Rroxscaffold_4G00329650

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006680
Physical Location & Seq
Forward (+)
62962117 .. 62966182
4066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329650.1

Sequence Viewer

Length: 1353 bp
ATGCCTACAGTTAGATTTGAAGTACAGTACCCGGAGGGAAGAAATAAGATGTCTCCTTCATCAGCAAGAGATACAAAGGAACATGAAATAACAGAAGATGCATCGCCTACACTTGAATCAGATGTACAGAATGTACAGGAAACAAGGGATGAGATGAAAACATCGGATTCAGCAATAGATATAGGGGAGAGGTTGAAGGGAATTGAAGGGAGTGAATTGGCAGAAGATCTATATGAAGTAAAAGGTCAAGAGGGTGAGAAATTGAGTTTGACAGAGATAGCGAGTCTAAATGAATTCATGGCCCTGAAAAGGAGGGAGGATGTTAATCTTCTGAACAAGATTATAGAGCAAATTGAAGTCTTGGCCCAGAAAGGGACTAGGGATGTTAATCTTCAAAACAAGATCACAGAGCAAATTGAATGTCTTCCTTACTTGATAGGAAAGCTTTCAAGTTTTCTCACCGTTGATTTATCAGAAAACGGGATTTTGGTCCTGCCATTCAACATTGGAGGTCTTTCCTCCTTGACCAGATTGGATTTGCATTCAAACAGAATCACTGAACTACCTGACACTATTGGAGATCTTCTTAGCCTTGTCTATCTTGACCTCAGCCGTAACATATTGACATGTCTGCCGTCTACATTTGGCCGATTGGTACACCTTGAAGAGCTCAATTTAAGCTCAAACGAGCTCTTTTCTCTTCCTGATTCAATTGGATCATTTGTCAATCTCAAGAAACTGCATGTAGACACCAATAATATTGTTGAAATTCCTCATAGCATTGGTCACTGTTCGTCCCTAAGAGAACTCCATGCAGATTTTAACCGGCTTAAAGCACTTCCTGAAGCCATAGGAAAGATTGAGGGCCTGGAGATTTTGTCAGTGCGTTACAATAACATCAAAATGTTGCCGACAACTATGTCATCTCTACTTGGATTGAAGGAACTTGATGTAAGTTTCAATGAGCTTGAGTCAGTGCCTGAGAGCTTATGTTTCGCCACCTCCCTGGTCAAGATGAACATAGGGAACAATTTCGCTGATCTGCGATCCCTGCCAAGATCTATAGGGAACCTCGAAATGCTTGAAGAGTTGGATATTAGCAATAACCAGATACGGTGTCTCCCCAACTCTTTCAGAATGCTCACACGATTACGTGTACTTCGAATAGAAGATACCCCTCTTGAACTCCCTCCTAGACATGTAGCTGAAAAGGGAGCACAAGCTGTTGTCCAATACATGGCAGAGCTTGTCGAGAAAGTTGATCCACAAACAACTAAGCAGAAAAAAGGTTGGGCTCAGATATTCTTCTCAAGGTCAAGCAAAAGGAAGAGCAATCCTTACGTCAAAGCCTGA

Protein Analysis

450

Amino Acids

50.56

Weight (kDa)

