Rroxscaffold_2G00145260

Plant intracellular ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006680
Physical Location & Seq
Reverse (-)
83139067 .. 83141713
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145260.1

Sequence Viewer

Length: 1704 bp
ATGGAGTCGACGGCGGAGTCGGTGGACCAAGTGGTGGAGGAGATTATGAGAATCCACAGATCCTTGCCGCCGCGGCCAGGCATCGACGAGGTGGAGGCGGCGAAGGCGCTGATTCAAAATGTGGAGAAGGAGGACCAGGCCAGGCTGGAAGCCATTGCTAAACAGACCAAGAGCAACAACGTCTCCGAAGAGCTCTTCATGGTTTTGCAGGAGATGCAGAAGAACATGGTCTACTTCAACAGCAGAGAGCAGAAGCAGGAGGCCTTGAAGTTGCTCGATCTTGAAAATCTTCACGAATTGTTCGACGATTATATCCAGAGAGCTTCCACCTGCGTTTCTTCTTCTTCTTCTTCTTCCGCTCCGTCGTCTTACACGAAGTCGATTCCCAAGGCTAGACCTAAGCCGTTGGATTCCGCCGCGGCGTACGTGTACCGCTCCGACAGAGAAGTCGGTAAGGCTAAAGCCATGGTCAGCCGAGACGACAGTTACGTGGTCAACAAGGCCAAGAAGAGCTTCTACGCCGACGGAATAGGGCCTGGGCCGGCGGTTTCCGCCACGCCGCAGATTGCCGATTCGTCTCTCAAGTCTGGTCTCGCTCTTGCTTCATCTGGAGCTGGAGCTGGAGCTGGAGTGTTAGGGTTAGGTCAAGAGGGTGAGAAATTGAGTTTGATAAAGCTAGCGAGTCTAATTGAAGTCTTGGCCAAGAAAGGGACTAAGGAGGTTAATCTTCGAAACAAGTTGAATGACCAAATCGAGTGGCTGCCTGATTCGATAGGAAAGCTTTCGAGTTTGCTGACTCTTGATTTGTCTGAAAATCGGCTTTTGGTCCTGCCATCCACCATTGGAGGACTTTCGTCCTTGACCAAATTGGATTTGCATTCCAACAGGCTCACTGAGCTACCGGACACCATTGGGGATCTTCTTAGCCTTGTCCATCTTGACCTTAGGGCAAACAATTTGGCATCTCTGCCTGCTACATTTGGCCGATTGGTGTGTCTTGAAGAGCTGGACTTGAGCTCAAATAGGCTCTCTTTGCTTCCTGACTCAATTGGGTCGCTTGCTAGTCTCAAGAGCTTGAATGTGGAGACCAATGAGATTGATGAAATTCCTCATAGCATTGGTCATTGCACGAACCTTAGAGAGCTCCATGCTGATTATAACCGGCTTAAAGCACTTCCAGAAGCCGTAGGAAAGATTGAGACCCTGGAGATTTTGTCCGTGCGTTACAATAACATCAAGCAGTTGCCCACAACCGTGTCATCTCTACTGAGCTTGAGGGAACTGGATGTGAGTTTCAATGAGCTTGAGTCAGTGCCTGAGAACTTATGTTTTGCCACCTCGCTGGTCAAGATGAACGTAGGAAACAATTTTGCTGATCTGCGGTCGCTACCAAGGTCCATAGGGAACCTCGAGATGCTTGAAGAGTTGGATATCAGCAATAACCAGATACGGTTTCTCCCCGACTCTTTCAGGATGCTCACACGACTACGTGTTCTACGTGTAGAAGAAAACCCTCTTGAAGTGCCTCCTAGACATATAGCTGACAAGGGAGCGCAAGCTGTTGTCCAATACATGGCAGAGCTTGTCGAGAAAAGAGAAGTTAAAGTACAACCGACTAAGCAAAAAAAGAGTTGGGCTCAAATCTGCTGCTTCTCAAGGTCAAACAAAAGGAAGCGCAATGGTGCAGATTATGTGAAAGCCTGA

Protein Analysis

567

Amino Acids

62.51

Weight (kDa)

6.05

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 249 - 314 7.8e-10 Leucine-rich repeat region
LRR_8 PF13855 256 - 297 1.3e-06 Leucine rich repeat
LRR_8 PF13855 286 - 343 8.9e-11 Leucine rich repeat
LRR_14 PF23598 290 - 386 3e-13 Leucine-rich repeat region
