AT4G36390

TRAM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014389
Physical Location & Seq
Reverse (-)
17194573 .. 17197247
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36390.1

Sequence Viewer

Length: 1923 bp
ATGGCGTCTTCTTCTCTCTCGTCTCTCTCCTCAATCCTCAGTAACCCTCATGGTTGCTCTCTCTGTTTCAAAGCCTCTACACAACGTTGTTTCGCTCTTAGATTCCTCTCCTCAAAAGCAACCCATGCTTCTTCATCTTCTTCCTCTGCTCTTCTCCCTCGTTGCCGTAGCAGCACCCATCGTCTCACACAGAAACCGATCAATAGGAAGAAGGGATTTTCCTTAAATCTTTCTAGAAGCTTCTCTATTTCTCAAATTGCTAGTTCCGGTAAGTTTGATGGTCCAAGCCTCCACCAGTTCGTCTCCAACGCTCAAGCACACGCTTCTCTTACCACTCCCGAAACTGAATCTGAAAGCACACTAGATTCTGACATTGCATCAAAAGGTCGGATTTATCACGAAACATATGGATGTCAGATGAACATAAACGACATGGAGATAGTCCTTGCGATAATGAAAAACTCTGGTTATAAAGAAGTAGTGACGGATCCTGAGAGCGCAGAAGTCATTTTCGTAAACACTTGTGCTATCAGAGAAAATGCAGAGCAAAGAGTATGGCAAAGACTTAACTACTTTTGGTTCTTAAAACGAGAATGGAAAGTAAATGCTGCTACAGGAAGAGCAAAATCTCTCAAACCTCCTAAAGTTGTTGTTTTGGGATGTATGGCTGAAAGATTAAAGGACAAGATTCTAGATTCTGATAAAATGGTTGATGTTGTTTGTGGTCCTGATGCTTATAGAGATCTCCCGAGGCTATTGGAAGAAGTAGACTATGGTCAAAAAGGAATCAACACTCTTCTTTCTCTAGAAGAGACTTATGCTGACATATCCCCTGTCCGAATCTCTGAAAACTCAATTACCGCTTTTGTTTCTGTTATGAGAGGCTGCAACAACATGTGTGCTTTCTGCATTGTTCCGTTTACCAGAGGCAGAGAGCGATCACGTCCTGTAGAATCTATAATCCGCGAGGTTGGGGAGTTGTGGGAATCGGGTGTGAAAGAAGTTACGCTTTTAGGGCAAAATGTGAACAGCTATAACGATGATTCAGCTGATCGTGAGTCAGGTGCTAACTGGGAATACAGTGAGGGATTCTCGAGCCGGTGTAAAGTTAAAAACATGGGTTTGCGATTTGCTGATCTCTTGGACCGACTCTCTGTAGAGTTTCCAGAGATGCGGTTTAGGTTTACATCGCCTCATCCTAAAGATTACCCTGACGAGTTGCTGTATTTGATGAGAGATAGACATAATATCTGCAATCTCATCCATCTTCCTGCACAATCTGGTAATAGCAGAATACTAGAACAAATGCGTAGAGGTTACACCAGAGAAGCTTATTTGGATCTTGTTAAAAAGATTCGGAGTATTATACCAGATGTGGCTATAACCAGCGACTTTATAACTGGCTTCTGTGGAGAAACTGAAGAAGAGCATCAAGAAACCCTAAGCTTGGTGAGAGCAGTTGGATACGATATGGCGTATATGTTTGCATACAGCATGAGAGAAAAGACACATGCTCATCGCAACTACACAGACGACGTTCCAGAGGAGGTGAAACAGAGACGTTTAACAGAACTCATCGACGCATTCCGCGAAACAACAGGTCCGTGTTATGACTCTCAGGTAGGATCAACTCAGTTAGTTCTCGTCGAAGGACCAAACAAGAGAGCGCCTGAGACAGAACTCATTGGAAAAACTGATAAAGGACACAGAGTTTCGTTTGTCACGAAGCCTCTGTTTGATAAAGCGTGTCTTCTTGATGGTGATGATCTGAAGAGGAATCCTGGAATTGGGGATTTTGTTGAGGTTCAGATAGAGAAATCAACGAGGGCATCGTTGTTTGGGGAAGCTCTCGCGATTAGTAAAATGTCCTTGTTTCACGATGTTGGTGTCGTCGATGCTGTTGTTGCGTCTTGTGCAAGTTAA