5.2

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 138 - 201 9.3e-07 Leucine-rich repeat region
LRR_8 PF13855 155 - 206 1.4e-06 Leucine rich repeat
LRR_8 PF13855 174 - 231 3e-11 Leucine rich repeat
LRR_14 PF23598 191 - 271 2.3e-11 Leucine-rich repeat region
LRR_8 PF13855 220 - 277 1.6e-07 Leucine rich repeat
LRR_8 PF13855 335 - 394 9.6e-06 Leucine rich repeat
LRR_14 PF23598 348 - 391 7e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 919
AccB7I CCANNNNNTGG 1 cut(s) 1237
AccI GTMKAC 2 cut(s) 638, 747
AclWI GGATC 3 cut(s) 724, 1041, 1256
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 2 cut(s) 293, 768
AcuI CTGAAG 1 cut(s) 864
AfaI GTAC 6 cut(s) 24, 29, 126, 135, 657, 1158
AfiI CCNNNNNNNGG 3 cut(s) 309, 372, 1237
AflIII ACRYGT 3 cut(s) 626, 1153, 1198
AjnI CCWGG 2 cut(s) 867, 1005
AluBI AGCT 9 cut(s) 445, 670, 681, 691, 967, 987, 1205, 1223, 1246
AluI AGCT 9 cut(s) 445, 670, 681, 691, 967, 987, 1205, 1223, 1246
Alw21I GWGCWC 3 cut(s) 672, 693, 1219
Alw26I GTCTC 2 cut(s) 57, 1124
AlwI GGATC 3 cut(s) 724, 1041, 1256
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 4 cut(s) 300, 363, 646, 865
ApoI RAATTY 2 cut(s) 293, 768
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
Asp700I GAANNNNTTC 3 cut(s) 423, 445, 1031
AspS9I GGNCC 4 cut(s) 301, 364, 490, 865
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 266, 451
AsuII TTCGAA 1 cut(s) 1162
AvaII GGWCC 1 cut(s) 490
BanII GRGCYC 3 cut(s) 672, 693, 1297
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 3 cut(s) 672, 693, 1219
BbvCI CCTCAGC 1 cut(s) 608
BceAI ACGGC 2 cut(s) 597, 619
BciT130I CCWGG 2 cut(s) 869, 1007
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 57, 1124
BfaI CTAG 2 cut(s) 378, 1194
BfmI CTRYAG 2 cut(s) 6, 1062
BglII AGATCT 3 cut(s) 226, 580, 1058
Bme1390I CCNGG 3 cut(s) 32, 869, 1007
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 4 cut(s) 301, 364, 490, 865
BmiI GGNNCC 1 cut(s) 1070
BmrFI CCNGG 3 cut(s) 32, 869, 1007
BmsI GCATC 2 cut(s) 88, 110
BpiI GAAGAC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 890
Bpu10I CCTNAGC 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 1162
BpuEI CTTGAG 3 cut(s) 716, 989, 1294
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 1154
BsaBI GATNNNNATC 2 cut(s) 324, 387
BsaJI CCNNGG 1 cut(s) 1005
BsaXI ACNNNNNCTCC 4 cut(s) 863, 893, 1104, 1134
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 372, 1237
Bse118I RCCGGY 1 cut(s) 825
Bse8I GATNNNNATC 2 cut(s) 324, 387
BseBI CCWGG 2 cut(s) 869, 1007
BseDI CCNNGG 1 cut(s) 1005
BseGI GGATG 3 cut(s) 154, 325, 388
BseJI GATNNNNATC 2 cut(s) 324, 387
BseLI CCNNNNNNNGG 3 cut(s) 309, 372, 1237
BseMII CTCAG 3 cut(s) 622, 972, 1310
BshFI GGCC 4 cut(s) 302, 365, 648, 867
BsiHKAI GWGCWC 3 cut(s) 672, 693, 1219
BsiSI CCGG 2 cut(s) 32, 826
BslFI GGGAC 2 cut(s) 388, 781
BslI CCNNNNNNNGG 3 cut(s) 309, 372, 1237
BsmAI GTCTC 2 cut(s) 57, 1124
BsmFI GGGAC 2 cut(s) 388, 781
BsmI GAATGC 2 cut(s) 541, 1143
BsnI GGCC 4 cut(s) 302, 365, 648, 867
Bsp119I TTCGAA 1 cut(s) 1162
Bsp1286I GDGCHC 4 cut(s) 672, 693, 1219, 1297
Bsp1407I TGTACA 2 cut(s) 124, 133
Bsp143I GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
BspANI GGCC 4 cut(s) 302, 365, 648, 867
BspCNI CTCAG 3 cut(s) 621, 973, 1309
BspLI GGNNCC 1 cut(s) 1070
BspPI GGATC 3 cut(s) 724, 1041, 1256
