Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0006680

Physical Location & Seq

Genomic Coordinates Forward (+)

8777893 .. 8780843

Gene Structure 2951 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1109000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGGAAACGACGGCGTTTGAAACAGTGGACCAAGTGGTGCAAGAGATCATGAGAATTCACAGATCTTTGCCGCCGAGGCCGGCCGTCGACGAGGTGGAAGTGGCGGCGGCGCTGGTTCGGAATGTGGAAATGGCGGAACAGGCCAGGCTCGACGCCATTGCCGGGCAGACCAAGAGCTTGCAAGTTCCGGAAGAGCTGTTCATGGTGCTGCAAGAGATGCAGAGGAACTTGGTCTACTTCAACAGCAAGGAGGAGATGCGGGAGGCCCGTAAGTTGCTCGATCTCGAGAACGTCCATAATCTGTTCGACGATTTGATTCAGAGAGCTTCCACTTGCGTCTCCTCTCCCGATTCAACTTCTTCCTCTTCTGCGGCGGCGAATTTGAGCTCGGGTTCGAATTCCGTTGCTGGGGTCCTTCCTCACCGGCCCTCTCCTTCGTATTCTTCGGGTTCTGCTGCTGCTGCTGCGTATGTGTTCAACACTGACAGAGAAGGTGTTGCTAAAAGTTCTTCAGCCAGAGTCAGCAGAGACGACAGTTTCGTGGTCAGCAAGGCCAAGAAAGCTTTCCACTTGGATGGAATTGGGGCTGGACCTGCTGTTTCTGCAACTCCCCGGATAGTGGATGCGTCTCTCAGGCCGGTTTCCGCTCTCAATTCAACCGGAGGAGATGGAGTTGGAGGTCAAGAGGGTGAGAAATTGAGTCTGATAAAGTTAGCGAGCTTAATTGAAGTGTTTGCAAAGAAAGGCACCAAGGATGTTAATCTTCGAAACAAGTTGATGGAACAAATTGAATGGTTGCCTGACTCGATAGGAAAGCTTTCGAGATTGGTCTCCCTTGATTTGTCCGAAAACCGGATTCTGGTCCTCCCAGCCACCATTGGAGGTCTTTCCTCCTTGGCCAAATTGGATTTGCACTCAAACAGGATTGCTCAGCTGCCTGACACTGTCGGAGATCTTCTTAGCCTTGTCTCTCTAGACCTTAGAGCAAACAACTTGACTACTCTGCCGGCTACCTTTGGCCGATTGGTGCATCTTGAAGAGCTCGATTTGAGCTCAAACAGGCTCACTGTGCTTCCTGACACAATTGGGTCCCTGGCTAGCCTCAAGACATTGAATGTTGAGACCAATGATATTGAAGAAATTCCTCATACTATTGGTCATTGTACCTCGCTCAAAGAGCTGCGTGCAGATTATAACCGTCTTAAAGCACTCCCTGAAGCTGTAGGAAAGATTGAGAGCCTGGAGGTTTTGTCTGTGCGTTACAATAACATCAAACAGTTGCCCACAACCGTGTCGTCTCTGTTGAGCCTGAGGGAGCTGGATGTGAGTTTCAATGAGCTTGAGTCAGTGCCTGAGAACTTGTGCTTTGCCACCTCACTTGTCAAGATGAACATTGGGAACAATTTCGCTGATCTGCGATCCCTGCCAAGGTCTATTGGGAACCTCGAGATGCTCGAAGAGTTGGATATCAGCAACAACCAGATACGAGTTCTCCCAGACTCTTTCAGGATGCTCACTCGACTACGTGTTCTACGTGTTGAAGAAAACCCTCTTGAAGTACCACCAAGACACATAGCTGAAAAGGGTGCACAAGCTGTTGTTCAATACGTGGCTGAGCTTGTCGCGAAAAGAGATGTTAAAGCACAACCTGTTAAGCAGAAAAAGACCTGGGCTCAAATCTGCTGCTTCTCGAGGTCAAACAAAAGGAAGCGCAATGGTGAAGAATATGTAAAAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8

Protein Analysis

580

Amino Acids

63.37

Weight (kDa)

6.1

Isoelectric Point (pI)

44.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	262 - 326	2.2e-08	Leucine-rich repeat region
