660
rosa_chinensis RchiOBHm_Chr6g0257581
rosa_laevigata RLG00000014703
rosa_rugosa Rorug05G0570500 Rorug05G0571600
rosa_samantha Rh6AG089000 Rh6CG077600
rosa_wichuraiana Rw6G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 2 cut(s) 17, 74
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 3 cut(s) 32, 41, 74
AclWI GGATC 3 cut(s) 84, 218, 374
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 26, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 5 cut(s) 89, 169, 187, 251, 397
AluI AGCT 5 cut(s) 89, 169, 187, 251, 397
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 315, 380
AlwI GGATC 3 cut(s) 84, 218, 374
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 1 cut(s) 121
Asp700I GAANNNNTTC 2 cut(s) 243, 443
AspLEI GCGC 2 cut(s) 19, 269
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 4 cut(s) 57, 347, 386, 448
AsuII TTCGAA 1 cut(s) 97
BanII GRGCYC 2 cut(s) 171, 418
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 398
BceAI ACGGC 2 cut(s) 4, 254
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 315, 380
BfaI CTAG 3 cut(s) 36, 44, 161
BglII AGATCT 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 121
BmsI GCATC 3 cut(s) 167, 189, 335
Bpu10I CCTNAGC 2 cut(s) 398, 417
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 227
BsaWI WCCGGW 2 cut(s) 67, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 416
Bse8I GATNNNNATC 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 1 cut(s) 182
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 416
BseMII CTCAG 2 cut(s) 389, 431
Bsh1236I CGCG 2 cut(s) 17, 74
Bsh1285I CGRYCG 3 cut(s) 133, 324, 363
BshFI GGCC 1 cut(s) 123
BsiEI CGRYCG 3 cut(s) 133, 324, 363
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 4 cut(s) 68, 206, 383, 450
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 315, 380
BsmBI CGTCTC 2 cut(s) 315, 380
BsnI GGCC 1 cut(s) 123
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 171, 418
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
BspACI CCGC 3 cut(s) 32, 41, 74
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 2 cut(s) 390, 430
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 2 cut(s) 17, 74
BspPI GGATC 3 cut(s) 84, 218, 374
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
Bst4CI ACNGT 1 cut(s) 282
Bst6I CTCTTC 2 cut(s) 159, 249
BstBI TTCGAA 1 cut(s) 97
BstDEI CTNAG 2 cut(s) 398, 417
BstEII GGTNACC 1 cut(s) 63
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 2 cut(s) 17, 74
BstHHI GCGC 2 cut(s) 19, 269
BstKTI GATC 7 cut(s) 79, 160, 226, 324, 363, 370, 382
BstMAI GTCTC 2 cut(s) 315, 380
BstMBI GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
BstMCI CGRYCG 3 cut(s) 133, 324, 363
BstMWI GCNNNNNNNGC 3 cut(s) 40, 211, 351
BstPI GGTNACC 1 cut(s) 63
BstUI CGCG 2 cut(s) 17, 74
BstX2I RGATCY 2 cut(s) 157, 223
BstYI RGATCY 2 cut(s) 157, 223
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 1 cut(s) 182
CfoI GCGC 2 cut(s) 19, 269
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 80
CviAII CATG 1 cut(s) 60
DdeI CTNAG 2 cut(s) 398, 417
DpnI GATC 7 cut(s) 78, 159, 225, 323, 362, 369, 381
DpnII GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
Eam1104I CTCTTC 2 cut(s) 159, 249
EarI CTCTTC 2 cut(s) 159, 249
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 171, 418
Eco53kI GAGCTC 1 cut(s) 169
Eco91I GGTNACC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 169