BspQI GCTCTTC 2 cut(s) 660, 1322
BspT104I TTCGAA 1 cut(s) 1162
BsrFI RCCGGY 1 cut(s) 825
BsrGI TGTACA 2 cut(s) 124, 133
BssAI RCCGGY 1 cut(s) 825
BssECI CCNNGG 1 cut(s) 1005
BssMI GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
Bst2UI CCWGG 2 cut(s) 869, 1007
Bst4CI ACNGT 5 cut(s) 10, 27, 463, 791, 1116
Bst6I CTCTTC 4 cut(s) 660, 705, 1080, 1322
BstAUI TGTACA 2 cut(s) 124, 133
BstBAI YACGTR 1 cut(s) 1154
BstBI TTCGAA 1 cut(s) 1162
BstDEI CTNAG 6 cut(s) 587, 608, 800, 981, 1275, 1296
BstF5I GGATG 3 cut(s) 154, 325, 388
BstKTI GATC 8 cut(s) 229, 405, 583, 719, 1042, 1049, 1061, 1264
BstMAI GTCTC 2 cut(s) 57, 1124
BstMBI GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
BstMWI GCNNNNNNNGC 1 cut(s) 1051
BstNI CCWGG 2 cut(s) 869, 1007
BstNSI RCATGY 3 cut(s) 630, 746, 1202
BstSCI CCNGG 3 cut(s) 30, 867, 1005
BstSFI CTRYAG 2 cut(s) 6, 1062
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 3 cut(s) 226, 580, 1058
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstYI RGATCY 3 cut(s) 226, 580, 1058
BsuRI GGCC 4 cut(s) 302, 365, 648, 867
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 154, 325, 388
BtsIMutI CAGTG 4 cut(s) 555, 787, 888, 981
CaiI CAGNNNCTG 1 cut(s) 980
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 4 cut(s) 301, 364, 490, 865
Csp6I GTAC 6 cut(s) 23, 28, 125, 134, 656, 1157
CviAII CATG 7 cut(s) 83, 298, 627, 743, 812, 1199, 1237
CviQI GTAC 6 cut(s) 23, 28, 125, 134, 656, 1157
DdeI CTNAG 6 cut(s) 587, 608, 800, 981, 1275, 1296
DpnI GATC 8 cut(s) 228, 404, 582, 718, 1041, 1048, 1060, 1263
DpnII GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
DrdI GACNNNNNNGTC 1 cut(s) 919
DseDI GACNNNNNNGTC 1 cut(s) 919
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 4 cut(s) 660, 705, 1080, 1322
EarI CTCTTC 4 cut(s) 660, 705, 1080, 1322
Ecl136II GAGCTC 2 cut(s) 670, 691
Eco24I GRGCYC 3 cut(s) 672, 693, 1297
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 2 cut(s) 670, 691
Eco57I CTGAAG 1 cut(s) 864
EcoICRI GAGCTC 2 cut(s) 670, 691
EcoO109I RGGNCCY 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 867, 1005
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 3 cut(s) 672, 693, 1297
FaeI CATG 7 cut(s) 86, 301, 630, 746, 815, 1202, 1240
FaqI GGGAC 2 cut(s) 388, 781
FatI CATG 7 cut(s) 82, 297, 626, 742, 811, 1198, 1236
FblI GTMKAC 2 cut(s) 638, 747
FokI GGATG 3 cut(s) 161, 332, 395
FriOI GRGCYC 3 cut(s) 672, 693, 1297
FspBI CTAG 2 cut(s) 378, 1194
GsuI CTGGAG 1 cut(s) 890
HaeIII GGCC 4 cut(s) 302, 365, 648, 867
HapII CCGG 2 cut(s) 32, 826
Hin1II CATG 7 cut(s) 86, 301, 630, 746, 815, 1202, 1240
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 6 cut(s) 116, 167, 283, 552, 707, 971
HpaII CCGG 2 cut(s) 32, 826
HphI GGTGA 2 cut(s) 266, 451
Hpy166II GTNNAC 4 cut(s) 639, 658, 748, 1157
Hpy188I TCNGA 6 cut(s) 121, 166, 333, 475, 1136, 1299
Hpy188III TCNNGA 8 cut(s) 248, 602, 704, 733, 842, 1012, 1181, 1252
Hpy8I GTNNAC 4 cut(s) 639, 658, 748, 1157
HpyAV CCTTC 4 cut(s) 66, 190, 200, 934
HpyCH4III ACNGT 5 cut(s) 10, 27, 463, 791, 1116
HpyCH4IV ACGT 2 cut(s) 1153, 1341
HpyCH4V TGCA 4 cut(s) 101, 541, 742, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 1051
HpyF3I CTNAG 6 cut(s) 587, 608, 800, 981, 1275, 1296
HpySE526I ACGT 2 cut(s) 1153, 1341