LRR_8 PF13855 447 - 506 3.6e-06 Leucine rich repeat
LRR_14 PF23598 460 - 504 1.6e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1158
AarI CACCTGC 1 cut(s) 338
AasI GACNNNNNNGTC 2 cut(s) 16, 446
Acc36I ACCTGC 1 cut(s) 338
AccB7I CCANNNNNTGG 2 cut(s) 34, 1573
AccBSI CCGCTC 2 cut(s) 359, 435
AccI GTMKAC 2 cut(s) 8, 231
AccII CGCG 2 cut(s) 73, 419
AclWI GGATC 2 cut(s) 54, 924
AcoI YGGCCR 3 cut(s) 74, 699, 982
AcsI RAATTY 1 cut(s) 1104
AfaI GTAC 3 cut(s) 425, 431, 1608
AfiI CCNNNNNNNGG 4 cut(s) 34, 77, 708, 1573
AflIII ACRYGT 3 cut(s) 426, 1489, 1498
AjnI CCWGG 5 cut(s) 76, 135, 140, 535, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AleI CACNNNNGTG 1 cut(s) 1253
Alw21I GWGCWC 3 cut(s) 195, 1019, 1146
Alw26I GTCTC 7 cut(s) 187, 471, 582, 596, 1070, 1079, 1193
AlwI GGATC 2 cut(s) 54, 924
AlwNI CAGNNNCTG 1 cut(s) 1316
Ama87I CYCGRG 1 cut(s) 1409
AoxI GGCC 8 cut(s) 74, 138, 261, 501, 533, 539, 699, 982
ApeKI GCWGC 2 cut(s) 760, 1647
ApoI RAATTY 1 cut(s) 1104
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
Asp700I GAANNNNTTC 3 cut(s) 288, 512, 781
AspLEI GCGC 3 cut(s) 109, 1555, 1677
AspS9I GGNCC 6 cut(s) 25, 133, 533, 539, 826, 1395
AsuHPI GGTGA 1 cut(s) 665
AsuII TTCGAA 1 cut(s) 730
AsuNHI GCTAGC 1 cut(s) 676
AvaI CYCGRG 1 cut(s) 1409
AvaII GGWCC 4 cut(s) 25, 133, 826, 1395
AxyI CCTNAGG 1 cut(s) 944
BalI TGGCCA 1 cut(s) 701
BanII GRGCYC 4 cut(s) 195, 1019, 1146, 1639
BarI GAAGNNNNNNTAC 4 cut(s) 500, 532, 1590, 1622
Bbv12I GWGCWC 3 cut(s) 195, 1019, 1146
BbvI GCAGC 2 cut(s) 747, 1634
BccI CCATC 2 cut(s) 841, 942
BceAI ACGGC 3 cut(s) 27, 388, 1169
BciT130I CCWGG 5 cut(s) 78, 137, 142, 537, 1205
BcoDI GTCTC 7 cut(s) 187, 471, 582, 596, 1070, 1079, 1193
BfaI CTAG 4 cut(s) 393, 677, 1062, 1530
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 338
BglI GCCNNNNNGGC 1 cut(s) 73
BisI GCNGC 9 cut(s) 68, 71, 74, 99, 417, 420, 560, 761, 1648
BlsI GCNGC 9 cut(s) 69, 72, 75, 100, 418, 421, 561, 762, 1649
Bme1390I CCNGG 5 cut(s) 78, 137, 142, 537, 1205
Bme18I GGWCC 4 cut(s) 25, 133, 826, 1395
BmeT110I CYCGRG 1 cut(s) 1409
BmgT120I GGNCC 6 cut(s) 25, 133, 533, 539, 826, 1395
BmiI GGNNCC 1 cut(s) 1406
BmrFI CCNGG 5 cut(s) 78, 137, 142, 537, 1205
BmsI GCATC 5 cut(s) 90, 204, 971, 1404, 1464
BmtI GCTAGC 1 cut(s) 680
BoxI GACNNNNGTC 1 cut(s) 853