LRR_8	PF13855	268 - 309	2.5e-06	Leucine rich repeat
LRR_8	PF13855	298 - 355	6.2e-11	Leucine rich repeat
LRR_8	PF13855	321 - 378	6.7e-11	Leucine rich repeat
LRR_14	PF23598	339 - 463	4.9e-18	Leucine-rich repeat region
LRR_8	PF13855	344 - 401	1.3e-09	Leucine rich repeat
LRR_4	PF12799	344 - 382	2.7e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	390 - 447	1.4e-06	Leucine rich repeat
LRR_8	PF13855	459 - 518	2.3e-06	Leucine rich repeat
LRR_14	PF23598	472 - 516	6.6e-08	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17440 AT4G35470 AT4G35470
fragaria_vesca	FvH4_1g10070 FvH4_1g10070 FvH4_1g10070
malus_domestica	MD02G1109000.v1.1 MD15G1228200.v1.1
prunus_persica	Prupe.7G186300_v2.0.a1
pyrus_communis	pycom02g08760 pycom02g08770 pycom15g20310
rosa_chinensis	RchiOBHm_Chr2g0096861
rosa_laevigata	RLG00000016674
rosa_multiflora	Rmu_co8410777.1_g000001 Rmu_ssc0000215.1_g000025
rosa_roxburghii	Rroxscaffold_2G00145260 Rroxscaffold_4G00329410 Rroxscaffold_4G00329650
rosa_rugosa	Rorug02G0061500 Rorug02G0061500
rosa_samantha	Rh2AG108900 Rh2BG111000 Rh2CG113100 Rh2DG112200
rosa_wichuraiana	Rw2G008430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1194
Acc36I	ACCTGC	1 cut(s)	601
AccB1I	GGYRCC	1 cut(s)	746
AccBSI	CCGCTC	1 cut(s)	647
AccI	GTMKAC	2 cut(s)	87, 234
AccII	CGCG	1 cut(s)	1625
AccIII	TCCGGA	1 cut(s)	187
AciI	CCGC	8 cut(s)	71, 104, 107, 134, 259, 371, 374, 645
AclWI	GGATC	1 cut(s)	1413
AcoI	YGGCCR	3 cut(s)	81, 897, 1018
AcsI	RAATTY	4 cut(s)	54, 379, 397, 1140
AcuI	CTGAAG	2 cut(s)	495, 1236
AcyI	GRCGYC	1 cut(s)	153
AfaI	GTAC	2 cut(s)	1165, 1560
AfiI	CCNNNNNNNGG	6 cut(s)	162, 408, 619, 852, 859, 1428
AflIII	ACRYGT	2 cut(s)	1525, 1534
AjnI	CCWGG	4 cut(s)	143, 1092, 1239, 1667
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AleI	CACNNNNGTG	1 cut(s)	1289
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
Alw21I	GWGCWC	4 cut(s)	389, 1044, 1055, 1591
Alw26I	GTCTC	7 cut(s)	343, 522, 633, 835, 973, 1115, 1302
Alw44I	GTGCAC	1 cut(s)	1587
AlwI	GGATC	1 cut(s)	1413
AlwNI	CAGNNNCTG	1 cut(s)	1352
Ama87I	CYCGRG	4 cut(s)	284, 388, 1445, 1690
Aor13HI	TCCGGA	1 cut(s)	187
AoxI	GGCC	9 cut(s)	77, 81, 141, 264, 425, 552, 635, 897, 1018
ApaLI	GTGCAC	1 cut(s)	1587
