AT4G35470

Plant intracellular Ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006680
Physical Location & Seq
Forward (+)
16846138 .. 16849183
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35470.2

Sequence Viewer

Length: 1650 bp
ATGGATTTGATGCAAATGGATAAGCGTTTGGATTCAACGGAGCAAGTGGTTGAAGAAATCATGAGAATTCACAGATCTCTACCCGCAAGACCAGGAATTGACGAAGTTGAAGCTGCCAAGGGTTTGATTGACAACGTTGAGAAAGAAGATCAAGCTTGTTTAGAAGCCATTGCTAGACAGAGGAAAAGCTCTGAAGTTCCTGGTGAGCTTTTCATGGTGTTGCAAGAAATGAAGAAGGGTTATGTTCAGTTTCGTAGCAAAGAACAGATAAGAGAAGCTTTGAAGCTTCTCGATCTGGAATCTGTTCACTCTTTGTTCGATGATTTCATTCAAAGAGCTTCTAATTGCATTGCTTCTCCTTCCTCTAACGGCTCTGTTTCGTCTCGTCCTCCGCTGCCTCCGGCGACTACGACGGCTGCTCGTTCTGATTCTCAGTCAAGTTTGAATTTTTCTGAGAGAGCTCCCGTTCGCCCCAAGGATATGGTTTCGCGAGACGATAGCTTCGTCACAAAGTCTAAGCCTTCTTCTCTCTACAGTGATGGTTTTGCAGCTCCTCCTCGAAGACCACAGATTCTCGATTCAACACTTACCACCGGCAATGATGGAGAGAAGCTGAGTTTGATAAAACTTGCTAGTTTGATTGAGGTATCAGCTAAGAAAGCTACACAAGAGATAAATCTACAGAACAAGTTAACGGAACAACTCGAGTGGCTTCCAGATTCTCTTGGCAAGTTATCGAGTTTAACTTCGCTTGATTTGTCTGAGAATCACATTGTGGTGTTGCCAAACACTATAGGAGGACTTTCTTCCTTGACAAAACTCGATTTGCATTCCAACAGAATCGGTCAGCTTCCTGAGTCTATAGGAGAATTGTTGAACTTGGTTTACCTCAATCTTGGTAGCAACCAGTTATCATCTCTGCCTTCAGCGTTTAGTAGATTGGTTCGACTCGAGGAGCTTGATTTGAGCTGTAACAACCTCCCGATTCTCCCTGAATCCATAGGTTCGCTTGTGAGTCTAAAGAAGCTCGACGTTGAGACAAATGATATCGAAGAGATCCCATATTCTATTGGTGGATGTTCTTCTCTGATAGAACTCCGTGCAGATTATAACAAACTAAAGGCTCTTCCAGAAGCTATTGGGAAGATTACTACATTAGAGATCTTGTCTGTGCGTTACAATAACATCAGGCAGTTACCTACGACGATGTCTTCTTTAGCTAGCCTCAAAGAGCTGGATGTGAGTTTCAACGAGCTCGAGTCAGTGCCAGAGAGTTTGTGTTTTGCCACTACACTTGTGAAACTGAATATTGGAAACAATTTCGCTGACATGGTATCGCTCCCGAGATCAATAGGCAACCTTGAGATGCTTGAAGAGCTTGATATAAGCAATAATCAGATCCGTGTTCTACCTGACTCCTTCAAAATGCTTACAAAGTTGCGTGTGTTTCGTGCTCAGGAGAATCCACTACACATTCCTCCTCGTGATATAGCTGAAAAGGGCCCTCAGGCCGTTGTTCAATACATGAATGATCTTGTGGAGACGAGAAACGCGAAATCTCTAATGGTTAAGCCAAAGAAGAGCTGGGTCCAGATGTGCTTCTTCTCGAAATCCAACAAGAGAAAACAGAGCAGCATGGAGATTGTCTAA

Protein Analysis

549

Amino Acids

60.99

Weight (kDa)