Protein Analysis

640

Amino Acids

71.72

Weight (kDa)

6.43

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0004 PF00919 130 - 242 1.1e-24 Uncharacterized protein family UPF0004
Radical_SAM PF04055 290 - 483 1.5e-27 Radical SAM superfamily
TRAM PF01938 539 - 617 2.1e-13 TRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36390
fragaria_vesca FvH4_1g12320
malus_domestica MD02G1137900.v1.1 MD15G1251600.v1.1
prunus_persica Prupe.7G162800_v2.0.a1
pyrus_communis pycom02g10930 pycom15g22170
rosa_chinensis RchiOBHm_Chr2g0100141
rosa_laevigata RLG00000016940
rosa_multiflora Rmu_sc0018554.1_g000003
rosa_roxburghii Rroxscaffold_2G00142530
rosa_rugosa Rorug02G0086000
rosa_samantha Rh2AG134700 Rh2BG138400 Rh2CG140200 Rh2DG140400
rosa_wichuraiana Rw2G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 471, 1397
AccI GTMKAC 1 cut(s) 768
AccII CGCG 3 cut(s) 966, 1590, 1853
AciI CCGC 4 cut(s) 861, 964, 1174, 1588
AclI AACGTT 1 cut(s) 85
AclWI GGATC 4 cut(s) 482, 495, 1347, 1633
AcuI CTGAAG 2 cut(s) 1440, 1790
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 1447, 1787
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 1 cut(s) 70
AjiI CACGTC 1 cut(s) 944
AjnI CCWGG 1 cut(s) 1780
AluBI AGCT 6 cut(s) 240, 1032, 1049, 1331, 1446, 1847
AluI AGCT 6 cut(s) 240, 1032, 1049, 1331, 1446, 1847
Alw26I GTCTC 6 cut(s) 27, 188, 307, 806, 1552, 1667
AlwI GGATC 4 cut(s) 482, 495, 1347, 1633
Ama87I CYCGRG 2 cut(s) 748, 1093
ApeKI GCWGC 3 cut(s) 171, 608, 885
AspLEI GCGC 2 cut(s) 500, 1669
AspS9I GGNCC 5 cut(s) 281, 725, 1144, 1601, 1652
AsuHPI GGTGA 3 cut(s) 1462, 1561, 1772
AvaI CYCGRG 2 cut(s) 748, 1093
AvaII GGWCC 5 cut(s) 281, 725, 1144, 1601, 1652
BamHI GGATCC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 1742
BbvI GCAGC 3 cut(s) 183, 595, 872
BccI CCATC 4 cut(s) 186, 272, 1272, 1751
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 1782
BciVI GTATCC 1 cut(s) 1457
BcoDI GTCTC 6 cut(s) 27, 188, 307, 806, 1552, 1667
BfaI CTAG 6 cut(s) 234, 261, 362, 692, 806, 1298
BfmI CTRYAG 3 cut(s) 612, 948, 1155
BfoI RGCGCY 1 cut(s) 1670
BfuI GTATCC 1 cut(s) 1457
BglII AGATCT 1 cut(s) 742
BisI GCNGC 3 cut(s) 172, 609, 886
BlsI GCNGC 3 cut(s) 173, 610, 887
Bme1390I CCNGG 1 cut(s) 1782
Bme18I GGWCC 5 cut(s) 281, 725, 1144, 1601, 1652
BmeT110I CYCGRG 2 cut(s) 748, 1093
BmgBI CACGTC 1 cut(s) 944
BmgT120I GGNCC 5 cut(s) 281, 725, 1144, 1601, 1652
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 1782
BmrI ACTGGG 1 cut(s) 1081
BmsI GCATC 6 cut(s) 386, 721, 1161, 1438, 1838, 1885
BmuI ACTGGG 1 cut(s) 1081
BoxI GACNNNNGTC 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 1742
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
Bpu10I CCTNAGC 1 cut(s) 1442