ApeKI	GCWGC	8 cut(s)	208, 455, 458, 461, 464, 934, 1180, 1683
ApoI	RAATTY	4 cut(s)	54, 379, 397, 1140
ArsI	GACNNNNNNTTYG	2 cut(s)	521, 553
Asp700I	GAANNNNTTC	4 cut(s)	563, 817, 1140, 1403
AspLEI	GCGC	2 cut(s)	112, 1713
AspS9I	GGNCC	7 cut(s)	28, 265, 412, 426, 590, 862, 1089
AsuC2I	CCSGG	2 cut(s)	163, 613
AsuHPI	GGTGA	3 cut(s)	413, 701, 1730
AsuII	TTCGAA	2 cut(s)	395, 766
AsuNHI	GCTAGC	1 cut(s)	1097
AvaI	CYCGRG	4 cut(s)	284, 388, 1445, 1690
AvaII	GGWCC	5 cut(s)	28, 412, 590, 862, 1089
AxyI	CCTNAGG	1 cut(s)	1310
BaeGI	GKGCMC	1 cut(s)	1591
BalI	TGGCCA	1 cut(s)	899
BanI	GGYRCC	1 cut(s)	746
BanII	GRGCYC	4 cut(s)	389, 1044, 1055, 1675
Bbv12I	GWGCWC	4 cut(s)	389, 1044, 1055, 1591
BbvI	GCAGC	8 cut(s)	195, 442, 445, 448, 451, 921, 1167, 1670
BccI	CCATC	3 cut(s)	569, 662, 772
BceAI	ACGGC	2 cut(s)	27, 68
BcgI	CGANNNNNNTGC	2 cut(s)	140, 174
BciT130I	CCWGG	4 cut(s)	145, 1094, 1241, 1669
BcnI	CCSGG	2 cut(s)	163, 613
BcoDI	GTCTC	7 cut(s)	343, 522, 633, 835, 973, 1115, 1302
BfaI	CTAG	2 cut(s)	974, 1098
BfmI	CTRYAG	1 cut(s)	1221
BfoI	RGCGCY	1 cut(s)	113
BfuAI	ACCTGC	1 cut(s)	601
BglI	GCCNNNNNGGC	1 cut(s)	76
BglII	AGATCT	2 cut(s)	62, 952
BlpI	GCTNAGC	2 cut(s)	930, 1614
Bme1390I	CCNGG	6 cut(s)	145, 163, 613, 1094, 1241, 1669
Bme18I	GGWCC	5 cut(s)	28, 412, 590, 862, 1089
BmeT110I	CYCGRG	4 cut(s)	284, 388, 1445, 1690
BmgT120I	GGNCC	7 cut(s)	28, 265, 412, 426, 590, 862, 1089
BmiI	GGNNCC	5 cut(s)	413, 748, 1090, 1091, 1442
BmrFI	CCNGG	6 cut(s)	145, 163, 613, 1094, 1241, 1669
BmsI	GCATC	6 cut(s)	207, 246, 613, 1039, 1440, 1500
BmtI	GCTAGC	1 cut(s)	1101
BpmI	CTGGAG	1 cut(s)	1262
Bpu1102I	GCTNAGC	2 cut(s)	930, 1614
Bpu14I	TTCGAA	2 cut(s)	395, 766
BpuEI	CTTGAG	2 cut(s)	1088, 1361
BpuMI	CCSGG	2 cut(s)	163, 613
BsaAI	YACGTR	3 cut(s)	1526, 1535, 1609
BsaBI	GATNNNNATC	1 cut(s)	759
BsaHI	GRCGYC	1 cut(s)	153
BsaI	GGTCTC	2 cut(s)	835, 1115
BsaJI	CCNNGG	7 cut(s)	74, 611, 750, 894, 1092, 1427, 1668
BsaWI	WCCGGW	3 cut(s)	187, 659, 852
BsaXI	ACNNNNNCTCC	6 cut(s)	657, 687, 1235, 1265, 1476, 1506
Bsc4I	CCNNNNNNNGG	6 cut(s)	162, 408, 619, 852, 859, 1428
Bse118I	RCCGGY	4 cut(s)	79, 423, 637, 1006
Bse21I	CCTNAGG	1 cut(s)	1310
Bse3DI	GCAATG	2 cut(s)	156, 1720
Bse8I	GATNNNNATC	1 cut(s)	759
BseAI	TCCGGA	1 cut(s)	187
BseBI	CCWGG	4 cut(s)	145, 1094, 1241, 1669