5.52

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 239 - 281 6.4e-08 Leucine rich repeat
LRR_14 PF23598 240 - 324 5.4e-12 Leucine-rich repeat region
LRR_4 PF12799 247 - 285 5.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 270 - 327 2.5e-10 Leucine rich repeat
LRR_8 PF13855 293 - 350 9.7e-09 Leucine rich repeat
LRR_14 PF23598 320 - 391 1.1e-09 Leucine-rich repeat region
LRR_14 PF23598 444 - 487 1.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
AccII CGCG 2 cut(s) 490, 1553
AciI CCGC 2 cut(s) 84, 392
AclI AACGTT 1 cut(s) 135
AclWI GGATC 2 cut(s) 1051, 1393
AcsI RAATTY 2 cut(s) 66, 445
AcuI CTGAAG 2 cut(s) 213, 909
AdeI CACNNNGTG 1 cut(s) 775
AjnI CCWGG 2 cut(s) 91, 199
Alw21I GWGCWC 3 cut(s) 463, 1257, 1456
Alw26I GTCTC 4 cut(s) 387, 486, 1031, 1535
AlwI GGATC 2 cut(s) 1051, 1393
Ama87I CYCGRG 4 cut(s) 704, 950, 1256, 1342
AoxI GGCC 2 cut(s) 1501, 1509
ApaI GGGCCC 1 cut(s) 1505
ApeKI GCWGC 5 cut(s) 113, 394, 416, 548, 1632
ApoI RAATTY 2 cut(s) 66, 445
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
Asp700I GAANNNNTTC 1 cut(s) 303
AspS9I GGNCC 3 cut(s) 1501, 1502, 1588
AsuHPI GGTGA 1 cut(s) 215
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 4 cut(s) 704, 950, 1256, 1342
AvaII GGWCC 1 cut(s) 1588
AxyI CCTNAGG 1 cut(s) 1506
BaeGI GKGCMC 1 cut(s) 1505
BanII GRGCYC 3 cut(s) 463, 1257, 1505
BauI CACGAG 1 cut(s) 1482
BbsI GAAGAC 2 cut(s) 568, 1203
Bbv12I GWGCWC 3 cut(s) 463, 1257, 1456
BbvI GCAGC 5 cut(s) 100, 381, 403, 560, 1644
BccI CCATC 2 cut(s) 533, 596
BceAI ACGGC 3 cut(s) 385, 429, 1496
BciT130I CCWGG 2 cut(s) 93, 201
BcoDI GTCTC 4 cut(s) 387, 486, 1031, 1535
BfaI CTAG 3 cut(s) 174, 633, 1221
BfmI CTRYAG 4 cut(s) 532, 680, 792, 861
BglII AGATCT 2 cut(s) 74, 1161
BisI GCNGC 5 cut(s) 114, 395, 417, 549, 1633
BlsI GCNGC 5 cut(s) 115, 396, 418, 550, 1634
Bme1390I CCNGG 2 cut(s) 93, 201
Bme18I GGWCC 1 cut(s) 1588
BmeT110I CYCGRG 4 cut(s) 704, 950, 1256, 1342
BmgT120I GGNCC 3 cut(s) 1501, 1502, 1588
BmiI GGNNCC 2 cut(s) 1503, 1589
BmrFI CCNGG 2 cut(s) 93, 201
BmsI GCATC 1 cut(s) 1356
BmtI GCTAGC 1 cut(s) 1224
BpiI GAAGAC 2 cut(s) 568, 1203
Bpu10I CCTNAGC 1 cut(s) 1455
BpuEI CTTGAG 1 cut(s) 1382
BsaJI CCNNGG 2 cut(s) 117, 474
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 907
Bse21I CCTNAGG 1 cut(s) 1506
Bse3DI GCAATG 3 cut(s) 168, 348, 604
BseBI CCWGG 2 cut(s) 93, 201
BseDI CCNNGG 2 cut(s) 117, 474
BseGI GGATG 2 cut(s) 1082, 1243
BseMI GCAATG 3 cut(s) 168, 348, 604
BseMII CTCAG 7 cut(s) 444, 446, 605, 753, 846, 1469, 1520
BseNI ACTGG 1 cut(s) 907
BseRI GAGGAG 4 cut(s) 543, 546, 968, 1470
BseSI GKGCMC 1 cut(s) 1505
BseXI GCAGC 5 cut(s) 100, 381, 403, 560, 1644
BseYI CCCAGC 1 cut(s) 1584
BsgI GTGCAG 1 cut(s) 1122
Bsh1236I CGCG 2 cut(s) 490, 1553
BshFI GGCC 2 cut(s) 1503, 1511