BplI GAGNNNNNCTC 2 cut(s) 1621, 1653
BpmI CTGGAG 5 cut(s) 630, 636, 642, 648, 1226
Bpu10I CCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 6 cut(s) 566, 1033, 1052, 1294, 1325, 1639
BsaAI YACGTR 4 cut(s) 427, 490, 1490, 1499
BsaI GGTCTC 3 cut(s) 596, 1079, 1193
BsaJI CCNNGG 7 cut(s) 71, 387, 417, 465, 536, 1203, 1391
BsaWI WCCGGW 1 cut(s) 901
BsaXI ACNNNNNCTCC 8 cut(s) 167, 197, 615, 645, 1199, 1229, 1440, 1470
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 77, 708, 1573
Bse118I RCCGGY 2 cut(s) 541, 1161
Bse1I ACTGG 1 cut(s) 1287
Bse21I CCTNAGG 1 cut(s) 944
Bse3DI GCAATG 3 cut(s) 153, 1123, 1684
BseBI CCWGG 5 cut(s) 78, 137, 142, 537, 1205
BseDI CCNNGG 7 cut(s) 71, 387, 417, 465, 536, 1203, 1391
BseGI GGATG 3 cut(s) 833, 1291, 1479
BseLI CCNNNNNNNGG 4 cut(s) 34, 77, 708, 1573
BseMI GCAATG 3 cut(s) 153, 1123, 1684
BseMII CTCAG 3 cut(s) 885, 1259, 1308
BseNI ACTGG 1 cut(s) 1287
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 747, 1634
BsgI GTGCAG 1 cut(s) 1704
Bsh1236I CGCG 2 cut(s) 73, 419
Bsh1285I CGRYCG 1 cut(s) 1385
BshFI GGCC 8 cut(s) 76, 140, 263, 503, 535, 541, 701, 984
BsiEI CGRYCG 1 cut(s) 1385
BsiHKAI GWGCWC 3 cut(s) 195, 1019, 1146
BsiHKCI CYCGRG 1 cut(s) 1409
BsiSI CCGG 3 cut(s) 542, 902, 1162
BsiWI CGTACG 1 cut(s) 423
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 4 cut(s) 34, 77, 708, 1573
BsmAI GTCTC 7 cut(s) 187, 471, 582, 596, 1070, 1079, 1193
BsmBI CGTCTC 3 cut(s) 187, 471, 582
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 8 cut(s) 76, 140, 263, 503, 535, 541, 701, 984
Bso31I GGTCTC 3 cut(s) 596, 1079, 1193
BsoBI CYCGRG 1 cut(s) 1409
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 195, 1019, 1146, 1639
Bsp143I GATC 4 cut(s) 59, 277, 916, 1375
Bsp19I CCATGG 1 cut(s) 465
BspANI GGCC 8 cut(s) 76, 140, 263, 503, 535, 541, 701, 984
BspCNI CTCAG 3 cut(s) 886, 1260, 1309
BspFNI CGCG 2 cut(s) 73, 419
BspLI GGNNCC 1 cut(s) 1406
BspMI ACCTGC 1 cut(s) 338
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 2 cut(s) 54, 924
BspQI GCTCTTC 4 cut(s) 183, 200, 503, 996
BspT104I TTCGAA 1 cut(s) 730
BspTNI GGTCTC 3 cut(s) 596, 1079, 1193
BsrBI CCGCTC 2 cut(s) 359, 435
BsrDI GCAATG 3 cut(s) 153, 1123, 1684
BsrFI RCCGGY 2 cut(s) 541, 1161
BsrI ACTGG 1 cut(s) 1287
BssAI RCCGGY 2 cut(s) 541, 1161