BseDI	CCNNGG	7 cut(s)	74, 611, 750, 894, 1092, 1427, 1668
BseGI	GGATG	5 cut(s)	580, 628, 760, 1327, 1515
BseJI	GATNNNNATC	1 cut(s)	759
BseLI	CCNNNNNNNGG	6 cut(s)	162, 408, 619, 852, 859, 1428
BseMI	GCAATG	2 cut(s)	156, 1720
BseMII	CTCAG	5 cut(s)	646, 944, 1301, 1344, 1605
BseRI	GAGGAG	3 cut(s)	266, 331, 678
BseSI	GKGCMC	1 cut(s)	1591
BseX3I	CGGCCG	1 cut(s)	81
BseXI	GCAGC	8 cut(s)	195, 442, 445, 448, 451, 921, 1167, 1670
BseYI	CCCAGC	2 cut(s)	407, 868
BsgI	GTGCAG	1 cut(s)	1206
Bsh1236I	CGCG	1 cut(s)	1625
Bsh1285I	CGRYCG	1 cut(s)	84
BshFI	GGCC	9 cut(s)	79, 83, 143, 266, 427, 554, 637, 899, 1020
BshNI	GGYRCC	1 cut(s)	746
BsiEI	CGRYCG	1 cut(s)	84
BsiHKAI	GWGCWC	4 cut(s)	389, 1044, 1055, 1591
BsiHKCI	CYCGRG	4 cut(s)	284, 388, 1445, 1690
BsiSI	CCGG	9 cut(s)	80, 162, 188, 424, 613, 638, 660, 853, 1007
BslFI	GGGAC	1 cut(s)	1075
BslI	CCNNNNNNNGG	6 cut(s)	162, 408, 619, 852, 859, 1428
BsmAI	GTCTC	7 cut(s)	343, 522, 633, 835, 973, 1115, 1302
BsmBI	CGTCTC	4 cut(s)	343, 522, 633, 1302
BsmFI	GGGAC	1 cut(s)	1075
BsnI	GGCC	9 cut(s)	79, 83, 143, 266, 427, 554, 637, 899, 1020
Bso31I	GGTCTC	2 cut(s)	835, 1115
BsoBI	CYCGRG	4 cut(s)	284, 388, 1445, 1690
Bsp119I	TTCGAA	2 cut(s)	395, 766
Bsp1286I	GDGCHC	5 cut(s)	389, 1044, 1055, 1591, 1675
Bsp13I	TCCGGA	1 cut(s)	187
Bsp143I	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
Bsp1720I	GCTNAGC	2 cut(s)	930, 1614
Bsp68I	TCGCGA	1 cut(s)	1625
BspACI	CCGC	8 cut(s)	71, 104, 107, 134, 259, 371, 374, 645
BspANI	GGCC	9 cut(s)	79, 83, 143, 266, 427, 554, 637, 899, 1020
BspCNI	CTCAG	5 cut(s)	645, 943, 1302, 1345, 1606
BspEI	TCCGGA	1 cut(s)	187
BspFNI	CGCG	1 cut(s)	1625
BspHI	TCATGA	1 cut(s)	48
BspLI	GGNNCC	5 cut(s)	413, 748, 1090, 1091, 1442
BspMI	ACCTGC	1 cut(s)	601
BspOI	GCTAGC	1 cut(s)	1101
BspPI	GGATC	1 cut(s)	1413
BspQI	GCTCTTC	2 cut(s)	186, 1032
BspT104I	TTCGAA	2 cut(s)	395, 766
BspT107I	GGYRCC	1 cut(s)	746
BspTNI	GGTCTC	2 cut(s)	835, 1115
BsrBI	CCGCTC	1 cut(s)	647
BsrDI	GCAATG	2 cut(s)	156, 1720
BsrFI	RCCGGY	4 cut(s)	79, 423, 637, 1006
BssAI	RCCGGY	4 cut(s)	79, 423, 637, 1006
BssECI	CCNNGG	7 cut(s)	74, 611, 750, 894, 1092, 1427, 1668
BssMI	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
BssNI	GRCGYC	1 cut(s)	153
BssT1I	CCWWGG	3 cut(s)	750, 894, 1427
Bst2UI	CCWGG	4 cut(s)	145, 1094, 1241, 1669