BsiHKAI GWGCWC 3 cut(s) 463, 1257, 1456
BsiHKCI CYCGRG 4 cut(s) 704, 950, 1256, 1342
BsiSI CCGG 2 cut(s) 401, 594
BsmAI GTCTC 4 cut(s) 387, 486, 1031, 1535
BsmBI CGTCTC 3 cut(s) 387, 486, 1535
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 2 cut(s) 1503, 1511
BsoBI CYCGRG 4 cut(s) 704, 950, 1256, 1342
Bsp120I GGGCCC 1 cut(s) 1501
Bsp1286I GDGCHC 4 cut(s) 463, 1257, 1456, 1505
Bsp143I GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
Bsp68I TCGCGA 1 cut(s) 490
BspACI CCGC 2 cut(s) 84, 392
BspANI GGCC 2 cut(s) 1503, 1511
BspCNI CTCAG 7 cut(s) 445, 445, 606, 754, 847, 1468, 1519
BspFNI CGCG 2 cut(s) 490, 1553
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 1503, 1589
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 2 cut(s) 1051, 1393
BspQI GCTCTTC 3 cut(s) 1131, 1368, 1574
BsrDI GCAATG 3 cut(s) 168, 348, 604
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 907
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 2 cut(s) 117, 474
BssMI GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
BssSI CACGAG 1 cut(s) 1482
BssT1I CCWWGG 2 cut(s) 117, 474
Bst2BI CACGAG 1 cut(s) 1482
Bst2UI CCWGG 2 cut(s) 93, 201
Bst4CI ACNGT 1 cut(s) 536
Bst6I CTCTTC 4 cut(s) 1047, 1131, 1368, 1574
BstC8I GCNNGC 1 cut(s) 1222
BstDEI CTNAG 9 cut(s) 432, 453, 516, 614, 654, 762, 855, 1455, 1506
BstF5I GGATG 2 cut(s) 1082, 1243
BstFNI CGCG 2 cut(s) 490, 1553
BstKTI GATC 8 cut(s) 77, 151, 295, 1059, 1164, 1349, 1401, 1534
BstMAI GTCTC 4 cut(s) 387, 486, 1031, 1535
BstMBI GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
BstMWI GCNNNNNNNGC 2 cut(s) 659, 1375
BstNI CCWGG 2 cut(s) 93, 201
BstSCI CCNGG 2 cut(s) 91, 199
BstSFI CTRYAG 4 cut(s) 532, 680, 792, 861
BstSLI GKGCMC 1 cut(s) 1505
BstUI CGCG 2 cut(s) 490, 1553
BstV1I GCAGC 5 cut(s) 100, 381, 403, 560, 1644
BstV2I GAAGAC 2 cut(s) 568, 1203
BstX2I RGATCY 4 cut(s) 74, 1056, 1161, 1398
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 4 cut(s) 74, 1056, 1161, 1398
Bsu36I CCTNAGG 1 cut(s) 1506
BsuRI GGCC 2 cut(s) 1503, 1511
BtsCI GGATG 2 cut(s) 1082, 1243
BtsIMutI CAGTG 2 cut(s) 541, 1269
BtuMI TCGCGA 1 cut(s) 490
Cac8I GCNNGC 1 cut(s) 1222
CciI TCATGA 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 3 cut(s) 1501, 1502, 1588
CspCI CAANNNNNGTGG 4 cut(s) 689, 724, 1276, 1311
CviAII CATG 5 cut(s) 61, 214, 1330, 1525, 1636
DdeI CTNAG 9 cut(s) 432, 453, 516, 614, 654, 762, 855, 1455, 1506
DpnI GATC 8 cut(s) 76, 150, 294, 1058, 1163, 1348, 1400, 1533
DpnII GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
DraIII CACNNNGTG 1 cut(s) 775
Eam1104I CTCTTC 4 cut(s) 1047, 1131, 1368, 1574
EarI CTCTTC 4 cut(s) 1047, 1131, 1368, 1574
Ecl136II GAGCTC 2 cut(s) 461, 1255
Eco130I CCWWGG 2 cut(s) 117, 474
Eco24I GRGCYC 3 cut(s) 463, 1257, 1505
