Rw2G008430

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006680
Physical Location & Seq
Forward (+)
8347165 .. 8350810
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008430.1

Sequence Viewer

Length: 1686 bp
ATGGAGTCGACGGCGGAGTCGGTGGACCAAGTGGTGGAGGAGATTATGAGAATCCACAGATCCTTGCCGCCGCGGCCCGGCATCGACGAGGTGGAGGCGGCGAAGGCGCTGATTCAAAATGTGGAGAAGGAGGACCAGGCCAGGCTGGAAGCCATTGCTAAACAGACCAAGAGCAACAACGTCTCCGAAGAGCTCTTCATGGTTTTGCAGGAGATGCAGAAGAACATGGTCTACTTCAACAGCAGAGAGCAGAAGCAGGAGGCCATGAAGTTGCTCGATCTTGAAAATCTTCACGAATTGTTCGACGATTATATCCAGAGAGCTTCCACCTGTGTTTCTTCTTCTTCTTCGGCTCCGTCGTCTTACACGAAGTCGATTCCCAAGGCTAGACCTAAGCCGTTGGATTCCGCCGCGGCGTACGTGTACCGCTCCGACAGAGAAGTCGGTAAGGCTAAAGCCATGGTCAGCCGAGACGACAGTTACGTGGTCAACAAGGCCAAGAAGAGCTTCTACGCCGACGGAATTGGGCCTGGGCCGGCGGTTTCCGCCACGCCGCAGATTGCCGATTCGTCTCTCAAGTCTGGTCTCGCTCTTGCTTCATCTGGCGCTGGAGGTCAAGAGGGTGAGAAATTGAGTTTGATAAAGCTAGCGAGTCTAATTGAAGTCTTGGCCAAGAAAGGGACTAAGGAGGTTAATCTTCGAAACAAGTTGAATGACCAAATCGAGTGGCTACCTGATTCGATAGGAAAGCTTTCGAGTTTGCTGACTCTTGATTTGTCTGAAAATCGGCTTTTGGTCCTGCCATCCACCATTGGAGGACTTTCGACCTTGACCAAATTGGATTTGCATTCCAACAGGCTCACTGAGCTACCGGACACTTTTGGGGATCTTCTTAGCCTTGTCCATCTTGACCTTAGGGCAAACAATTTGGCATCTCTGCCTGCTACATTTGGCCGATTGGTGCGTCTTGAAGAGCTGGACTTGAGCTCAAATAGGCTCTCTTTGCTTCCTGACTCAATTGGGTCGCTTGCTAGTCTCAAGAGCTTGAATGTGGAGACCAATGAGATTGATGAAATTCCTCATAGCATTGGTCATTGCACGAACCTTAGAGAGCTCCATGCTGATTATAACCGGCTTAAAGCACTTCCAGAAGCCGTAGGAAAGATTGAGACCCTGGAGATTTTGTCAGTGCGTTACAACAACATCAAGCAGTTGCCCACAACCGTGTCATCTCTACTGAGCTTGAGGGAACTGGATGTGAGTTTCAATGAGCTTGAGTCAGTGCCTGAGAACTTATGTTTTGCCACCTCGCTGGTCAAGATGAACGTAGGAAACAATTTTGCTGATCTGCGGTCACTACCAAGGTCCATTGGGAACCTCGAGATGCTTGAAGAGTTGGATATCAGCAATAACCAGATACGGTTTCTCCCCGACTCTTTCAGGATGCTCACACGACTACGTGTTCTACGTGTAGAAGAAAACCCTCTTGAAGTGCCTCCTAGACATATAGCTGACAAGGGAGCGCAAGCTGTTGTCCAATACATGGCAGAGCTTGTCGAGAAAAGAGAAGTTAAAGTACAACCGACTAAGCAAAAAAAGAGTTGGCTCTACAATGCAATATCCTGCACCCAACCTAAGCATGAGTTTAACGACCAAAGGGGTTCAAGCCTATTAAATTCGATTTAG

Protein Analysis

561

Amino Acids

62.24

Weight (kDa)

5.7

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 239 - 304 1.9e-09 Leucine-rich repeat region
LRR_8 PF13855 246 - 287 8.2e-06 Leucine rich repeat
LRR_8 PF13855 276 - 333 1.4e-11 Leucine rich repeat
LRR_14 PF23598 277 - 377 1e-12 Leucine-rich repeat region
LRR_14 PF23598 378 - 438 9.3e-06 Leucine-rich repeat region
LRR_8 PF13855 437 - 496 3.5e-06 Leucine rich repeat
LRR_14 PF23598 450 - 494 1.6e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1128
AasI GACNNNNNNGTC 2 cut(s) 16, 440
AccB7I CCANNNNNTGG 2 cut(s) 34, 1543
AccBSI CCGCTC 1 cut(s) 429