BpuEI CTTGAG 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 749
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 2 cut(s) 1447, 1787
Bse118I RCCGGY 1 cut(s) 1098
Bse1I ACTGG 3 cut(s) 295, 1076, 1405
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 1782
BseDI CCNNGG 1 cut(s) 749
BseGI GGATG 4 cut(s) 416, 665, 1195, 1260
BseLI CCNNNNNNNGG 2 cut(s) 1447, 1787
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 5 cut(s) 52, 483, 1631, 1646, 1662
BseNI ACTGG 3 cut(s) 295, 1076, 1405
BseRI GAGGAG 3 cut(s) 19, 100, 1559
BseXI GCAGC 3 cut(s) 183, 595, 872
BsgI GTGCAG 1 cut(s) 1257
Bsh1236I CGCG 3 cut(s) 966, 1590, 1853
BsiHKCI CYCGRG 2 cut(s) 748, 1093
BsiSI CCGG 2 cut(s) 267, 1099
BslI CCNNNNNNNGG 2 cut(s) 1447, 1787
BsmAI GTCTC 6 cut(s) 27, 188, 307, 806, 1552, 1667
BsmBI CGTCTC 4 cut(s) 27, 188, 307, 1552
BsmI GAATGC 1 cut(s) 1583
BsoBI CYCGRG 2 cut(s) 748, 1093
Bsp143I GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
Bsp68I TCGCGA 1 cut(s) 1853
BspACI CCGC 4 cut(s) 861, 964, 1174, 1588
BspCNI CTCAG 5 cut(s) 51, 484, 1630, 1645, 1663
BspFNI CGCG 3 cut(s) 966, 1590, 1853
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 4 cut(s) 482, 495, 1347, 1633
BspQI GCTCTTC 3 cut(s) 156, 613, 1419
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 1098
BsrI ACTGG 3 cut(s) 295, 1076, 1405
BssAI RCCGGY 1 cut(s) 1098
BssECI CCNNGG 1 cut(s) 749
BssMI GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 1782
Bst4CI ACNGT 1 cut(s) 1082
Bst6I CTCTTC 6 cut(s) 156, 613, 801, 804, 1419, 1766
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 125
BstDEI CTNAG 7 cut(s) 38, 98, 492, 1442, 1617, 1632, 1671
BstF5I GGATG 4 cut(s) 416, 665, 1195, 1260
BstFNI CGCG 3 cut(s) 966, 1590, 1853
BstH2I RGCGCY 1 cut(s) 1670
BstHHI GCGC 2 cut(s) 500, 1669
BstKTI GATC 9 cut(s) 201, 490, 745, 941, 1054, 1138, 1342, 1628, 1768
BstMAI GTCTC 6 cut(s) 27, 188, 307, 806, 1552, 1667
BstMBI GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
BstMWI GCNNNNNNNGC 5 cut(s) 125, 171, 1015, 1904, 1913
BstNI CCWGG 1 cut(s) 1782
BstNSI RCATGY 2 cut(s) 898, 1514
BstPAI GACNNNNGTC 1 cut(s) 774
BstSCI CCNGG 1 cut(s) 1780
BstSFI CTRYAG 3 cut(s) 612, 948, 1155
BstUI CGCG 3 cut(s) 966, 1590, 1853
BstV1I GCAGC 3 cut(s) 183, 595, 872
BstV2I GAAGAC 1 cut(s) 1742
BstX2I RGATCY 3 cut(s) 487, 742, 1339
BstYI RGATCY 3 cut(s) 487, 742, 1339
BsuI GTATCC 1 cut(s) 1457
BtgZI GCGATG 2 cut(s) 1173, 1502
BtrI CACGTC 1 cut(s) 944
BtsCI GGATG 4 cut(s) 416, 665, 1195, 1260
BtsIMutI CAGTG 1 cut(s) 1087
BtuMI TCGCGA 1 cut(s) 1853
CfoI GCGC 2 cut(s) 500, 1669
Cfr10I RCCGGY 1 cut(s) 1098
Cfr13I GGNCC 5 cut(s) 281, 725, 1144, 1601, 1652
CseI GACGC 2 cut(s) 1589, 1896
CviAII CATG 7 cut(s) 50, 125, 433, 895, 1117, 1495, 1511