BssECI CCNNGG 7 cut(s) 71, 387, 417, 465, 536, 1203, 1391
BssMI GATC 4 cut(s) 59, 277, 916, 1375
BssT1I CCWWGG 3 cut(s) 387, 465, 1391
Bst2UI CCWGG 5 cut(s) 78, 137, 142, 537, 1205
Bst4CI ACNGT 3 cut(s) 485, 1255, 1452
Bst6I CTCTTC 5 cut(s) 183, 200, 503, 996, 1416
BstAPI GCANNNNNTGC 1 cut(s) 214
BstBAI YACGTR 4 cut(s) 427, 490, 1490, 1499
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 5 cut(s) 543, 678, 972, 1059, 1557
BstDEI CTNAG 9 cut(s) 399, 714, 894, 923, 944, 1136, 1268, 1317, 1617
BstDSI CCRYGG 3 cut(s) 71, 417, 465
BstF5I GGATG 3 cut(s) 833, 1291, 1479
BstFNI CGCG 2 cut(s) 73, 419
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 3 cut(s) 109, 1555, 1677
BstKTI GATC 4 cut(s) 62, 280, 919, 1378
BstMAI GTCTC 7 cut(s) 187, 471, 582, 596, 1070, 1079, 1193
BstMBI GATC 4 cut(s) 59, 277, 916, 1375
BstMCI CGRYCG 1 cut(s) 1385
BstMWI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 551, 895, 1033
BstNI CCWGG 5 cut(s) 78, 137, 142, 537, 1205
BstPAI GACNNNNGTC 1 cut(s) 853
BstSCI CCNGG 5 cut(s) 76, 135, 140, 535, 1203
BstUI CGCG 2 cut(s) 73, 419
BstV1I GCAGC 2 cut(s) 747, 1634
BstX2I RGATCY 2 cut(s) 59, 916
BstXI CCANNNNNNTGG 1 cut(s) 1342
BstYI RGATCY 2 cut(s) 59, 916
Bsu36I CCTNAGG 1 cut(s) 944
BsuRI GGCC 8 cut(s) 76, 140, 263, 503, 535, 541, 701, 984
BtgI CCRYGG 3 cut(s) 71, 417, 465
BtsCI GGATG 3 cut(s) 833, 1291, 1479
BtsIMutI CAGTG 2 cut(s) 891, 1317
BveI ACCTGC 1 cut(s) 338
Cac8I GCNNGC 5 cut(s) 543, 678, 972, 1059, 1557
CaiI CAGNNNCTG 1 cut(s) 1316
CfoI GCGC 3 cut(s) 109, 1555, 1677
Cfr10I RCCGGY 2 cut(s) 541, 1161
Cfr13I GGNCC 6 cut(s) 25, 133, 533, 539, 826, 1395
Cfr42I CCGCGG 2 cut(s) 74, 420
Csp6I GTAC 3 cut(s) 424, 430, 1607
CspCI CAANNNNNGTGG 2 cut(s) 737, 772
CviAII CATG 5 cut(s) 199, 226, 466, 1148, 1573
CviQI GTAC 3 cut(s) 424, 430, 1607
DdeI CTNAG 9 cut(s) 399, 714, 894, 923, 944, 1136, 1268, 1317, 1617
DpnI GATC 4 cut(s) 61, 279, 918, 1377
DpnII GATC 4 cut(s) 59, 277, 916, 1375
DrdI GACNNNNNNGTC 2 cut(s) 16, 446
DseDI GACNNNNNNGTC 2 cut(s) 16, 446
EaeI YGGCCR 3 cut(s) 74, 699, 982
Eam1104I CTCTTC 5 cut(s) 183, 200, 503, 996, 1416
EarI CTCTTC 5 cut(s) 183, 200, 503, 996, 1416
EciI GGCGGA 3 cut(s) 29, 403, 541
Ecl136II GAGCTC 3 cut(s) 193, 1017, 1144
Eco130I CCWWGG 3 cut(s) 387, 465, 1391