AccI GTMKAC 2 cut(s) 8, 231
AccII CGCG 2 cut(s) 73, 413
AclWI GGATC 2 cut(s) 54, 894
AcoI YGGCCR 2 cut(s) 669, 952
AcsI RAATTY 2 cut(s) 1074, 1675
AfaI GTAC 3 cut(s) 419, 425, 1578
AfiI CCNNNNNNNGG 4 cut(s) 34, 77, 678, 1543
AflIII ACRYGT 3 cut(s) 420, 1459, 1468
AjnI CCWGG 4 cut(s) 135, 140, 529, 1173
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AleI CACNNNNGTG 1 cut(s) 1223
Alw21I GWGCWC 3 cut(s) 195, 989, 1116
Alw26I GTCTC 7 cut(s) 187, 465, 576, 590, 1040, 1049, 1163
AlwI GGATC 2 cut(s) 54, 894
AlwNI CAGNNNCTG 1 cut(s) 1286
Ama87I CYCGRG 1 cut(s) 1379
AoxI GGCC 8 cut(s) 74, 138, 261, 495, 527, 533, 669, 952
ApoI RAATTY 2 cut(s) 1074, 1675
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
Asp700I GAANNNNTTC 3 cut(s) 288, 506, 751
AspLEI GCGC 3 cut(s) 109, 608, 1525
AspS9I GGNCC 7 cut(s) 25, 75, 133, 527, 533, 796, 1365
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 635
AsuII TTCGAA 1 cut(s) 700
AsuNHI GCTAGC 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 4 cut(s) 25, 133, 796, 1365
AxyI CCTNAGG 1 cut(s) 914
BalI TGGCCA 1 cut(s) 671
BanII GRGCYC 3 cut(s) 195, 989, 1116
BarI GAAGNNNNNNTAC 4 cut(s) 494, 526, 1560, 1592
Bbv12I GWGCWC 3 cut(s) 195, 989, 1116
BccI CCATC 2 cut(s) 811, 912
BceAI ACGGC 3 cut(s) 27, 382, 1139
BciT130I CCWGG 4 cut(s) 137, 142, 531, 1175
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 7 cut(s) 187, 465, 576, 590, 1040, 1049, 1163
BfaI CTAG 4 cut(s) 387, 647, 1032, 1500
BfoI RGCGCY 2 cut(s) 110, 609
BglI GCCNNNNNGGC 1 cut(s) 73
BisI GCNGC 7 cut(s) 68, 71, 74, 99, 411, 414, 554
BlsI GCNGC 7 cut(s) 69, 72, 75, 100, 412, 415, 555
Bme1390I CCNGG 5 cut(s) 78, 137, 142, 531, 1175
Bme18I GGWCC 4 cut(s) 25, 133, 796, 1365
BmeT110I CYCGRG 1 cut(s) 1379
BmgT120I GGNCC 7 cut(s) 25, 75, 133, 527, 533, 796, 1365
BmiI GGNNCC 2 cut(s) 354, 1376
BmrFI CCNGG 5 cut(s) 78, 137, 142, 531, 1175
BmsI GCATC 5 cut(s) 90, 204, 941, 1374, 1434
BmtI GCTAGC 1 cut(s) 650
BpmI CTGGAG 2 cut(s) 630, 1196
Bpu10I CCTNAGC 2 cut(s) 393, 1635
Bpu14I TTCGAA 1 cut(s) 700
BpuEI CTTGAG 5 cut(s) 560, 1003, 1022, 1264, 1295
BpuMI CCSGG 1 cut(s) 78
BsaAI YACGTR 4 cut(s) 421, 484, 1460, 1469
BsaI GGTCTC 3 cut(s) 590, 1049, 1163
BsaJI CCNNGG 7 cut(s) 71, 381, 411, 459, 530, 1173, 1361
BsaWI WCCGGW 1 cut(s) 871
BsaXI ACNNNNNCTCC 6 cut(s) 167, 197, 1169, 1199, 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 77, 678, 1543
Bse118I RCCGGY 2 cut(s) 535, 1131
Bse1I ACTGG 1 cut(s) 1257
Bse21I CCTNAGG 1 cut(s) 914
Bse3DI GCAATG 2 cut(s) 153, 1093
BseBI CCWGG 4 cut(s) 137, 142, 531, 1175