DdeI CTNAG 7 cut(s) 38, 98, 492, 1442, 1617, 1632, 1671
DpnI GATC 9 cut(s) 200, 489, 744, 940, 1053, 1137, 1341, 1627, 1767
DpnII GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
Eam1104I CTCTTC 6 cut(s) 156, 613, 801, 804, 1419, 1766
EarI CTCTTC 6 cut(s) 156, 613, 801, 804, 1419, 1766
Eco47I GGWCC 5 cut(s) 281, 725, 1144, 1601, 1652
Eco57I CTGAAG 2 cut(s) 1440, 1790
Eco88I CYCGRG 2 cut(s) 748, 1093
EcoRII CCWGG 1 cut(s) 1780
Esp3I CGTCTC 4 cut(s) 27, 188, 307, 1552
FaeI CATG 7 cut(s) 53, 128, 436, 898, 1120, 1498, 1514
FalI AAGNNNNNCTT 4 cut(s) 993, 1025, 1734, 1766
FatI CATG 7 cut(s) 49, 124, 432, 894, 1116, 1494, 1510
FauNDI CATATG 1 cut(s) 406
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 3 cut(s) 172, 609, 886
FokI GGATG 4 cut(s) 423, 672, 1182, 1247
Fsp4HI GCNGC 3 cut(s) 172, 609, 886
FspBI CTAG 6 cut(s) 234, 261, 362, 692, 806, 1298
GlaI GCGC 2 cut(s) 499, 1668
GluI GCNGC 3 cut(s) 172, 609, 886
HaeII RGCGCY 1 cut(s) 1670
HapII CCGG 2 cut(s) 267, 1099
HgaI GACGC 2 cut(s) 1589, 1896
HhaI GCGC 2 cut(s) 500, 1669
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 53, 128, 436, 898, 1120, 1498, 1514
Hin6I GCGC 2 cut(s) 498, 1667
HinP1I GCGC 2 cut(s) 498, 1667
HindIII AAGCTT 3 cut(s) 238, 1329, 1444
HpaII CCGG 2 cut(s) 267, 1099
HphI GGTGA 3 cut(s) 1462, 1561, 1772
Hpy166II GTNNAC 5 cut(s) 517, 769, 921, 1027, 1185
Hpy8I GTNNAC 5 cut(s) 517, 769, 921, 1027, 1185
Hpy99I CGWCG 4 cut(s) 1538, 1583, 1649, 1895
HpyAV CCTTC 2 cut(s) 205, 1643
HpyCH4III ACNGT 1 cut(s) 1082
HpyCH4IV ACGT 4 cut(s) 85, 943, 1536, 1561
HpyCH4V TGCA 8 cut(s) 377, 542, 888, 909, 1254, 1274, 1487, 1916
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 171, 1015, 1904, 1913
HpyF3I CTNAG 7 cut(s) 38, 98, 492, 1442, 1617, 1632, 1671
HpySE526I ACGT 4 cut(s) 85, 943, 1536, 1561
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 53, 128, 436, 898, 1120, 1498, 1514
HspAI GCGC 2 cut(s) 498, 1667
Kzo9I GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
LguI GCTCTTC 3 cut(s) 156, 613, 1419
Lsp1109I GCAGC 3 cut(s) 183, 595, 872
LweI GCATC 6 cut(s) 386, 721, 1161, 1438, 1838, 1885
MaeI CTAG 6 cut(s) 234, 261, 362, 692, 806, 1298
MaeII ACGT 4 cut(s) 85, 943, 1536, 1561
MaeIII GTNAC 5 cut(s) 41, 481, 1003, 1316, 1720
MalI GATC 9 cut(s) 200, 489, 744, 940, 1053, 1137, 1341, 1627, 1767
MboI GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
MflI RGATCY 3 cut(s) 487, 742, 1339
MluCI AATT 3 cut(s) 255, 855, 1785
MlyI GAGTC 3 cut(s) 1067, 1143, 1607
MmeI TCCRAC 3 cut(s) 330, 368, 1441
MseI TTAA 8 cut(s) 224, 567, 584, 677, 1110, 1347, 1565, 1921
MslI CAYNNNNRTG 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 1049
MspI CCGG 2 cut(s) 267, 1099