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 1 cut(s) 1588
Eco53kI GAGCTC 2 cut(s) 461, 1255
Eco57I CTGAAG 2 cut(s) 213, 909
Eco81I CCTNAGG 1 cut(s) 1506
Eco88I CYCGRG 4 cut(s) 704, 950, 1256, 1342
EcoICRI GAGCTC 2 cut(s) 461, 1255
EcoO109I RGGNCCY 2 cut(s) 1501, 1502
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 91, 199
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 2 cut(s) 117, 474
EcoT38I GRGCYC 3 cut(s) 463, 1257, 1505
ErhI CCWWGG 2 cut(s) 117, 474
Esp3I CGTCTC 3 cut(s) 387, 486, 1535
FaeI CATG 5 cut(s) 64, 217, 1333, 1528, 1639
FalI AAGNNNNNCTT 2 cut(s) 262, 294
FatI CATG 5 cut(s) 60, 213, 1329, 1524, 1635
FauI CCCGC 1 cut(s) 91
Fnu4HI GCNGC 5 cut(s) 114, 395, 417, 549, 1633
FokI GGATG 2 cut(s) 1089, 1250
FriOI GRGCYC 3 cut(s) 463, 1257, 1505
Fsp4HI GCNGC 5 cut(s) 114, 395, 417, 549, 1633
FspBI CTAG 3 cut(s) 174, 633, 1221
GluI GCNGC 5 cut(s) 114, 395, 417, 549, 1633
GsaI CCCAGC 1 cut(s) 1588
HaeIII GGCC 2 cut(s) 1503, 1511
HapII CCGG 2 cut(s) 401, 594
Hin1II CATG 5 cut(s) 64, 217, 1333, 1528, 1639
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HindIII AAGCTT 3 cut(s) 153, 276, 284
HpaI GTTAAC 1 cut(s) 693
HpaII CCGG 2 cut(s) 401, 594
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 3 cut(s) 307, 693, 886
Hpy188I TCNGA 6 cut(s) 193, 427, 454, 763, 1089, 1398
Hpy8I GTNNAC 3 cut(s) 307, 693, 886
Hpy99I CGWCG 3 cut(s) 415, 1034, 1207
HpyAV CCTTC 5 cut(s) 229, 369, 531, 933, 1429
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4IV ACGT 2 cut(s) 135, 1032
HpyCH4V TGCA 6 cut(s) 13, 223, 348, 548, 829, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 1375
HpyF3I CTNAG 9 cut(s) 432, 453, 516, 614, 654, 762, 855, 1455, 1506
HpySE526I ACGT 2 cut(s) 135, 1032
Hsp92II CATG 5 cut(s) 64, 217, 1333, 1528, 1639
KspAI GTTAAC 1 cut(s) 693
Kzo9I GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
LguI GCTCTTC 3 cut(s) 1131, 1368, 1574
LmnI GCTCC 5 cut(s) 40, 466, 556, 955, 1344
Lsp1109I GCAGC 5 cut(s) 100, 381, 403, 560, 1644
LweI GCATC 1 cut(s) 1356
MaeI CTAG 3 cut(s) 174, 633, 1221
MaeII ACGT 2 cut(s) 135, 1032
MaeIII GTNAC 4 cut(s) 505, 971, 1175, 1194
MalI GATC 8 cut(s) 76, 150, 294, 1058, 1163, 1348, 1400, 1533
MboI GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
MflI RGATCY 4 cut(s) 74, 1056, 1161, 1398
MhlI GDGCHC 4 cut(s) 463, 1257, 1456, 1505
MluCI AATT 6 cut(s) 66, 96, 343, 445, 869, 1318
MlyI GAGTC 5 cut(s) 866, 942, 1024, 1268, 1409
MmeI TCCRAC 2 cut(s) 858, 1638
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 3 cut(s) 692, 743, 1569
MslI CAYNNNNRTG 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 2 cut(s) 401, 594
MspR9I CCNGG 2 cut(s) 93, 201
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 2 cut(s) 93, 201
MvnI CGCG 2 cut(s) 490, 1553
MwoI GCNNNNNNNGC 2 cut(s) 659, 1375