BseDI CCNNGG 7 cut(s) 71, 381, 411, 459, 530, 1173, 1361
BseGI GGATG 3 cut(s) 803, 1261, 1449
BseLI CCNNNNNNNGG 4 cut(s) 34, 77, 678, 1543
BseMI GCAATG 2 cut(s) 153, 1093
BseMII CTCAG 3 cut(s) 855, 1229, 1278
BseNI ACTGG 1 cut(s) 1257
BseRI GAGGAG 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 1609
Bsh1236I CGCG 2 cut(s) 73, 413
BshFI GGCC 8 cut(s) 76, 140, 263, 497, 529, 535, 671, 954
BsiHKAI GWGCWC 3 cut(s) 195, 989, 1116
BsiHKCI CYCGRG 1 cut(s) 1379
BsiSI CCGG 4 cut(s) 78, 536, 872, 1132
BsiWI CGTACG 1 cut(s) 417
BslFI GGGAC 1 cut(s) 694
BslI CCNNNNNNNGG 4 cut(s) 34, 77, 678, 1543
BsmAI GTCTC 7 cut(s) 187, 465, 576, 590, 1040, 1049, 1163
BsmBI CGTCTC 3 cut(s) 187, 465, 576
BsmFI GGGAC 1 cut(s) 694
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 8 cut(s) 76, 140, 263, 497, 529, 535, 671, 954
Bso31I GGTCTC 3 cut(s) 590, 1049, 1163
BsoBI CYCGRG 1 cut(s) 1379
Bsp119I TTCGAA 1 cut(s) 700
Bsp1286I GDGCHC 3 cut(s) 195, 989, 1116
Bsp143I GATC 4 cut(s) 59, 277, 886, 1345
Bsp19I CCATGG 1 cut(s) 459
BspANI GGCC 8 cut(s) 76, 140, 263, 497, 529, 535, 671, 954
BspCNI CTCAG 3 cut(s) 856, 1230, 1279
BspFNI CGCG 2 cut(s) 73, 413
BspLI GGNNCC 2 cut(s) 354, 1376
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 2 cut(s) 54, 894
BspQI GCTCTTC 4 cut(s) 183, 200, 497, 966
BspT104I TTCGAA 1 cut(s) 700
BspTNI GGTCTC 3 cut(s) 590, 1049, 1163
BsrBI CCGCTC 1 cut(s) 429
BsrDI GCAATG 2 cut(s) 153, 1093
BsrFI RCCGGY 2 cut(s) 535, 1131
BsrI ACTGG 1 cut(s) 1257
BssAI RCCGGY 2 cut(s) 535, 1131
BssECI CCNNGG 7 cut(s) 71, 381, 411, 459, 530, 1173, 1361
BssMI GATC 4 cut(s) 59, 277, 886, 1345
BssT1I CCWWGG 3 cut(s) 381, 459, 1361
Bst2UI CCWGG 4 cut(s) 137, 142, 531, 1175
Bst4CI ACNGT 3 cut(s) 479, 1225, 1422
Bst6I CTCTTC 5 cut(s) 183, 200, 497, 966, 1386
BstAPI GCANNNNNTGC 1 cut(s) 214
BstBAI YACGTR 4 cut(s) 421, 484, 1460, 1469
BstBI TTCGAA 1 cut(s) 700
BstC8I GCNNGC 5 cut(s) 537, 648, 942, 1029, 1527
BstDSI CCRYGG 3 cut(s) 71, 411, 459
BstF5I GGATG 3 cut(s) 803, 1261, 1449
BstFNI CGCG 2 cut(s) 73, 413
BstH2I RGCGCY 2 cut(s) 110, 609
BstHHI GCGC 3 cut(s) 109, 608, 1525
BstKTI GATC 4 cut(s) 62, 280, 889, 1348
BstMAI GTCTC 7 cut(s) 187, 465, 576, 590, 1040, 1049, 1163
BstMBI GATC 4 cut(s) 59, 277, 886, 1345
BstMWI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 545, 865, 1003
BstNI CCWGG 4 cut(s) 137, 142, 531, 1175
BstSCI CCNGG 5 cut(s) 76, 135, 140, 529, 1173
BstUI CGCG 2 cut(s) 73, 413
BstX2I RGATCY 2 cut(s) 59, 886
BstXI CCANNNNNNTGG 1 cut(s) 1312
BstYI RGATCY 2 cut(s) 59, 886