Bst4CI	ACNGT	7 cut(s)	25, 536, 946, 1069, 1199, 1278, 1291
Bst6I	CTCTTC	4 cut(s)	186, 370, 1032, 1452
BstACI	GRCGYC	1 cut(s)	153
BstAPI	GCANNNNNTGC	1 cut(s)	217
BstBAI	YACGTR	3 cut(s)	1526, 1535, 1609
BstBI	TTCGAA	2 cut(s)	395, 766
BstC8I	GCNNGC	6 cut(s)	81, 179, 718, 1008, 1099, 1185
BstDEI	CTNAG	7 cut(s)	632, 930, 959, 980, 1310, 1353, 1614
BstF5I	GGATG	5 cut(s)	580, 628, 760, 1327, 1515
BstFNI	CGCG	1 cut(s)	1625
BstH2I	RGCGCY	1 cut(s)	113
BstHHI	GCGC	2 cut(s)	112, 1713
BstKTI	GATC	6 cut(s)	48, 65, 283, 955, 1414, 1421
BstMAI	GTCTC	7 cut(s)	343, 522, 633, 835, 973, 1115, 1302
BstMBI	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
BstMCI	CGRYCG	1 cut(s)	84
BstNI	CCWGG	4 cut(s)	145, 1094, 1241, 1669
BstSCI	CCNGG	6 cut(s)	143, 161, 611, 1092, 1239, 1667
BstSFI	CTRYAG	1 cut(s)	1221
BstSLI	GKGCMC	1 cut(s)	1591
BstUI	CGCG	1 cut(s)	1625
BstV1I	GCAGC	8 cut(s)	195, 442, 445, 448, 451, 921, 1167, 1670
BstX2I	RGATCY	2 cut(s)	62, 952
BstXI	CCANNNNNNTGG	1 cut(s)	575
BstYI	RGATCY	2 cut(s)	62, 952
BstZI	CGGCCG	1 cut(s)	81
Bsu36I	CCTNAGG	1 cut(s)	1310
BsuRI	GGCC	9 cut(s)	79, 83, 143, 266, 427, 554, 637, 899, 1020
BtsCI	GGATG	5 cut(s)	580, 628, 760, 1327, 1515
BtsIMutI	CAGTG	5 cut(s)	30, 480, 942, 1065, 1353
BtuMI	TCGCGA	1 cut(s)	1625
BveI	ACCTGC	1 cut(s)	601
Cac8I	GCNNGC	6 cut(s)	81, 179, 718, 1008, 1099, 1185
CaiI	CAGNNNCTG	1 cut(s)	1352
CciI	TCATGA	1 cut(s)	48
CfoI	GCGC	2 cut(s)	112, 1713
Cfr10I	RCCGGY	4 cut(s)	79, 423, 637, 1006
Cfr13I	GGNCC	7 cut(s)	28, 265, 412, 426, 590, 862, 1089
CseI	GACGC	3 cut(s)	161, 325, 615
Csp6I	GTAC	2 cut(s)	1164, 1559
CspCI	CAANNNNNGTGG	2 cut(s)	1360, 1395
CviAII	CATG	2 cut(s)	49, 202
CviQI	GTAC	2 cut(s)	1164, 1559
DdeI	CTNAG	7 cut(s)	632, 930, 959, 980, 1310, 1353, 1614
DpnI	GATC	6 cut(s)	47, 64, 282, 954, 1413, 1420
DpnII	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
EaeI	YGGCCR	3 cut(s)	81, 897, 1018
EagI	CGGCCG	1 cut(s)	81
Eam1104I	CTCTTC	4 cut(s)	186, 370, 1032, 1452
EarI	CTCTTC	4 cut(s)	186, 370, 1032, 1452
EciI	GGCGGA	1 cut(s)	149
Ecl136II	GAGCTC	3 cut(s)	387, 1042, 1053
EclXI	CGGCCG	1 cut(s)	81
Eco130I	CCWWGG	3 cut(s)	750, 894, 1427
Eco24I	GRGCYC	4 cut(s)	389, 1044, 1055, 1675
Eco31I	GGTCTC	2 cut(s)	835, 1115
Eco32I	GATATC	1 cut(s)	1468