EcoO65I GGTNACC 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 182
EcoT38I GRGCYC 2 cut(s) 171, 418
Esp3I CGTCTC 2 cut(s) 315, 380
FaeI CATG 1 cut(s) 63
FaiI YATR 3 cut(s) 61, 262, 285
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 367
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 2 cut(s) 171, 418
FspBI CTAG 3 cut(s) 36, 44, 161
GlaI GCGC 2 cut(s) 18, 268
HaeIII GGCC 1 cut(s) 123
HapII CCGG 4 cut(s) 68, 206, 383, 450
HgaI GACGC 1 cut(s) 80
HhaI GCGC 2 cut(s) 19, 269
Hin1II CATG 1 cut(s) 63
Hin6I GCGC 2 cut(s) 17, 267
HinP1I GCGC 2 cut(s) 17, 267
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 3 cut(s) 126, 198, 209
HpaII CCGG 4 cut(s) 68, 206, 383, 450
HphI GGTGA 4 cut(s) 57, 347, 386, 448
Hpy188I TCNGA 4 cut(s) 135, 228, 238, 331
Hpy188III TCNNGA 3 cut(s) 80, 161, 383
Hpy99I CGWCG 2 cut(s) 322, 361
HpyAV CCTTC 2 cut(s) 97, 326
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4V TGCA 1 cut(s) 180
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 211, 351
HpyF3I CTNAG 2 cut(s) 398, 417
Hsp92II CATG 1 cut(s) 63
HspAI GCGC 2 cut(s) 17, 267
Kpn2I TCCGGA 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
LguI GCTCTTC 1 cut(s) 159
LpnPI CCDG 7 cut(s) 81, 219, 228, 261, 364, 396, 404
LweI GCATC 3 cut(s) 167, 189, 335
MaeI CTAG 3 cut(s) 36, 44, 161
MaeIII GTNAC 3 cut(s) 63, 145, 297
MalI GATC 7 cut(s) 78, 159, 225, 323, 362, 369, 381
MboI GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
MboII GAAGA 4 cut(s) 95, 167, 176, 236
MflI RGATCY 2 cut(s) 157, 223
MhlI GDGCHC 2 cut(s) 171, 418
MluCI AATT 3 cut(s) 346, 443, 460
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 2 cut(s) 222, 393
Mph1103I ATGCAT 1 cut(s) 182
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 2 cut(s) 243, 443
MspI CCGG 4 cut(s) 68, 206, 383, 450
MvnI CGCG 2 cut(s) 17, 74
MwoI GCNNNNNNNGC 3 cut(s) 40, 211, 351
NdeII GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
NlaIII CATG 1 cut(s) 63
NmuCI GTSAC 3 cut(s) 63, 145, 297
NsiI ATGCAT 1 cut(s) 182
NspV TTCGAA 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PdmI GAANNNNTTC 2 cut(s) 243, 443
PfeI GAWTC 2 cut(s) 198, 209
PflFI GACNNNGTC 2 cut(s) 129, 307
PflMI CCANNNNNTGG 1 cut(s) 254
Ple19I CGATCG 2 cut(s) 324, 363
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 121
PsuI RGATCY 2 cut(s) 157, 223
PsyI GACNNNGTC 2 cut(s) 129, 307
PvuI CGATCG 2 cut(s) 324, 363
SacI GAGCTC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 159
Sau3AI GATC 7 cut(s) 76, 157, 223, 321, 360, 367, 379
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 120
SduI GDGCHC 2 cut(s) 171, 418
SetI ASST 9 cut(s) 91, 171, 189, 253, 337, 376, 399, 404, 423
SfaNI GCATC 3 cut(s) 167, 189, 335
SfuI TTCGAA 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 3 cut(s) 346, 443, 460
SsiI CCGC 3 cut(s) 32, 41, 74
SspMI CTAG 3 cut(s) 36, 44, 161
SstI GAGCTC 1 cut(s) 171
TaaI ACNGT 1 cut(s) 282
TaqI TCGA 5 cut(s) 79, 97, 183, 201, 320
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 346, 443, 460
TfiI GAWTC 2 cut(s) 198, 209
TseFI GTSAC 3 cut(s) 63, 145, 297
Tsp45I GTSAC 3 cut(s) 63, 145, 297
TspDTI ATGAA 2 cut(s) 48, 407
TspGWI ACGGA 2 cut(s) 164, 316
Tth111I GACNNNGTC 2 cut(s) 129, 307
Van91I CCANNNNNTGG 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 160
XmnI GAANNNNTTC 2 cut(s) 243, 443
XspI CTAG 3 cut(s) 36, 44, 161
Zsp2I ATGCAT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.