Bsu36I CCTNAGG 1 cut(s) 914
BsuRI GGCC 8 cut(s) 76, 140, 263, 497, 529, 535, 671, 954
BtgI CCRYGG 3 cut(s) 71, 411, 459
BtsCI GGATG 3 cut(s) 803, 1261, 1449
BtsIMutI CAGTG 3 cut(s) 861, 1194, 1287
Cac8I GCNNGC 5 cut(s) 537, 648, 942, 1029, 1527
CaiI CAGNNNCTG 1 cut(s) 1286
CfoI GCGC 3 cut(s) 109, 608, 1525
Cfr10I RCCGGY 2 cut(s) 535, 1131
Cfr13I GGNCC 7 cut(s) 25, 75, 133, 527, 533, 796, 1365
Cfr42I CCGCGG 2 cut(s) 74, 414
CseI GACGC 1 cut(s) 953
Csp6I GTAC 3 cut(s) 418, 424, 1577
CspCI CAANNNNNGTGG 2 cut(s) 707, 742
CviAII CATG 7 cut(s) 199, 226, 265, 460, 1118, 1543, 1640
CviQI GTAC 3 cut(s) 418, 424, 1577
DpnI GATC 4 cut(s) 61, 279, 888, 1347
DpnII GATC 4 cut(s) 59, 277, 886, 1345
DrdI GACNNNNNNGTC 2 cut(s) 16, 440
DseDI GACNNNNNNGTC 2 cut(s) 16, 440
EaeI YGGCCR 2 cut(s) 669, 952
Eam1104I CTCTTC 5 cut(s) 183, 200, 497, 966, 1386
EarI CTCTTC 5 cut(s) 183, 200, 497, 966, 1386
EciI GGCGGA 3 cut(s) 29, 397, 535
Ecl136II GAGCTC 3 cut(s) 193, 987, 1114
Eco130I CCWWGG 3 cut(s) 381, 459, 1361
Eco24I GRGCYC 3 cut(s) 195, 989, 1116
Eco31I GGTCTC 3 cut(s) 590, 1049, 1163
Eco32I GATATC 1 cut(s) 1402
Eco47I GGWCC 4 cut(s) 25, 133, 796, 1365
Eco53kI GAGCTC 3 cut(s) 193, 987, 1114
Eco81I CCTNAGG 1 cut(s) 914
Eco88I CYCGRG 1 cut(s) 1379
EcoICRI GAGCTC 3 cut(s) 193, 987, 1114
EcoRII CCWGG 4 cut(s) 135, 140, 529, 1173
EcoRV GATATC 1 cut(s) 1402
EcoT14I CCWWGG 3 cut(s) 381, 459, 1361
EcoT38I GRGCYC 3 cut(s) 195, 989, 1116
ErhI CCWWGG 3 cut(s) 381, 459, 1361
Esp3I CGTCTC 3 cut(s) 187, 465, 576
FaeI CATG 7 cut(s) 202, 229, 268, 463, 1121, 1546, 1643
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 1 cut(s) 694
FatI CATG 7 cut(s) 198, 225, 264, 459, 1117, 1542, 1639
FblI GTMKAC 2 cut(s) 8, 231
Fnu4HI GCNGC 7 cut(s) 68, 71, 74, 99, 411, 414, 554
FokI GGATG 3 cut(s) 790, 1268, 1456
FriOI GRGCYC 3 cut(s) 195, 989, 1116
Fsp4HI GCNGC 7 cut(s) 68, 71, 74, 99, 411, 414, 554
FspBI CTAG 4 cut(s) 387, 647, 1032, 1500
GlaI GCGC 3 cut(s) 108, 607, 1524
GluI GCNGC 7 cut(s) 68, 71, 74, 99, 411, 414, 554
GsuI CTGGAG 2 cut(s) 630, 1196
HaeII RGCGCY 2 cut(s) 110, 609
HaeIII GGCC 8 cut(s) 76, 140, 263, 497, 529, 535, 671, 954
HapII CCGG 4 cut(s) 78, 536, 872, 1132
HgaI GACGC 1 cut(s) 953
HhaI GCGC 3 cut(s) 109, 608, 1525
Hin1II CATG 7 cut(s) 202, 229, 268, 463, 1121, 1546, 1643
Hin6I GCGC 3 cut(s) 107, 606, 1523
HinP1I GCGC 3 cut(s) 107, 606, 1523
HincII GTYRAC 2 cut(s) 9, 490
HindII GTYRAC 2 cut(s) 9, 490
HindIII AAGCTT 1 cut(s) 749
HpaII CCGG 4 cut(s) 78, 536, 872, 1132
HphI GGTGA 1 cut(s) 635