MspR9I CCNGG 1 cut(s) 1782
Mva1269I GAATGC 1 cut(s) 1583
MvaI CCWGG 1 cut(s) 1782
MvnI CGCG 3 cut(s) 966, 1590, 1853
MwoI GCNNNNNNNGC 5 cut(s) 125, 171, 1015, 1904, 1913
NdeI CATATG 1 cut(s) 406
NdeII GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
NlaIII CATG 7 cut(s) 53, 128, 436, 898, 1120, 1498, 1514
NlaIV GGNNCC 1 cut(s) 489
NmuCI GTSAC 2 cut(s) 481, 1720
NruI TCGCGA 1 cut(s) 1853
NspI RCATGY 2 cut(s) 898, 1514
PaeR7I CTCGAG 1 cut(s) 1093
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 3 cut(s) 156, 613, 1419
PcsI WCGNNNNNNNCGW 3 cut(s) 1587, 1721, 1829
PctI GAATGC 1 cut(s) 1583
PfoI TCCNGGA 1 cut(s) 1780
PkrI GCNGC 3 cut(s) 173, 610, 887
PleI GAGTC 3 cut(s) 1066, 1143, 1607
PpsI GAGTC 3 cut(s) 1066, 1143, 1607
PscI ACATGT 1 cut(s) 894
PshAI GACNNNNGTC 1 cut(s) 774
PsiI TTATAA 2 cut(s) 471, 1397
Psp1406I AACGTT 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 1780
PspGI CCWGG 1 cut(s) 1780
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 5 cut(s) 281, 725, 1144, 1601, 1652
PsuI RGATCY 3 cut(s) 487, 742, 1339
PvuII CAGCTG 1 cut(s) 1049
RruI TCGCGA 1 cut(s) 1853
RseI CAYNNNNRTG 1 cut(s) 409
SapI GCTCTTC 3 cut(s) 156, 613, 1419
SaqAI TTAA 8 cut(s) 224, 567, 584, 677, 1110, 1347, 1565, 1921
SatI GCNGC 3 cut(s) 172, 609, 886
Sau3AI GATC 9 cut(s) 198, 487, 742, 938, 1051, 1135, 1339, 1625, 1765
Sau96I GGNCC 5 cut(s) 281, 725, 1144, 1601, 1652
SchI GAGTC 3 cut(s) 1067, 1143, 1607
ScrFI CCNGG 1 cut(s) 1782
SfaNI GCATC 6 cut(s) 386, 721, 1161, 1438, 1838, 1885
SfcI CTRYAG 3 cut(s) 612, 948, 1155
Sfr274I CTCGAG 1 cut(s) 1093
SinI GGWCC 5 cut(s) 281, 725, 1144, 1601, 1652
SlaI CTCGAG 1 cut(s) 1093
SmiMI CAYNNNNRTG 1 cut(s) 409
SmlI CTYRAG 2 cut(s) 312, 1093
SmoI CTYRAG 2 cut(s) 312, 1093
Sse9I AATT 3 cut(s) 255, 855, 1785
SsiI CCGC 4 cut(s) 861, 964, 1174, 1588
SspMI CTAG 6 cut(s) 234, 261, 362, 692, 806, 1298
StyD4I CCNGG 1 cut(s) 1780
TaaI ACNGT 1 cut(s) 1082
TaiI ACGT 4 cut(s) 88, 946, 1539, 1564
TaqI TCGA 4 cut(s) 1094, 1578, 1647, 1893
TaqII GACCGA 1 cut(s) 1161
TasI AATT 3 cut(s) 255, 855, 1785
Tru1I TTAA 8 cut(s) 224, 567, 584, 677, 1110, 1347, 1565, 1921
Tru9I TTAA 8 cut(s) 224, 567, 584, 677, 1110, 1347, 1565, 1921
TscAI CASTG 1 cut(s) 1087
TseFI GTSAC 2 cut(s) 481, 1720
TseI GCWGC 3 cut(s) 171, 608, 885
Tsp45I GTSAC 2 cut(s) 481, 1720
TspDTI ATGAA 3 cut(s) 123, 434, 470
TspGWI ACGGA 3 cut(s) 500, 906, 1593
TspRI CASTG 1 cut(s) 1087
VpaK11BI GGWCC 5 cut(s) 281, 725, 1144, 1601, 1652
XbaI TCTAGA 3 cut(s) 233, 691, 805
XceI RCATGY 2 cut(s) 898, 1514
XhoI CTCGAG 1 cut(s) 1093
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 6 cut(s) 234, 261, 362, 692, 806, 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.