Eco47I	GGWCC	5 cut(s)	28, 412, 590, 862, 1089
Eco52I	CGGCCG	1 cut(s)	81
Eco53kI	GAGCTC	3 cut(s)	387, 1042, 1053
Eco57I	CTGAAG	2 cut(s)	495, 1236
Eco81I	CCTNAGG	1 cut(s)	1310
Eco88I	CYCGRG	4 cut(s)	284, 388, 1445, 1690
EcoICRI	GAGCTC	3 cut(s)	387, 1042, 1053
EcoO109I	RGGNCCY	2 cut(s)	412, 1089
EcoRI	GAATTC	2 cut(s)	54, 397
EcoRII	CCWGG	4 cut(s)	143, 1092, 1239, 1667
EcoRV	GATATC	1 cut(s)	1468
EcoT14I	CCWWGG	3 cut(s)	750, 894, 1427
EcoT38I	GRGCYC	4 cut(s)	389, 1044, 1055, 1675
ErhI	CCWWGG	3 cut(s)	750, 894, 1427
Esp3I	CGTCTC	4 cut(s)	343, 522, 633, 1302
FaeI	CATG	2 cut(s)	52, 205
FaiI	YATR	8 cut(s)	50, 203, 297, 471, 1149, 1194, 1574, 1728
FaqI	GGGAC	1 cut(s)	1075
FatI	CATG	2 cut(s)	48, 201
FauI	CCCGC	1 cut(s)	252
FblI	GTMKAC	2 cut(s)	87, 234
FokI	GGATG	5 cut(s)	587, 635, 767, 1334, 1522
FriOI	GRGCYC	4 cut(s)	389, 1044, 1055, 1675
FseI	GGCCGGCC	1 cut(s)	83
FspBI	CTAG	2 cut(s)	974, 1098
GlaI	GCGC	2 cut(s)	111, 1712
GsaI	CCCAGC	2 cut(s)	411, 872
GsuI	CTGGAG	1 cut(s)	1262
HaeII	RGCGCY	1 cut(s)	113
HaeIII	GGCC	9 cut(s)	79, 83, 143, 266, 427, 554, 637, 899, 1020
HapII	CCGG	9 cut(s)	80, 162, 188, 424, 613, 638, 660, 853, 1007
HgaI	GACGC	3 cut(s)	161, 325, 615
HhaI	GCGC	2 cut(s)	112, 1713
Hin1I	GRCGYC	1 cut(s)	153
Hin1II	CATG	2 cut(s)	52, 205
Hin6I	GCGC	2 cut(s)	110, 1711
HinP1I	GCGC	2 cut(s)	110, 1711
HincII	GTYRAC	1 cut(s)	88
HindII	GTYRAC	1 cut(s)	88
HindIII	AAGCTT	2 cut(s)	561, 815
HinfI	GANTC	8 cut(s)	316, 350, 519, 700, 803, 856, 1343, 1499
HpaII	CCGG	9 cut(s)	80, 162, 188, 424, 613, 638, 660, 853, 1007
HphI	GGTGA	3 cut(s)	413, 701, 1730
Hpy166II	GTNNAC	4 cut(s)	28, 88, 235, 1589
Hpy188I	TCNGA	5 cut(s)	120, 321, 705, 847, 950
Hpy8I	GTNNAC	4 cut(s)	28, 88, 235, 1589
Hpy99I	CGWCG	5 cut(s)	13, 89, 92, 155, 311
HpyAV	CCTTC	3 cut(s)	425, 444, 485
HpyCH4III	ACNGT	7 cut(s)	25, 536, 946, 1069, 1199, 1278, 1291
HpyCH4IV	ACGT	4 cut(s)	291, 1525, 1534, 1608
HpyF3I	CTNAG	7 cut(s)	632, 930, 959, 980, 1310, 1353, 1614
HpySE526I	ACGT	4 cut(s)	291, 1525, 1534, 1608
Hsp92I	GRCGYC	1 cut(s)	153
Hsp92II	CATG	2 cut(s)	52, 205
HspAI	GCGC	2 cut(s)	110, 1711
KflI	GGGWCCC	1 cut(s)	1089
Kpn2I	TCCGGA	1 cut(s)	187
KroI	GCCGGC	2 cut(s)	79, 1006
KroNI	GCCGGC	2 cut(s)	81, 1008
Kzo9I	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