Hpy166II GTNNAC 5 cut(s) 9, 25, 232, 424, 490
Hpy188I TCNGA 3 cut(s) 187, 433, 781
Hpy8I GTNNAC 5 cut(s) 9, 25, 232, 424, 490
Hpy99I CGWCG 5 cut(s) 13, 89, 308, 361, 521
HpyAV CCTTC 2 cut(s) 97, 121
HpyCH4III ACNGT 3 cut(s) 479, 1225, 1422
HpyCH4IV ACGT 6 cut(s) 180, 420, 483, 1326, 1459, 1468
HpyCH4V TGCA 6 cut(s) 208, 217, 847, 1098, 1616, 1626
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 545, 865, 1003
HpySE526I ACGT 6 cut(s) 180, 420, 483, 1326, 1459, 1468
Hsp92II CATG 7 cut(s) 202, 229, 268, 463, 1121, 1546, 1643
HspAI GCGC 3 cut(s) 107, 606, 1523
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
KspI CCGCGG 2 cut(s) 74, 414
Kzo9I GATC 4 cut(s) 59, 277, 886, 1345
LguI GCTCTTC 4 cut(s) 183, 200, 497, 966
LmnI GCTCC 4 cut(s) 358, 434, 1119, 1520
LweI GCATC 5 cut(s) 90, 204, 941, 1374, 1434
MaeI CTAG 4 cut(s) 387, 647, 1032, 1500
MaeII ACGT 6 cut(s) 180, 420, 483, 1326, 1459, 1468
MaeIII GTNAC 3 cut(s) 479, 1193, 1353
MalI GATC 4 cut(s) 61, 279, 888, 1347
MbiI CCGCTC 1 cut(s) 429
MboI GATC 4 cut(s) 59, 277, 886, 1345
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 2 cut(s) 59, 886
MhlI GDGCHC 3 cut(s) 195, 989, 1116
MlsI TGGCCA 1 cut(s) 671
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 7 cut(s) 14, 26, 661, 760, 1007, 1286, 1427
MmeI TCCRAC 4 cut(s) 381, 456, 876, 1377
Mox20I TGGCCA 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 535
MroXI GAANNNNTTC 3 cut(s) 288, 506, 751
MscI TGGCCA 1 cut(s) 671
MseI TTAA 5 cut(s) 693, 1137, 1572, 1647, 1673
MslI CAYNNNNRTG 1 cut(s) 1223
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 2 cut(s) 73, 413
MspI CCGG 4 cut(s) 78, 536, 872, 1132
MspR9I CCNGG 5 cut(s) 78, 137, 142, 531, 1175
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 4 cut(s) 137, 142, 531, 1175
MvnI CGCG 2 cut(s) 73, 413
MwoI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 545, 865, 1003
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 1 cut(s) 78
NcoI CCATGG 1 cut(s) 459
NdeII GATC 4 cut(s) 59, 277, 886, 1345
NgoMIV GCCGGC 1 cut(s) 535
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 7 cut(s) 202, 229, 268, 463, 1121, 1546, 1643
NlaIV GGNNCC 2 cut(s) 354, 1376
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 1 cut(s) 1353
NspV TTCGAA 1 cut(s) 700
OliI CACNNNNGTG 1 cut(s) 1223
PaeR7I CTCGAG 1 cut(s) 1379
PciSI GCTCTTC 4 cut(s) 183, 200, 497, 966
PcsI WCGNNNNNNNCGW 5 cut(s) 300, 356, 365, 480, 1465
PctI GAATGC 1 cut(s) 847
PdiI GCCGGC 1 cut(s) 537
PdmI GAANNNNTTC 3 cut(s) 288, 506, 751
PfeI GAWTC 6 cut(s) 51, 112, 376, 404, 566, 737