NdeII GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
NheI GCTAGC 1 cut(s) 1220
NlaIII CATG 5 cut(s) 64, 217, 1333, 1528, 1639
NlaIV GGNNCC 2 cut(s) 1503, 1589
NmuCI GTSAC 1 cut(s) 505
NruI TCGCGA 1 cut(s) 490
PaeR7I CTCGAG 3 cut(s) 704, 950, 1256
PagI TCATGA 1 cut(s) 60
PciSI GCTCTTC 3 cut(s) 1131, 1368, 1574
PcsI WCGNNNNNNNCGW 2 cut(s) 501, 1550
PctI GAATGC 1 cut(s) 829
PdmI GAANNNNTTC 1 cut(s) 303
PflFI GACNNNGTC 1 cut(s) 1207
PkrI GCNGC 5 cut(s) 115, 396, 418, 550, 1634
PleI GAGTC 5 cut(s) 865, 942, 1023, 1267, 1409
PpsI GAGTC 5 cut(s) 865, 942, 1023, 1267, 1409
PsiI TTATAA 1 cut(s) 1110
Psp124BI GAGCTC 2 cut(s) 463, 1257
Psp1406I AACGTT 1 cut(s) 135
Psp6I CCWGG 2 cut(s) 91, 199
PspFI CCCAGC 1 cut(s) 1584
PspGI CCWGG 2 cut(s) 91, 199
PspN4I GGNNCC 2 cut(s) 1503, 1589
PspOMI GGGCCC 1 cut(s) 1501
PspPI GGNCC 3 cut(s) 1501, 1502, 1588
PspXI VCTCGAGB 3 cut(s) 704, 950, 1256
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PsuI RGATCY 4 cut(s) 74, 1056, 1161, 1398
PsyI GACNNNGTC 1 cut(s) 1207
RruI TCGCGA 1 cut(s) 490
RseI CAYNNNNRTG 1 cut(s) 776
SacI GAGCTC 2 cut(s) 463, 1257
SapI GCTCTTC 3 cut(s) 1131, 1368, 1574
SaqAI TTAA 3 cut(s) 692, 743, 1569
SatI GCNGC 5 cut(s) 114, 395, 417, 549, 1633
Sau3AI GATC 8 cut(s) 74, 148, 292, 1056, 1161, 1346, 1398, 1531
Sau96I GGNCC 3 cut(s) 1501, 1502, 1588
SchI GAGTC 5 cut(s) 866, 942, 1024, 1268, 1409
ScrFI CCNGG 2 cut(s) 93, 201
SduI GDGCHC 4 cut(s) 463, 1257, 1456, 1505
SfaNI GCATC 1 cut(s) 1356
SfcI CTRYAG 4 cut(s) 532, 680, 792, 861
Sfr274I CTCGAG 3 cut(s) 704, 950, 1256
SinI GGWCC 1 cut(s) 1588
SlaI CTCGAG 3 cut(s) 704, 950, 1256
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 4 cut(s) 704, 950, 1256, 1361
SmoI CTYRAG 4 cut(s) 704, 950, 1256, 1361
Sse9I AATT 6 cut(s) 66, 96, 343, 445, 869, 1318
SsiI CCGC 2 cut(s) 84, 392
SspI AATATT 1 cut(s) 1309
SspMI CTAG 3 cut(s) 174, 633, 1221
SstI GAGCTC 2 cut(s) 463, 1257
StyD4I CCNGG 2 cut(s) 91, 199
StyI CCWWGG 2 cut(s) 117, 474
TaaI ACNGT 1 cut(s) 536
TaiI ACGT 2 cut(s) 138, 1035
TaqII GACCGA 1 cut(s) 833
TasI AATT 6 cut(s) 66, 96, 343, 445, 869, 1318
Tru1I TTAA 3 cut(s) 692, 743, 1569
Tru9I TTAA 3 cut(s) 692, 743, 1569
TscAI CASTG 2 cut(s) 541, 1269
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 5 cut(s) 113, 394, 416, 548, 1632
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 4 cut(s) 202, 245, 316, 1541
TspGWI ACGGA 4 cut(s) 53, 710, 1088, 1391
TspRI CASTG 2 cut(s) 541, 1269
Tth111I GACNNNGTC 1 cut(s) 1207
VpaK11BI GGWCC 1 cut(s) 1588
XapI RAATTY 2 cut(s) 66, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 1581
XhoI CTCGAG 3 cut(s) 704, 950, 1256
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 3 cut(s) 174, 633, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.