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 4 cut(s) 195, 1019, 1146, 1639
Eco31I GGTCTC 3 cut(s) 596, 1079, 1193
Eco32I GATATC 1 cut(s) 1432
Eco47I GGWCC 4 cut(s) 25, 133, 826, 1395
Eco53kI GAGCTC 3 cut(s) 193, 1017, 1144
Eco81I CCTNAGG 1 cut(s) 944
Eco88I CYCGRG 1 cut(s) 1409
EcoICRI GAGCTC 3 cut(s) 193, 1017, 1144
EcoO109I RGGNCCY 1 cut(s) 533
EcoRII CCWGG 5 cut(s) 76, 135, 140, 535, 1203
EcoRV GATATC 1 cut(s) 1432
EcoT14I CCWWGG 3 cut(s) 387, 465, 1391
EcoT38I GRGCYC 4 cut(s) 195, 1019, 1146, 1639
ErhI CCWWGG 3 cut(s) 387, 465, 1391
Esp3I CGTCTC 3 cut(s) 187, 471, 582
FaeI CATG 5 cut(s) 202, 229, 469, 1151, 1576
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 1 cut(s) 724
FatI CATG 5 cut(s) 198, 225, 465, 1147, 1572
FblI GTMKAC 2 cut(s) 8, 231
Fnu4HI GCNGC 9 cut(s) 68, 71, 74, 99, 417, 420, 560, 761, 1648
FokI GGATG 3 cut(s) 820, 1298, 1486
FriOI GRGCYC 4 cut(s) 195, 1019, 1146, 1639
Fsp4HI GCNGC 9 cut(s) 68, 71, 74, 99, 417, 420, 560, 761, 1648
FspBI CTAG 4 cut(s) 393, 677, 1062, 1530
GlaI GCGC 3 cut(s) 108, 1554, 1676
GluI GCNGC 9 cut(s) 68, 71, 74, 99, 417, 420, 560, 761, 1648
GsuI CTGGAG 5 cut(s) 630, 636, 642, 648, 1226
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 8 cut(s) 76, 140, 263, 503, 535, 541, 701, 984
HapII CCGG 3 cut(s) 542, 902, 1162
HhaI GCGC 3 cut(s) 109, 1555, 1677
Hin1II CATG 5 cut(s) 202, 229, 469, 1151, 1576
Hin6I GCGC 3 cut(s) 107, 1553, 1675
HinP1I GCGC 3 cut(s) 107, 1553, 1675
HincII GTYRAC 2 cut(s) 9, 496
HindII GTYRAC 2 cut(s) 9, 496
HindIII AAGCTT 1 cut(s) 779
HpaII CCGG 3 cut(s) 542, 902, 1162
HphI GGTGA 1 cut(s) 665
Hpy166II GTNNAC 5 cut(s) 9, 25, 232, 430, 496
Hpy188I TCNGA 3 cut(s) 187, 439, 811
Hpy8I GTNNAC 5 cut(s) 9, 25, 232, 430, 496
Hpy99I CGWCG 5 cut(s) 13, 89, 308, 367, 527
HpyAV CCTTC 2 cut(s) 97, 121
HpyCH4III ACNGT 3 cut(s) 485, 1255, 1452
HpyCH4IV ACGT 6 cut(s) 180, 426, 489, 1356, 1489, 1498
HpyCH4V TGCA 5 cut(s) 208, 217, 877, 1128, 1685
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 551, 895, 1033
HpyF3I CTNAG 9 cut(s) 399, 714, 894, 923, 944, 1136, 1268, 1317, 1617
HpySE526I ACGT 6 cut(s) 180, 426, 489, 1356, 1489, 1498
Hsp92II CATG 5 cut(s) 202, 229, 469, 1151, 1576
HspAI GCGC 3 cut(s) 107, 1553, 1675
KroI GCCGGC 1 cut(s) 541
KroNI GCCGGC 1 cut(s) 543
KspI CCGCGG 2 cut(s) 74, 420