Pfl23II CGTACG 1 cut(s) 417
PflMI CCANNNNNTGG 2 cut(s) 34, 1543
PkrI GCNGC 7 cut(s) 69, 72, 75, 100, 412, 415, 555
PleI GAGTC 7 cut(s) 13, 25, 660, 760, 1007, 1285, 1427
PpsI GAGTC 7 cut(s) 13, 25, 660, 760, 1007, 1285, 1427
Ppu21I YACGTR 4 cut(s) 421, 484, 1460, 1469
PsiI TTATAA 1 cut(s) 1128
Psp124BI GAGCTC 3 cut(s) 195, 989, 1116
Psp6I CCWGG 4 cut(s) 135, 140, 529, 1173
PspGI CCWGG 4 cut(s) 135, 140, 529, 1173
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 354, 1376
PspPI GGNCC 7 cut(s) 25, 75, 133, 527, 533, 796, 1365
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
PstNI CAGNNNCTG 1 cut(s) 1286
PsuI RGATCY 2 cut(s) 59, 886
RsaI GTAC 3 cut(s) 419, 425, 1578
RsaNI GTAC 3 cut(s) 418, 424, 1577
RseI CAYNNNNRTG 1 cut(s) 1223
SacI GAGCTC 3 cut(s) 195, 989, 1116
SacII CCGCGG 2 cut(s) 74, 414
SalI GTCGAC 1 cut(s) 7
SapI GCTCTTC 4 cut(s) 183, 200, 497, 966
SaqAI TTAA 5 cut(s) 693, 1137, 1572, 1647, 1673
SatI GCNGC 7 cut(s) 68, 71, 74, 99, 411, 414, 554
Sau3AI GATC 4 cut(s) 59, 277, 886, 1345
Sau96I GGNCC 7 cut(s) 25, 75, 133, 527, 533, 796, 1365
SchI GAGTC 7 cut(s) 14, 26, 661, 760, 1007, 1286, 1427
ScrFI CCNGG 5 cut(s) 78, 137, 142, 531, 1175
SduI GDGCHC 3 cut(s) 195, 989, 1116
SfaNI GCATC 5 cut(s) 90, 204, 941, 1374, 1434
Sfr274I CTCGAG 1 cut(s) 1379
Sfr303I CCGCGG 2 cut(s) 74, 414
SfuI TTCGAA 1 cut(s) 700
SgrBI CCGCGG 2 cut(s) 74, 414
SinI GGWCC 4 cut(s) 25, 133, 796, 1365
SlaI CTCGAG 1 cut(s) 1379
SmiMI CAYNNNNRTG 1 cut(s) 1223
SmlI CTYRAG 6 cut(s) 575, 982, 1037, 1243, 1274, 1379
SmoI CTYRAG 6 cut(s) 575, 982, 1037, 1243, 1274, 1379
SspMI CTAG 4 cut(s) 387, 647, 1032, 1500
SstI GAGCTC 3 cut(s) 195, 989, 1116
StyD4I CCNGG 5 cut(s) 76, 135, 140, 529, 1173
StyI CCWWGG 3 cut(s) 381, 459, 1361
TaaI ACNGT 3 cut(s) 479, 1225, 1422
TaiI ACGT 6 cut(s) 183, 423, 486, 1329, 1462, 1471
TatI WGTACW 1 cut(s) 1576
TauI GCSGC 7 cut(s) 70, 73, 76, 101, 413, 416, 556
TfiI GAWTC 6 cut(s) 51, 112, 376, 404, 566, 737
Tru1I TTAA 5 cut(s) 693, 1137, 1572, 1647, 1673
Tru9I TTAA 5 cut(s) 693, 1137, 1572, 1647, 1673
TscAI CASTG 3 cut(s) 868, 1194, 1287
TseFI GTSAC 1 cut(s) 1353
Tsp45I GTSAC 1 cut(s) 1353
TspDTI ATGAA 5 cut(s) 187, 281, 588, 1086, 1337
TspGWI ACGGA 2 cut(s) 345, 534
TspRI CASTG 3 cut(s) 868, 1194, 1287
Van91I CCANNNNNTGG 2 cut(s) 34, 1543
VpaK11BI GGWCC 4 cut(s) 25, 133, 796, 1365
XapI RAATTY 2 cut(s) 1074, 1675
XhoI CTCGAG 1 cut(s) 1379
XmiI GTMKAC 2 cut(s) 8, 231
XmnI GAANNNNTTC 3 cut(s) 288, 506, 751
XspI CTAG 4 cut(s) 387, 647, 1032, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.