LguI	GCTCTTC	2 cut(s)	186, 1032
LmnI	GCTCC	1 cut(s)	1315
Lsp1109I	GCAGC	8 cut(s)	195, 442, 445, 448, 451, 921, 1167, 1670
LweI	GCATC	6 cut(s)	207, 246, 613, 1039, 1440, 1500
MaeI	CTAG	2 cut(s)	974, 1098
MaeII	ACGT	4 cut(s)	291, 1525, 1534, 1608
MaeIII	GTNAC	1 cut(s)	1259
MalI	GATC	6 cut(s)	47, 64, 282, 954, 1413, 1420
MbiI	CCGCTC	1 cut(s)	647
MboI	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
MfeI	CAATTG	1 cut(s)	1083
MflI	RGATCY	2 cut(s)	62, 952
MhlI	GDGCHC	5 cut(s)	389, 1044, 1055, 1591, 1675
MlsI	TGGCCA	1 cut(s)	899
MluNI	TGGCCA	1 cut(s)	899
MlyI	GAGTC	5 cut(s)	528, 709, 797, 1352, 1493
MmeI	TCCRAC	3 cut(s)	655, 928, 1443
Mox20I	TGGCCA	1 cut(s)	899
MroI	TCCGGA	1 cut(s)	187
MroNI	GCCGGC	2 cut(s)	79, 1006
MroXI	GAANNNNTTC	4 cut(s)	563, 817, 1140, 1403
MscI	TGGCCA	1 cut(s)	899
MseI	TTAA	5 cut(s)	722, 759, 1203, 1638, 1653
MslI	CAYNNNNRTG	2 cut(s)	573, 1289
Msp20I	TGGCCA	1 cut(s)	899
MspA1I	CMGCKG	1 cut(s)	934
MspI	CCGG	9 cut(s)	80, 162, 188, 424, 613, 638, 660, 853, 1007
MspR9I	CCNGG	6 cut(s)	145, 163, 613, 1094, 1241, 1669
MunI	CAATTG	1 cut(s)	1083
MvaI	CCWGG	4 cut(s)	145, 1094, 1241, 1669
MvnI	CGCG	1 cut(s)	1625
NaeI	GCCGGC	2 cut(s)	81, 1008
NciI	CCSGG	2 cut(s)	163, 613
NdeII	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
NgoMIV	GCCGGC	2 cut(s)	79, 1006
NheI	GCTAGC	1 cut(s)	1097
NlaIII	CATG	2 cut(s)	52, 205
NlaIV	GGNNCC	5 cut(s)	413, 748, 1090, 1091, 1442
NmeAIII	GCCGAG	1 cut(s)	99
NruI	TCGCGA	1 cut(s)	1625
NspV	TTCGAA	2 cut(s)	395, 766
OliI	CACNNNNGTG	1 cut(s)	1289
PaeR7I	CTCGAG	3 cut(s)	284, 1445, 1690
PagI	TCATGA	1 cut(s)	48
PciSI	GCTCTTC	2 cut(s)	186, 1032
PcsI	WCGNNNNNNNCGW	3 cut(s)	537, 1452, 1531
PdiI	GCCGGC	2 cut(s)	81, 1008
PdmI	GAANNNNTTC	4 cut(s)	563, 817, 1140, 1403
PfeI	GAWTC	3 cut(s)	316, 350, 856
PflFI	GACNNNGTC	1 cut(s)	944
PleI	GAGTC	5 cut(s)	527, 708, 797, 1351, 1493
PpsI	GAGTC	5 cut(s)	527, 708, 797, 1351, 1493
Ppu21I	YACGTR	3 cut(s)	1526, 1535, 1609
PpuMI	RGGWCCY	2 cut(s)	412, 1089
PsiI	TTATAA	1 cut(s)	1194
Psp124BI	GAGCTC	3 cut(s)	389, 1044, 1055
Psp5II	RGGWCCY	2 cut(s)	412, 1089
Psp6I	CCWGG	4 cut(s)	143, 1092, 1239, 1667
PspFI	CCCAGC	2 cut(s)	407, 868
PspGI	CCWGG	4 cut(s)	143, 1092, 1239, 1667
PspN4I	GGNNCC	5 cut(s)	413, 748, 1090, 1091, 1442