Kzo9I GATC 4 cut(s) 59, 277, 916, 1375
LguI GCTCTTC 4 cut(s) 183, 200, 503, 996
LmnI GCTCC 7 cut(s) 364, 440, 611, 617, 623, 1149, 1550
Lsp1109I GCAGC 2 cut(s) 747, 1634
LweI GCATC 5 cut(s) 90, 204, 971, 1404, 1464
MaeI CTAG 4 cut(s) 393, 677, 1062, 1530
MaeII ACGT 6 cut(s) 180, 426, 489, 1356, 1489, 1498
MaeIII GTNAC 2 cut(s) 485, 1223
MalI GATC 4 cut(s) 61, 279, 918, 1377
MbiI CCGCTC 2 cut(s) 359, 435
MboI GATC 4 cut(s) 59, 277, 916, 1375
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 2 cut(s) 59, 916
MhlI GDGCHC 4 cut(s) 195, 1019, 1146, 1639
MlsI TGGCCA 1 cut(s) 701
MluCI AATT 8 cut(s) 296, 659, 687, 866, 955, 1047, 1104, 1366
MluNI TGGCCA 1 cut(s) 701
MlyI GAGTC 7 cut(s) 14, 26, 691, 790, 1037, 1316, 1457
MmeI TCCRAC 4 cut(s) 387, 462, 906, 1407
Mox20I TGGCCA 1 cut(s) 701
MroNI GCCGGC 1 cut(s) 541
MroXI GAANNNNTTC 3 cut(s) 288, 512, 781
MscI TGGCCA 1 cut(s) 701
MseI TTAA 3 cut(s) 723, 1167, 1602
MslI CAYNNNNRTG 1 cut(s) 1253
Msp20I TGGCCA 1 cut(s) 701
MspA1I CMGCKG 2 cut(s) 73, 419
MspI CCGG 3 cut(s) 542, 902, 1162
MspR9I CCNGG 5 cut(s) 78, 137, 142, 537, 1205
MunI CAATTG 1 cut(s) 1047
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 5 cut(s) 78, 137, 142, 537, 1205
MvnI CGCG 2 cut(s) 73, 419
MwoI GCNNNNNNNGC 6 cut(s) 73, 104, 214, 551, 895, 1033
NaeI GCCGGC 1 cut(s) 543
NcoI CCATGG 1 cut(s) 465
NdeII GATC 4 cut(s) 59, 277, 916, 1375
NgoMIV GCCGGC 1 cut(s) 541
NheI GCTAGC 1 cut(s) 676
NlaIII CATG 5 cut(s) 202, 229, 469, 1151, 1576
NlaIV GGNNCC 1 cut(s) 1406
NmeAIII GCCGAG 1 cut(s) 500
NspV TTCGAA 1 cut(s) 730
OliI CACNNNNGTG 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 1409
PaqCI CACCTGC 1 cut(s) 338
PceI AGGCCT 1 cut(s) 263
PciSI GCTCTTC 4 cut(s) 183, 200, 503, 996
PcsI WCGNNNNNNNCGW 4 cut(s) 300, 371, 486, 1495
PctI GAATGC 1 cut(s) 877
PdiI GCCGGC 1 cut(s) 543
PdmI GAANNNNTTC 3 cut(s) 288, 512, 781
PfeI GAWTC 6 cut(s) 51, 112, 382, 410, 572, 767
Pfl23II CGTACG 1 cut(s) 423
PflMI CCANNNNNTGG 2 cut(s) 34, 1573
PkrI GCNGC 9 cut(s) 69, 72, 75, 100, 418, 421, 561, 762, 1649
PleI GAGTC 7 cut(s) 13, 25, 690, 790, 1037, 1315, 1457
PpsI GAGTC 7 cut(s) 13, 25, 690, 790, 1037, 1315, 1457
Ppu21I YACGTR 4 cut(s) 427, 490, 1490, 1499
PshAI GACNNNNGTC 1 cut(s) 853
PsiI TTATAA 1 cut(s) 1158
Psp124BI GAGCTC 3 cut(s) 195, 1019, 1146