Hsp92II CATG 7 cut(s) 86, 301, 630, 746, 815, 1202, 1240
Kzo9I GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
LguI GCTCTTC 2 cut(s) 660, 1322
LmnI GCTCC 1 cut(s) 1214
LweI GCATC 2 cut(s) 88, 110
MaeI CTAG 2 cut(s) 378, 1194
MaeII ACGT 2 cut(s) 1153, 1341
MaeIII GTNAC 3 cut(s) 614, 785, 887
MalI GATC 8 cut(s) 228, 404, 582, 718, 1041, 1048, 1060, 1263
MboI GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 3 cut(s) 226, 580, 1058
MhlI GDGCHC 4 cut(s) 672, 693, 1219, 1297
MlyI GAGTC 2 cut(s) 292, 980
MmeI TCCRAC 1 cut(s) 1071
Mph1103I ATGCAT 1 cut(s) 103
MroXI GAANNNNTTC 3 cut(s) 423, 445, 1031
MseI TTAA 5 cut(s) 324, 387, 677, 822, 831
MslI CAYNNNNRTG 1 cut(s) 902
MspI CCGG 2 cut(s) 32, 826
MspR9I CCNGG 3 cut(s) 32, 869, 1007
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 2 cut(s) 541, 1143
MvaI CCWGG 2 cut(s) 869, 1007
MwoI GCNNNNNNNGC 1 cut(s) 1051
NciI CCSGG 1 cut(s) 32
NdeII GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
NlaIII CATG 7 cut(s) 86, 301, 630, 746, 815, 1202, 1240
NlaIV GGNNCC 1 cut(s) 1070
NmuCI GTSAC 1 cut(s) 785
NsiI ATGCAT 1 cut(s) 103
NspI RCATGY 3 cut(s) 630, 746, 1202
NspV TTCGAA 1 cut(s) 1162
PciI ACATGT 2 cut(s) 626, 1198
PciSI GCTCTTC 2 cut(s) 660, 1322
PcsI WCGNNNNNNNCGW 1 cut(s) 1159
PctI GAATGC 2 cut(s) 541, 1143
PdmI GAANNNNTTC 3 cut(s) 423, 445, 1031
PfeI GAWTC 4 cut(s) 116, 167, 552, 707
PflMI CCANNNNNTGG 1 cut(s) 1237
PleI GAGTC 2 cut(s) 291, 979
PpsI GAGTC 2 cut(s) 291, 979
Ppu21I YACGTR 1 cut(s) 1154
PscI ACATGT 2 cut(s) 626, 1198
Psp124BI GAGCTC 2 cut(s) 672, 693
Psp6I CCWGG 2 cut(s) 867, 1005
PspGI CCWGG 2 cut(s) 867, 1005
PspN4I GGNNCC 1 cut(s) 1070
PspPI GGNCC 4 cut(s) 301, 364, 490, 865
PstNI CAGNNNCTG 1 cut(s) 980
PsuI RGATCY 3 cut(s) 226, 580, 1058
RsaI GTAC 6 cut(s) 24, 29, 126, 135, 657, 1158
RsaNI GTAC 6 cut(s) 23, 28, 125, 134, 656, 1157
RseI CAYNNNNRTG 1 cut(s) 902
SacI GAGCTC 2 cut(s) 672, 693
SapI GCTCTTC 2 cut(s) 660, 1322
SaqAI TTAA 5 cut(s) 324, 387, 677, 822, 831
Sau3AI GATC 8 cut(s) 226, 402, 580, 716, 1039, 1046, 1058, 1261
Sau96I GGNCC 4 cut(s) 301, 364, 490, 865
SchI GAGTC 2 cut(s) 292, 980
ScrFI CCNGG 3 cut(s) 32, 869, 1007
SduI GDGCHC 4 cut(s) 672, 693, 1219, 1297
SfaNI GCATC 2 cut(s) 88, 110
SfcI CTRYAG 2 cut(s) 6, 1062
SfuI TTCGAA 1 cut(s) 1162
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 902
SmlI CTYRAG 3 cut(s) 731, 968, 1309
SmoI CTYRAG 3 cut(s) 731, 968, 1309
SspI AATATT 1 cut(s) 760
SspMI CTAG 2 cut(s) 378, 1194
SstI GAGCTC 2 cut(s) 672, 693
StyD4I CCNGG 3 cut(s) 30, 867, 1005
TaaI ACNGT 5 cut(s) 10, 27, 463, 791, 1116
TaiI ACGT 2 cut(s) 1156, 1344
TaqI TCGA 3 cut(s) 1074, 1162, 1251
TatI WGTACW 4 cut(s) 22, 124, 133, 1156
TfiI GAWTC 4 cut(s) 116, 167, 552, 707
Tru1I TTAA 5 cut(s) 324, 387, 677, 822, 831
Tru9I TTAA 5 cut(s) 324, 387, 677, 822, 831
TscAI CASTG 4 cut(s) 562, 794, 888, 981
TseFI GTSAC 1 cut(s) 785
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 7 cut(s) 48, 99, 170, 249, 286, 306, 1031
TspRI CASTG 4 cut(s) 562, 794, 888, 981
Van91I CCANNNNNTGG 1 cut(s) 1237
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 2 cut(s) 293, 768
XceI RCATGY 3 cut(s) 630, 746, 1202
XmiI GTMKAC 2 cut(s) 638, 747
XmnI GAANNNNTTC 3 cut(s) 423, 445, 1031
XspI CTAG 2 cut(s) 378, 1194
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.