PspPI	GGNCC	7 cut(s)	28, 265, 412, 426, 590, 862, 1089
PspPPI	RGGWCCY	2 cut(s)	412, 1089
PsrI	GAACNNNNNNTAC	2 cut(s)	218, 250
PstNI	CAGNNNCTG	1 cut(s)	1352
PsuI	RGATCY	2 cut(s)	62, 952
PsyI	GACNNNGTC	1 cut(s)	944
PvuII	CAGCTG	1 cut(s)	934
RigI	GGCCGGCC	1 cut(s)	83
RruI	TCGCGA	1 cut(s)	1625
RsaI	GTAC	2 cut(s)	1165, 1560
RsaNI	GTAC	2 cut(s)	1164, 1559
RseI	CAYNNNNRTG	2 cut(s)	573, 1289
SacI	GAGCTC	3 cut(s)	389, 1044, 1055
SalI	GTCGAC	1 cut(s)	86
SapI	GCTCTTC	2 cut(s)	186, 1032
SaqAI	TTAA	5 cut(s)	722, 759, 1203, 1638, 1653
Sau3AI	GATC	6 cut(s)	45, 62, 280, 952, 1411, 1418
Sau96I	GGNCC	7 cut(s)	28, 265, 412, 426, 590, 862, 1089
SchI	GAGTC	5 cut(s)	528, 709, 797, 1352, 1493
ScrFI	CCNGG	6 cut(s)	145, 163, 613, 1094, 1241, 1669
SduI	GDGCHC	5 cut(s)	389, 1044, 1055, 1591, 1675
SfaNI	GCATC	6 cut(s)	207, 246, 613, 1039, 1440, 1500
SfcI	CTRYAG	1 cut(s)	1221
Sfr274I	CTCGAG	3 cut(s)	284, 1445, 1690
SfuI	TTCGAA	2 cut(s)	395, 766
SgrDI	CGTCGACG	1 cut(s)	86
SinI	GGWCC	5 cut(s)	28, 412, 590, 862, 1089
SlaI	CTCGAG	3 cut(s)	284, 1445, 1690
SmiMI	CAYNNNNRTG	2 cut(s)	573, 1289
SmlI	CTYRAG	5 cut(s)	284, 1103, 1340, 1445, 1690
SmoI	CTYRAG	5 cut(s)	284, 1103, 1340, 1445, 1690
SsiI	CCGC	8 cut(s)	71, 104, 107, 134, 259, 371, 374, 645
SspMI	CTAG	2 cut(s)	974, 1098
SstI	GAGCTC	3 cut(s)	389, 1044, 1055
StyD4I	CCNGG	6 cut(s)	143, 161, 611, 1092, 1239, 1667
StyI	CCWWGG	3 cut(s)	750, 894, 1427
TaaI	ACNGT	7 cut(s)	25, 536, 946, 1069, 1199, 1278, 1291
TaiI	ACGT	4 cut(s)	294, 1528, 1537, 1611
TauI	GCSGC	5 cut(s)	73, 107, 110, 374, 377
TfiI	GAWTC	3 cut(s)	316, 350, 856
Tru1I	TTAA	5 cut(s)	722, 759, 1203, 1638, 1653
Tru9I	TTAA	5 cut(s)	722, 759, 1203, 1638, 1653
TscAI	CASTG	5 cut(s)	30, 487, 949, 1072, 1353
TseI	GCWGC	8 cut(s)	208, 455, 458, 461, 464, 934, 1180, 1683
TspDTI	ATGAA	2 cut(s)	190, 1403
TspGWI	ACGGA	1 cut(s)	391
TspRI	CASTG	5 cut(s)	30, 487, 949, 1072, 1353
Tth111I	GACNNNGTC	1 cut(s)	944
VneI	GTGCAC	1 cut(s)	1587
VpaK11BI	GGWCC	5 cut(s)	28, 412, 590, 862, 1089
XapI	RAATTY	4 cut(s)	54, 379, 397, 1140
XbaI	TCTAGA	1 cut(s)	973
XhoI	CTCGAG	3 cut(s)	284, 1445, 1690
XmiI	GTMKAC	2 cut(s)	87, 234
XmnI	GAANNNNTTC	4 cut(s)	563, 817, 1140, 1403
XspI	CTAG	2 cut(s)	974, 1098

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.