Psp6I CCWGG 5 cut(s) 76, 135, 140, 535, 1203
PspGI CCWGG 5 cut(s) 76, 135, 140, 535, 1203
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 1406
PspPI GGNCC 6 cut(s) 25, 133, 533, 539, 826, 1395
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 2 cut(s) 59, 916
RsaI GTAC 3 cut(s) 425, 431, 1608
RsaNI GTAC 3 cut(s) 424, 430, 1607
RseI CAYNNNNRTG 1 cut(s) 1253
SacI GAGCTC 3 cut(s) 195, 1019, 1146
SacII CCGCGG 2 cut(s) 74, 420
SalI GTCGAC 1 cut(s) 7
SapI GCTCTTC 4 cut(s) 183, 200, 503, 996
SaqAI TTAA 3 cut(s) 723, 1167, 1602
SatI GCNGC 9 cut(s) 68, 71, 74, 99, 417, 420, 560, 761, 1648
Sau3AI GATC 4 cut(s) 59, 277, 916, 1375
Sau96I GGNCC 6 cut(s) 25, 133, 533, 539, 826, 1395
SchI GAGTC 7 cut(s) 14, 26, 691, 790, 1037, 1316, 1457
ScrFI CCNGG 5 cut(s) 78, 137, 142, 537, 1205
SduI GDGCHC 4 cut(s) 195, 1019, 1146, 1639
SfaNI GCATC 5 cut(s) 90, 204, 971, 1404, 1464
Sfr274I CTCGAG 1 cut(s) 1409
Sfr303I CCGCGG 2 cut(s) 74, 420
SfuI TTCGAA 1 cut(s) 730
SgrBI CCGCGG 2 cut(s) 74, 420
SinI GGWCC 4 cut(s) 25, 133, 826, 1395
SlaI CTCGAG 1 cut(s) 1409
SmiMI CAYNNNNRTG 1 cut(s) 1253
SmlI CTYRAG 7 cut(s) 581, 1012, 1067, 1273, 1304, 1409, 1654
SmoI CTYRAG 7 cut(s) 581, 1012, 1067, 1273, 1304, 1409, 1654
Sse9I AATT 8 cut(s) 296, 659, 687, 866, 955, 1047, 1104, 1366
SseBI AGGCCT 1 cut(s) 263
SspMI CTAG 4 cut(s) 393, 677, 1062, 1530
SstI GAGCTC 3 cut(s) 195, 1019, 1146
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 5 cut(s) 76, 135, 140, 535, 1203
StyI CCWWGG 3 cut(s) 387, 465, 1391
TaaI ACNGT 3 cut(s) 485, 1255, 1452
TaiI ACGT 6 cut(s) 183, 429, 492, 1359, 1492, 1501
TasI AATT 8 cut(s) 296, 659, 687, 866, 955, 1047, 1104, 1366
TatI WGTACW 1 cut(s) 1606
TauI GCSGC 7 cut(s) 70, 73, 76, 101, 419, 422, 562
TfiI GAWTC 6 cut(s) 51, 112, 382, 410, 572, 767
Tru1I TTAA 3 cut(s) 723, 1167, 1602
Tru9I TTAA 3 cut(s) 723, 1167, 1602
TscAI CASTG 2 cut(s) 898, 1317
TseI GCWGC 2 cut(s) 760, 1647
TspDTI ATGAA 4 cut(s) 187, 594, 1116, 1367
TspGWI ACGGA 3 cut(s) 351, 540, 1207
TspRI CASTG 2 cut(s) 898, 1317
Van91I CCANNNNNTGG 2 cut(s) 34, 1573
VpaK11BI GGWCC 4 cut(s) 25, 133, 826, 1395
XapI RAATTY 1 cut(s) 1104
XhoI CTCGAG 1 cut(s) 1409
XmiI GTMKAC 2 cut(s) 8, 231
XmnI GAANNNNTTC 3 cut(s) 288, 512, 781
XspI CTAG 4 cut(s) 393, 677, 1062, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.